| 出品公司: | Agilent |
|---|---|
| 载体名称: | pAAV-RC2,pAAVRC2 |
| 质粒类型: | 腺相关病毒包装质粒 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 复制子: | pUC |
| 载体大小: |
7327bp
|
| 5' 测序引物及序列: | M13F:TGTAAAACGACGGCCAGT |
| 3' 测序引物及序列: | M13R:CAGGAAACAGCTATGACC |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | Stbl3 |
| 表达宿主: | 哺乳细胞 |
| 备注: | -- |
| 产品目录号: | -- |
买家导航
pAAV-RC2载体 pAAVRC2质粒基本信息
pAAV-RC2质粒图谱 pAAVRC2载体图谱和pAAV-RC2载体序列 pAAVRC2质粒序列多克隆位点信息
pAAV-RC2质粒 pAAVRC2载体简介
pAAV-RC2是一个腺相关病毒包装质粒，携带了Cap和Rep基因，可与pAAV-MCS和pHelper质粒一起包装成腺相关病毒。
这个7.3kb的载体含有CAP和REP基因，这些基因可以产生产生传染性病毒所需的衣壳和DNA复制蛋白将该载体共转染到包装细胞系中，制备重组的复制缺陷型AAV病毒，pAAV-RC质粒含有AAV-2rep和CAP基因，分别编码复制蛋白和病毒衣壳结构蛋白。建立REP和CAP基因产物的正确表达水平是实现高滴度病毒生产的重要步骤。pAAV-RC从两个不同的启动子指导REP和CAP的表达，为每一组基因产物达到最佳表达水平。
pAAV-RC2质粒序列 pAAVRC2载体序列
LOCUS pAAV-RC2 7327 bp ds-DNA circular SYN 15-AUG-2018
DEFINITION synthetic circular DNA
KEYWORDS pAAV-RC2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7327)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7327
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 131..1996
/codon_start=1
/note="AAV-2 rep"
/translation="MPGFYEIVIKVPSDLDEHLPGISDSFVNWVAEKEWELPPDSDMDL
NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP
ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA
RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP
DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK
TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV
RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF
GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST
SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSE
SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"
CDS 2013..4220
/codon_start=1
/note="AAV-2 cap"
/translation="MAADGYLPDWLEDTLSEGIRQWWKLKPGPPPPKPAERHKDDSRGL
VLPGYKYLGPFNGLDKGEPVNEADAAALEHDKAYDRQLDSGDNPYLKYNHADAEFQERL
KEDTSFGGNLGRAVFQAKKRVLEPLGLVEEPVKTAPGKKRPVEHSPVEPDSSSGTGKAG
QQPARKRLNFGQTGDADSVPDPQPLGQPPAAPSGLGTNTMATGSGAPMADNNEGADGVG
NSSGNWHCDSTWMGDRVITTSTRTWALPTYNNHLYKQISSQSGASNDNHYFGYSTPWGY
FDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTQNDGTTTIANNLTSTV
QVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFP
SQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTNTPSGTTTQS
RLQFSQAGASDIRDQSRNWLPGPCYRQQRVSKTSADNNNSEYSWTGATKYHLNGRDSLV
NPGPAMASHKDDEEKFFPQSGVLIFGKQGSEKTNVDIEKVMITDEEEIRTTNPVATEQY
GSVSTNLQRGNRQAATADVNTQGVLPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMG
GFGLKHPPPQILIKNTPVPANPSTTFSAAKFASFITQYSTGQVSVEIEWELQKENSKRW
NPEIQYTSNYNKSVNVDFTVDTNGVYSEPRPIGTRYLTRNL"
rep_origin complement(4705..5160)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5187..5291
/gene="bla"
/note="AmpR promoter"
CDS 5292..6152
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6323..6911
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gcgcgccgat atcgttaacg ccccgcgccg gccgctctag aactagtgga tcccccggaa
61 gatcagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttctgatctg
121 cgcagccgcc atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga
181 gcatctgccc ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt
241 gccgccagat tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga
301 gaagctgcag cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct
361 tttctttgtg caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac
421 caccggggtg aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat
481 tcagagaatt taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac
541 cagaaatggc gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt
601 gctccccaaa acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag
661 cgcctgtttg aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc
721 gcagacgcag gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag
781 atcaaaaact tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac
841 ctcggagaag cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc
901 caactcgcgg tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac
961 taaaaccgcc cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg
1021 gatttataaa attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct
1081 gggatgggcc acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac
1141 taccgggaag accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt
1201 aaactggacc aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg
1261 ggaggagggg aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag
1321 caaggtgcgc gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat
1381 cgtcacctcc aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca
1441 ccagcagccg ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga
1501 ctttgggaag gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt
1561 ggttgaggtg gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc
1621 cagtgacgca gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac
1681 gtcagacgcg gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca
1741 cgtgggcatg aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc
1801 aaatatctgc ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc
1861 tcaacccgtt tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat
1921 gggaaaggtg ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg
1981 catctttgaa caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt
2041 ggctcgagga cactctctct gaaggaataa gacagtggtg gaagctcaaa cctggcccac
2101 caccaccaaa gcccgcagag cggcataagg acgacagcag gggtcttgtg cttcctgggt
2161 acaagtacct cggacccttc aacggactcg acaagggaga gccggtcaac gaggcagacg
2221 ccgcggccct cgagcacgac aaagcctacg accggcagct cgacagcgga gacaacccgt
2281 acctcaagta caaccacgcc gacgcggagt ttcaggagcg ccttaaagaa gatacgtctt
2341 ttgggggcaa cctcggacga gcagtcttcc aggcgaaaaa gagggttctt gaacctctgg
2401 gcctggttga ggaacctgtt aagacggctc cgggaaaaaa gaggccggta gagcactctc
2461 ctgtggagcc agactcctcc tcgggaaccg gaaaggcggg ccagcagcct gcaagaaaaa
2521 gattgaattt tggtcagact ggagacgcag actcagtacc tgacccccag cctctcggac
2581 agccaccagc agccccctct ggtctgggaa ctaatacgat ggctacaggc agtggcgcac
2641 caatggcaga caataacgag ggcgccgacg gagtgggtaa ttcctcggga aattggcatt
2701 gcgattccac atggatgggc gacagagtca tcaccaccag cacccgaacc tgggccctgc
2761 ccacctacaa caaccacctc tacaaacaaa tttccagcca atcaggagcc tcgaacgaca
2821 atcactactt tggctacagc accccttggg ggtattttga cttcaacaga ttccactgcc
2881 acttttcacc acgtgactgg caaagactca tcaacaacaa ctggggattc cgacccaaga
2941 gactcaactt caagctcttt aacattcaag tcaaagaggt cacgcagaat gacggtacga
3001 cgacgattgc caataacctt accagcacgg ttcaggtgtt tactgactcg gagtaccagc
3061 tcccgtacgt cctcggctcg gcgcatcaag gatgcctccc gccgttccca gcagacgtct
3121 tcatggtgcc acagtatgga tacctcaccc tgaacaacgg gagtcaggca gtaggacgct
3181 cttcatttta ctgcctggag tactttcctt ctcagatgct gcgtaccgga aacaacttta
3241 ccttcagcta cacttttgag gacgttcctt tccacagcag ctacgctcac agccagagtc
3301 tggaccgtct catgaatcct ctcatcgacc agtacctgta ttacttgagc agaacaaaca
3361 ctccaagtgg aaccaccacg cagtcaaggc ttcagttttc tcaggccgga gcgagtgaca
3421 ttcgggacca gtctaggaac tggcttcctg gaccctgtta ccgccagcag cgagtatcaa
3481 agacatctgc ggataacaac aacagtgaat actcgtggac tggagctacc aagtaccacc
3541 tcaatggcag agactctctg gtgaatccgg gcccggccat ggcaagccac aaggacgatg
3601 aagaaaagtt ttttcctcag agcggggttc tcatctttgg gaagcaaggc tcagagaaaa
3661 caaatgtgga cattgaaaag gtcatgatta cagacgaaga ggaaatcagg acaaccaatc
3721 ccgtggctac ggagcagtat ggttctgtat ctaccaacct ccagagaggc aacagacaag
3781 cagctaccgc agatgtcaac acacaaggcg ttcttccagg catggtctgg caggacagag
3841 atgtgtacct tcaggggccc atctgggcaa agattccaca cacggacgga cattttcacc
3901 cctctcccct catgggtgga ttcggactta aacaccctcc tccacagatt ctcatcaaga
3961 acaccccggt acctgcgaat ccttcgacca ccttcagtgc ggcaaagttt gcttccttca
4021 tcacacagta ctccacggga caggtcagcg tggagatcga gtgggagctg cagaaggaaa
4081 acagcaaacg ctggaatccc gaaattcagt acacttccaa ctacaacaag tctgttaatg
4141 tggactttac tgtggacact aatggcgtgt attcagagcc tcgccccatt ggcaccagat
4201 acctgactcg taatctgtaa ttgcttgtta atcaataaac cgtttaattc gtttcagttg
4261 aactttggtc tctgcgtatt tctttcttat ctagtttcca tggctacgta gataagtagc
4321 atggcgggtt aatcattaac tacagcccgg gcgtttaaac agcgggcgga ggggtggagt
4381 cgtgacgtga attacgtcat agggttaggg aggtcctgta ttagaggtca cgtgagtgtt
4441 ttgcgacatt ttgcgacacc atgtggtctc gctggggggg ggggcccgag tgagcacgca
4501 gggtctccat tttgaagcgg gaggtttgaa cgagcgctgg cgcgctcact ggccgtcgtt
4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
4681 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
4741 taaatttttg ttaaatcagc tcattttttt aaccaatagg ccgaaatcgg caaaatccct
4801 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
4861 ccactattaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
4921 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
4981 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
5041 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
5101 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
5161 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
5221 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
5281 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
5341 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
5401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
5461 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
5521 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
5581 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
5641 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
5701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
5761 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
5821 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
5881 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
5941 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
6001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
6061 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
6121 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
6181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
6241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
6361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
6421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
6481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
6541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
6661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
6721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
6781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
6841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
6961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
7021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
7081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
7141 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
7201 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
7261 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
7321 acgccaa
//
pAAV-RC2载体图谱 pAAVRC2质粒图谱pdf版和pAAV-RC2载体序列 pAAVRC2质粒序列等相关资料下载
pAAV-RC2质粒 pAAVRC2载体应用举例
- 上一篇：pAAV-CaMKIIa-EGFP
- 下一篇：pAAV-RC6
