| 出品公司: | Clontech |
|---|---|
| 载体名称: | pCMV-HA-N |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 3782 bp |
| 5' 测序引物及序列: | pCMV-F: 5'd[GATCCGGTACTAGAGGAACTGAAAAAC]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | HA |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-HA-N 3782 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Vector for expressing an N-terminally HA-tagged protein in mammalian
cells. Identical to pCMV-HA.
ACCESSION .
VERSION .
KEYWORDS pCMV-HA-N
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3782)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Saturday, December 21, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3782
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 27..330
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 672..768
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 829..831
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 832..858
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
misc_feature 863..917
/label=MCS
/note="MCS"
/note="multiple cloning site"
polyA_signal 921..1055
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(1166..1182)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1190..1206
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1214..1244)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(1568..2156)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2327..3187)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3188..3292)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
841 gatgttccag attacgctct tatggccatg gaggcccgaa ttcggtcgac cgagatctct
901 cgaggtaccg cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa
961 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
1021 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
1081 tgtttcaggt tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgatctgca
1141 ggcatgctag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
1201 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
1261 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
1321 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
1381 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
1441 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
1501 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
1561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
1621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
1681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
1741 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
1801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
1861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
1921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
1981 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
2041 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
2101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
2161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
2221 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
2281 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
2341 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
2401 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
2461 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
2521 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
2581 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
2641 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
2701 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
2761 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
2821 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
2881 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
2941 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
3001 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
3061 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
3121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
3181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
3241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
3301 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
3361 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
3421 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
3481 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
3541 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
3601 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
3661 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
3721 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
3781 gt
//
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