出品公司: | Clontech |
---|---|
载体名称: | pCMV-Myc-C |
质粒类型: | 哺乳动物细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 多克隆位点,限制性内切酶 |
启动子: | cmv |
载体大小: | 3772 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | M13 rev |
载体标签: | Myc |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | -- |
产品目录号: | -- |
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pCMV-Myc-C载体质粒基本信息
pCMV-Myc-C质粒图谱载体图谱和pCMV-Myc-C载体序列质粒序列多克隆位点信息
pCMV-Myc-C质粒载体简介
pCMV-Myc-C质粒序列载体序列
LOCUS pCMV-Myc-C 3772 bp ds-DNA circular SYN 19-OCT-2017 DEFINITION Mammalian vector for CMV promoter-driven expression of an ORF with a C-terminal Myc tag. ACCESSION . VERSION . KEYWORDS pCMV-Myc-C SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3772) AUTHORS transOMIC, Clontech (TaKaRa) TITLE Direct Submission JOURNAL Exported Saturday, December 21, 2019 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3772 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 27..330 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 331..534 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" intron 672..768 /note="SV40 intron" /note="modified SV40 intron with splice donor and acceptor sites" misc_feature 832..863 /note="MCS" /note="multiple cloning site" CDS 867..896 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /note="Myc" /translation="EQKLISEEDL" polyA_signal 911..1045 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" primer_bind complement(1156..1172) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1558..2146) /direction=LEFT /note="ori" /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" CDS complement(2317..3177) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3178..3282) /gene="bla" /note="AmpR promoter" ORIGIN 1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc 61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta 541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac 601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag 661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg 721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa 781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcggt 841 cgaccgagat ctctcgaggt accggcgagc agaagctgat ctcagaggag gacctgtaag 901 cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag 961 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac 1021 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt 1081 tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgatctgca ggcatgctag 1141 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 1201 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 1261 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 1321 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 1381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 1441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 1501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 1561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 1621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 1681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 1741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 1801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 1861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 1921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 1981 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 2041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 2101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 2161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 2221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 2281 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 2341 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 2401 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 2461 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 2521 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 2581 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 2641 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 2701 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 2761 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 2821 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 2881 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 2941 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 3001 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 3061 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 3121 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 3181 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 3241 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 3301 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 3361 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 3421 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 3481 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 3541 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 3601 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 3661 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 3721 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gt //
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