| 出品公司: | Clontech |
|---|---|
| 载体名称: | pCMV-Myc-C |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 启动子: | cmv |
| 载体大小: | 3772 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | M13 rev |
| 载体标签: | Myc |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-Myc-C 3772 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Mammalian vector for CMV promoter-driven expression of an ORF with a
C-terminal Myc tag.
ACCESSION .
VERSION .
KEYWORDS pCMV-Myc-C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3772)
AUTHORS transOMIC, Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Saturday, December 21, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3772
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 27..330
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 672..768
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
misc_feature 832..863
/note="MCS"
/note="multiple cloning site"
CDS 867..896
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
polyA_signal 911..1045
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(1156..1172)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(1558..2146)
/direction=LEFT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2317..3177)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3178..3282)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcggt
841 cgaccgagat ctctcgaggt accggcgagc agaagctgat ctcagaggag gacctgtaag
901 cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
961 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
1021 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
1081 tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgatctgca ggcatgctag
1141 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
1201 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
1261 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
1321 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
1381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
1441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
1501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
1561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
1621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
1681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
1741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
1801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
1861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
1921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
1981 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
2041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
2101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
2161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
2221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
2281 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
2341 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
2401 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
2461 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
2521 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
2581 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
2641 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
2701 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
2761 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
2821 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
2881 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
2941 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
3001 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
3061 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
3121 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
3181 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
3241 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
3301 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
3361 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
3421 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
3481 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
3541 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
3601 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
3661 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
3721 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gt
//
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