| 出品人: | Lynch,M.D. and Gill,R.T. |
|---|---|
| 载体名称: | pBMTB-1 |
| 质粒类型: | 广宿主载体；克隆载体；原核表达载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | araBAD |
| 载体大小: | 5029 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 多个菌属 |
| 备注: | -- |
| 产品目录号: | -- |
| 组成型/诱导型: | 诱导型 |
买家导航
pBMTB-1载体质粒基本信息
pBMTB-1质粒图谱载体图谱和pBMTB-1载体序列质粒序列多克隆位点信息
pBMTB-1质粒载体简介
pBMTB-1质粒序列载体序列
LOCUS DQ058724 5029 bp DNA circular SYN 08-MAY-2006
DEFINITION Broad host range vector pBMTB-1, complete sequence.
ACCESSION DQ058724
VERSION DQ058724.1 GI:69048156
AUTHORS Lynch,M.D. and Gill,R.T.
TITLE Broad host range vectors for stable genomic library construction
PUBMED 16496398
JOURNAL Submitted (12-MAY-2005) Chemical & Biological Engineering,
University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
80309, USA
FEATURES Location/Qualifiers
source 1..5029
/organism="Broad host range vector pBMTB-1"
/mol_type="other DNA"
/db_xref="taxon:334956"
regulatory 60..96
/regulatory_class="terminator"
/note="SoxR terminator"
gene complement(104..1123)
/gene="mob"
/note="mob gene from pBBR1MCS, GenBank Accession Number
U02374"
CDS complement(104..1123)
/gene="mob"
/note="required for mobilization"
/codon_start=1
/transl_table=11
/product="Mob"
/protein_id="AAY99697.1"
/db_xref="GI:69048157"
/translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
KPLLS"
gene 2117..2779
/gene="rep"
/note="rep gene from pBBR1, GenBank Accession Number
X66730"
CDS 2117..2779
/gene="rep"
/note="required for replication"
/codon_start=1
/transl_table=11
/product="Rep"
/protein_id="AAY99698.1"
/db_xref="GI:69048158"
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
RR"
gene 2876..3736
/gene="bla"
/note="beta-lactam resistance"
CDS 2876..3736
/gene="bla"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAY99699.1"
/db_xref="GI:69048159"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3752..3783
/regulatory_class="terminator"
/note="TonB terminator"
gene complement(3811..4740)
/gene="araC"
CDS complement(3811..4740)
/gene="araC"
/note="regulator"
/codon_start=1
/transl_table=11
/product="AraC"
/protein_id="AAY99700.1"
/db_xref="GI:69048160"
/translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
regulatory 4748..5015
/regulatory_class="promoter"
/note="pBAD promoter"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
2881 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
2941 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
3001 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagtttac gccccgaaga
3061 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
3121 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
3181 gtactcacca gtcacagaaa agcatctcac ggatggcatg acagtaagag aattatgcag
3241 tgctgccata accatgagtg ataacactgc ggccaactta cttctggcaa cgatcggagg
3301 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
3361 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
3421 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
3481 gcaacaatta atagactgga tggaggcgga taaagttgca ggatcacttc tgcgctcggc
3541 cctcccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
3601 tatcattgca gcactggggc cagatggtaa gccctcccgc atcgtagtta tctacacgac
3661 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
3721 gattaagcat tggtaatgag gatccccctc aagtcaaaag cctccggtcg gaggcttttg
3781 actttctgct atggaggtca ggtatgattt ttatgacaac ttgacggcta catcattcac
3841 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
3901 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
3961 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa
4021 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac
4081 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
4141 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat
4201 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
4261 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
4321 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca
4381 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
4441 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa
4501 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
4561 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt
4621 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc
4681 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat
4741 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc
4801 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa
4861 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg
4921 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca
4981 actctctact gtttctccat acccgttttt ttgggctaga gaattcgtc
//
