| 出品公司: | Clontech |
|---|---|
| 载体名称: | pTRE2hyg |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 5337 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTRE2hyg 5337 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Expression vector with a Tet-responsive promoter and a hygromycin
resistance marker.
ACCESSION .
VERSION .
KEYWORDS pTRE2hyg
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5337)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Saturday, December 21, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5337
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
protein_bind 15..285
/bound_moiety="tetracycline repressor TetR"
/note="tetracycline response element"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 318..356
/note="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 471..532
/note="MCS"
/note="multiple cloning site"
intron 554..1126
/note="beta-globin intron"
/note="intron from rabbit beta-globin gene"
polyA_site 1323..1378
/note="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal"
rep_origin complement(1947..2535)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2706..3566)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3567..3671)
/gene="bla"
/note="AmpR promoter"
polyA_signal 3777..3858
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(3975..5000)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
promoter complement(5009..1)
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 5023..5158
/note="SV40 ori"
/note="SV40 origin of replication"
ORIGIN
1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
301 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
361 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
421 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta
481 gtcagctgac gcgtgctagc gcggccgcat cgataagctt gtcgacgata tctctagagc
541 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
601 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
661 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
721 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
781 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
841 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
901 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
961 gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc
1021 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
1081 gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc tgggcaacgt
1141 gctggttgtt gtgctgtctc atcattttgg caaagaattc actcctcagg tgcaggctgc
1201 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
1261 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
1321 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
1381 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
1441 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
1501 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
1561 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
1621 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
1681 tgtccctctt ctcttatgga gatccctcga ctgcattaat gaatcggcca acgcgcgggg
1741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
1801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
1861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
1921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
1981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
2041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
2101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
2161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
2221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
2281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
2341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
2401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
2461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
2521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
2581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
2641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
2701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
2761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
2821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
2881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
2941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
3001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
3061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
3121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
3181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
3241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
3301 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
3361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
3421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
3481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
3541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
3601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
3661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
3721 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca ctcgaggcag
3781 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
3841 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
3901 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
3961 gatcctctag aactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta
4021 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
4081 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa
4141 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg
4201 agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag
4261 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg
4321 tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg
4381 tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag
4441 tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg
4501 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc
4561 catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta
4621 agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt
4681 tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc
4741 aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca
4801 aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg
4861 acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc
4921 tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg
4981 gtgagttcag gctttttcat ggaagctttt tgcaaaagcc taggcctcca aaaaagcctc
5041 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
5101 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
5161 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
5221 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
5281 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacaca
//
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