| 质粒名称: | pC-GoldyTALEN |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 38143 |
| 存储实验室: | Daniel Carlson |
|
载体骨架基本信息 |
|
| 载体名称: | pBSK |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4000 bp |
| 完整质粒大小: | 5702 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GoldyTALEN |
| 物种来源: | 合成 |
| 插入大小: | 1800 bp |
| 基因突变: | Truncate at N and C terminous |
| 启动子 | miniCaggs |
| 标签/融合蛋白: | FokI homodimer（C端） AcV5（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Esp31(在克隆期间被破坏) |
| 3' 克隆位点: | Esp31(在克隆期间被破坏) |
| 5' 测序引物 | CGTGACCGCAATGGAGGC |
| 3' 测序引物 | CACTGCATCCATGGCAGG |
|
文献引用方法 |
|
| 材料和方法部分: | pC-GoldyTALEN was a gift from Daniel Carlson (Addgene plasmid # 38143) |
| 参考文献部分: | Efficient TALEN-mediated gene knockout in livestock. Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC. Proc Natl Acad Sci U S A. 2012 Oct 1. 10.1073/pnas.1211446109 PubMed 23027955 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pC-GoldyTALEN 5701 bp DNA SYN
DEFINITION pC-GoldyTALEN
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5701
/organism="pC-GoldyTALEN"
/mol_type="other DNA"
misc_feature 108..395
/label="CAG_enhancer"
misc_feature 940..959
/label="pCAG_F_primer"
CDS 1047..1688
/label="ORF frame 3"
misc_feature 1062..1082
/label="SV40 NLS"
misc_feature 1068..1082
/label="NLS"
misc_feature complement(1544..1699)
/label="lacZ_a"
misc_feature 1670..1692
/label="M13_pUC_fwd_primer"
promoter 1685..1701
/label="M13_forward20_primer"
promoter 1711..1729
/label="T7_promoter"
promoter complement(1776..1795)
/label="T3_promoter"
promoter complement(1812..1830)
/label="M13_reverse_primer"
misc_feature complement(1829..1851)
/label="M13_pUC_rev_primer"
promoter complement(1865..1894)
/label="lac_promoter"
CDS 1909..2709
/label="ORF frame 1"
misc_feature complement(2788..2807)
/label="EBV_rev_primer"
misc_feature complement(2993..3012)
/label="EBV_rev_primer"
rep_origin complement(3403..3709)
/label="f1_origin"
promoter 3788..3816
/label="AmpR_promoter"
gene 3858..4718
/label="Ampicillin"
/gene="Ampicillin"
CDS 3858..4718
/label="ORF frame 3"
rep_origin 4873..5492
/label="pBR322_origin"
ORIGIN
1 tcattaatgc agctgagatc ttaagggatt actagtttaa acctagttat taatagtaat
61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc
421 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
481 ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg cgcgccaggc
541 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat
601 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat
661 aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc
721 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
781 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
841 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagctaga gcctctgcta
901 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
961 tgtctcatca ttttggcaaa gaattttaag ctaggatcta actcgagaaa gatattgtat
1021 atatcgtaac aataggaggt tcaacaatgg cttcctcccc tccaaagaaa aagagaaagg
1081 ttagttggaa ggacgcaagt ggttggtcta gagtggatct acgcacgctc ggctacagtc
1141 agcagcagca agagaagatc aaaccgaagg tgcgttcgac agtggcgcag caccacgagg
1201 cactggtggg ccatgggttt acacacgcgc acatcgttgc gctcagccaa cacccggcag
1261 cgttagggac cgtcgctgtc acgtatcagc acataatcac ggcgttgcca gaggcgacac
1321 acgaagacat cgttggcgtc ggcaaacagt ggtccggcgc acgcgccctg gaggccttgc
1381 tcacggatgc gggggagttg agaggtccgc cgttacagtt ggacacaggc caacttgtga
1441 agattgcaaa acgtggcggc gtgaccgcaa tggaggcagt gcatgcatcg cgcaatgcac
1501 tgacgggtgc ccccctggag acgggcgccg ctacagggcg cgtcccattc gccattcagg
1561 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
1621 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
1681 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
1741 accgggcccc ccctcgaggt cctccagctt ttgttccctt tagtgagggt taattgcgcg
1801 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
1861 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
1921 actcacatta attgcgttgc gctcactgcc cgctttccac cggtcgtctc caacgaccac
1981 ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa aaagggattg
2041 ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg cacgtcccat
2101 cgcgttgcct ctagatccca gctagtgaaa tctgaattgg aagagaagaa atctgaactt
2161 agacataaat tgaaatatgt gccacatgaa tatattgaat tgattgaaat cgcaagaaat
2221 tcaactcagg atagaatcct tgaaatgaag gtgatggagt tctttatgaa ggtttatggt
2281 tatcgtggta aacatttggg tggatcaagg aaaccagacg gagcaattta tactgtcgga
2341 tctcctattg attacggtgt gatcgttgat actaaggcat attcaggagg ttataatctt
2401 ccaattggtc aagcagatga aatgcaaaga tatgtcgaag agaatcaaac aagaaacaag
2461 catatcaacc ctaatgaatg gtggaaagtc tatccatctt cagtaacaga atttaagttc
2521 ttgtttgtga gtggtcattt caaaggaaac tacaaagctc agcttacaag attgaatcat
2581 atcactaatt gtaatggagc tgttcttagt gtagaagagc ttttgattgg tggagaaatg
2641 attaaagctg gtacattgac acttgaggaa gtgagaagga aatttaataa cggtgagata
2701 aacttttaat aggagctcgc ccggggatct aattcaatta gagactaatt caattagagc
2761 taattcaatt aggatccaga attcattgat gagtttggac aaaccacaac tagaatgcag
2821 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
2881 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
2941 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtca ttgatgagtt
3001 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
3061 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
3121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
3181 ctacaaatgt ggtcattcag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
3241 caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat
3301 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
3361 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
3421 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
3481 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
3541 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
3601 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
3661 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
3721 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
3781 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
3841 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
3901 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
3961 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
4021 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
4081 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
4141 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
4201 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
4261 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
4321 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
4381 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
4441 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
4501 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
4561 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
4621 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
4681 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
4741 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
4801 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
4861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
4921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
4981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
5041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
5101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
5161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
5221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
5281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
5341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
5401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
5461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
5521 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
5581 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
5641 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
5701 t
//
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