质粒名称: | pCasSA |
---|---|
出品公司: | addgene |
目录编号: | 98211 |
存储实验室: | Quanjiang Ji |
载体骨架基本信息 |
|
载体名称: | 未知 |
完整质粒大小: | 10240bp |
载体类型: | 细菌细胞表达 |
筛选标记: | Chloramphenicol (in S. aureus) |
载体抗性: | 氨苄霉素 |
生长温度: | 30 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
|
克隆方法: | 未知 |
文献引用方法 |
|
材料和方法部分: |
pCasSA was a gift from Quanjiang Ji (Addgene plasmid # 98211 ; http://n2t.net/addgene:98211 ; RRID:Addgene_98211)
|
参考文献部分: | Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System. Chen W, Zhang Y, Yeo WS, Bae T, Ji Q. J Am Chem Soc. 2017 Mar 15;139(10):3790-3795. doi: 10.1021/jacs.6b13317. Epub 2017 Mar 2. 10.1021/jacs.6b13317 PubMed 28218837 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCasSA 10240 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus. ACCESSION . VERSION . KEYWORDS pCasSA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10240) AUTHORS Chen W, Zhang Y, Yeo WS, Bae T, Ji Q TITLE Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System. JOURNAL J Am Chem Soc. 2017 Mar 15;139(10):3790-3795. doi: 10.1021/jacs.6b13317. Epub 2017 Mar 2. PUBMED 28218837 REFERENCE 2 (bases 1 to 10240) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..10240 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 79..667 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 568..587 /label=pBR322ori-F /note="pBR322 origin, forward primer" misc_RNA 805..880 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" CDS 1121..5227 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" primer_bind 5324..5342 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(5380..5402) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 5502..5521 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5715..5737 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 5729..5746 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 5730..5746 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 9125..9141 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9125..9141 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 9312..10106 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(9366..9385) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 9976..9995 /label=Neo-F /note="Neomycin resistance gene, forward primer" terminator 10137..10231 /label=lambda t0 terminator /note="transcription terminator from phage lambda" ORIGIN 1 agtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 61 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 121 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 181 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 241 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 301 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 361 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 421 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 481 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 541 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 601 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 661 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 721 tcacatgttc tttcctgcgt tgtcgacaga gtttgcaaaa tatacagggg attatatata 781 atggaaacga gaccattggt ctcagtttta gagctagaaa tagcaagtta aaataaggct 841 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgag atctgtccat 901 acccatggtc tagaatgctc gagtcagaaa aatatacctg tatctttttt caaaagcaaa 961 catgctttgg gtaaacatgt aggtattaac gtcaatgcga caatagtagc attgattaaa 1021 tgagaattag taagtgtttt acttactaaa ttttatttaa cctaaaaatg aaccacctgg 1081 atgtgtggga ttaaaaagtg aagagaggag gacatatcac atggataaga aatactcaat 1141 aggcttagat atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt 1201 tccgtctaaa aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct 1261 tataggggct cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac 1321 agctcgtaga aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc 1381 aaatgagatg gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt 1441 ggaagaagac aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc 1501 ttatcatgag aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga 1561 taaagcggat ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca 1621 ttttttgatt gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca 1681 gttggtacaa acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga 1741 tgctaaagcg attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc 1801 tcagctcccc ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg 1861 tttgacccct aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc 1921 aaaagatact tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc 1981 tgatttgttt ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag 2041 agtaaatact gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga 2101 acatcatcaa gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta 2161 taaagaaatc ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc 2221 tagccaagaa gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga 2281 ggaattattg gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa 2341 cggctctatt ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga 2401 agacttttat ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg 2461 aattccttat tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg 2521 gaagtctgaa gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc 2581 agctcaatca tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt 2641 actaccaaaa catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt 2701 caaatatgtt actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc 2761 cattgttgat ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga 2821 ttatttcaaa aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt 2881 taatgcttca ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt 2941 ggataatgaa gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga 3001 agatagggag atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt 3061 gatgaaacag cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat 3121 taatggtatt agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg 3181 ttttgccaat cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga 3241 cattcaaaaa gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt 3301 agctggtagc cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt 3361 ggtcaaagta atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa 3421 tcagacaact caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg 3481 tatcaaagaa ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca 3541 aaatgaaaag ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga 3601 attagatatt aatcgtttaa gtgattatga tgtcgatcac attgttccac aaagtttcct 3661 taaagacgat tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc 3721 ggataacgtt ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct 3781 aaacgccaag ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg 3841 tttgagtgaa cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat 3901 cactaagcat gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga 3961 taaacttatt cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg 4021 aaaagatttc caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc 4081 gtatctaaat gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga 4141 gtttgtctat ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca 4201 agaaataggc aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa 4261 aacagaaatt acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg 4321 ggaaactgga gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt 4381 gtccatgccc caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa 4441 ggagtcaatt ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga 4501 tccaaaaaaa tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc 4561 taaggtggaa aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac 4621 aattatggaa agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata 4681 taaggaagtt aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga 4741 aaacggtcgt aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc 4801 tctgccaagc aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg 4861 tagtccagaa gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga 4921 tgagattatt gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt 4981 agataaagtt cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga 5041 aaatattatt catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt 5101 tgatacaaca attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct 5161 tatccatcaa tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg 5221 tgactgatgg ctggttggcg tactgttgtg gtaaataccc atgggtatgg acagttttcc 5281 cgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 5341 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 5401 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 5461 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 5521 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 5581 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 5641 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 5701 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg 5761 cctgcagaac ggattgttga tgattacgaa aatattaaga gcacagacta ttacacagaa 5821 aatcaagaat taaaaaaacg tagagagagt ttgaaagaag tagtgaatac atggaaagag 5881 gggtatcacg aaaaaagtaa agaggttaat aaattaaagc gagagaatga tagtttgaat 5941 gagcagttga atgtatcaga gaaatttcaa gatagtacag tgactttata tcgtgctgcg 6001 agggcgaatt tccctgggtt tgagaaaggg tttaataggc ttaaagagaa attctttaat 6061 gattccaaat tcgagcgtgt gggacagttt atggatgttg tacaggataa tgtccagaag 6121 gtcgatagaa agcgtgagaa acagcgtaca gacgatttag agatgtagag gtacttttat 6181 gccgagaaaa ctttttgcgt gtgacagtcc ttaaaatata cttagagcgt aagcgaaagt 6241 agtagcgaca gctattaact ttcggttgca aagctctagg atttttaatg gacgcagcgc 6301 atcacacgca aaaaggaaat tggaataaat gcgaaatttg agatgttaat taaagacctt 6361 tttgaggtct ttttttctta gatttttggg gttatttagg ggagaaaaca taggggggta 6421 ctacgacctc ccccctaggt gtccattgtc cattgtccaa acaaataaat aaatattggg 6481 tttttaatgt taaaaggttg ttttttatgt taaagtgaaa aaaacagatg ttgggaggta 6541 cagtgatggt tgtagataga aaagaagaga aaaaagttgc tgttacttta agacttacaa 6601 cagaagaaaa tgagatatta aatagaatca aagaaaaata taatattagc aaatcagatg 6661 caaccggtat tctaataaaa aaatatgcaa aggaggaata cggtgcattt taaacaaaaa 6721 aagatagaca gcactggcat gctgcctatc tatgactaaa ttttgttaaa tgtattagca 6781 ccgttattat atcatgagcg aaaatgtaat aaaagaaact gaaaacaaga aaaattcaag 6841 aggacgtaat tggacatttg ttttatatcc agaatcagca aaagccgagt ggttagagta 6901 tttaaaagag ttacacattc aatttgtagt gtctccatta catgataggg atactgatac 6961 agaagatagg atgaaaaaag agcattatca tattctagtg atgtatgagg gtaataaatc 7021 ttatgaacag ataaaaataa ttacagaaga attgaatgcg actattccgc agattgcagg 7081 aagtgtgaaa ggtcttgtga gatatatgct tcacatggac gatcctaata aatttaaata 7141 tcaaaaagaa gatatgatag tttatggcgg tgtagatgtt gatgaattat taaagaaaac 7201 aacaacagat agatataaat taattaaaga aatgattgag tttattgatg aacaaggaat 7261 cgtagaattt aagagtttaa tggattatgc aatgaagttt aaatttgatg attggttccc 7321 gcttttatgt gataactcgg cgtatgttat tcaagaatat ataaaatcaa atcggtataa 7381 atctgaccga tagattttga atttaagagt gtcacaagac actctttttt cgcaccagcg 7441 aaaactggtt taagccgact gcgcaaaaga cataatcgat tcacaaaaaa taggcacacg 7501 aaaaacaagt taagggatgc agtttatgca tcccttaact tacttattaa ataatttata 7561 gctattgaaa agagataaga attgttcaaa gctaatattg tttaaatcgt caattcctgc 7621 atgttttaag gaattgttaa attgattttt tgtaaatatt ttcttgtatt ctttgttatc 7681 ttggttaccc gtcttcttaa tatgcgtaat tgcatctact ctaaatccat caatgccttt 7741 atcaaaccac cagttcatca tttcaaatac agcatctcta acttccggat taccccaatt 7801 caaatcaggt tgttttttac tgaataaatg gaaataatat tgctcagtat tagcatcata 7861 ttcccgcgcg ccaattgagc tcccctttct atgtatgttt tttactagtc atttaaaacg 7921 atacattaat aggtacgaaa aagcaacttt ttttgcgctt aaaaccagtc ataccaataa 7981 cttaagggta actagcctcg ccggcaatag ttacccttat tatcaagata agaaagaaaa 8041 ggatttttcg ctacgctcaa atcctttaaa aaaacacaaa agaccacatt ttttaatgtg 8101 gtcttttatt cttcaactaa agcacccatt agttcaacaa acgaaaattg gataaagtgg 8161 gatattttta aaatatatat ttatgttaca gtaatattga cttttaaaaa aggattgatt 8221 ctaatgaaga aagcagacaa gtaagcctcc taaattcact ttagataaaa atttaggagg 8281 catatcaaat gaaatttaat aaaattgatt tagacaattg gaagagaaaa gagatattta 8341 atcattattt gaaccaacaa acgactttta gtataaccac agaaattgat attagtgttt 8401 tataccgaaa cataaaacaa gaaggatata aattttaccc tgcatttatt ttcttagtga 8461 caagggtgat aaactcaaat acagctttta gaactggtta caatagcgac ggagagttag 8521 gttattggga taagttagag ccactttata caatttttga tggtgtatct aaaacattct 8581 ctggtatttg gactcctgta aagaatgact tcaaagagtt ttatgattta tacctttctg 8641 atgtagagaa atataatggt tcggggaaat tgtttcccaa aacacctata cctgaaaatg 8701 ctttttctct ttctattatt ccatggactt catttactgg gtttaactta aatatcaata 8761 ataatagtaa ttaccttcta cccattatta cagcaggaaa attcattaat aaaggtaatt 8821 caatatattt accgctatct ttacaggtac atcattctgt ttgtgatggt tatcatgcag 8881 gattgtttat gaactctatt caggaattgt cagataggcc taatgactgg cttttataat 8941 atgagataat gccgactgta ctttttacag tcggttttct aatgtcacta acctgccccg 9001 ttagttgaag aaggttttta tattacagct ccagatccat atccttcttt ttctgaaccg 9061 acttctcctt tttcgcttct ttattccaat tgctttattg acgttgagcc tcggaaccgg 9121 tacccaggaa acagctatga ccatgtaata cgactcacta tacggggata tcgtcggaat 9181 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt 9241 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag 9301 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 9361 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 9421 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 9481 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 9541 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 9601 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 9661 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 9721 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 9781 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 9841 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 9901 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 9961 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 10021 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 10081 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgagagct cgcttggact 10141 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg 10201 ttgccgccgg gcgtttttta ttggtgagaa tccaagcact //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:pcDNA3/Flag-WTAP
- 下一篇:pYLsgRNA-AtU3b