| 质粒名称: | pLI50 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 13573 |
| 存储实验室: | Chia Lee |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5505 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | S、 大肠杆菌穿梭载体 |
| 筛选标记: | 37℃下金黄色葡萄球菌中的氯霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLI50 was a gift from Chia Lee (Addgene plasmid # 13573 ; http://n2t.net/addgene:13573 ; RRID:Addgene_13573) |
| 参考文献部分: | Construction of single-copy integration vectors for Staphylococcus aureus. Lee CY, Buranen SL, Ye ZH. Gene. 1991 Jul 15. 103(1):101-5. 10.1016/0378-1119(91)90399-V PubMed 1652539 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLI50 5505 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLI50
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5505)
AUTHORS Lee CY, Buranen SL, Ye ZH
TITLE Construction of single-copy integration vectors for Staphylococcus
aureus.
JOURNAL Gene. 1991 Jul 15. 103(1):101-5.
PUBMED 1652539
REFERENCE 2 (bases 1 to 5505)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5505
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..57
/label=MCS
/note="pUC18/19 multiple cloning site"
CDS 1737..2741
/codon_start=1
/gene="repB"
/product="RepB replication protein"
/label=repB
/note="from Enterococcus faecalis plasmid pAM-alpha-1"
/db_xref="GI:22652809"
/protein_id="AAN03827.1"
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
primer_bind complement(3247..3269)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3355..3495
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 3410..3429
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(3510..3527)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3681..4269)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3761..3780)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4440..5300)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5063..5082
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5301..5405)
/gene="bla"
/label=AmpR promoter
primer_bind 5473..5491
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttctg
61 taggttttta ggcataaaac tatatgattt acccctaaat ctttaaaatg ccccttaaaa
121 ttcaaaataa aggcatttaa aatttaaata tttcttgtga taaagtttgt taaaaaggag
181 tggttttatg actgttatgt ggttatcgat tataggtatg tggttttgta ttggaatggc
241 attttttgct atcaaggtta ttaaaaataa aaattagacc acgcatttat gccgagaaaa
301 tttattgtgc gttgagaaga acccttaact aaacttgcag acgaatgtcg gcatagcgtg
361 agctattaag ccgaccattc gacaagtttt gggattgtta agggttccga ggctcaacgt
421 caataaagca attggaataa agaagcgaaa aaggagaagt cggttcagaa aaagaaggat
481 atggatctgg agctgtaata taaaaacctt cttcaactaa cggggcaggt tagtgacatt
541 agaaaaccga ctgtaaaaag tacagtcggc attatctcat attataaaag ccagtcatta
601 ggcctatctg acaattcctg aatagagttc ataaacaatc ctgcatgata accatcacaa
661 acagaatgat gtacctgtaa agatagcggt aaatatattg aattaccttt attaatgaat
721 tttcctgctg taataatggg tagaaggtaa ttactattat tattgatatt taagttaaac
781 ccagtaaatg aagtccatgg aataatagaa agagaaaaag cattttcagg tataggtgtt
841 ttgggaaaca atttccccga accattatat ttctctacat cagaaaggta taaatcataa
901 aactctttga agtcattctt tacaggagtc caaataccag agaatgtttt agatacacca
961 tcaaaaattg tataaagtgg ctctaactta tcccaataac ctaactctcc gtcgctattg
1021 taaccagttc taaaagctgt atttgagttt atcacccttg tcactaagaa aataaatgca
1081 gggtaaaatt tatatccttc ttgttttatg tttcggtata aaacactaat atcaatttct
1141 gtggttatac taaaagtcgt ttgttggttc aaataatgat taaatatctc ttttctcttc
1201 caattgtcta aatcaatttt attaaagttc atttgatatg cctcctaaat ttttatctaa
1261 agtgaattta ggaggcttac ttgtctgctt tcttcattag aatcaatcct ttttttaaaa
1321 gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat ccaattttcg
1381 tttgttgaat ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat
1441 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaaaga
1501 ccacattaaa aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc
1561 cttttctttc ttatcttgat acatatagaa ataacgtcat ttttatttta gttgctgaaa
1621 ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt
1681 gcacagaaaa accccatctg ttaaagttat aagtgactaa acaaataact aaatagatgg
1741 gggtttcttt taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg
1801 ttttagtgga caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta
1861 atgttgatta ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag
1921 attgtgctga aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc
1981 gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac
2041 atggcattca gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt
2101 ggttgtttct cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt
2161 tgtcagatat ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatc
2221 ttgttggttt tatgcgtgca acggaagtga caataaataa taaagataat tcttataatc
2281 agcacatgca tgtattggta tgtgtggaac caacttattt taagaataca gaaaactacg
2341 tgaatcaaaa acaatggatt caattttgga aaaaggcaat gaaattagac tatgatccaa
2401 atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa atcggatata caatcggcaa
2461 ttgacgaaac tgcaaaatat cctgtaaagg atacggattt tatgaccgat gatgaagaaa
2521 agaatttgaa acgtttgtct gatttggagg aaggtttaca ccgtaaaagg ttaatctcct
2581 atggtggttt gttaaaagaa atacataaaa aattaaacct tgatgacaca gaagaaggcg
2641 atttgattca tacagatgat gacgaaaaag ccgatgaaga tggattttct attattgcaa
2701 tgtggaattg ggaacggaaa aattatttta ttaaagagta gttcaacaaa cgggccagtt
2761 tgttgaagat tagatgctat aattgttatt aaaaggattg aaggatgctt aggaagacga
2821 gttattaata gctgaataag aacggtgctc tccaaatatt cttatttaga aaagcaaatc
2881 taaaattatc tgaaaaggga atgagaatag tgaatggacc aataataatg actagagaag
2941 aaagaatgaa gattgttcat gaaattaagg aacgaatatt ggataaatat ggggatgatg
3001 ttaaggctat tggtgtttat ggctctcttg gtcgtcagac tgatgggccc tattcggata
3061 ttgagatgat gtgtgtcatg tcaacagagg aagcagagtt cagccatgaa tggacaaccg
3121 gaaagagcga aaatgcctca catttgtgcc acctaaaaag gagcgattta catatgagtt
3181 atgcagtttg tagaatgcaa aaagtgaaat cagctgcctc gcgcgtttcg gtgatgacgg
3241 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
3301 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
3361 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
3421 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
3481 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
3541 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
3601 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
3661 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
3721 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
3781 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
3841 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
3901 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
3961 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
4021 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
4081 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
4141 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
4201 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
4261 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
4321 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
4381 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
4441 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
4501 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
4561 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
4621 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
4681 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
4741 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
4801 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
4861 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
4921 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
4981 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
5041 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
5101 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
5161 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
5221 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
5281 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
5341 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
5401 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
5461 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaa
//
