| 出品公司: | Clontech |
|---|---|
| 载体名称: | pRetroX-IRES-ZsGreen1 |
| 质粒类型: | 哺乳动物细胞双顺反子逆病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5953bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | IRES-R: CCTCACATTGCCAAAAGACG |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | -- |
| 备注: | 载体表达ZsGreen绿色荧光蛋白作为筛选标志，用来构建稳表达细胞系。 |
| 产品目录号: | 632520 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 逆病毒 |
买家导航
pRetroX-IRES-ZsGreen1载体质粒基本信息
pRetroX-IRES-ZsGreen1质粒图谱载体图谱和pRetroX-IRES-ZsGreen1载体序列质粒序列多克隆位点信息
pRetroX-IRES-ZsGreen1质粒载体简介
pRetroX-IRES-ZsGreen1质粒序列载体序列
LOCUS pRetroX-IRES-ZsGreen1 5953 bp DNA circular 10-NOV-2009
COMMENT Created by Clontech Laboratories Inc.
COMMENT Cat. No. 632520
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
LTR 1..592
/label=5'\MMLV\LTR
misc_feature 662..1012
/label=Packaging\signal
intron 1075..1308
/label=Intron\containing\splice\acceptor\site
LTR 2718..3309
/label=3'\MMLV\LTR
rep_origin 4162..4281
/label=pUC\origin
CDS complement(4489..5278)
/label=Amp(R)
misc_feature 1361..1390
/label=MCS
misc_feature 1967..2662
/label=ZsGreen1
misc_feature 1392..1966
/label=IRES
misc_feature 650..655
/label=Splice\donor\site
BASE COUNT 1404 a 1586 c 1556 g 1407 t
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgggctcaat
421 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggcct cgctgttcct
541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
781 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
841 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
901 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
961 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
1021 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatacg cgtgtcgagc
1081 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
1141 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
1201 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
1261 ttttttcttc catttcagcc gtcgtgaaaa ctacccctaa aagccaaaaa aatcgaattc
1321 ccaaacttaa gcttggtacc gagctcggat ctcacgtggg cccgcggccg cagatctatc
1381 gatggatccg tgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
1441 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
1501 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
1561 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
1621 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
1681 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
1741 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tcacctcaag
1801 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
1861 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc
1921 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg cccagtccaa
1981 gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg tggacggcca
2041 caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc aggccatcaa
2101 cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt ccgccgcctt
2161 catgtacggc aaccgcgtgt tcaccgagta cccccaggac atcgtcgact acttcaagaa
2221 ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg gcgccgtgtg
2281 catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc acgagtccaa
2341 gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga ccgacaactg
2401 ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga agggcgacgt
2461 gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg acaccgtgta
2521 caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc acaagctgac
2581 ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc acgccatcgc
2641 ctccggctcc gccttgccct gagcggcttc gaggataaaa taaaagattt tatttagtct
2701 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
2761 gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
2821 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
2881 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
2941 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
3001 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
3061 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
3121 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
3181 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
3241 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
3301 tctttcattg aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
3361 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
3421 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
3481 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
3541 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
3601 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
3661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
3721 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
3781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
3841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
3901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
3961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
4021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
4081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
4141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
4201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
4261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
4321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
4381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
4441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
4501 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
4561 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
4621 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
4681 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
4741 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
4801 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
4861 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
4921 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
4981 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
5041 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
5101 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
5161 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
5221 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
5281 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
5341 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
5401 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
5461 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
5521 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
5581 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
5641 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
5701 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
5761 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
5821 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
5881 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
5941 tgccaagctt atg
//
