| 出品公司: | Promega |
|---|---|
| 载体名称: | PinPoint Xa-3 |
| 质粒类型: | 大肠杆菌表达载体；蛋白纯化 |
| 表达水平: | 高 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3333 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | V2061 |
| 稳定性: | 瞬表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
PinPoint Xa-3载体质粒基本信息
PinPoint Xa-3质粒图谱载体图谱和PinPoint Xa-3载体序列质粒序列多克隆位点信息
PinPoint Xa-3质粒载体简介
PinPoint Xa-3质粒序列载体序列
LOCUS PinPoint Xa-3 3333 bp DNA SYN
DEFINITION PinPoint Xa-3
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3333
/organism="PinPoint Xa-3"
/mol_type="other DNA"
misc_feature 1..386
/label="biotin_Pinpoint_Xa-1"
CDS 1..468
/label="ORF frame 1"
/translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
misc_feature complement(453..470)
/label="Sp6_primer"
/translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
misc_feature 670..692
/label="pGEX_3_primer"
/translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
promoter 851..879
/label="AmpR_promoter"
/translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
gene 921..1781
/label="Ampicillin"
/gene="Ampicillin"
/translation="MKLKVTVNGTAYDVDVDVDKSHENPMGTILFGGGTGGAPAPAAG
GAGAGKAGEGEIPAPLAGTVSKILVKEGDTVKAGQTVLVLEAMKMETEINAPTDGKVE
KVLVKERDAVQGGQGLIKIGDLELIEGREKLQLGSGTDIRSPGAAAIWFYSVT*"
CDS 921..1781
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 1936..2555
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 2798..2816
/label="T7_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 2940..2959
/label="pBRrevBam_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 3211..3239
/label="tac_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3251..3273
/label="M13_pUC_rev_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 atgaaactga aggtaacagt caacggcact gcgtatgacg ttgacgttga cgtcgacaag
61 tcacacgaaa acccgatggg caccatcctg ttcggcggcg gcaccggcgg cgcgccggca
121 ccggcagcag gtggcgcagg cgccggtaag gccggagagg gcgagattcc cgctccgctg
181 gccggcaccg tctccaagat cctcgtgaag gagggtgaca cggtcaaggc tggtcagacc
241 gtgctcgttc tcgaggccat gaagatggag accgagatca acgctcccac cgacggcaag
301 gtcgagaagg tcctggtcaa ggagcgtgac gcggtgcagg gcggtcaggg tctcatcaag
361 atcggggatc tcgagctcat cgaaggtcgc gaaaagcttc agctgggatc cggtaccgat
421 atcagatctc ccggggcggc cgcgatctgg ttctatagtg tcacctaaat cgtatgtgta
481 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tccatatggt
541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
601 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
661 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
1021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
1081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
1141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
1201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
1261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
1321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
1381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
1441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
1501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
1561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
1621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
1681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
1741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
1801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
1861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
1921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
1981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
2041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
2101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
2161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
2221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
2281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
2341 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
2401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
2461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
2521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
2581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
2641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
2701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
2761 cgattcatta atgcaggtta acctggctta tcgaaattaa tacgactcac tatagggaga
2821 ccggcctcga gcagcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
2881 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
2941 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
3001 atgcgtccgg cgtagaggat cgatccgggc ttatcgactg cacggtgcac caatgcttct
3061 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa
3121 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
3181 ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg
3241 tggaattgtg agcggataac aatttcacac aggaaacaga attcccagct tggctgcaga
3301 accattccat tcgttgatcc gggagtaact cac
//
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