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p3xFLAG-Myc-CMV-24载体质粒基本信息
p3xFLAG-Myc-CMV-24质粒图谱载体图谱和p3xFLAG-Myc-CMV-24载体序列质粒序列多克隆位点信息
p3xFLAG-Myc-CMV-24质粒载体简介
p3xFLAG-Myc-CMV-24质粒序列载体序列
LOCUS p3xFLAG-Myc-CMV-24 4752 bp ds-DNA circular SYN 23-OCT-2018 DEFINITION Vector for expression of 3xFLAG- and Myc-tagged proteins in mammalian cells. ACCESSION . VERSION . KEYWORDS p3xFLAG-Myc-CMV-24 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4752) AUTHORS MilliporeSigma TITLE Direct Submission JOURNAL Exported Saturday, December 21, 2019 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4752 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 318..697 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 698..901 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 928..930 /codon_start=1 /product="start codon" /note="ATG" /translation="M" CDS 931..996 /codon_start=1 /product="three tandem FLAG epitope tags, followed by an enterokinase cleavage site" /note="3xFLAG" /translation="DYKDHDGDYKDHDIDYKDDDDK" misc_feature 994..1050 /note="MCS" /note="multiple cloning site" CDS 1057..1086 /codon_start=1 /product="Myc (human c-Myc oncogene) epitope tag" /note="Myc" /translation="EQKLISEEDL" polyA_signal 1093..1715 /note="hGH poly(A) signal" /note="human growth hormone polyadenylation signal" rep_origin 1924..2059 /note="SV40 ori" /note="SV40 origin of replication" rep_origin complement(2546..3134) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3305..4165) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4166..4270) /gene="bla" /note="AmpR promoter" rep_origin 4297..4752 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa 181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa 961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttcatcgata 1021 gatctgatat cggtaccagt cgactctaga ggatccgaac aaaaactcat ctcagaagag 1081 gatctgtgac ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa 1141 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct 1201 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa 1261 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg 1321 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag 1381 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt 1441 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg 1501 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc 1561 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg 1621 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca 1681 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ttggctgtgg 1741 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 1801 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 1861 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 1921 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 1981 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 2041 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag 2101 aaagttaatt ccctatagtg agtcgtatta aattcgtaat catgtcatag ctgtttcctg 2161 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 2221 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 2281 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 2341 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 2401 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 2461 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 2521 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 2581 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 2641 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 2701 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 2761 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 2821 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 2881 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 2941 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 3001 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 3061 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 3121 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 3181 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 3241 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 3301 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 3361 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 3421 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 3481 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 3541 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 3601 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 3661 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 3721 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 3781 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 3841 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 3901 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 3961 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 4021 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 4081 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 4141 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 4201 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 4261 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa 4321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 4381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 4441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 4501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 4561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 4621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 4681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 4741 cgcttacaat tt //