| 出品公司: | Clontech |
|---|---|
| 载体名称: | pCMV-Myc-N |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 3794 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | M13 rev |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
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载体基本信息
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载体简介
载体序列
LOCUS pCMV-Myc-N 3794 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Mammalian vector for CMV promoter-driven expression of an ORF with
an N-terminal Myc tag.
ACCESSION .
VERSION .
KEYWORDS pCMV-Myc-N
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3794)
AUTHORS transOMIC, Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Saturday, December 21, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3794
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 27..330
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 672..768
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 838..840
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 841..870
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
misc_feature 875..929
/note="MCS"
/note="multiple cloning site"
polyA_signal 933..1067
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(1178..1194)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(1580..2168)
/direction=LEFT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2339..3199)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3200..3304)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggcatcaatg
841 gagcagaagc tgatctcaga ggaggacctg cttatggcca tggaggcccg aattcggtcg
901 accgagatct ctcgaggtac cgcggccgcg gggatccaga catgataaga tacattgatg
961 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
1021 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
1081 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcg gatcctctag
1141 agtcgatctg caggcatgct agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
1201 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
1261 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
1321 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
1381 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
1441 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
1501 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
1561 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
1621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
1681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
1741 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
1801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
1861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
1921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
1981 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
2041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
2101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
2161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
2221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
2281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
2341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
2401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
2461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
2521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
2581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
2641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
2701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
2761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
2821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
2881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
2941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
3001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
3061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
3121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
3181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
3241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
3301 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
3361 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
3421 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
3481 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
3541 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
3601 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
3661 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
3721 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
3781 aaacgacggc cagt
//
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