| 质粒名称: | pRC_RR_VP2/3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 65725 |
| 存储实验室: | Junghae Suh |
|
载体骨架基本信息 |
|
| 载体名称: | AAV2_VP2_VP3 only |
| 载体出品公司: | -- |
| 完整质粒大小: | 7276 bp |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | empty |
| 物种来源: | -- |
| 插入大小: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pRC_RR_VP2/3 was a gift from Junghae Suh (Addgene plasmid # 65725 ; http://n2t.net/addgene:65725 ; RRID:Addgene_65725) |
| 参考文献部分: | Random Insertion of mCherry Into VP3 Domain of Adeno-associated Virus Yields Fluorescent Capsids With no Loss of Infectivity. Judd J, Wei F, Nguyen PQ, Tartaglia LJ, Agbandje-McKenna M, Silberg JJ, Suh J. Mol Ther Nucleic Acids. 2012 Nov 13;1:e54. doi: 10.1038/mtna.2012.46. 10.1038/mtna.2012.46 PubMed 23629029 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRC_RR_VP2/3 7276 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION wt VP2/VP3 only to participate in capsid assembly; no ITR's.
ACCESSION .
VERSION .
KEYWORDS pRC_RR_VP2/3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7276)
AUTHORS Judd J, Wei F, Nguyen PQ, Tartaglia LJ, Agbandje-McKenna M, Silberg
JJ, Suh J
TITLE Random Insertion of mCherry Into VP3 Domain of Adeno-associated
Virus Yields Fluorescent Capsids With no Loss of Infectivity.
JOURNAL Mol Ther Nucleic Acids. 2012 Nov 13;1:e54. doi:
10.1038/mtna.2012.46.
PUBMED 23629029
REFERENCE 2 (bases 1 to 7276)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7276
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(140..161)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 352..371
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 585..607
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 599..616
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 626..645
/label=T7
/note="T7 promoter, forward primer"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 669..685
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 670..686
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(720..736)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(720..736)
/label=pBluescriptSK
/note="For pBluescript vector"
CDS 4798..4809
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
primer_bind complement(5088..5108)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(5088..5106)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5127..5143)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5127..5143)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5140..5162)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5151..5167
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5175..5205)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5220..5241
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5358..5375)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5529..6117)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5609..5628)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6288..7148)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6911..6930
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7149..7253)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
721 atccactagt tctagagtcc tgtattagag gtcacgtgag tgttttgcga cattttgcga
781 caccatgtgg tcacgctggg tatttaagcc cgagtgagca cgcagggtct ccattttgaa
841 gcgggaggtt tgaacgcgca gccgccatgc cggggtttta cgagattgtg attaaggtcc
901 ccagcgacct tgacgagcat ctgcccggca tttctgacag ctttgtgaac tgggtggccg
961 agaaggaatg ggagttgccg ccagattctg acatggatct gaatctgatt gagcaggcac
1021 ccctgaccgt ggccgagaag ctgcagcgcg actttctgac ggaatggcgt cgtgtgagta
1081 aggccccgga ggcccttttc tttgtgcaat ttgagaaggg agagagctac ttccacatgc
1141 acgtgctcgt ggaaaccacc ggggtgaaat ctatggtttt gggacgtttc ctgagtcaga
1201 ttcgcgaaaa actgattcag agaatttacc gcgggatcga gccgactttg ccaaactggt
1261 tcgcggtcac aaagaccaga aatggtgccg gaggcgggaa caaggtggtg gatgagtgct
1321 acatccccaa ttacttgctc cccaaaaccc agcctgagct ccagtgggcg tggactaata
1381 tggaacagta tttaagcgcc tgtttgaatc tcacggagcg taaacggttg gtggcgcagc
1441 atctgacgca cgtgtcgcag acgcaggagc agaacaaaga gaatcagaat cccaattctg
1501 atgcgccggt gatcagatca aaaacttcag ccaggtacat ggagctggtc gggtggctcg
1561 tggacaaggg gattacctcg gagaagcagt ggatacagga ggaccaggcc tcatacatct
1621 ccttcaatgc ggcctccaac tcgcggtccc aaatcaaggc tgccttggac aatgcgggaa
1681 agattatgag cctgactaaa accgcccccg actacctggt gggccagcag cccgtggagg
1741 acatttccag caatcggatt tataaaattt tggaactaaa cgggtacgat ccccaatatg
1801 cggcttccgt ctttctggga tgggccacga aaaagttcgg caagaggaac accatctggc
1861 tgtttgggcc tgcaactacc gggaagacca acatcgcgga ggccatagcc cacactgtgc
1921 ccttctacgg gtgcgtaaac tggaccaatg agaactttcc cttcaacgac tgtgtcgaca
1981 agatggtgat ctggtgggag gaggggaaga tgaccgccaa ggtcgtggag tcggccaaag
2041 ccattctcgg aggaagcaag gtgcgcgtgg accagaaatg caagtcctcg gcccagatag
2101 acccgactcc cgtgatcgtc acctccaaca ccaacatgtg cgccgtgatt gacgggaact
2161 caacgacctt cgaacaccag cagccgttgc aagaccggat gttcaaattt gaactcaccc
2221 gccgtctgga tcatgacttt gggaaggtca ccaagcagga agtcaaagac tttttccggt
2281 gggcaaagga tcacgtggtt gaggtggagc atgaattcta cgtcaaaaag ggtggagcca
2341 agaaaagacc cgcccccagt gacgcagata taagtgagcc caaacgggtg cgcgagtcag
2401 ttgcgcagcc atcgacgtca gacgcggaag cttcgatcaa ctacgcagac aggtaccaaa
2461 acaaatgttc tcgtcacgtg ggcatgaatc tgatgctgtt tccctgcaga caatgcgaga
2521 gaatgaatca gaattcaaat atctgcttca ctcacggaca gaaagactgt ttagagtgct
2581 ttcccgtgtc agaatctcaa cccgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct
2641 acattcatca tatcatggga aaggtgccag acgcttgcac tgcctgcgat ctggtcaatg
2701 tggatttgga tgactgcatc tttgaacaat aaatgattta aatcaggtaa ggctgccgat
2761 ggttatcttc cagattggct cgaggacact ctctctgaag gaataagaca gtggtggaag
2821 ctcaaacctg gcccaccacc accaaagccc gcagagcggc ataaggacga cagcaggggt
2881 cttgtgcttc ctgggtacaa gtacctcgga cccttcaacg gactcgacaa gggagagccg
2941 gtcaacgagg cagacgccgc ggccctcgag cacgacaaag cctacgaccg gcagctcgac
3001 agcggagaca acccgtacct caagtacaac cacgccgacg cggagtttca ggagcgcctt
3061 aaagaagata cgtcttttgg gggcaacctc ggacgagcag tcttccaggc gaaaaagagg
3121 gttcttgaac ctctgggcct ggttgaggaa cctgttaaga cggctccggg aaaaaagagg
3181 ccggtagagc actctcctgt ggagccagac tcctcctcgg gaaccggaaa ggcgggccag
3241 cagcctgcaa gaaaaagatt gaattttggt cagactggag acgcagactc agtacctgac
3301 ccccagcctc tcggacagcc accagcagcc ccctctggtc tgggaactaa taccatggct
3361 acaggcagtg gcgcaccaat ggcagacaat aacgagggtg ccgacggagt gggtaattcc
3421 tcgggaaatt ggcattgcga ttccacatgg atgggcgaca gagtcatcac caccagcacc
3481 cgaacctggg ccctgcccac ctacaacaac cacctctaca aacaaatttc cagccaatca
3541 ggagcctcga acgacaatca ctactttggc tacagcaccc cttgggggta ttttgacttc
3601 aacagattcc actgccactt ttcaccacgt gactggcaaa gactcatcaa caacaactgg
3661 ggattccgac ccaagagact caacttcaag ctctttaaca ttcaagtcaa agaggtcacg
3721 cagaatgacg gtacgacgac gattgccaat aaccttacca gcacggttca ggtgtttact
3781 gactcggagt accagctccc gtacgtcctc ggctcggcgc atcaaggatg cctcccgccg
3841 ttcccagcag acgtcttcat ggtgccacag tatggatacc tcaccctgaa caacgggagt
3901 caggcagtag gacgctcttc attttactgc ctggagtact ttccttctca gatgctgcgt
3961 accggaaaca actttacctt cagctacact tttgaggacg ttcctttcca cagcagctac
4021 gctcacagcc agagtctgga ccgtctcatg aatcctctca tcgaccagta cctgtattac
4081 ttgagcagaa caaacactcc aagtggaacc accacgcagt caaggcttca gttttctcag
4141 gccggagcga gtgacattcg ggaccagtct aggaactggc ttcctggacc ctgttaccgc
4201 cagcagcgag tatcaaagac atctgcggat aacaacaaca gtgaatactc gtggactgga
4261 gctaccaagt accacctcaa tggcagagac tctctggtga atccgggccc ggctatggca
4321 agccacaagg acgatgaaga aaagtttttt cctcagagcg gggttctcat ctttgggaag
4381 caaggctcag agaaaacaaa tgtggacatt gaaaaggtca tgattacaga cgaagaggaa
4441 atcaggacaa ccaatcccgt ggctacggag cagtatggtt ctgtatctac caacctccag
4501 agaggcaaca gacaagcagc taccgcagat gtcaacacac aaggcgttct tccaggcatg
4561 gtctggcagg acagagatgt gtaccttcag gggcccatct gggcaaagat tccacacacg
4621 gacggacatt ttcacccctc tcccctcatg ggtggattcg gacttaaaca ccctcctcca
4681 cagattctca tcaagaacac cccggtgcct gcgaatcctt cgaccacctt cagtgcggca
4741 aagtttgctt ccttcatcac acagtactcc acgggacagg tcagcgtgga gatcgagtgg
4801 gagctgcaga aggaaaacag caaacgctgg aatcccgaaa ttcagtacac ttccaactac
4861 aacaagtctg ttaatgtgga ctttactgtg gacactaatg gcgtgtattc agagcctcgc
4921 cccattggca ccagatacct gactcgtaat ctgtaattgg caggtcttgt taatcaataa
4981 accgtttaat tcgtttcagt tgaactttgg tctctgcgta tttctttctt atctagtttc
5041 catgctctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
5101 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
5161 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
5221 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
5281 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
5341 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
5401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
5461 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
5521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
5581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
5641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
5701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
5761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
5821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
5881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
5941 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
6001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
6061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
6121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
6181 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
6241 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
6301 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
6361 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
6421 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
6481 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
6541 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
6601 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
6661 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
6721 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
6781 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
6841 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
6901 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
6961 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
7021 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
7081 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
7141 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
7201 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
7261 ccccgaaaag tgccac
//
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