| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pAAV-CaMKIIa-EGFP |
| 质粒类型: | 腺病毒载体；荧光报告载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CaMKIIa |
| 载体大小: | 6092 bp |
| 5' 测序引物及序列: | ATGCTGACGAAGGCTCGCGA |
| 3' 测序引物及序列: | GCATTAAAGCAGCGTATCCACATAGC |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | EGFP |
| 克隆菌株: | Stbl3 或 NEB Stble |
| 宿主细胞（系）: | 神经细胞 |
| 备注: | -- |
| 产品目录号: | # 50469 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
买家导航
pAAV-CaMKIIa-EGFP载体质粒基本信息
pAAV-CaMKIIa-EGFP质粒图谱载体图谱和pAAV-CaMKIIa-EGFP载体序列质粒序列多克隆位点信息
pAAV-CaMKIIa-EGFP质粒载体简介
pAAV-CaMKIIa-EGFP质粒序列载体序列
LOCUS pAAV-CaMKIIa-EGFP 6092 bp DNA SYN
DEFINITION pAAV-CaMKIIa-EGFP
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-CaMKIIa-EGFP|
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6092
/organism="pAAV-CaMKIIa-EGFP"
/mol_type="other DNA"
CDS 1475..2194
/label="ORF frame 2"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
gene 1478..2191
/label="EGFP"
/gene="EGFP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
misc_feature complement(1520..1541)
/label="EGFP_N_primer"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
misc_feature 1658..1687
/label="Y66 (EGFP)"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
misc_feature 2128..2149
/label="EGFP_C_primer"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
misc_feature 2221..2808
/label="WPRE"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK*"
CDS 2309..2920
/label="ORF frame 2"
/translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
SSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
LWAASPHRYRALLERSTGGIPVTPPQCLSWPWKLPLQCPPALS*"
CDS 2322..2855
/label="ORF frame 3"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
terminator 2837..3315
/label="hGH_PA_terminator"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
rep_origin 3587..3893
/label="f1_origin"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
misc_feature 4161..4183
/label="pGEX_3_primer"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
promoter 4342..4370
/label="AmpR_promoter"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
gene 4412..5272
/label="Ampicillin"
/gene="Ampicillin"
/translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
PRIDTERCSRDLRVASL*"
CDS 4412..5272
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 5427..6046
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt
181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt
241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt
301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact
361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca
421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc
481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat
541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc
601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat
661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct
721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc
781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga
841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac
901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc
961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa
1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat
1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc
1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa
1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt
1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga
1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca
1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg
1441 ggggcagctc tagagcggta ccggatccgc caccatggtg agcaagggcg aggagctgtt
1501 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
1561 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
1621 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
1681 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
1741 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
1801 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
1861 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
1921 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
1981 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
2041 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
2101 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
2161 gatcactctc ggcatggacg agctgtacaa gtaggaattc gatatcaagc ttatcgataa
2221 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
2281 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
2341 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
2401 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
2461 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
2521 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
2581 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
2641 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
2701 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
2761 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc
2821 gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
2881 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
2941 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
3001 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
3061 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
3121 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
3181 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
3241 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
3301 cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac
3361 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
3421 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
3481 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
3541 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
3601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
3661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
3721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
3781 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
3841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
3901 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
3961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
4021 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
4081 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
4141 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
4201 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
4261 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
4321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
4381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
4441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
4501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
4561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
4621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
4681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
4741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
4801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
4861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
4921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
4981 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
5041 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
5101 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
5161 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
5221 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
5281 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
5341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
5401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
5461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
5521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
5581 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
5641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
5701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
5761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
5821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
5881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
5941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
6001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
6061 cctggccttt tgctggcctt ttgctcacat gt
//
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pAAV-CaMKIIa-EGFP质粒载体应用举例
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