| 质粒名称: | pBiFC-VC155 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22011 |
| 存储实验室: | Chang-Deng Hu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV-HA |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 3836 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | VC155 |
| 物种来源: | 维多利亚水母 |
| 插入大小: | 252 bp |
| 基因突变: | Changed Alanine 206 to Lysine |
| 标签/融合蛋白: | HA（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | KpnI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | EBV-Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pBiFC-VC155 was a gift from Chang-Deng Hu (Addgene plasmid # 22011) |
| 参考文献部分: | Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques. 2006 Jan . 40(1):61-6. 10.2144/000112036 PubMed 16454041 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBiFC-VC155 4088 bp DNA SYN
DEFINITION pBiFC-VC155
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4088
/organism="pBiFC-VC155"
/mol_type="other DNA"
gene 139..384
/label="ECFP (468 - 713)"
/gene="ECFP (468 - 713)"
misc_feature 321..342
/label="EGFP_C_primer"
terminator complement(409..528)
/label="SV40_PA_terminator"
misc_feature complement(423..442)
/label="EBV_rev_primer"
promoter complement(640..658)
/label="M13_reverse_primer"
misc_feature complement(657..679)
/label="M13_pUC_rev_primer"
promoter complement(693..722)
/label="lac_promoter"
gene complement(1805..2665)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(1805..2665)
/label="ORF frame 2"
promoter complement(2707..2735)
/label="AmpR_promoter"
misc_feature complement(2894..2916)
/label="pGEX_3_primer"
misc_feature complement(3115..3258)
/label="lacZ_a"
misc_feature 3229..3251
/label="M13_pUC_fwd_primer"
promoter 3244..3260
/label="M13_forward20_primer"
misc_feature 3291..3578
/label="CAG_enhancer"
promoter 3296..3788
/label="CMV_immearly_promoter"
misc_feature 3745..3765
/label="CMV_fwd_primer"
promoter 3746..3815
/label="CMV_promoter"
promoter 3758..3877
/label="CMV2_promoter"
misc_feature 3789..3808
/label="pCEP_fwd_primer"
misc_feature 3791..3815
/label="LNCX_primer"
misc_feature 3891..3917
/label="pCMV_primer"
misc_feature 3932..3962
/label="SV40_late_19s_int"
misc_feature 3932..4028
/label="modSV40_late_16s_int"
CDS 4010..458
/label="ORF frame 2"
ORIGIN
1 ATGTACCCAT ACGATGTTCC AGATTACGCT CTTATGGCCA TGGAGGCCCG AATTCGGTCG
61 ACCGAGATCT CTCGAGGTAC CCGTCCGGCG TGCAAAATCC CGAACGACCT GAAACAGAAA
121 GTCATGAACC ACGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC
181 ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
241 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC AGTCCAAACT GAGCAAAGAC
301 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT
361 CTCGGCATGG ACGAGCTGTA CAAGTAAGCG GCCGCGGGGA TCCAGACATG ATAAGATACA
421 TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA
481 TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA
541 ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTCGGATC
601 CTCTAGAGTC GATCTGCAGG CATGCTAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG
661 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA
721 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG
781 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA
841 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG
901 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG
961 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC
1021 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
1081 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
1141 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
1201 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
1261 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
1321 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
1381 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
1441 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA
1501 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
1561 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
1621 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
1681 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
1741 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
1801 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
1861 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
1921 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA
1981 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
2041 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
2101 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
2161 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
2221 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
2281 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
2341 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
2401 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
2461 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
2521 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
2581 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
2641 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
2701 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
2761 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA
2821 TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG
2881 ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG
2941 CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG
3001 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG
3061 AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC
3121 TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA
3181 AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC
3241 GTTGTAAAAC GACGGCCAGT GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA
3301 CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA
3361 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT
3421 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA
3481 TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG
3541 ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT
3601 TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC
3661 ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT
3721 GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
3781 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT
3841 TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC
3901 TAGAGGAACT GAAAAACCAG AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC
3961 AGGTCCCGGA TCCGGTGGTG GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT
4021 ACTTCTAGGC CTGTACGGAA GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG
4081 GGCCCACC
//
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