出品人: | Lynch,M.D. and Gill,R.T. |
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载体名称: | pBTL-5 |
质粒类型: | 广宿主载体;克隆载体;原核表达载体 |
高拷贝/低拷贝: | -- |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | lac |
载体大小: | 2039 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 甲氧氨苄嘧啶 |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 多个菌属 |
备注: | -- |
产品目录号: | -- |
组成型/诱导型: | 诱导型 |
买家导航
pBTL-5载体质粒基本信息
pBTL-5质粒图谱载体图谱和pBTL-5载体序列质粒序列多克隆位点信息
pBTL-5质粒载体简介
pBTL-5质粒序列载体序列
LOCUS DQ058743 2039 bp DNA circular SYN 08-MAY-2006 DEFINITION Broad host range vector pBTL-5, complete sequence. ACCESSION DQ058743 VERSION DQ058743.1 GI:69048483 AUTHORS Lynch,M.D. and Gill,R.T. TITLE Broad host range vectors for stable genomic library construction JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering, University of Colorado, ECCH 111, Campus Box 424, Boulder, CO 80309, USA FEATURES Location/Qualifiers source 1..2039 /organism="Broad host range vector pBTL-5" /mol_type="other DNA" /db_xref="taxon:334975" regulatory 60..96 /regulatory_class="terminator" /note="SoxR terminator" gene 872..1534 /gene="rep" /note="rep gene from pBBR1, GenBank Accession Number X66730" CDS 872..1534 /gene="rep" /note="required for replication" /codon_start=1 /transl_table=11 /product="Rep" /protein_id="AAY99755.1" /db_xref="GI:69048484" /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP RR" gene 1631..1867 /gene="dhfrIIb" /note="dhfrIIb gene from R388" CDS 1631..1867 /gene="dhfrIIb" /codon_start=1 /transl_table=11 /product="dihydrofolate reductase" /protein_id="AAY99756.1" /db_xref="GI:69048485" /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVG WYCTKLTPEGYAVESESHPGSVQIYPVAALERVA" regulatory 1884..1915 /regulatory_class="terminator" /note="TonB terminator" regulatory 1942..2024 /regulatory_class="promoter" /note="lac promoter" ORIGIN 1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga 61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg 121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca 181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc 241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag 301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc 361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc 421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg 481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt 541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg 601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg 661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac 721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg 781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa 841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat 901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg 961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca 1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg 1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt 1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg 1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca 1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta 1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat 1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg 1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga 1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca 1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa 1621 ggaagctaaa atgggtcaaa gtagcgatga agccaacgct cccgttgcag ggcagtttgc 1681 gcttcccctg agtgccacct ttggcttagg ggatcgcgta cgcaagaaat ctggtgccgc 1741 ttggcagggt caagtcgtcg gttggtattg cacaaaactc actcctgaag gctatgcggt 1801 cgagtccgaa tcccacccag gctcagtgca aatttatcct gtggctgcac ttgaacgtgt 1861 ggcctaagaa tggatccccc tcaagtcaaa agcctccggt cggaggcttt tgactttctg 1921 ctatggaggt caggtatgat tttgcattag gcaccccagg ctttacactt tatgcttccg 1981 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acactctaga gaattcgtc //