| 质粒名称: | pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 60910 |
| 存储实验室: | Ron Vale |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体抗性: | 氨苄霉素 |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCas9 |
| 启动子 | dSV40 |
| 标签/融合蛋白: | NLS（N端） HA（C端） 2x NLS（C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | 未知 |
| 3' 克隆位点: | BamHI (未破坏) |
| 5' 测序引物 | SV40pro-F2 |
| 3' 测序引物 | mTagBFP-R |
|
文献引用方法 |
|
| 材料和方法部分: | pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE was a gift from Ron Vale (Addgene plasmid # 60910) |
| 参考文献部分: | A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. 10.1016/j.cell.2014.09.039 PubMed 25307933 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE 14911 bp DNA SYN
DEFINITION pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..14911
/organism="pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE"
/mol_type="other DNA"
misc_feature complement(103..120)
/label="Sp6_primer"
gene 358..621
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(410..429)
/label="pBRrevBam_primer"
misc_feature 763..785
/label="pGEX_3_primer"
promoter 944..972
/label="AmpR_promoter"
gene 1014..1874
/label="Ampicillin"
/gene="Ampicillin"
CDS 1014..1874
/label="ORF frame 3"
rep_origin 2029..2648
/label="pBR322_origin"
misc_feature complement(2874..2894)
/label="pBABE_3_primer"
misc_feature complement(2880..3333)
/label="SV40_enhancer"
promoter 2892..3160
/label="SV40_promoter"
rep_origin 3059..3136
/label="SV40_origin"
misc_feature 3121..3140
/label="SV40pro_F_primer"
misc_feature 4341..4356
/label="SV40_int"
misc_feature 4362..4409
/label="SV40_3_splice"
CDS 4486..4959
/label="ORF frame 1"
misc_feature 4537..4557
/label="SV40 NLS"
misc_feature 4543..4557
/label="NLS"
terminator 4985..5116
/label="SV40_PA_terminator"
misc_feature 5073..5092
/label="EBV_rev_primer"
misc_feature 5738..5918
/label="HIV-1_5_LTR"
misc_feature 5738..5918
/label="truncHIV-1_3_LTR"
misc_feature 6029..6073
/label="HIV-1_psi_pack"
CDS 6460..7557
/label="ORF frame 1"
misc_feature 6582..6815
/label="RRE"
misc_feature 7333..7348
/label="cPPT"
promoter 7531..7710
/label="SV40_promoter"
rep_origin 7578..7655
/label="SV40_origin"
misc_feature 7640..7659
/label="SV40pro_F_primer"
CDS 7732..14640
/label="ORF frame 1"
misc_feature 7735..7758
/label="SV40 NLS"
misc_feature 7735..7755
/label="SV40 NLS"
misc_feature 7741..7755
/label="NLS"
CDS complement(7774..12114)
/label="ORF frame 2"
misc_feature 11917..11943
/label="HA"
CDS complement(13720..14445)
/label="ORF frame 2"
misc_feature 14738..14759
/label="U3PPT"
misc_feature 14738..14753
/label="cPPT"
misc_feature 14755..14807
/label="delta_U3"
misc_feature 14808..77
/label="HIV-1_5_LTR"
misc_feature 14808..77
/label="truncHIV-1_3_LTR"
ORIGIN
1 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
61 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
121 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
181 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
241 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
301 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
361 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
421 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
481 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
541 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
601 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct agctctgatg
661 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
721 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
781 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
841 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
1021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
1081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
1141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
1201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
1261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
1321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
1381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
1441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
1501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
1561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
1621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
1681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
1741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
1801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
1861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
1921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
1981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
2041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
2101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
2161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
2221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
2281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
2341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
2401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
2461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
2521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
2581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
2641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
2701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
2761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
2821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
2881 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
2941 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
3001 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
3061 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
3121 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
3181 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
3241 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
3301 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
3361 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
3421 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
3481 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
3541 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
3601 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
3661 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
3721 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
3781 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
3841 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
3901 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
3961 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
4021 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
4081 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
4141 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
4201 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
4261 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
4321 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
4381 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
4441 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
4501 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
4561 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
4621 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
4681 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
4741 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
4801 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
4861 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
4921 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
4981 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
5041 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
5101 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
5161 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
5221 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
5281 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
5341 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
5401 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
5461 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
5521 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
5581 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
5641 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
5701 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
5761 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
5821 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
5881 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
5941 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
6001 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
6061 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
6121 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
6181 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
6241 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
6301 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
6361 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
6421 cagcaagcgg ccggtgatct tcagacctgg acgatatata tgagggacaa ttggagaagt
6481 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
6541 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
6601 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
6661 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
6721 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
6781 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
6841 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
6901 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
6961 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
7021 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
7081 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
7141 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
7201 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
7261 agagacagat ccattcgatt agtgaacgga tctcgacggt ATcgccaaat ggcagtattc
7321 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
7381 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
7441 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
7501 gaattcaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
7561 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
7621 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
7681 tccagaagta gtgaggaggc ttttttggag gcctaggcta cgcgcGCCAC CATGCCCAAG
7741 AAGAAGCGCA AGGTGGGacg cgtctgcagg atatcaagct tgcggtaccg cgggcccggg
7801 atcGCCACCA TGGACAAGAA GTACAGCATC GGCCTGGCCA TCGGCACCAA CTCTGTGGGC
7861 TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA AATTCAAGGT GCTGGGCAAC
7921 ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGCGCCC TGCTGTTCGA CAGCGGAGAA
7981 ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA GATACACCAG ACGGAAGAAC
8041 CGGATCTGCT ATCTGCAAGA GATCTTCAGC AACGAGATGG CCAAGGTGGA CGACAGCTTC
8101 TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA AGAAGCACGA GCGGCACCCC
8161 ATCTTCGGCA ACATCGTGGA CGAGGTGGCC TACCACGAGA AGTACCCCAC CATCTACCAC
8221 CTGAGAAAGA AACTGGTGGA CAGCACCGAC AAGGCCGACC TGCGGCTGAT CTATCTGGCC
8281 CTGGCCCACA TGATCAAGTT CCGGGGCCAC TTCCTGATCG AGGGCGACCT GAACCCCGAC
8341 AACAGCGACG TGGACAAGCT GTTCATCCAG CTGGTGCAGA CCTACAACCA GCTGTTCGAG
8401 GAAAACCCCA TCAACGCCAG CGGCGTGGAC GCCAAGGCCA TCCTGTCTGC CAGACTGAGC
8461 AAGAGCAGAC GGCTGGAAAA TCTGATCGCC CAGCTGCCCG GCGAGAAGAA GAATGGCCTG
8521 TTCGGCAACC TGATTGCCCT GAGCCTGGGC CTGACCCCCA ACTTCAAGAG CAACTTCGAC
8581 CTGGCCGAGG ATGCCAAACT GCAGCTGAGC AAGGACACCT ACGACGACGA CCTGGACAAC
8641 CTGCTGGCCC AGATCGGCGA CCAGTACGCC GACCTGTTTC TGGCCGCCAA GAACCTGTCC
8701 GACGCCATCC TGCTGAGCGA CATCCTGAGA GTGAACACCG AGATCACCAA GGCCCCCCTG
8761 AGCGCCTCTA TGATCAAGAG ATACGACGAG CACCACCAGG ACCTGACCCT GCTGAAAGCT
8821 CTCGTGCGGC AGCAGCTGCC TGAGAAGTAC AAAGAGATTT TCTTCGACCA GAGCAAGAAC
8881 GGCTACGCCG GCTACATCGA TGGCGGAGCC AGCCAGGAAG AGTTCTACAA GTTCATCAAG
8941 CCCATCCTGG AAAAGATGGA CGGCACCGAG GAACTGCTCG TGAAGCTGAA CAGAGAGGAC
9001 CTGCTGCGGA AGCAGCGGAC CTTCGACAAC GGCAGCATCC CCCACCAGAT CCACCTGGGA
9061 GAGCTGCACG CCATTCTGCG GCGGCAGGAA GATTTTTACC CATTCCTGAA GGACAACCGG
9121 GAAAAGATCG AGAAGATCCT GACCTTCCGC ATCCCCTACT ACGTGGGCCC TCTGGCCAGG
9181 GGAAACAGCA GATTCGCCTG GATGACCAGA AAGAGCGAGG AAACCATCAC CCCCTGGAAC
9241 TTCGAGGAAG TGGTGGACAA GGGCGCCAGC GCCCAGAGCT TCATCGAGCG GATGACCAAC
9301 TTCGATAAGA ACCTGCCCAA CGAGAAGGTG CTGCCCAAGC ACAGCCTGCT GTACGAGTAC
9361 TTCACCGTGT ACAACGAGCT GACCAAAGTG AAATACGTGA CCGAGGGAAT GAGAAAGCCC
9421 GCCTTCCTGA GCGGCGAGCA GAAAAAAGCC ATCGTGGACC TGCTGTTCAA GACCAACCGG
9481 AAAGTGACCG TGAAGCAGCT GAAAGAGGAC TACTTCAAGA AAATCGAGTG CTTCGACTCC
9541 GTGGAAATCT CCGGCGTGGA AGATCGGTTC AACGCCTCCC TGGGCACATA CCACGATCTG
9601 CTGAAAATTA TCAAGGACAA GGACTTCCTG GACAATGAGG AAAACGAGGA CATTCTGGAA
9661 GATATCGTGC TGACCCTGAC ACTGTTTGAG GACAGAGAGA TGATCGAGGA ACGGCTGAAA
9721 ACCTATGCCC ACCTGTTCGA CGACAAAGTG ATGAAGCAGC TGAAGCGGCG GAGATACACC
9781 GGCTGGGGCA GGCTGAGCCG GAAGCTGATC AACGGCATCC GGGACAAGCA GTCCGGCAAG
9841 ACAATCCTGG ATTTCCTGAA GTCCGACGGC TTCGCCAACA GAAACTTCAT GCAGCTGATC
9901 CACGACGACA GCCTGACCTT TAAAGAGGAC ATCCAGAAAG CCCAGGTGTC CGGCCAGGGC
9961 GATAGCCTGC ACGAGCACAT TGCCAATCTG GCCGGCAGCC CCGCCATTAA GAAGGGCATC
10021 CTGCAGACAG TGAAGGTGGT GGACGAGCTC GTGAAAGTGA TGGGCCGGCA CAAGCCCGAG
10081 AACATCGTGA TCGAAATGGC CAGAGAGAAC CAGACCACCC AGAAGGGACA GAAGAACAGC
10141 CGCGAGAGAA TGAAGCGGAT CGAAGAGGGC ATCAAAGAGC TGGGCAGCCA GATCCTGAAA
10201 GAACACCCCG TGGAAAACAC CCAGCTGCAG AACGAGAAGC TGTACCTGTA CTACCTGCAG
10261 AATGGGCGGG ATATGTACGT GGACCAGGAA CTGGACATCA ACCGGCTGTC CGACTACGAT
10321 GTGGACGCTA TCGTGCCTCA GAGCTTTCTG AAGGACGACT CCATCGATAA CAAAGTGCTG
10381 ACTCGGAGCG ACAAGAACCG GGGCAAGAGC GACAACGTGC CCTCCGAAGA GGTCGTGAAG
10441 AAGATGAAGA ACTACTGGCG CCAGCTGCTG AATGCCAAGC TGATTACCCA GAGGAAGTTC
10501 GACAATCTGA CCAAGGCCGA GAGAGGCGGC CTGAGCGAAC TGGATAAGGC CGGCTTCATC
10561 AAGAGACAGC TGGTGGAAAC CCGGCAGATC ACAAAGCACG TGGCACAGAT CCTGGACTCC
10621 CGGATGAACA CTAAGTACGA CGAGAACGAC AAACTGATCC GGGAAGTGAA AGTGATCACC
10681 CTGAAGTCCA AGCTGGTGTC CGATTTCCGG AAGGATTTCC AGTTTTACAA AGTGCGCGAG
10741 ATCAACAACT ACCACCACGC CCACGACGCC TACCTGAACG CCGTCGTGGG AACCGCCCTG
10801 ATCAAAAAGT ACCCTAAGCT GGAAAGCGAG TTCGTGTACG GCGACTACAA GGTGTACGAC
10861 GTGCGGAAGA TGATCGCCAA GAGCGAGCAG GAAATCGGCA AGGCTACCGC CAAGTACTTC
10921 TTCTACAGCA ACATCATGAA CTTTTTCAAG ACCGAGATTA CCCTGGCCAA CGGCGAGATC
10981 CGGAAGCGGC CTCTGATCGA GACAAACGGC GAAACAGGCG AGATCGTGTG GGATAAGGGC
11041 CGGGACTTTG CCACCGTGCG GAAAGTGCTG TCTATGCCCC AAGTGAATAT CGTGAAAAAG
11101 ACCGAGGTGC AGACAGGCGG CTTCAGCAAA GAGTCTATCC TGCCCAAGAG GAACAGCGAC
11161 AAGCTGATCG CCAGAAAGAA GGACTGGGAC CCTAAGAAGT ACGGCGGCTT CGACAGCCCC
11221 ACCGTGGCCT ATTCTGTGCT GGTGGTGGCC AAAGTGGAAA AGGGCAAGTC CAAGAAACTG
11281 AAGAGTGTGA AAGAGCTGCT GGGGATCACC ATCATGGAAA GAAGCAGCTT CGAGAAGAAT
11341 CCCATCGACT TTCTGGAAGC CAAGGGCTAC AAAGAAGTGA AAAAGGACCT GATCATCAAG
11401 CTGCCTAAGT ACTCCCTGTT CGAGCTGGAA AACGGCCGGA AGAGAATGCT GGCCTCTGCC
11461 GGCGAACTGC AGAAGGGAAA CGAACTGGCC CTGCCCTCCA AATATGTGAA CTTCCTGTAC
11521 CTGGCCAGCC ACTATGAGAA GCTGAAGGGC TCCCCCGAGG ATAATGAGCA GAAACAGCTG
11581 TTTGTGGAAC AGCACAAACA CTACCTGGAC GAGATCATCG AGCAGATCAG CGAGTTCTCC
11641 AAGAGAGTGA TCCTGGCCGA CGCTAATCTG GACAAGGTGC TGAGCGCCTA CAACAAGCAC
11701 AGAGACAAGC CTATCAGAGA GCAGGCCGAG AATATCATCC ACCTGTTTAC CCTGACCAAT
11761 CTGGGAGCCC CTGCCGCCTT CAAGTACTTT GACACCACCA TCGACCGGAA GAGGTACACC
11821 AGCACCAAAG AGGTGCTGGA CGCCACCCTG ATCCACCAGA GCATCACCGG CCTGTACGAG
11881 ACACGGATCG ACCTGTCTCA GCTGGGAGGC GACGCCTATC CCTATGACGT GCCCGATTAT
11941 GCCAGCCTGG GCAGCGGCTC CCCCAAGAAA AAACGCAAGG TGGAAGATCC TAAGAAAAAG
12001 CGGAAAGTGG ACGGCATTGG TAGTGGGAGC AACGGCAGCA GCGGATCCaa cggtccgGGT
12061 GGATCTGGAG GTGGAGGTTC TGGAGGAGAA GAACTTTTGA GCAAGAATTA TCATCTTGAG
12121 AACGAAGTGG CTCGTCTTAA GAAAGGTTCT GGCAGTGGAG AAGAACTGCT TTCAAAGAAT
12181 TACCACCTGG AAAATGAGGT AGCTAGACTG AAAAAGGGGA GCGGAAGTGG GGAGGAGTTG
12241 CTGAGCAAAA ATTATCATTT GGAGAACGAA GTAGCACGAC TAAAGAAAGG GTCCGGATCG
12301 GGTGAGGAGT TACTCTCGAA AAATTATCAT CTCGAAAACG AAGTGGCTCG GCTAAAAAAG
12361 GGCAGTGGTT CTGGAGAAGA GCTATTATCT AAAAACTACC ACCTCGAAAA TGAGGTGGCA
12421 CGCTTAAAAA AGGGAAGTGG CAGTGGTGAA GAGCTACTAT CCAAGAATTA TCATCTTGAG
12481 AACGAGGTAG CGCGTTTGAA GAAGGGTTCC GGCTCAGGAG AGGAACTGCT CTCGAAGAAC
12541 TATCATCTTG AAAATGAGGT CGCTCGATTA AAAAAGGGAT CGGGCAGTGG TGAGGAACTA
12601 CTTTCAAAGA ATTACCACCT CGAAAACGAA GTAGCTCGAT TAAAGAAAGG TTCAGGGTCG
12661 GGTGAAGAAT TACTGAGTAA AAATTATCAT CTGGAAAATG AGGTAGCGAG ACTAAAAAAG
12721 GGGAGTGGTT CTGGCGAAGA GTTGCTATCG AAAAATTATC ATCTTGAGAA CGAAGTTGCT
12781 AGGCTCAAAA AGGGCTCAGG CTCAGGCGAG GAGTTGCTCT CGAAAAACTA CCACTTGGAA
12841 AATGAGGTCG CGAGGTTGAA AAAGGGGAGC GGGTCGGGCG AGGAGTTATT GAGCAAAAAC
12901 TATCATTTAG AGAACGAAGT CGCGCGCTTA AAGAAAGGCT CGGGCTCGGG CGAAGAACTC
12961 TTATCGAAGA ACTACCACCT CGAAAATGAG GTCGCCAGGT TGAAAAAGGG CAGTGGCAGC
13021 GGGGAGGAAC TCTTGAGCAA GAACTACCAC TTGGAGAATG AGGTCGCGAG ATTGAAGAAA
13081 GGGTCGGGGA GCGGCGAGGA ATTGCTCAGC AAGAATTATC ATTTGGAGAA CGAAGTCGCC
13141 AGGCTCAAGA AAGGCTCGGG GTCGGGGGAG GAATTGTTGA GTAAAAACTA CCACTTGGAA
13201 AATGAAGTCG CCAGGCTCAA AAAAGGGAGT GGGAGCGGCG AAGAGTTATT GAGCAAAAAT
13261 TACCACTTGG AGAACGAAGT GGCAAGGCTC AAGAAAGGGA GCGGCAGCGG GGAGGAGCTC
13321 TTATCGAAGA ACTACCACTT AGAGAATGAA GTCGCCCGCT TGAAGAAAGG CTCGGGGAGC
13381 GGGGAAGAGC TCTTGAGCAA GAACTACCAC TTGGAAAATG AGGTGGCGCG CTTGAAGAAA
13441 GGGAGCGGGA GCGGGGAAGA GTTACTATCT AAGAATTATC ATCTCGAGAA CGAGGTGGCT
13501 CGACTAAAGA AGGGCTCCGG CAGTGGGGAG GAACTCCTGT CGAAGAACTA TCATCTTGAA
13561 AATGAGGTTG CAAGACTTAA AAAGGGGTCC GGATCAGGTG AGGAACTACT CAGTAAGAAT
13621 TACCACCTGG AAAACGAAGT TGCACGTTTG AAGAAAGGAT CAGGATCAGG CGAAGAACTG
13681 CTCTCAAAAG ATTATCATTT GGAAAATGAG GTTGCACGTT TAAAAAAGGG AAGTGGCAGT
13741 GGTGAGGAAC TTCTGTCGAA AAATTATCAT CTCGAGAATG AAGTAGCCCG ACTTAAAAAG
13801 GGTTCTGGCT CGGGTcagcg gccgcaaGGT GGAGGTGGAC CCAAGAAGAA GCGCAAGGTG
13861 GGAAGCGGAG CTACTAACTT CAGCCTGCTG AAGCAGGCTG GAGACGTGGA GGAGAACCCT
13921 GGACCTGCAC CAGGAAGTAT GAGCGAGCTG ATTAAGGAGA ACATGCACAT GAAGCTGTAC
13981 ATGGAGGGCA CCGTGGACAA CCATCACTTC AAGTGCACAT CCGAGGGCGA AGGCAAGCCC
14041 TACGAGGGCA CCCAGACCAT GAGAATCAAG GTGGTCGAGG GCGGCCCTCT CCCCTTCGCC
14101 TTCGACATCC TGGCTACTAG CTTCCTCTAC GGCAGCAAGA CCTTCATCAA CCACACCCAG
14161 GGCATCCCCG ACTTCTTCAA GCAGTCCTTC CCTGAGGGCT TCACATGGGA GAGAGTCACC
14221 ACATACGAAG ACGGGGGCGT GCTGACCGCT ACCCAGGACA CCAGCCTCCA GGACGGCTGC
14281 CTCATCTACA ACGTCAAGAT CAGAGGGGTG AACTTCACAT CCAACGGCCC TGTGATGCAG
14341 AAGAAAACAC TCGGCTGGGA GGCCTTCACC GAGACGCTGT ACCCCGCTGA CGGCGGCCTG
14401 GAAGGCAGAA ACGACATGGC CCTGAAGCTC GTGGGCGGGA GCCATCTGAT CGCAAACATC
14461 AAGACCACAT ATAGATCCAA GAAACCCGCT AAGAACCTCA AGATGCCTGG CGTCTACTAT
14521 GTGGACTACA GACTGGAAAG AATCAAGGAG GCCAACAACG AGACCTACGT CGAGCAGCAC
14581 GAGGTGGCAG TGGCCAGATA CTGCGACCTC CCTAGCAAAC TGGGGCACAA GCTTAATTAA
14641 gcggccgcga ctctagagtc gacctgcagg GATCGATCAC TGAggtacct ttaagaccaa
14701 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
14761 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
14821 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
14881 aataaagctt gccttgagtg cttcaagtag t
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