质粒名称: | pDA093 |
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出品公司: | Addgene |
目录编号: | 131390 |
存储实验室: | Kathleen Burns |
载体骨架基本信息 |
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载体名称: | pSBtet-RN |
完整质粒大小: | 11444bp |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 未知 |
插入基因信息 |
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插入基因名称: | ORFeus |
物种来源: | H. sapiens (human) |
文献引用方法 |
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材料和方法部分: |
pDA093 was a gift from Kathleen Burns (Addgene plasmid # 131390 ; http://n2t.net/addgene:131390 ; RRID:Addgene_131390)
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参考文献部分: | Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication. Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, Smogorzewska A, Holland AJ, Burns KH. Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 10.1038/s41594-020-0372-1. Epub 2020 Feb 10. 10.1038/s41594-020-0372-1 PubMed 32042151 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDA093 11444 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION generation of Tet-On RPE. ACCESSION . VERSION . KEYWORDS pDA093 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11444) AUTHORS Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, Smogorzewska A, Holland AJ, Burns KH TITLE Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication. JOURNAL Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 10.1038/s41594-020-0372-1. Epub 2020 Feb 10. PUBMED 32042151 REFERENCE 2 (bases 1 to 11444) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..11444 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 56..368 /label=tight TRE promoter /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" protein_bind 64..82 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 100..118 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 135..153 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 171..189 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 207..225 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 242..260 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 278..296 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 341..365 /label=LNCX /note="Human CMV promoter, forward primer" polyA_signal 5378..5499 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5389..5408) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 5443..5462 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS 6222..6923 /codon_start=1 /product="dimeric variant of DsRed fluorescent protein (Shaner et al., 2004)" /label=dTomato /note="mammalian codon-optimized" /translation="MLSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLYGMDELYK" primer_bind complement(6355..6373) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 6623..6642 /label=mCherry-F /note="mCherry, forward primer" primer_bind 6817..6840 /label=DsRed1-C /note="DsRed1, forward primer" CDS 6933..6989 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 6990..7733 /codon_start=1 /product="improved tetracycline-controlled transactivator" /label=rtTA-Advanced /note="In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only in the presence of tetracycline or doxycycline." /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG" CDS 7740..7796 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 7797..8591 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7851..7870) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8461..8480 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(8646..8663) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8652..8876 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(9202..9219) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9373..9961) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9453..9472) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10132..10992) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10755..10774 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10993..11097) /gene="bla" /label=AmpR promoter ORIGIN 1 ttcgactcct ctgcagaatg cggcgatgtt tcggtaaggg gtccgctatc tagacgagtt 61 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac 121 gatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag 181 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt 241 atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt 301 atgtcgatta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc 361 agatcgcctg gagccaattc caactctttt gccttatacc tactttccgt accacttcct 421 accctcgaaa ggcctctgat gggcaagaag cagaaccgca agaccggcaa cagcaagacc 481 cagagcgcca gccccccccc caaggagcgc agcagcagcc ccgccaccga gcagagctgg 541 atggagaacg acttcgacga gctgcgcgag gagggcttcc gccgcagcaa ctacagcgag 601 ctgcgcgagg acatccagac caagggcaag gaggtggaga acttcgagaa gaacctggag 661 gagtgcatca cccgcatcac caacaccgag aagtgcctga aggagctgat ggagctgaag 721 accaaggccc gcgagctgcg cgaggagtgc cgcagcctgc gcagccgctg cgaccaattg 781 gaggagcgcg tgagcgccat ggaggacgag atgaacgaga tgaagcgcga gggcaagttc 841 cgcgagaagc gcatcaagcg caacgagcag agcctgcagg agatctggga ctacgtgaag 901 cgccccaacc tgcgcctgat cggcgtgccc gagagcgacg tggagaacgg caccaagctg 961 gagaacaccc tgcaggacat catccaggag aacttcccca acctggcccg ccaggccaac 1021 gtgcagatcc aggagatcca gcgcaccccc cagcgctaca gcagccgccg cgccaccccc 1081 cgccacatca tcgtgcgctt caccaaggtg gagatgaagg agaagatgct gcgcgccgcc 1141 cgcgagaagg gccgcgtgac cctgaagggc aagcccatcc gtctcaccgc cgacctgagc 1201 gccgagaccc tgcaggcccg ccgcgagtgg ggccccatct tcaacatcct gaaggagaag 1261 aacttccagc cccgcatcag ctaccccgcc aagctgagct tcatcagcga gggcgagatc 1321 aagtacttca tcgacaagca gatgctgcgc gacttcgtga ccacccgccc cgccctgaag 1381 gagctgctga aggaggccct gaacatggag cgcaacaacc gctaccagcc cctgcagaac 1441 cacgccaaga tgtaaagacc atcgagacta ggaagaaact gcatcaacta atgagcaaaa 1501 tcaggcgcgc ccatcataat gaccggcagc aacagccaca tcaccatcct gaccctgaac 1561 atcaacggcc tgaacagcgc catcaagcgc caccgcctgg ccagctggat caagagccag 1621 gaccccagcg tgtgctgcat ccaggagacc cacctgacct gccgcgacac ccaccgcctg 1681 aagatcaagg gctggcgcaa gatctaccag gccaacggca agcagaagaa ggccggcgtg 1741 gccatcctgg tgagcgacaa gaccgacttc aagcccacca agatcaagcg cgacaaggag 1801 ggccactaca tcatggtgaa gggcagcatc cagcaggagg agctgaccat cctgaacatc 1861 tacgccccca acaccggtgc cccccgcttc atcaagcagg tgctgagcga cctgcagcgc 1921 gacctggaca gccacaccct gatcatgggc gacttcaaca cccccctgag caccctggac 1981 cgcagcaccc gccagaaggt gaacaaggac acccaggagc tgaacagcgc cctgcaccag 2041 gccgacctga tcgacatcta ccgcaccctg caccccaaga gcaccgagta caccttcttc 2101 agcgcccccc accacaccta cagcaagatc gaccacatcg tgggcagcaa ggccctgctg 2161 agcaagtgca agcgcaccga gatcatcacc aactacctga gcgaccacag cgccatcaag 2221 ctggagctgc gcatcaagaa cctgacccag agccgcagca ccacctggaa gctgaacaac 2281 ctgctgctga acgactactg ggtgcacaac gagatgaagg ccgagatcaa gatgttcttc 2341 gaaaccaacg agaacaagga caccacctac cagaacctgt gggacgcctt caaggccgtg 2401 tgccgcggca agttcatcgc cctgaacgcc tacaagcgca agcaggagcg cagcaagatc 2461 gacaccctga ccagccagct gaaggagctg gagaagcagg agcagaccca cagcaaggcc 2521 agccgccgcc aggagatcac caagatccgc gccgagctga aggagatcga gacccagaag 2581 accctgcaga agatcaacga gtcgcgaagc tggttcttcg agcgcatcaa caagatcgac 2641 cgccccctgg cccgcctgat caagaagaag cgcgagaaga accagatcga caccatcaag 2701 aacgacaagg gcgacatcac caccgacccc accgagatcc agaccaccat ccgcgagtac 2761 tacaagcacc tgtacgccaa caagctggag aacctggagg agatggacac cttcctggac 2821 acctacaccc tgccccgcct gaaccaggag gaggtggaga gcctgaaccg ccccatcacc 2881 ggcagcgaga tcgtggccat catcaacagc ctgcccacca agaagagccc cggccccgac 2941 ggcttcaccg ccgagttcta ccagcgctac aaggaggagc tggtgccctt cctgctgaag 3001 ctgttccaaa gcatcgagaa ggagggcatc ctgcccaaca gcttctacga ggccagcatc 3061 atcctgatcc ccaagcccgg ccgcgacacc accaagaagg agaacttccg ccccatcagc 3121 ctgatgaaca tcgacgccaa gatcctgaac aagatcctgg ccaaccgcat ccagcagcac 3181 atcaagaagc tgatccacca cgaccaggtg ggcttcatcc ccgggatgca gggctggttc 3241 aacatccgca agagcatcaa cgtgatccag cacatcaacc gcgccaagga caagaaccac 3301 atgatcatca gcatcgacgc cgagaaggcc ttcgacaaga tccagcagcc cttcatgctg 3361 aagaccctga acaagctggg catcgacggc acctacttca agatcatccg cgccatctac 3421 gacaagccca ccgccaacat catcctgaac ggccagaagc tggaggcctt ccccctgaag 3481 accggcacgc gtcagggctg ccccctgagc cccctgctgt tcaacatcgt gctggaggtg 3541 ctggcccgcg ccatccgcca ggagaaggag atcaagggca tccagctggg caaggaggag 3601 gtgaagctga gcctgttcgc cgacgacatg atcgtgtacc tggagaaccc catcgtgagc 3661 gcccagaacc tgctgaagct gatcagcaac ttcagcaagg tgagcggcta caagatcaac 3721 gtgcagaaga gccaggcctt cctgtacacc aacaaccgcc agaccgagag ccagatcatg 3781 ggcgagctgc ccttcaccat cgctagcaag cgcatcaagt acctgggcat ccagctgacc 3841 cgcgacgtga aggacctgtt caaggagaac tacaagcccc tgctgaagga gatcaaggag 3901 gagaccaaca agtggaagaa catcccctgc agctgggtgg gccgcatcaa catcgtgaag 3961 atggccatcc tgcccaaggt gatctaccgc ttcaacgcca tccccatcaa gctgcccatg 4021 accttcttca ccgagctgga gaagaccacc ctgaagttca tctggaacca gaagcgcgcc 4081 cgcatcgcca agagcatcct gagccagaag aacaaggccg gcggcatcac cctgcccgac 4141 ttcaagctgt actacaaggc caccgtgacc aagaccgcct ggtactggta ccagaaccgc 4201 gatatcgacc agtggaaccg caccgagccc agcgagatca tgccccacat ctacaactac 4261 ctgatcttcg acaagcccga gaagaacaag cagtggggca aggacagcct gttcaacaag 4321 tggtgctggg agaactggct ggccatctgc cgcaagctga agctggaccc cttcctgacc 4381 ccctacacca agatcaacag ccgctggatc aaggacctga acgtgaagcc caagaccatc 4441 aagaccctgg aggagaacct gggcatcacc atccaggaca tcggcgtggg caaggacttc 4501 atgagcaaga cccccaaggc catggccacc aaggacaaga tcgacaagtg ggacctgatc 4561 aagctgaaga gcttctgcac cgccaaggag accaccatcc gcgtgaaccg ccagcccacc 4621 acctgggaga agatcttcgc cacctacagc agcgacaagg gcctgatcag ccgcatctac 4681 aacgagctga agcagatcta caagaagaag accaacaacc ccatcaagaa gtgggccaag 4741 gacatgaacc gccacttcag caaggaggac atctacgccg ccaagaagca tatgaagaag 4801 tgcagcagca gcctggccat ccgcgagatg cagatcaaga ccaccatgcg ctaccacctg 4861 acccccgtgc gcatggccat catcaagaag agcggcaaca accgctgctg gcgcggctgc 4921 ggcgagatcg gcaccctgct gcactgctgg tgggactgca agctggtgca gcccctgtgg 4981 aagagcgtgt ggcgcttcct gcgcgacctg gagctggaga tccccttcga ccccgccatc 5041 cccctgctgg gcatctaccc caacgagtac aagagctgct gctacaagga cacctgcacc 5101 cgcatgttca tcgccgccct gttcaccatc gccaagacct ggaaccagcc caagtgcccc 5161 accatgatcg attggatcaa gaagatgtgg cacatctaca ccatggagta ctacgccgcc 5221 atcaagaacg acgagttcat cagcttcgtg ggcacctgga tgaagctgga gaccatcatc 5281 ctgagcaagc tgagccagga gcagaagacc aagcaccgca tcttcagcct gatcggcggc 5341 aactgatgtc aggccaagct tccatcgata gacatgataa gatacattga tgagtttgga 5401 caaaccacaa caagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt 5461 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat 5521 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac 5581 aaatgtggta cctaggcgag accctgtctc acaaaataaa gtaagcccgg actgagtgcg 5641 gaaaggcggg cctggcgggt ctggtctccc catgcgggcc accagaggcc ctgcagcctt 5701 cagtcgcttg aaggggtaat ggcgcttcca ctcacaaaca tggcggacag agcgtgtgaa 5761 cgagatgaac agcccctcaa aaatatggcc gccgaggctg gacggccgtg ccccagcagc 5821 accgcctccg cgccccacgt gatctctcgc cgggcacagc gctgaccgcg gaggtccaac 5881 cggaagaatg tccggattgg acattcggaa gagggcccgc cttccctggg gaatctctgc 5941 gcacgcgcag aacgcttcga ccaatgaaaa cacaggaagc cgtccgcgca accgcgttgc 6001 gtcacttctg ccgcccctgt ttcaaggtat atagccgtag acggaacttc gcctttctct 6061 cggccttagc gccatttttt tgggtgagtg ttttttggtt cctgcgttgg gattccgtgt 6121 acaatccata gacatctgac ctcggcactt agcatcatca cagcaaacta actgtagcct 6181 ttctctcttt ccctgtagaa acctctgcac ctgaggccac catgttgagc aagggcgagg 6241 aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccatg 6301 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc 6361 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca 6421 tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt 6481 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga 6541 ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg 6601 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gacaatgggc tgggaggcct 6661 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga 6721 agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc 6781 ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg 6841 aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgt 6901 acggcatgga cgagctgtac aagggcagtg gagctactaa cttcagcctg ctgaagcagg 6961 ctggtgacgt cgaggagaat cctggcccca tgtctagact ggacaagagc aaagtcataa 7021 acggcgctct ggaattactc aatggagtcg gtatcgaagg cctgacgaca aggaaactcg 7081 ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc 7141 tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg 7201 aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc 7261 tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg 7321 aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac 7381 tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc 7441 atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga 7501 gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg 7561 aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg 7621 cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg 7681 atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtccggcg 7741 ctactaactt cagcctgctg aagcaggctg gtgacgtcga ggagaatcct ggtcccatga 7801 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 7861 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 7921 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 7981 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 8041 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 8101 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 8161 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 8221 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 8281 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 8341 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 8401 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 8461 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 8521 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 8581 agttcttctg attcgaaggc ctgtcgtgaa gcttggggat caattctcta gagctcgctg 8641 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 8701 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 8761 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 8821 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 8881 tgaggcggaa agaaccagct gggcctacta gctactcggg accccttacc gaaacatcgc 8941 cgcattctgc agaggagtcg agtgtatgta aacttctgac ccactgggaa tgtgatgaaa 9001 gaaataaaag ctgaaatgaa tcattctctc tactattatt ctgatatttc acattcttaa 9061 aataaagtgg tgatcctaac tgacctaaga cagggaattt ttactaggat taaatgtcag 9121 gaattgtgaa aaagtgagtt taaatgtatt tggctaaggt gtatgtaaac ttccgacttc 9181 aactgtatag ggatccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 9241 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 9301 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 9361 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 9421 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 9481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 9541 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 9601 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 9661 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 9721 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 9781 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 9841 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 9901 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 9961 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 10021 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 10081 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 10141 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 10201 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 10261 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 10321 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 10381 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 10441 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 10501 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 10561 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 10621 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 10681 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 10741 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 10801 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 10861 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 10921 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 10981 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 11041 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 11101 atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa 11161 aataccgcat caggaaattg taagcgttaa tattttgtta aaattggatc cctatacagt 11221 tgaagtcgga agtttacata cacttaagtt ggagtcatta aaactcgttt ttcaactact 11281 ccacaaattt cttgttaaca aacaatagtt ttggcaagtc agttaggaca tctactttgt 11341 gcatgacaca agtcattttt ccaacaattg tttacagaca gattatttca cttataattc 11401 actgtatcac aattccagtg ggtcagaagt ttacatacac taag //
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