质粒名称: | pX601-mCherry |
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出品公司: | 爱迪基因 |
目录编号: | 84039 |
存储实验室: | Yuet Wai Kan |
载体骨架基本信息 |
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载体名称: | pX601 |
载体出品公司: | Feng Zhang's laboratory |
载体抗性: | 氨苄青霉素 |
空载体大小: | 7447 bp |
完整质粒大小: | 8122 bp |
载体修饰: | 在SaCas9下游插入樱桃 |
载体类型: | 哺乳细胞表达 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | mCherry |
启动子 | T2A |
标签/融合蛋白: | mCherry(插入件上的C端子) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | HindIII(未破坏) |
3' 克隆位点: | EcoR I (未破坏) |
5' 测序引物 | Cas9-HidIII-S: gccccgtcgtgaagagaagcttcatcc |
3' 测序引物 | mCherry –EcoR1-AS:cgtagaattcttacttgtacagctcgtccatgccgccggt |
文献引用方法 |
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材料和方法部分: | pX601-mCherry was a gift from Yuet Wai Kan (Addgene plasmid # 84039 ; http://n2t.net/addgene:84039 ; RRID:Addgene_84039) |
参考文献部分: | Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and beta-thalassemia. Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO, Kan YW. Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 10.1073/pnas.1612075113. Epub 2016 Sep 6. 10.1073/pnas.1612075113 PubMed 27601644 |
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pX601mCherry载体基本信息
pX601mCherry载体图谱质粒图谱和多克隆位点信息
pX601mCherry载体简介
pX601mCherry载体序列
LOCUS pX601-mCherry 8122 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Staphylococcus aureus (SaCas9) conjugated with mCherry. ACCESSION . VERSION . KEYWORDS pX601-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8122) AUTHORS Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO, Kan YW TITLE Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and beta-thalassemia. JOURNAL Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 10.1073/pnas.1612075113. Epub 2016 Sep 6. PUBMED 27601644 REFERENCE 2 (bases 1 to 8122) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8122 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" enhancer 154..533 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 534..737 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 688..708 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 756..765 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 768..788 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 813..3968 /codon_start=1 /product="Cas9 endonuclease from the Staphylococcus aureus Type II CRISPR/Cas system" /label=SaCas9 /note="generates RNA-guided double strand breaks in DNA" /translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI IKKG" CDS 3969..4016 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 4017..4070 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 4071..4781 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(4216..4234) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 4484..4503 /label=mCherry-F /note="mCherry, forward primer" primer_bind complement(4806..4823) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4812..5019 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 5027..5267 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 5027..5047 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 5198..5217 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 5296..5371 /label=Sa gRNA scaffold /note="guide RNA scaffold for the Staphylococcus aureus CRISPR/Cas9 system" repeat_region 5385..5525 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 5385..5514 /label=AAV2 ITR rep_origin 5600..6055 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5687..5706) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5897..5918 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6072..6091) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6191..6213 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6251..6269) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6337..6441 /gene="bla" /label=AmpR promoter CDS 6442..7302 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6660..6679) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7473..8061 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7962..7981 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat 181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc 541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc 601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa 661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg 721 tctatataag cagagctctc tggctaacta ccggtgccac catggcccca aagaagaagc 781 ggaaggtcgg tatccacgga gtcccagcag ccaagcggaa ctacatcctg ggcctggaca 841 tcggcatcac cagcgtgggc tacggcatca tcgactacga gacacgggac gtgatcgatg 901 ccggcgtgcg gctgttcaaa gaggccaacg tggaaaacaa cgagggcagg cggagcaaga 961 gaggcgccag aaggctgaag cggcggaggc ggcatagaat ccagagagtg aagaagctgc 1021 tgttcgacta caacctgctg accgaccaca gcgagctgag cggcatcaac ccctacgagg 1081 ccagagtgaa gggcctgagc cagaagctga gcgaggaaga gttctctgcc gccctgctgc 1141 acctggccaa gagaagaggc gtgcacaacg tgaacgaggt ggaagaggac accggcaacg 1201 agctgtccac caaagagcag atcagccgga acagcaaggc cctggaagag aaatacgtgg 1261 ccgaactgca gctggaacgg ctgaagaaag acggcgaagt gcggggcagc atcaacagat 1321 tcaagaccag cgactacgtg aaagaagcca aacagctgct gaaggtgcag aaggcctacc 1381 accagctgga ccagagcttc atcgacacct acatcgacct gctggaaacc cggcggacct 1441 actatgaggg acctggcgag ggcagcccct tcggctggaa ggacatcaaa gaatggtacg 1501 agatgctgat gggccactgc acctacttcc ccgaggaact gcggagcgtg aagtacgcct 1561 acaacgccga cctgtacaac gccctgaacg acctgaacaa tctcgtgatc accagggacg 1621 agaacgagaa gctggaatat tacgagaagt tccagatcat cgagaacgtg ttcaagcaga 1681 agaagaagcc caccctgaag cagatcgcca aagaaatcct cgtgaacgaa gaggatatta 1741 agggctacag agtgaccagc accggcaagc ccgagttcac caacctgaag gtgtaccacg 1801 acatcaagga cattaccgcc cggaaagaga ttattgagaa cgccgagctg ctggatcaga 1861 ttgccaagat cctgaccatc taccagagca gcgaggacat ccaggaagaa ctgaccaatc 1921 tgaactccga gctgacccag gaagagatcg agcagatctc taatctgaag ggctataccg 1981 gcacccacaa cctgagcctg aaggccatca acctgatcct ggacgagctg tggcacacca 2041 acgacaacca gatcgctatc ttcaaccggc tgaagctggt gcccaagaag gtggacctgt 2101 cccagcagaa agagatcccc accaccctgg tggacgactt catcctgagc cccgtcgtga 2161 agagaagctt catccagagc atcaaagtga tcaacgccat catcaagaag tacggcctgc 2221 ccaacgacat cattatcgag ctggcccgcg agaagaactc caaggacgcc cagaaaatga 2281 tcaacgagat gcagaagcgg aaccggcaga ccaacgagcg gatcgaggaa atcatccgga 2341 ccaccggcaa agagaacgcc aagtacctga tcgagaagat caagctgcac gacatgcagg 2401 aaggcaagtg cctgtacagc ctggaagcca tccctctgga agatctgctg aacaacccct 2461 tcaactatga ggtggaccac atcatcccca gaagcgtgtc cttcgacaac agcttcaaca 2521 acaaggtgct cgtgaagcag gaagaaaaca gcaagaaggg caaccggacc ccattccagt 2581 acctgagcag cagcgacagc aagatcagct acgaaacctt caagaagcac atcctgaatc 2641 tggccaaggg caagggcaga atcagcaaga ccaagaaaga gtatctgctg gaagaacggg 2701 acatcaacag gttctccgtg cagaaagact tcatcaaccg gaacctggtg gataccagat 2761 acgccaccag aggcctgatg aacctgctgc ggagctactt cagagtgaac aacctggacg 2821 tgaaagtgaa gtccatcaat ggcggcttca ccagctttct gcggcggaag tggaagttta 2881 agaaagagcg gaacaagggg tacaagcacc acgccgagga cgccctgatc attgccaacg 2941 ccgatttcat cttcaaagag tggaagaaac tggacaaggc caaaaaagtg atggaaaacc 3001 agatgttcga ggaaaagcag gccgagagca tgcccgagat cgaaaccgag caggagtaca 3061 aagagatctt catcaccccc caccagatca agcacattaa ggacttcaag gactacaagt 3121 acagccaccg ggtggacaag aagcctaata gagagctgat taacgacacc ctgtactcca 3181 cccggaagga cgacaagggc aacaccctga tcgtgaacaa tctgaacggc ctgtacgaca 3241 aggacaatga caagctgaaa aagctgatca acaagagccc cgaaaagctg ctgatgtacc 3301 accacgaccc ccagacctac cagaaactga agctgattat ggaacagtac ggcgacgaga 3361 agaatcccct gtacaagtac tacgaggaaa ccgggaacta cctgaccaag tactccaaaa 3421 aggacaacgg ccccgtgatc aagaagatta agtattacgg caacaaactg aacgcccatc 3481 tggacatcac cgacgactac cccaacagca gaaacaaggt cgtgaagctg tccctgaagc 3541 cctacagatt cgacgtgtac ctggacaatg gcgtgtacaa gttcgtgacc gtgaagaatc 3601 tggatgtgat caaaaaagaa aactactacg aagtgaatag caagtgctat gaggaagcta 3661 agaagctgaa gaagatcagc aaccaggccg agtttatcgc ctccttctac aacaacgatc 3721 tgatcaagat caacggcgag ctgtatagag tgatcggcgt gaacaacgac ctgctgaacc 3781 ggatcgaagt gaacatgatc gacatcacct accgcgagta cctggaaaac atgaacgaca 3841 agaggccccc caggatcatt aagacaatcg cctccaagac ccagagcatt aagaagtaca 3901 gcacagacat tctgggcaac ctgtatgaag tgaaatctaa gaagcaccct cagatcatca 3961 aaaagggcaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaaggagg 4021 gcagaggatc cctgctaaca tgtggtgacg tcgaggagaa tcctggccca atggtgagca 4081 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg 4141 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 4201 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg 4261 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca 4321 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 4381 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca 4441 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga 4501 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg 4561 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga 4621 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt 4681 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg 4741 gccgccactc caccggcggc atggacgagc tgtacaagta agaattccta gagctcgctg 4801 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 4861 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 4921 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 4981 gggggaggat tgggaagaga atagcaggca tgctggggag gtacctgagg gcctatttcc 5041 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa 5101 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct 5161 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac 5221 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggaga 5281 ccacggcagg tctcagtttt agtactctgg aaacagaatc tactaaaaca aggcaaaatg 5341 ccgtgtttat ctcgtcaact tgttggcgag atttttgcgg ccgcaggaac ccctagtgat 5401 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt 5461 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct 5521 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat 5581 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 5641 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 5701 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 5761 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt 5821 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 5881 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 5941 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 6001 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg 6061 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca 6121 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 6181 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 6241 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 6301 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 6361 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 6421 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 6481 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 6541 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 6601 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 6661 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 6721 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 6781 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 6841 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 6901 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 6961 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 7021 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 7081 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 7141 ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc 7201 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 7261 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 7321 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 7381 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 7441 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 7501 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 7561 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 7621 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 7681 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 7741 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 7801 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 7861 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 7921 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 7981 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 8041 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 8101 tgctggcctt ttgctcacat gt //