| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.73 ; pGL4.73[hRluc/SV40] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | SV40 |
| 载体大小: | 3921 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRluc |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.73[luc2P]载体是信号通路报告载体；含海肾 荧光素酶报告基因hRluc。 |
| 产品目录号: | E6911 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.73[hRluc/SV40]载体pGL4.73质粒基本信息
pGL4.73[hRluc/SV40]质粒图谱pGL4.73载体图谱和pGL4.73[hRluc/SV40]载体序列pGL4.73质粒序列多克隆位点信息
pGL4.73[hRluc/SV40]质粒pGL4.73载体简介
pGL4.73载体简介 The pGL4.73[hRluc/SV40] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.73[hRluc/SV40] Vector contains the hRluc reporter gene and an SV40 early enhancer/promoter and can be used as an expression control or a co-reporter vector.
pGL4.73[hRluc/SV40]质粒序列pGL4.73载体序列
LOCUS AY738229 3921 bp DNA circular SYN 08-NOV-2004
DEFINITION Renilla luciferase reporter vector pGL4.73[hRluc/SV40], complete
sequence.
ACCESSION AY738229
VERSION AY738229.1 GI:55535639
KEYWORDS .
SOURCE Renilla luciferase reporter vector pGL4.73[hRluc/SV40]
ORGANISM Renilla luciferase reporter vector pGL4.73[hRluc/SV40]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3921)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3921)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2004) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..3921
/organism="Renilla luciferase reporter vector
pGL4.73[hRluc/SV40]"
/mol_type="other DNA"
/db_xref="taxon:296666"
regulatory 51..469
/regulatory_class="promoter"
/note="SV40 early"
CDS 499..1434
/codon_start=1
/transl_table=11
/product="luciferase hRluc"
/protein_id="AAV52883.1"
/db_xref="GI:55535640"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
misc_feature 1466..1687
/note="SV40 late poly(A) region"
primer_bind 1755..1774
/note="reporter vector primer 4 (RVPrimer4)"
rep_origin 2012
/note="ColE1-derived"
gene complement(2803..3663)
/gene="ampR"
CDS complement(2803..3663)
/gene="ampR"
/note="AmpR"
/codon_start=1
/transl_table=11
/product="synthetic beta-lactamase"
/protein_id="AAV52884.1"
/db_xref="GI:55535641"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3768..3921
/regulatory_class="polyA_signal_sequence"
/note="synthetic; transcriptional pause site"
primer_bind complement(3870..3889)
/note="reporter vector primer 3 (RVPrimer3)"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcagatct gcgcagcacc
61 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
121 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
181 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
301 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
361 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
421 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gcaatccggt
481 actgttggta aagccaccat ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg
541 atcactgggc ctcagtggtg ggctcgctgc aagcaaatga acgtgctgga ctccttcatc
601 aactactatg attccgagaa gcacgccgag aacgccgtga tttttctgca tggtaacgct
661 gcctccagct acctgtggag gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc
721 atccctgatc tgatcggaat gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc
781 ctggatcact acaagtacct caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc
841 atctttgtgg gccacgactg gggggcttgt ctggcctttc actactccta cgagcaccaa
901 gacaagatca aggccatcgt ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac
961 gagtggcctg acatcgagga ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg
1021 gtgcttgaga ataacttctt cgtcgagacc atgctcccaa gcaagatcat gcggaaactg
1081 gagcctgagg agttcgctgc ctacctggag ccattcaagg agaagggcga ggttagacgg
1141 cctaccctct cctggcctcg cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc
1201 cagattgtcc gcaactacaa cgcctacctt cgggccagcg acgatctgcc taagatgttc
1261 atcgagtccg accctgggtt cttttccaac gctattgtcg agggagctaa gaagttccct
1321 aacaccgagt tcgtgaaggt gaagggcctc cacttcagcc aggaggacgc tccagatgaa
1381 atgggtaagt acatcaagag cttcgtggag cgcgtgctga agaacgagca gtaattctag
1441 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
1501 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
1561 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
1621 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
1681 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag
1741 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
1801 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
1861 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
1921 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
1981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
2041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
2101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
2161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
2221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
2281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
2341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
2401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
2461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
2521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
2581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
2641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
2701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
2761 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag
2821 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt
2881 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc
2941 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc
3001 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg
3061 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac
3121 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg
3181 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc
3241 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact
3301 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc
3361 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat
3421 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc
3481 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac
3541 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa
3601 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct
3661 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca
3721 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt
3781 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
3841 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
3901 caggtgccag aacatttctc t
//
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pGL4.73[hRluc/SV40]质粒pGL4.73载体应用举例
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