pAAV-RC2

价格:1500元

联系方式:I47-825O-882O

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pAAVRC2载体基本信息

出品公司: Agilent
载体名称: pAAV-RC2,pAAVRC2
质粒类型: 腺相关病毒包装质粒
高拷贝/低拷贝: --
克隆方法: --
复制子: pUC
载体大小:
7327bp
5' 测序引物及序列: M13F:TGTAAAACGACGGCCAGT
3' 测序引物及序列: M13R:CAGGAAACAGCTATGACC
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: Stbl3
表达宿主: 哺乳细胞
备注: --
产品目录号: --

pAAVRC2载体质粒图谱和多克隆位点信息

pAAV-RC2载体图谱

pAAVRC2载体简介

pAAV-RC2是一个腺相关病毒包装质粒,携带了Cap和Rep基因,可与pAAV-MCS和pHelper质粒一起包装成腺相关病毒。

这个7.3kb的载体含有CAP和REP基因,这些基因可以产生产生传染性病毒所需的衣壳和DNA复制蛋白将该载体共转染到包装细胞系中,制备重组的复制缺陷型AAV病毒,pAAV-RC质粒含有AAV-2rep和CAP基因,分别编码复制蛋白和病毒衣壳结构蛋白。建立REP和CAP基因产物的正确表达水平是实现高滴度病毒生产的重要步骤。pAAV-RC从两个不同的启动子指导REP和CAP的表达,为每一组基因产物达到最佳表达水平。

pAAVRC2载体序列

LOCUS       pAAV-RC2                7327 bp ds-DNA     circular SYN 15-AUG-2018
DEFINITION  synthetic circular DNA
KEYWORDS    pAAV-RC2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7327)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             131..1996
                     /codon_start=1
                     /note="AAV-2 rep"
                     /translation="MPGFYEIVIKVPSDLDEHLPGISDSFVNWVAEKEWELPPDSDMDL
                     NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
                     LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP
                     ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA
                     RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP
                     DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK
                     TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV
                     RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF
                     GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST
                     SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSE
                     SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"
     CDS             2013..4220
                     /codon_start=1
                     /note="AAV-2 cap"
                     /translation="MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGL
                     VLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERL
                     KEDTSFGGNLGRAVFQAKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAG
                     QQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVG
                     NSSGNWHCDSTWMGDRVITTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGY
                     FDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTV
                     QVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFP
                     SQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQS
                     RLQFSQAGASDIRDQSRNWLPGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLV
                     NPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQY
                     GSVSTNLQRGNRQAATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMG
                     GFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRW
                     NPEIQYTSNYNKSVNVDFTVDTNGVYSEPRPIGTRYLTRNL"
     rep_origin      complement(4705..5160)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5187..5291
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             5292..6152
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6323..6911
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcgccgat atcgttaacg ccccgcgccg gccgctctag aactagtgga tcccccggaa
       61 gatcagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttctgatctg
      121 cgcagccgcc atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga
      181 gcatctgccc ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt
      241 gccgccagat tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga
      301 gaagctgcag cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct
      361 tttctttgtg caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac
      421 caccggggtg aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat
      481 tcagagaatt taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac
      541 cagaaatggc gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt
      601 gctccccaaa acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag
      661 cgcctgtttg aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc
      721 gcagacgcag gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag
      781 atcaaaaact tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac
      841 ctcggagaag cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc
      901 caactcgcgg tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac
      961 taaaaccgcc cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg
     1021 gatttataaa attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct
     1081 gggatgggcc acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac
     1141 taccgggaag accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt
     1201 aaactggacc aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg
     1261 ggaggagggg aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag
     1321 caaggtgcgc gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat
     1381 cgtcacctcc aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca
     1441 ccagcagccg ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga
     1501 ctttgggaag gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt
     1561 ggttgaggtg gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc
     1621 cagtgacgca gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac
     1681 gtcagacgcg gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca
     1741 cgtgggcatg aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc
     1801 aaatatctgc ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc
     1861 tcaacccgtt tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat
     1921 gggaaaggtg ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg
     1981 catctttgaa caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt
     2041 ggctcgagga cactctctct gaaggaataa gacagtggtg gaagctcaaa cctggcccac
     2101 caccaccaaa gcccgcagag cggcataagg acgacagcag gggtcttgtg cttcctgggt
     2161 acaagtacct cggacccttc aacggactcg acaagggaga gccggtcaac gaggcagacg
     2221 ccgcggccct cgagcacgac aaagcctacg accggcagct cgacagcgga gacaacccgt
     2281 acctcaagta caaccacgcc gacgcggagt ttcaggagcg ccttaaagaa gatacgtctt
     2341 ttgggggcaa cctcggacga gcagtcttcc aggcgaaaaa gagggttctt gaacctctgg
     2401 gcctggttga ggaacctgtt aagacggctc cgggaaaaaa gaggccggta gagcactctc
     2461 ctgtggagcc agactcctcc tcgggaaccg gaaaggcggg ccagcagcct gcaagaaaaa
     2521 gattgaattt tggtcagact ggagacgcag actcagtacc tgacccccag cctctcggac
     2581 agccaccagc agccccctct ggtctgggaa ctaatacgat ggctacaggc agtggcgcac
     2641 caatggcaga caataacgag ggcgccgacg gagtgggtaa ttcctcggga aattggcatt
     2701 gcgattccac atggatgggc gacagagtca tcaccaccag cacccgaacc tgggccctgc
     2761 ccacctacaa caaccacctc tacaaacaaa tttccagcca atcaggagcc tcgaacgaca
     2821 atcactactt tggctacagc accccttggg ggtattttga cttcaacaga ttccactgcc
     2881 acttttcacc acgtgactgg caaagactca tcaacaacaa ctggggattc cgacccaaga
     2941 gactcaactt caagctcttt aacattcaag tcaaagaggt cacgcagaat gacggtacga
     3001 cgacgattgc caataacctt accagcacgg ttcaggtgtt tactgactcg gagtaccagc
     3061 tcccgtacgt cctcggctcg gcgcatcaag gatgcctccc gccgttccca gcagacgtct
     3121 tcatggtgcc acagtatgga tacctcaccc tgaacaacgg gagtcaggca gtaggacgct
     3181 cttcatttta ctgcctggag tactttcctt ctcagatgct gcgtaccgga aacaacttta
     3241 ccttcagcta cacttttgag gacgttcctt tccacagcag ctacgctcac agccagagtc
     3301 tggaccgtct catgaatcct ctcatcgacc agtacctgta ttacttgagc agaacaaaca
     3361 ctccaagtgg aaccaccacg cagtcaaggc ttcagttttc tcaggccgga gcgagtgaca
     3421 ttcgggacca gtctaggaac tggcttcctg gaccctgtta ccgccagcag cgagtatcaa
     3481 agacatctgc ggataacaac aacagtgaat actcgtggac tggagctacc aagtaccacc
     3541 tcaatggcag agactctctg gtgaatccgg gcccggccat ggcaagccac aaggacgatg
     3601 aagaaaagtt ttttcctcag agcggggttc tcatctttgg gaagcaaggc tcagagaaaa
     3661 caaatgtgga cattgaaaag gtcatgatta cagacgaaga ggaaatcagg acaaccaatc
     3721 ccgtggctac ggagcagtat ggttctgtat ctaccaacct ccagagaggc aacagacaag
     3781 cagctaccgc agatgtcaac acacaaggcg ttcttccagg catggtctgg caggacagag
     3841 atgtgtacct tcaggggccc atctgggcaa agattccaca cacggacgga cattttcacc
     3901 cctctcccct catgggtgga ttcggactta aacaccctcc tccacagatt ctcatcaaga
     3961 acaccccggt acctgcgaat ccttcgacca ccttcagtgc ggcaaagttt gcttccttca
     4021 tcacacagta ctccacggga caggtcagcg tggagatcga gtgggagctg cagaaggaaa
     4081 acagcaaacg ctggaatccc gaaattcagt acacttccaa ctacaacaag tctgttaatg
     4141 tggactttac tgtggacact aatggcgtgt attcagagcc tcgccccatt ggcaccagat
     4201 acctgactcg taatctgtaa ttgcttgtta atcaataaac cgtttaattc gtttcagttg
     4261 aactttggtc tctgcgtatt tctttcttat ctagtttcca tggctacgta gataagtagc
     4321 atggcgggtt aatcattaac tacagcccgg gcgtttaaac agcgggcgga ggggtggagt
     4381 cgtgacgtga attacgtcat agggttaggg aggtcctgta ttagaggtca cgtgagtgtt
     4441 ttgcgacatt ttgcgacacc atgtggtctc gctggggggg ggggcccgag tgagcacgca
     4501 gggtctccat tttgaagcgg gaggtttgaa cgagcgctgg cgcgctcact ggccgtcgtt
     4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4681 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
     4741 taaatttttg ttaaatcagc tcattttttt aaccaatagg ccgaaatcgg caaaatccct
     4801 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     4861 ccactattaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     4921 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     4981 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     5041 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     5101 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     5161 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5221 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5281 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5341 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5461 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5521 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5581 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5641 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5761 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5821 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5881 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5941 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6061 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6121 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7141 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7201 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7261 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7321 acgccaa
//
      

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pAAVRC2载体应用举例

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