| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-Puro , pLVXpuro |
| 质粒类型: | 哺乳动物细胞慢病毒表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8102 bp |
| 5' 测序引物及序列: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | 嘌呤霉素 |
| 备注: | 含有组成型CMV启动子的慢病毒载体 |
| 产品目录号: | 632164 |
| 稳定性: | / |
| 组成型: | -- |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLVX-Puro载体 pLVXpuro质粒基本信息
pLVX-Puro质粒图谱 pLVXpuro载体图谱和pLVX-Puro载体序列 pLVXpuro质粒序列多克隆位点信息
pLVX-Puro质粒 pLVXpuro载体简介
Description
pLVX-Puro is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to express your gene of interest in virtually any cell type, even primary cells. Expression of your gene is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the multiple cloning site (MCS), allowing constitutive, high level expression of your protein of interest.
pLVX-Puro contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (1), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (2). Finally, pLVX-Puro also contains a central polypurine tract (cPPT) element that increases nuclear importation of the viral genome during target cell infection, resulting in improved vector integration and more effi cient transduction (3). In addition to lentiviral elements, pLVX-Puro contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.
Use
pLVX-Puro constitutively expresses your gene of interest from PCMV IE when transduced into target cells. Before the vector can be transduced into cells, however, it must be transfected into 293T packaging cells with our Lenti-X™ HT Packaging System (Cat. Nos. 632160 and 632161). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (4).
pLVX-Puro质粒序列 pLVXpuro载体序列
LOCUS pLVX-Puro 8102 bp DNA circular SYN
DEFINITION pLVX-Puro
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..8102
/organism="pLVX-Puro"
/mol_type="other DNA"
misc_feature 454..634
/label="HIV-1_5_LTR"
misc_feature 454..634
/label="truncHIV-1_3_LTR"
misc_feature 745..789
/label="HIV-1_psi_pack"
CDS complement(1015..1476)
/label="ORF frame 3"
CDS 1181..2032
/label="ORF frame 2"
misc_feature 1303..1536
/label="RRE"
misc_feature 2031..2046
/label="cPPT"
promoter 2198..2702
/label="CMV_immearly_promoter"
misc_feature 2205..2492
/label="CAG_enhancer"
misc_feature 2659..2679
/label="CMV_fwd_primer"
promoter 2660..2729
/label="CMV_promoter"
misc_feature 2703..2722
/label="pCEP_fwd_primer"
misc_feature 2705..2729
/label="LNCX_primer"
misc_feature complement(2925..2948)
/label="MSCV_rev_primer"
CDS 3170..4009
/label="ORF frame 2"
misc_feature 3322..3341
/label="mPGK_F_primer"
gene 3410..4009
/label="puro (variant)"
/gene="puro (variant)"
misc_feature 4025..4600
/label="WPRE"
CDS 4113..4640
/label="ORF frame 3"
misc_feature 5271..5451
/label="HIV-1_5_LTR"
misc_feature 5271..5451
/label="truncHIV-1_3_LTR"
promoter complement(5576..5594)
/label="M13_reverse_primer"
misc_feature complement(5593..5615)
/label="M13_pUC_rev_primer"
promoter complement(5629..5658)
/label="lac_promoter"
gene complement(6741..7601)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(6741..7601)
/label="ORF frame 1"
promoter complement(7643..7671)
/label="AmpR_promoter"
terminator 7757..7888
/label="SV40_PA_terminator"
misc_feature 7845..7864
/label="EBV_rev_primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag
2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagat
2881 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
2941 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
3001 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
3061 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
3121 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
3181 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
3241 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
3301 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
3361 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta
3421 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
3481 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
3541 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
3601 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
3661 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
3721 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
3781 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
3841 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
3901 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
3961 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga
4021 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
4081 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
4141 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
4201 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
4261 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
4321 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
4381 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
4441 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
4501 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
4561 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
4621 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
4681 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
4741 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
4801 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
4861 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
4921 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
4981 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
5041 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
5101 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
5161 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
5221 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
5281 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
5341 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
5401 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
5461 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
5521 ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg
5581 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
5641 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
5701 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
5761 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
5821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
5881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
5941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
6001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
6061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
6121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
6181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
6241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
6301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
6361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
6421 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
6481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
6541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
6601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
6661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
6721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
6781 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
6841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
6901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
6961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
7021 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
7081 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
7141 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
7201 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
7261 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
7321 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
7381 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
7441 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
7501 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
7561 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
7621 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
7681 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
7741 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
7801 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
7861 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
7921 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
7981 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
8041 tacaaactta gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta
8101 gt
//
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