LOCUS pCMV-PEmax-P2A-hMLH1dn 12117 bp ds-DNA circular SYN 29-MAR-2022
DEFINITION Mammalian expression of SpCas9 PEmax prime editor with P2A-human
MLH1dn (codon optimized).
ACCESSION .
VERSION .
KEYWORDS pCMV-PEmax-P2A-hMLH1dn
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12117)
AUTHORS Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen
C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B,
Liu DR
TITLE Enhanced prime editing systems by manipulating cellular determinants
of editing outcomes.
JOURNAL Cell. 2021 Oct 9. pii: S0092-8674(21)01065-5. doi:
10.1016/j.cell.2021.09.018.
PUBMED 34653350
REFERENCE 2 (bases 1 to 12117)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 29, 2022 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12117
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 48..67
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 301..318
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 435..456
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 471..501
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 509..525
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 514..536
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 533..549
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 533..549
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
polyA_signal 620..844
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 833..850
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
CDS complement(873..890)
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS complement(3155..3211)
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS complement(3221..3247)
/codon_start=1
/product="nuclear localization signal of human c-Myc
proto-oncogene (Dang and Lee, 1988)"
/label=c-myc NLS
/translation="PAAKRVKLD"
CDS complement(3257..3277)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(5378..5398)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(5456..9556)
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS complement(9557..9577)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(9626..9645)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(9627..9645)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(9687..9890)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(9716..9736)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 9891..10270
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 10442..10461
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 10540..10644
/gene="bla"
/label=AmpR promoter
CDS 10645..11505
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10863..10882)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 11676..147
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
61 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
121 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
181 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
241 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
301 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
361 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
421 gagcgcaacg caattaatgt gagttagctc actcattagg caccctaggc tttacacttt
481 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
541 agctatgacc atgattacgc caagctctag ctagaggtcg acggtatcga gccccagctg
601 gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct gctattgtct
661 tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta
721 ctcagacaat gcgatgcaat tttctcattt tattaggaaa ggacagtggg agtggcacct
781 tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc
841 acagtcgagg ctgatcagcg ggtttaaact caatggtgat ggtgatgatg accggttaga
901 aaaccttata aaggtcgggc aggttggcca gctgcaggat gttgccgtcc tcggtgaagt
961 gcttaggagg gagaatgtgg ctccgcaggg ccttgtacac gatgtgttcc acggtccact
1021 tccagctgtt ggggatgctt ccgggcactt cactctgctg gccagacaga gtgctctctt
1081 cagagatgta ctgttttctg atgctataga acatggcgca ctccttgctc agagactcga
1141 agcattcctt ttcctcgtcc cagttcacct ctgtagccag tctcaggatg aagatgggca
1201 ggccttccag gggaggcacg tagttatcga tcagcagagg caggccgatc aggttgcctt
1261 cctcgtcgat ttccagagaa aagtagtcgg ccagcatctc ggcctttttc ttcaggaact
1321 ccacgatgta ttcagccagc ccctctttag gtccgtcctc ttctgtccag ccgctttctg
1381 ggctatccag agccagcatg gccaggtcaa acagaggggc gggctcgctc aggcgtagca
1441 cgccgaagtt ggcgaagtcg tagatgagga tctggtagaa cagctcttcg ctcagcttgg
1501 tggtgttcag caggtacagc tttgtttgat gctgagccag ggcccattga gggttcacgc
1561 agcccacgaa gctgtgattg tgcagcatct ctctcagcac ctcgtggccc tgctcgttga
1621 tctcctcctg caggctcagc acggaggtca ggttgatgat tcttcttcta ggggtacaag
1681 cggctgtcat ttcctttctg ctgtcgtcct ccaccatttc cacgtcgctg tcctcccggt
1741 gccgttttct ggggttgctg gaggtagggc cccgcttctc gctcatttcg ctggtgccct
1801 ttgtggtatc tccctccagg ctctggttct tggcggccac ctcggcagga gctggcagct
1861 cgagcatttc ttcgtcctgc tgtctggcac ggccggagga gatatctgtc ttgtcctcgg
1921 tcacgatggc ctgaggctga gagctcagag gtttgctcag aggttgcaga aaggcgtcca
1981 gcttctgctc ccgagagtcg gtccgcacca tctggtgggc gtacacctta tcggaagaac
2041 cgctggtgct ggatgaggtc agagaggttg tggacttaac catttcgcct gaggggccgg
2101 ccaggccagg cagcagggtc tgtgtgaagt acattctgct gctgttagag cccagcagct
2161 tgctctcaat gtgctgctgc actctttcca ggatgctttc ctcgtgcagg aagtgcacct
2221 cgtgctttgt aggatgcaca ttgacgtcca cgttctgagg gctgatctcg aggctcaggt
2281 acaggaaggg gtgggtgttc ttaggcaggt aggcggcgta cactgtctcg atggctttcc
2341 gcaggctggt gctttccacc agtctgtggt tgatgaacag aagaaagatg cacttcttca
2401 cagagtaatt ggcgttgctg atgtagccgt tcatcttaaa agccagggtc ttatcttcgc
2461 agccgatctc gatcagttct ctggacacgg catttccgaa aatgcttctg atgttatcca
2521 cggtgctggc atttggcagg gtgcgcacat cggccacggt ttctccctgc ttctttacgc
2581 taaagctgat tccggcgttg tgcacggagt atctgccgac gacttccagg atcttgccgt
2641 actcctcgct gggatttttc agggcctttc tccgggtggc gatgttgtag aacagatcct
2701 ccactgttat ctgtgtaccc tggttgccgg cgcaaggctt tggcggggct ttcagcttgc
2761 cgtcgctgta gctagcgcga taagcgcatt tgccgtcggc ggttttggtg gtgatggtca
2821 catgggccac gtggctgatg ctggccaggg cctcgcctct gaagccgtag gtactgatag
2881 aggccagatc ctcaaaagac tgcagcttag atgtggtgaa ccgttcacac acgatgtcca
2941 gatcttcctt tctgatgcct gtgccgttgt cttggatctg gatcagcttc agtccgccct
3001 ccttcacgat cacctgaatg cttgtggact tggcgtccag acagttctcg atcatctcct
3061 ttattgcatt ggcaggtctc tggatcacct cgccggcggc aattctgttc accactgtct
3121 cgtccagtct ccggatgact ccagcaacga agctaggtcc agggttctcc tccacgtctc
3181 cagcctgctt cagcaggctg aagttagtag ctccgcttcc gtccagcttc actctcttag
3241 cggcagggcc agagccgact ttcctcttct tcttgggaga ctcgaattcg ctgccgtcgg
3301 cggttctttt tgagccgcca gaaggggagg agttttcaat cagcagagtg gaagtgtcgg
3361 gagtttcagt aatagcagcc tttctggcgg cctggtcggc catccgattg ccccttgcct
3421 cggcggagtg tcccttctgg tgtccaggac agtggatgat gctcagccgc ttgggcagaa
3481 acagggcctt cagcagggcc aggatctcat ccttgttctt gatctccttg ccctcagagg
3541 tcagccagcc ccggcgcctg tagatctctc cgtggatgtg tgcggtggcg aaggcatatc
3601 tggagtctgt gtacacgttc agcttcttgc cctcggccat cttcagggcc tgggtcaggg
3661 cgatcagctc ggcccgctgg gcggatgtgc ctgctggcag ggccttggcc cagatcactt
3721 ctgtctctgt ggtcactgct gctcctgcct tcctctggcc ctcctgcagc agggagcttc
3781 catcggtata ccatgtgtga tcggcgtcag gcaggggctg atcggtcagg tctggccttg
3841 ttccgtgtgc ctctgccagg atgtccagac agttgtgctg caggccctcc tctggcagag
3901 gcagcagggt ggctggattc agggccacca cagggccaaa ctgcacgcga tcggtgtcca
3961 gcagcagggc ctggtagtgt gtcatccggg cgttagacag ccagcgatct ggaggctgct
4021 tcaccagggc ctccactgcg tgtggtgcca ggatgaccag tggctgtccc atggtcagct
4081 tgccggcatc ctttgtcagc acggcgattg ctgccaccat cctcaggcat ggtggccatc
4141 ctgctgccac tgggtccagc ttcttggaca gataggccac gggccgtctc catggtccca
4201 gcttctgggt cagcacgccc ttggcgtagc cctgcttctc atccacaaac agctcgaaag
4261 gcttggtcag gtctggcagg cccagggctg gtgctgtcag cagggcctgc ttgatctcct
4321 gataggcctt ctgctggtca gggccccaat taaacagggt gcccggcttg gtcagtgggt
4381 acagaggtgc tgccatctct gcgaatcctg ggatgaacag cctgcaaaat cctgctttgc
4441 ccaggaactc cctcagctgt cttggggtct tgggtgttgg ctggcccatc actgtctcct
4501 tcctggcctc tgtcagccat ctctggccct ccttcagcag atagcccagg tacttcacct
4561 gcttctgaca gatctgggcc ttcttggcgg atgccctata tcccaggttt cccagggtct
4621 gcagcagggc gcgtgttccc tgctggcaat ccagctcgct ggtggcggcc agcagcagat
4681 cgtccacgta ctgcagcagg atcagatctg ggtgctggat ccggaaatct gccaggtcgc
4741 ggtgcagggc ctcgttaaac agtgttgggc tattcttgaa gccctgaggc aggcgtgtcc
4801 aggtcagctg gccagagatg cccatctcag ggtccctcca ctcaaaggcg aacagtggct
4861 ggctggtagg gtgcagtctc agacaaaaga aggcatcctt caggtccagc actgtatacc
4921 actggtgaga agggggcagg ccggacagca gattgtaagg gttgggcacg gtggggtgga
4981 tatcctccac cctcttgttc acctctctca gatcctgcac gggtctatag tcattggtgc
5041 ctggcttctt cacgggcagc agtggtgtgt tccaggggct ctggcatggc accaggatgc
5101 cctggtccag cagcctctgg atgtgaggct tgatgcccag tctggcctcc tgagacatag
5161 ggtactgctt gatgctcacg ggtgtagagg tggccttcag agggatgatc aggggtgcct
5221 gccgcacggc caggcccatt cctcctgtct cggcccaggc ctggggaaag tcggacagcc
5281 aggtggagcc cagggacacg tcgggttcct tgcttgtttc atgcagtcta tactcgtctt
5341 caatgttcag ggtgcttccg ccgctagagc ctccgctcac ctttcttttc ttcttagggc
5401 tctcgaattc agagccgtcg gcggtccgct tgctgccacc agagcttccg ccggagtcac
5461 ctcccagctg agacaggtcg atccgtgtct cgtacaggcc ggtgatgctc tggtggatca
5521 gggtggcgtc cagcacctct ttggtgctgg tgtacctctt ccggtcgatg gtggtgtcaa
5581 agtacttgaa ggcggcaggg gctcccagat tggtcagggt aaacaggtgg atgatattct
5641 cggcctgctc tctgatgggc ttatcccggt gcttgttgta ggcggacagc actttgtcca
5701 gattagcgtc ggccaggatc actctcttgg agaactcgct gatctgctcg atgatctcgt
5761 ccaggtagtg cttgtgctgt tccacaaaca gctgtttctg ctcattatcc tcgggggagc
5821 ccttcagctt ctcatagtgg ctggccaggt acaggaagtt cacatatttg gagggcaggg
5881 ccagttcgtt tcccttctgc agttcgccgg cagaggccag cattctcttc cggccgtttt
5941 ccagctcgaa cagggagtac ttaggcagct tgatgatcag gtcctttttc acttctttgt
6001 agcccttggc ttccagaaag tcgatgggat tcttctcgaa gctgcttctt tccatgatgg
6061 tgatccccag cagctctttc acactcttca gtttcttgga cttgcccttt tccactttgg
6121 ccaccaccag cacagaatag gccacggtgg ggctgtcgaa gccgccgtac ttcttagggt
6181 cccagtcctt ctttctggcg atcagcttat cgctgttcct cttgggcagg atagactctt
6241 tgctgaagcc gcctgtctgc acctcggtct ttttcacgat attcacttgg ggcatgctca
6301 gcactttccg cacggtggca aaatcccggc ccttatccca cacgatctcc ccggtttcgc
6361 cgtttgtctc gatcagaggc cgcttccgga tctcgccgtt ggccagggta atctcggtct
6421 tgaaaaagtt catgatgttg ctgtagaaga agtacttggc ggtagccttg ccgatttcct
6481 gctcgctctt ggcgatcatc ttccgcacgt cgtacacctt gtagtcgccg tacacgaact
6541 cgctttccag cttagggtac tttttgatca gggcggttcc cacgacggcg ttcaggtagg
6601 cgtcgtgggc gtggtggtag ttgttgatct cgcgcacttt gtaaaactgg aaatccttcc
6661 ggaaatcgga caccagcttg gacttcaggg tgatcacttt cacttcccgg atcagcttgt
6721 cattctcgtc gtacttagtg ttcatccggg agtccaggat ctgtgccacg tgctttgtga
6781 tctgccgggt ttccaccagc tgtctcttga tgaagccggc cttatccagt tcgctcaggc
6841 cgcctctctc ggccttggtc agattgtcga actttctctg ggtaatcagc ttggcgttca
6901 gcagctgccg ccagtagttc ttcatcttct tcacgacctc ttcggagggc acgttgtcgc
6961 tcttgccccg gttcttgtcg cttctggtca gcaccttgtt gtcgatggag tcgtccttca
7021 gaaagctctg aggcacgata gcgtccacat cgtagtcgga cagccggttg atgtccagtt
7081 cctggtccac gtacatatcc cgcccattct gcaggtagta caggtacagc ttctcgttct
7141 gcagctgggt gttttccacg gggtgttctt tcaggatctg gctgcccagc tctttgatgc
7201 cctcttcgat ccgcttcatt ctctcgcggc tgttcttctg tcccttctgg gtggtctggt
7261 tctctctggc catttcgatc acgatgttct cgggcttgtg ccggcccatc actttcacga
7321 gctcgtccac caccttcact gtctgcagga tgcccttctt aatggcgggg ctgccggcca
7381 gattggcaat gtgctcgtgc aggctatcgc cctggccgga cacctgggct ttctggatgt
7441 cctctttaaa ggtcaggctg tcgtcgtgga tcagctgcat gaagtttctg ttggcgaagc
7501 cgtcggactt caggaaatcc aggattgtct tgccggactg cttgtcccgg atgccgttga
7561 tcagcttccg gctcagcctg ccccagccgg tgtatctccg ccgcttcagc tgcttcatca
7621 ctttgtcgtc gaacaggtgg gcataggttt tcagccgttc ctcgatcatc tctctgtcct
7681 caaacagtgt cagggtcagc acgatatctt ccagaatgtc ctcgttttcc tcattgtcca
7741 ggaagtcctt gtccttgata attttcagca gatcgtggta tgtgcccagg gaggcgttga
7801 accgatcttc cacgccggag atttccacgg agtcgaagca ctcgattttc ttgaagtagt
7861 cctctttcag ctgcttcacg gtcactttcc ggttggtctt gaacagcagg tccacgatgg
7921 cctttttctg ctcgccgctc aggaaggcgg gctttctcat tccctcggtc acgtatttca
7981 ctttggtcag ctcgttatac acggtgaagt actcgtacag caggctgtgc ttgggcagca
8041 ccttctcgtt gggcaggttc ttatcgaagt tggtcatccg ctcgatgaag ctctgggcgg
8101 aagcgccctt gtccaccact tcctcgaagt tccagggggt gatggtttcc tcgctctttc
8161 tggtcatcca ggcgaatctg ctgtttcccc tggccagagg gcccacgtag taggggatgc
8221 ggaaggtcag gatcttctcg atcttttccc ggttgtcctt caggaatggg taaaaatctt
8281 cctgccgccg cagaatggcg tgcagctctc ccaggtggat ctggtggggg atgctgccgt
8341 tgtcgaaggt ccgctgcttc cgcagcaggt cctctctctt cagcttcacg agcagttcct
8401 cggtgccgtc catcttttcc aggatgggct tgatgaactt gtagaactct tcctggctgg
8461 ctccgccgtc aatgtagccg gcgtagccgt tcttgctctg gtcgaagaaa atctctttgt
8521 acttctcagg cagctgctgc cgcacgagag ctttcagcag ggtcaggtcc tggtggtgct
8581 cgtcgtatct cttgatcata gaggcgctca ggggggcctt ggtgatctcg gtgttcactc
8641 tcaggatgtc gctcagcagg atggcgtcgg acaggttctt ggcggccaga aacaggtcgg
8701 cgtactggtc gccgatctgg gccagcaggt tgtccaggtc gtcgtcgtag gtgtccttgc
8761 tcagctgcag tttggcatcc tcggccaggt cgaagttgct cttgaagttg ggggtcaggc
8821 ccaggctcag ggcaatcagg tttccgaaca ggccattctt cttctcgccg ggcagctggg
8881 cgatcagatt ttccagcttt ctgctcttgc tcagtctggc agacaggatg gccttggcgt
8941 ccacgccgct ggcgttgatg gggttttcct cgaacagctg gttgtaggtc tgcaccagct
9001 ggatgaacag cttgtccacg tcgctgttgt cggggttcag gtcgccctcg atcaggaagt
9061 ggccccggaa cttgatcatg tgggccaggg ccagatagat cagccgcagg tcggccttgt
9121 cggtgctgtc caccagtttc tttctcaggt ggtagatggt ggggtacttc tcgtggtagg
9181 ccacctcgtc cacgatgttg ccgaagatgg ggtgccgctc gtgcttctta tcctcttcca
9241 ccaggaagga ctcttccagt ctgtggaaga agctgtcgtc caccttggcc atctcgttgc
9301 tgaagatctc ttgcagatag cagatccggt tcttccgtct ggtgtatctt cttctggcgg
9361 ttctcttcag ccgggtggcc tcggctgttt cgccgctgtc gaacagcagg gctccgatca
9421 ggttcttctt gatgctgtgc cggtcggtgt tgcccagcac cttgaatttc ttgctgggca
9481 ccttgtactc gtcggtgatc acggcccagc ccacagagtt ggtgccgatg tccaggccga
9541 tgctgtactt cttgtcgact ttccgcttct tctttggtga ctcgaactcg cttccgtcgg
9601 ctgtccgttt catggtggcg gctctcccta tagtgagtcg tattagcggc cgcggatctc
9661 tagcggatct gacggttcac taaaccagct ctgcttatat agacctccca ccgtacacgc
9721 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
9781 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
9841 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
9901 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
9961 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
10021 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
10081 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
10141 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
10201 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
10261 aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc
10321 ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc ttaaattttg
10381 ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg gcttaactat
10441 gcggcatcag agcagattgt actgagagta gaccctaggg gatcgggaga tcgatctccc
10501 gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
10561 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
10621 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
10681 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
10741 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
10801 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
10861 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
10921 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
10981 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
11041 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
11101 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
11161 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
11221 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
11281 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
11341 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
11401 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
11461 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
11521 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
11581 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
11641 gtgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
11701 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
11761 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
11821 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
11881 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
11941 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
12001 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
12061 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccg
//
