| 质粒名称: | pScaf |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 111401 |
| 存储实验室: |
Shawn Douglas
|
|
载体骨架基本信息 |
|
| 载体名称: | pScaf |
| 载体出品公司: | Clontech |
| 空载体大小: | 3154bp |
| 完整质粒大小: | 4989 bp |
| 载体类型: | 细菌表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | TAAGGGATTTTGCCGATTTC |
| 3’测序引物 |
CACAGGAAACAGCTATGACCATGATT
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | pScaf was a gift from Shawn Douglas (Addgene plasmid # 111401 ; http://n2t.net/addgene:111401 ; RRID:Addgene_111401) |
| 参考文献部分: | Construction of a novel phagemid to produce custom DNA origami scaffolds. Nafisi PM, Aksel T, Douglas SM. Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub 2018 Aug 9. 10.1093/synbio/ysy015 PubMed 30984875 |
买家导航
pScaf载体质粒基本信息
pScaf质粒图谱载体图谱和pScaf载体序列质粒序列多克隆位点信息
pScaf质粒载体简介
pScaf质粒序列载体序列
LOCUS pScaf 3154 bp DNA circular SYN 25-AUG-2024
DEFINITION Phagemid for producing DNA origami scaffolds, derived from pUC18 and
M13mp18.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3154)
AUTHORS Nafisi PM, Aksel T, Douglas SM
TITLE Construction of a novel phagemid to produce custom DNA origami
scaffolds.
JOURNAL Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub
2018 Aug 9.
PUBMED 30984875
REFERENCE 2 (bases 1 to 3154)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol
(Oxf)."; date: "2018-01"; volume: "3(1). doi"; pages: "
10.1093/synbio/ysy015. Epub 2018 Aug 9"
FEATURES Location/Qualifiers
source 1..3154
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 6..25
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin 44..424
/direction=RIGHT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(788..804)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(788..804)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(801..823)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 812..828
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(836..866)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 881..902
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1019..1036)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1190..1778)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1270..1289)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1949..2809)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2572..2591
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2810..2914)
/gene="bla"
/label=AmpR promoter
primer_bind 2982..3000
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(3038..3060)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
ORIGIN
1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
421 tcggggtacc aaaaaaggat ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
481 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
541 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
601 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
661 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
721 gagtccacgt tctttaatag tggactcttg ttccaaactg gtttaacact cagaattcgt
781 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
841 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
901 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
961 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
1021 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
1081 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
1141 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
1201 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
1261 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
1321 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
1381 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
1441 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
1501 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
1561 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
1621 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
1681 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
1741 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
1801 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
1861 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
1921 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
1981 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
2041 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
2101 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
2161 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
2221 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
2281 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
2341 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
2401 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
2461 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
2521 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
2581 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
2641 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
2701 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
2761 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
2821 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
2881 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
2941 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
3001 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
3061 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
3121 cagcgggtgt tggcgggtgt cggggctggc ttaa
//
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