| 质粒名称: | GW1-PercevalHR |
|---|---|
| 出品公司: | British Biotechnology |
| 目录编号: | 49082 |
| 存储实验室: | Gary Yellen |
|
载体骨架基本信息 |
|
| 载体名称: | GW1 |
| 载体出品公司: | British Biotechnology |
| 空载体大小: | 5270bp |
| 完整质粒大小: | 7010 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PercevalHR |
| 物种来源: | Synthetic |
| 插入大小: | 1740bp |
|
文献引用方法 |
|
| 材料和方法部分: | GW1-PercevalHR was a gift from Gary Yellen (Addgene plasmid # 49082 ; http://n2t.net/addgene:49082 ; RRID:Addgene_49082) |
| 参考文献部分: | Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio. Tantama M, Martinez-Francois JR, Mongeon R, Yellen G. Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550. 10.1038/ncomms3550 PubMed 24096541 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS GW1-PercevalHR 7557 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Mammalian expression vector (CMV promoter) of PercevalHR, a
fluorescent sensor of ATP-to-ADP ratio..
ACCESSION .
VERSION .
KEYWORDS GW1-PercevalHR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7557)
AUTHORS Tantama M, Martinez-Francois JR, Mongeon R, Yellen G
TITLE Imaging energy status in live cells with a fluorescent biosensor of
the intracellular ATP-to-ADP ratio.
JOURNAL Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550.
PUBMED 24096541
REFERENCE 2 (bases 1 to 7557)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7557
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 309..688
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 689..892
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 843..863
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 889..913
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2252..2503
/codon_start=1
/product="C-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VC155
/translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
primer_bind 2440..2461
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(2564..2585)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2825..2844)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
polyA_signal 3921..4055
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3958..3977)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4012..4031
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4078..4094)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4078..4094)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4091..4113)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4102..4118
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4126..4156)
/label=lac promoter
/note="promoter for the E. coli lac operon"
primer_bind complement(4308..4325)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4479..5067)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4559..4578)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5238..6098)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5861..5880
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin complement(6155..6668)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6350..6371)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6562..6581
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6840..6858
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(6896..6918)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7018..7037
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(7105..7124)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 7269..7291
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7283..7300
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 7284..7300
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gcttatatcg tttacggggg atggcgatag acgactttgg tgacttgggc gattctgtgt
61 gtcgcaaata tcgcagtttc gatataggtg acagacgata tgaggctata tcgccgatag
121 aggcgacatc aagctggcac atggccaatg catatcgatc tatacattga atcaatattg
181 gccattagcc atattattca ttggttatat agcataaatc aatattggct attggccatt
241 gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc
301 gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
361 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
421 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
481 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
541 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
601 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
661 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
721 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
781 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
841 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
901 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
961 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
1021 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cttatgcatg
1081 ctatactgtt tttggcttgg ggtctataca cccccgcttc ctcatgttat aggtgatggt
1141 atagcttagc ctataggtgt gggttattga ccattattga ccactcccct attggtgacg
1201 atactttcca ttactaatcc ataacatggc tctttgccac aactctcttt attggctata
1261 tgccaataca ctgtccttca gagactgaca cggactctgt atttttacag gatggggtct
1321 catttattat ttacaaattc acatatacaa caccaccgtc cccagtgccc gcagttttta
1381 ttaaacataa cgtgggatct ccacgcgaat ctcgggtacg tgttccggac atgggctctt
1441 ctccggtagc ggcggagctt ctacatccga gccctgctcc catgcctcca gcgactcatg
1501 gtcgctcggc agctccttgc tcctaacagt ggaggccaga cttaggcaca gcacgatgcc
1561 caccaccacc agtgtgccgc acaaggccgt ggcggtaggg tatgtgtctg aaaatgagct
1621 cggggagcgg gcttgcaccg ctgacgcatt tggaagactt aaggcagcgg cagaagaaga
1681 tgcaggcagc tgagttgttg tgttctgata agagtcagag gtaactcccg ttgcggtgct
1741 gttaacggtg gagggcagtg tagtctgagc agtactcgtt gctgccgcgc gcgccaccag
1801 acataatagc tgacagacta acagactgtt cctttccatg ggtcttttct gcagtcaccg
1861 tccttgacac gaagcttccc ggccacagca atcgatccat gacacaacca gtgacaagca
1921 gtcccccagt gaaaccgcat ccgcatccga gtccgatacc gataaagatt ctgaatcgga
1981 gtgagtgccg cgtccgagag cgttccctgt ccacgtccac catcggcgga gcaggtgtgc
2041 ctgaggcctc tagaccacca tgaaaaaggt tgaatccatc atcaggcccg aaaagctgga
2101 gatcgttaag aaggctctct cggacgctgg atatgtgggt atgaccgtct ctgaagtcaa
2161 aggctctggc gtccagggcg gcatcttcga gaggtaccga ggaagggtgt actctgcagg
2221 ctacaacagc gacaacgtct acatcaccgc cgacaagcag aagaacggca tcaaggccaa
2281 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
2341 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcttcca
2401 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
2461 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggtt ccggaggcat
2521 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
2581 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
2641 caagctgacc ctgaagctga tctgcaccac cggcaagctg cccgtgccct ggcccaccct
2701 cgtgaccacc ctgggctacg gtctccaatg cttcgcccgc taccccgacc acatgaagca
2761 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
2821 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
2881 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
2941 gcttgagtac aacggcacca tagtagatct gatccctaag gtaaaaattg aactagtggt
3001 gaaggaggag gacgtcgata acgtgataga cattatttgc gaaaatgccc gcacaggtaa
3061 ccccggcgac ggtaagatat ttgtgatccc agtggagcga gtggtcaggg tgcgaaccaa
3121 agaggaggga gcatctggtg gtggatccgg tggtggcggt gcatctggta tgaaaaaggt
3181 tgaagctatt attcgcccag agaaactgga gattgttaag aaggccctgt ccgacgccgg
3241 ctatgttggc atgaccgttt ccgaggttaa aggccgcggt gccggcggtg gcgacctgat
3301 tcctaaggtg aagatcgagc tggttgttaa ggaggaagac gttgataatg ttatcgacat
3361 tatctgcgag aacgcccgca ctggcaatcc tggtgacggc aaaatcttcg ttataccggt
3421 cgagcgaatt gtgcgagtac gaaccaaaga agagggtgcc tccggcggtg gcggtggctc
3481 cggtggcgcc agcggcatga agaaggttga ggccatcatc cgcccagaga agctggaaat
3541 cgttaagaag gccttaaacg acgacggcta cgtcggcatg acagttagcg aggttaaagg
3601 tcgcggtgcc ggtgggggtg atctgattcc taaggttaaa atcgagctcg ttgttaagga
3661 ggaggacgtt gacaacatta tcgatatcat ctgcgaaaac gctcgcaccg gcaacccagg
3721 cgacggcaag attttcgtca ttcctgttga gagagtggtg cgtccccgta cgaaggagga
3781 gggcaaggaa gcactgtgac tcgacgaatt cattgatcat aatcagccat accacatttg
3841 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
3901 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
3961 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
4021 ccaaactcat caatgtatct tatcatgtct ggatccgtac cgagctcgcg taatcatgtc
4081 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
4141 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactacatt aattgcgttg
4201 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
4261 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
4321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
4381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
4441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
4501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
4561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
4621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
4681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
4741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
4801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
4861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
4921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
4981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
5041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
5101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
5161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
5221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
5281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
5341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
5401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
5461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
5521 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
5581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
5641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
5701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
5761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
5821 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
5881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
5941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
6001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
6061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaataagcg
6121 gccgcggcca tgccggccac tagtctcgag ggccaccccg gttgataatc agaaaagccc
6181 caaaaacagg aagattgtat aagcaaatat ttaaattgta agcgttaata ttttgttaaa
6241 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
6301 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
6361 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
6421 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
6481 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
6541 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
6601 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
6661 gggcgcgtgg ggatccctcg agttattatt gaagcattta tcagggttat tgtctcatga
6721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
6781 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
6841 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
6901 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
6961 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
7021 catcagagca gattgtactg agagtgcacc atatgaagac gtcgcctcct cactacttct
7081 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagg
7141 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
7201 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
7261 agggttttcc cagtcacgac gttgtaaaac gacggccagt gcccgtgtca aggacggtga
7321 ctgcagtgaa taataaaatg tgtgtttgtc cgaaatacgc gttttgagat ttctgtcgcc
7381 gactaaattc atgtcgcgcg atagtggtgt ttatcgccga tagagatggc gatattggaa
7441 aaatcgatat ttgaaaatat ggcatattga aaatgtcgcc gatgtgagtt tctgtgtaac
7501 tgatatcgcc atttttccaa aagtgatttt tgggcatacg cgatatctgg cgatagc
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：MSCV IRES Luciferase
- 下一篇：hTLR2 flag
