| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-CherryPicker1 |
| 质粒类型: | 慢病毒载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 8349 bp |
| 5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | CherryPicker1(C-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
慢病毒载体pLVX-CherryPicker1组成型表达CherryPicker融合蛋白； CherryPicker是mCherry与转铁蛋白受体膜锚定结构域融合蛋白。 |
| 产品目录号: | 632580 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
买家导航
pLVX-CherryPicker1载体质粒基本信息
pLVX-CherryPicker1质粒图谱载体图谱和pLVX-CherryPicker1载体序列质粒序列多克隆位点信息
pLVX-CherryPicker1质粒载体简介
pLVX-CherryPicker1质粒序列载体序列
LOCUS pLVX-CherryPicker1 8349 bp DNA circular 8-OCT-2010
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 632580,632574
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
misc_feature 2028..2151
/label=cPPT/CTS\(DNA\flap)
misc_feature 4318..4909
/label=WPRE
rep_origin complement(6217..6890)
/label=pUC\origin
CDS complement(7035..8031)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
LTR 5112..5748
/label=3'\LTR
misc_feature 3176..3684
/label=PGK\promoter
misc_feature 3705..4304
/label=Puro(R)
CDS 2236..3162
/label=CherryPicker
misc_feature 3163..3165
/label=Stop\codon
misc_feature 2206..2235
/label=MCS
BASE COUNT 2105 a 2140 c 2248 g 1856 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgaattctgc agtcgacggt accgcgggcc cgggatccgc
2221 ggccgcgttt aaacgatgat ggtggacggc gacaacagcc acgtggagat gaagctggcc
2281 gtggacgagg aagagaacgc cgacaacaac accaaggcca acgtgaccaa gcccaagcgg
2341 tgcagcggca gcatctgcta cggcacaatc gccgtgatcg tgttcttcct gatcggcttc
2401 atgatcggct acctgggcta ctgcaagtct agcgacggcc ctggcgagac aggcatggtg
2461 tccaagggcg aagaggacaa catggccatc atcaaagaat tcatgcggtt caaggtgcac
2521 atggaaggca gcgtgaacgg ccacgagttc gagatcgagg gcgagggcga aggcagacct
2581 tacgagggca cccagaccgc caagctgaaa gtgaccaagg gcggacccct gccctttgcc
2641 tgggacatcc tgagccccca gttcatgtac ggcagcaagg cctacgtgaa gcaccccgcc
2701 gacatccccg actacctgaa gctgagcttc cccgagggct tcaagtggga gagagtgatg
2761 aacttcgagg acggcggcgt ggtcaccgtg acccaggaca gcagcctgca ggacggcgag
2821 ttcatctaca aagtgaagct gcggggcacc aacttcccta gcgacggccc cgtgatgcag
2881 aaaaagacca tgggctggga ggccagcagc gagagaatgt accccgagga tggcgccctg
2941 aagggcgaga tcaagcagcg gctgaagctg aaggatggcg gccactacga cgccgaagtg
3001 aaaaccacct acaaggccaa gaaacccgtg cagctgcctg gcgcctacaa cgtgaacatc
3061 aagctggaca tcaccagcca caacgaggac tacaccatcg tggagcagta cgagagagcc
3121 gagggcagac acagcaccgg cggcatggac gagctgtaca aatgacaatc tagataattc
3181 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
3241 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
3301 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
3361 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
3421 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
3481 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
3541 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
3601 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
3661 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
3721 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
3781 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
3841 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
3901 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
3961 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
4021 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
4081 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
4141 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
4201 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
4261 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt ctggaacaat
4321 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
4381 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
4441 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
4501 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
4561 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
4621 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
4681 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
4741 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
4801 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
4861 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
4921 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
4981 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
5041 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
5101 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
5161 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
5221 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
5281 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
5341 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
5401 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
5461 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
5521 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
5581 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
5641 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
5701 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
5761 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
5821 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
5881 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
5941 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
6001 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
6061 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
6121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
6181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
6241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
6301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
6361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
6421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
6481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
6541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
6601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
6661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
6721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
6781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
6841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
6901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
6961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
7021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
7081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
7141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
7201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
7261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
7321 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
7381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
7441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
7501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
7561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
7621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
7681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
7741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
7801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
7861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
7921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
7981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
8041 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
8101 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
8161 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
8221 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
8281 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
8341 cttagtagt
//
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