| 质粒名称: | pMy-BirA-T2A-eGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 50565 |
| 存储实验室: | Rafael Casellas |
|
载体骨架基本信息 |
|
| 载体名称: | pMy |
| 载体抗性: | 氨苄霉素 |
| 载体类型: | Retroviral |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Ampicilin |
|
文献引用方法 |
|
| 材料和方法部分: | pMy-BirA-T2A-eGFP was a gift from Rafael Casellas (爱迪基因 plasmid # 50565) |
| 参考文献部分: | A genome-wide map of CTCF multivalency redefines the CTCF code. Nakahashi H, Kwon KR, Resch W, Vian L, Dose M, Stavreva D, Hakim O, Pruett N, Nelson S, Yamane A, Qian J, Dubois W, Welsh S, Phair RD, Pugh BF, Lobanenkov V, Hager GL, Casellas R. Cell Rep. 2013 May 30;3(5):1678-89. doi: 10.1016/j.celrep.2013.04.024. Epub 2013 May 23. 10.1016/j.celrep.2013.04.024 PubMed 23707059 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMy-BirA-T2A-eGFP 6380 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION Retroviral vector encoding for GFP and the biotinylating enzyme BirA
.
ACCESSION .
VERSION .
KEYWORDS pMy-BirA-T2A-eGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6380)
AUTHORS Nakahashi H, Kwon KR, Resch W, Vian L, Dose M, Stavreva D, Hakim O,
Pruett N, Nelson S, Yamane A, Qian J, Dubois W, Welsh S, Phair RD,
Pugh BF, Lobanenkov V, Hager GL, Casellas R
TITLE A genome-wide map of CTCF multivalency redefines the CTCF code.
JOURNAL Cell Rep. 2013 May 30;3(5):1678-89. doi:
10.1016/j.celrep.2013.04.024. Epub 2013 May 23.
PUBMED 23707059
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pMy-BirA-T2A-eGFP|
FEATURES Location/Qualifiers
source 1..6380
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1090..1506
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1436..1458
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1474..1490
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 1516..1890
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 1828..1844
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
CDS 1905..2867
/codon_start=1
/gene="birA"
/product="E. coli biotin protein ligase"
/label=BirA
/translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
CDS 2868..2921
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 2922..3641
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2967..2988)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3228..3247)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3575..3596
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(4484..4501)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4655..5243)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4735..4754)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5407..6267)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6030..6049
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6268..6372)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
61 caaggcatgg aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaga
121 tggaacagct gaatatgggc caaagcggat atctgtggta agcagttcct gccccggctc
181 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
241 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
301 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
361 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
421 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
481 cgcccgggta cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc
541 gctgatcctt gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt
601 tggaggttcc accgagattt ggagacccca gcccagggac caccgacccc cccgccggga
661 ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc
721 atctaatgtt tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc
781 cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc ctgggagacg tcccaggtcg
841 ggggccgttt ttgtggcccg acctgagtcc aaaaagcccg atcgttttgg actctttggt
901 gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct aaaacagttc
961 ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc gcgtcttgtc
1021 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
1081 atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
1141 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
1201 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
1261 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
1321 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
1381 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
1441 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
1501 ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct tgtaaacttc
1561 cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca cttacaggct
1621 ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca agaacaactg
1681 gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt ccgccgacac
1741 cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct gaccaccccc
1801 accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt gaaggctgcc
1861 gaccccgggg gtggaccatc ctctagactg ccggatccgc caccatgaag gataacaccg
1921 tgccactgaa attgattgcc ctgttagcga acggtgaatt tcactctggc gagcagttgg
1981 gtgaaacgct gggaatgagc cgggcggcta ttaataaaca cattcagaca ctgcgtgact
2041 ggggcgttga tgtctttacc gttccgggta aaggatacag cctgcctgag cctatccagt
2101 tacttaatgc taaacagata ttgggtcagc tggatggcgg tagtgtagcc gtgctgccag
2161 tgattgactc cacgaatcag taccttcttg atcgtatcgg agagcttaaa tcgggcgatg
2221 cttgcattgc agaataccag caggctggcc gtggtcgccg gggtcggaaa tggttttcgc
2281 cttttggcgc aaacttatat ttgtcgatgt tctggcgtct ggaacaaggc ccggcggcgg
2341 cgattggttt aagtctggtt atcggtatcg tgatggcgga agtattacgc aagctgggtg
2401 cagataaagt tcgtgttaaa tggcctaatg acctctatct gcaggatcgc aagctggcag
2461 gcattctggt ggagctgact ggcaaaactg gcgatgcggc gcaaatagtc attggagccg
2521 ggatcaacat ggcaatgcgc cgtgttgaag agagtgtcgt taatcagggg tggatcacgc
2581 tgcaggaagc ggggatcaat ctcgatcgta atacgttggc ggccatgcta atacgtgaat
2641 tacgtgctgc gttggaactc ttcgaacaag aaggattggc accttatctg tcgcgctggg
2701 aaaagctgga taattttatt aatcgcccag tgaaacttat cattggtgat aaagaaatat
2761 ttggcatttc acgcggaata gacaaacagg gggctttatt acttgagcag gatggaataa
2821 taaaaccctg gatgggcggt gaaatatcct tgcgtagtgc agaaaaagag ggcagaggaa
2881 gtcttctaac atgcggtgac gtggaggaga atcccggccc tatggtgagc aagggcgagg
2941 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
3001 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
3061 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
3121 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
3181 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
3241 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
3301 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
3361 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
3421 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
3481 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
3541 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
3601 ccgccgggat cactctcggc atggacgagc tgtacaagta aactcgacat ttcgaccggg
3661 ccgccagcac agtggggggg ggcccgcgat tagtccaatt tgttaaagac aggatatcag
3721 tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc
3781 catagataga ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
3841 cctgtaggtt tggcaagcta gcttaagtaa gccattttgc aaggcatgga aaaatacata
3901 actgagaata gagaagttca gatcaaggtt aggaacagag agacaggaga atatgggcca
3961 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agttggaaca
4021 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
4081 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
4141 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
4201 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
4261 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgtatcca
4321 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
4381 ctgagtgatt gactacccgt cagcgggggt ctttcattct gcattaatga atcggccaac
4441 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
4501 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
4561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
4621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
4681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
4741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
4801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
4861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
4921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
4981 gacacgactt atagccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
5041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
5101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
5161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
5221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
5281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
5341 cctagatcct tttgcggccg gccgcaaatc aatctaaagt atatatgagt aaacttggtc
5401 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
5461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
5521 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
5581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
5641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
5701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
5761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
5821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
5881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
5941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
6001 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
6061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
6121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
6181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
6241 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
6301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
6361 aggggttccg cgcacatttc
//
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