出品公司: | Clontech |
---|---|
载体名称: | pTRE2hyg |
质粒类型: | 哺乳动物细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | -- |
载体大小: | 5337 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | -- |
宿主细胞(系): | -- |
备注: | -- |
买家导航
pTRE2hyg载体质粒基本信息
pTRE2hyg质粒图谱载体图谱和pTRE2hyg载体序列质粒序列多克隆位点信息
pTRE2hyg质粒载体简介
pTRE2hyg质粒序列载体序列
LOCUS pTRE2hyg 5337 bp ds-DNA circular SYN 19-OCT-2017 DEFINITION Expression vector with a Tet-responsive promoter and a hygromycin resistance marker. ACCESSION . VERSION . KEYWORDS pTRE2hyg SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5337) AUTHORS Clontech (TaKaRa) TITLE Direct Submission JOURNAL Exported Saturday, December 21, 2019 from SnapGene Viewer 3.1.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5337 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" protein_bind 15..285 /bound_moiety="tetracycline repressor TetR" /note="tetracycline response element" /note="contains seven copies of the tetracycline operator tetO" promoter 318..356 /note="minimal CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 471..532 /note="MCS" /note="multiple cloning site" intron 554..1126 /note="beta-globin intron" /note="intron from rabbit beta-globin gene" polyA_site 1323..1378 /note="beta-globin poly(A) signal" /note="rabbit beta-globin polyadenylation signal" rep_origin complement(1947..2535) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2706..3566) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3567..3671) /gene="bla" /note="AmpR promoter" polyA_signal 3777..3858 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" CDS complement(3975..5000) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" promoter complement(5009..1) /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 5023..5158 /note="SV40 ori" /note="SV40 origin of replication" ORIGIN 1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc 61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa 121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc 181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag 241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag 301 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt 361 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac 421 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta 481 gtcagctgac gcgtgctagc gcggccgcat cgataagctt gtcgacgata tctctagagc 541 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa 601 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc 661 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca 721 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt 781 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct 841 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta 901 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg 961 gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc 1021 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc 1081 gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc tgggcaacgt 1141 gctggttgtt gtgctgtctc atcattttgg caaagaattc actcctcagg tgcaggctgc 1201 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct 1261 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 1321 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 1381 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag 1441 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca 1501 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg 1561 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt 1621 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc 1681 tgtccctctt ctcttatgga gatccctcga ctgcattaat gaatcggcca acgcgcgggg 1741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 1801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 1861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 1921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 1981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 2041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 2101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 2161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 2221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 2281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 2341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 2401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 2461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 2521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 2581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 2641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 2701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 2761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 2821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 2881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 2941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 3001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 3061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 3121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 3181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 3241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 3301 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 3361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 3421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 3481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 3541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 3601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 3661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 3721 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca ctcgaggcag 3781 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 3841 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 3901 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat 3961 gatcctctag aactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta 4021 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt 4081 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa 4141 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg 4201 agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag 4261 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg 4321 tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg 4381 tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag 4441 tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg 4501 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc 4561 catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta 4621 agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt 4681 tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc 4741 aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca 4801 aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg 4861 acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc 4921 tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg 4981 gtgagttcag gctttttcat ggaagctttt tgcaaaagcc taggcctcca aaaaagcctc 5041 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa 5101 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg 5161 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc 5221 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc 5281 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacaca //
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