质粒名称: | pGB411 |
---|---|
出品公司: | Addgene |
目录编号: | 134760 |
存储实验室: | Qi-Jun Chen |
载体骨架基本信息 |
|
载体名称: | pCambia |
完整质粒大小: | 20260 bp |
载体类型: | CRISPR |
载体抗性: | Ampicillin and Kanamycin |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
|
插入基因名称: | CRISPR/Cas9 |
物种来源: | Synthetic |
克隆方法: | 限制性内切酶 |
5’克隆位点: | 未知 |
文献引用方法 |
|
材料和方法部分: | pGB411 was a gift from Qi-Jun Chen (Addgene plasmid # 134760 ; http://n2t.net/addgene:134760 ; RRID:Addgene_134760) |
参考文献部分: | A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ. Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. 10.1104/pp.19.00767 PubMed 31558579 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGB411 20260 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION pCambia CRISPR/Cas9. ACCESSION . VERSION . KEYWORDS pGB411 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 20260) AUTHORS Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ TITLE A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. JOURNAL Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767. PUBMED 31558579 REFERENCE 2 (bases 1 to 20260) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..20260 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..25 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" primer_bind 213..235 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 227..244 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 228..244 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 311..690 /label=OsU3 promoter /note="Oryza sativa (rice) snRNA U3 promoter" promoter 713..817 /gene="bla" /label=AmpR promoter CDS 818..1678 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1036..1055) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" misc_RNA 1827..1902 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" promoter 1968..3960 /label=Ubi promoter /note="maize polyubiquitin gene promoter" CDS 3979..4044 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 4051..4071 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4096..8196 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" RBS 8109..8117 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 8197..8244 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" terminator 11517..11769 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(11794..11810) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(11794..11810) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(11807..11829) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 11818..11834 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(11842..11872) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11887..11908 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 12099..12775 /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" primer_bind 12712..12732 /label=35S promoter /note="CaMV 35S promoter, forward primer" CDS 12820..13371 /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTEI" polyA_signal 13378..13552 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" misc_feature 13630..13654 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 14118..14912 /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" terminator 14968..15054 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 15146..15173 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" rep_origin 15279..15867 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 15768..15787 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 16021..16038 /label=L4440 /note="L4440 vector, forward primer" misc_feature 16053..16193 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(16119..16138) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 16279..16301 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 16537..16731 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(16797..17870) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" CDS complement(18299..18928) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" ORIGIN 1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac 61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga 121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 241 cagtgccaag cttagtaatt catccaggtc accaagttct aggattttca gaactgcaac 301 ttattttatc aaggaatctt taaacatacg aacagatcac ttaaagttct tctgaagcaa 361 cttaaagtta tcaggcttgc atggatcttg gaggaatcag atgtgcagtc agggaccata 421 gcacaagaca ggcgtcttct actggtgcta ccagcaaatg ctggaagccg ggaacactgg 481 gtacgttgga aaccacgtga tgtgaagaag taagataaac tgtaggagaa aagcatttcg 541 tagtgggcca tgaagccttt caggacatgt attgcagtat gggccggccc attacgcaat 601 tggacgacaa caaagtctag tattagtacc acctcggcta tccacataga tcaaagctga 661 tttaaaagag ttgtgcagat gatccgtggc gtgagacctt cggggaaatg tgcgcggaac 721 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatgg gacaataacc 781 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 841 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 901 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 961 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 1021 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 1081 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 1141 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 1201 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 1261 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 1321 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 1381 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 1441 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 1501 tattgctgat aaatctggag ccggtgagcg tggctctcgc ggtatcattg cagcactggg 1561 gccagatggt aagccctccc gtatcgtagt tatctacacc acggggagtc aggcaactat 1621 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 1681 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 1741 aaggatctag gtgaagatcc tttttgataa tcccatgacc aaaatccctt aacgtgagtt 1801 ttcgttccac tgagcgtcag gtctcagttt tagagctaga aatagcaagt taaaataagg 1861 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttt ttgtccttct 1921 gtttttttag tcagtctctt ttttcagaag tacaacatct tgcatgcctg cagtgcagcg 1981 tgacccggtc gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat 2041 taccacatat tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat 2101 atttaaactt tactctacga ataatataat ctatagtact acaataatat cagtgtttta 2161 gagaatcata taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac 2221 aggactctac agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc 2281 ttcacctata taatacttca tccattttat tagtacatcc atttagggtt tagggttaat 2341 ggtttttata gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta 2401 agaaaactaa aactctattt tagttttttt atttaataat ttagatataa aatagaataa 2461 aataaagtga ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca 2521 tttttcttgt ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga gtctaacgga 2581 caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct 2641 ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca ccgttggact tgctccgctg 2701 tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag ccggcacggc aggcggcctc 2761 ctcctcctct cacggcaccg gcagctacgg gggattcctt tcccaccgct ccttcgcttt 2821 cccttcctcg cccgccgtaa taaatagaca ccccctccac accctctttc cccaacctcg 2881 tgttgttcgg agcgcacaca cacacaacca gatctccccc aaatccaccc gtcggcacct 2941 ccgcttcaag gtacgccgct cgtcctcccc ccccccccct ctctaccttc tctagatcgg 3001 cgttccggtc catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc 3061 gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac 3121 acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc 3181 gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg 3241 cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct 3301 tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag 3361 aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata 3421 catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac 3481 atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga 3541 tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca 3601 aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt 3661 tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt 3721 ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt 3781 acctatctat tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga 3841 tgatggcata tgcagcagct atatgtggat ttttttagcc ctgccttcat acgctattta 3901 tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag 3961 ccctaggcct actagatgga ttacaaggac cacgacgggg attacaagga ccacgacatt 4021 gattacaagg atgatgatga caagatggct ccgaagaaga agaggaaggt tggcatccac 4081 ggggtgccag ctgctgacaa gaagtactcg atcggcctcg atattgggac taactctgtt 4141 ggctgggccg tgatcaccga cgagtacaag gtgccctcaa agaagttcaa ggtcctgggc 4201 aacaccgatc ggcattccat caagaagaat ctcattggcg ctctcctgtt cgacagcggc 4261 gagacggctg aggctacgcg gctcaagcgc accgcccgca ggcggtacac gcgcaggaag 4321 aatcgcatct gctacctgca ggagattttc tccaacgaga tggcgaaggt tgacgattct 4381 ttcttccaca ggctggagga gtcattcctc gtggaggagg ataagaagca cgagcggcat 4441 ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg agaagtaccc tacgatctac 4501 catctgcgga agaagctcgt ggactccaca gataaggcgg acctccgcct gatctacctc 4561 gctctggccc acatgattaa gttcaggggc catttcctga tcgaggggga tctcaacccg 4621 gacaatagcg atgttgacaa gctgttcatc cagctcgtgc agacgtacaa ccagctcttc 4681 gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg ctatcctgtc cgctaggctc 4741 tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc cgggcgagaa gaagaacggc 4801 ctgttcggga atctcattgc gctcagcctg gggctcacgc ccaacttcaa gtcgaatttc 4861 gatctcgctg aggacgccaa gctgcagctc tccaaggaca catacgacga tgacctggat 4921 aacctcctgg cccagatcgg cgatcagtac gcggacctgt tcctcgctgc caagaatctg 4981 tcggacgcca tcctcctgtc tgatattctc agggtgaaca ccgagattac gaaggctccg 5041 ctctcagcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac cctcctgaag 5101 gcgctggtca ggcagcagct ccccgagaag tacaaggaga tcttcttcga tcagtcgaag 5161 aacggctacg ctgggtacat tgacggcggg gcctctcagg aggagttcta caagttcatc 5221 aagccgattc tggagaagat ggacggcacg gaggagctgc tggtgaagct caatcgcgag 5281 gacctcctga ggaagcagcg gacattcgat aacggcagca tcccacacca gattcatctc 5341 ggggagctgc acgctatcct gaggaggcag gaggacttct accctttcct caaggataac 5401 cgcgagaaga tcgagaagat tctgactttc aggatcccgt actacgtcgg cccactcgct 5461 aggggcaact cccgcttcgc ttggatgacc cgcaagtcag aggagacgat cacgccgtgg 5521 aacttcgagg aggtggtcga caagggcgct agcgctcagt cgttcatcga gaggatgacg 5581 aatttcgaca agaacctgcc aaatgagaag gtgctcccta agcactcgct cctgtacgag 5641 tacttcacag tctacaacga gctgactaag gtgaagtatg tgaccgaggg catgaggaag 5701 ccggctttcc tgtctgggga gcagaagaag gccatcgtgg acctcctgtt caagaccaac 5761 cggaaggtca cggttaagca gctcaaggag gactacttca agaagattga gtgcttcgat 5821 tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct ccctggggac ctaccacgat 5881 ctcctgaaga tcattaagga taaggacttc ctggacaacg aggagaatga ggatatcctc 5941 gaggacattg tgctgacact cactctgttc gaggaccggg agatgatcga ggagcgcctg 6001 aagacttacg cccatctctt cgatgacaag gtcatgaagc agctcaagag gaggaggtac 6061 accggctggg ggaggctgag caggaagctc atcaacggca ttcgggacaa gcagtccggg 6121 aagacgatcc tcgacttcct gaagagcgat ggcttcgcga accgcaattt catgcagctg 6181 attcacgatg acagcctcac attcaaggag gatatccaga aggctcaggt gagcggccag 6241 ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cgccagctat caagaagggg 6301 attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg tcatgggcag gcacaagcct 6361 gagaacatcg tcattgagat ggcccgggag aatcagacca cgcagaaggg ccagaagaac 6421 tcacgcgaga ggatgaagag gatcgaggag ggcattaagg agctggggtc ccagatcctc 6481 aaggagcacc cggtggagaa cacgcagctg cagaatgaga agctctacct gtactacctc 6541 cagaatggcc gcgatatgta tgtggaccag gagctggata ttaacaggct cagcgattac 6601 gacgtcgatc atatcgttcc acagtcattc ctgaaggatg actccattga caacaaggtc 6661 ctcaccaggt cggacaagaa ccggggcaag tctgataatg ttccttcaga ggaggtcgtt 6721 aagaagatga agaactactg gcgccagctc ctgaatgcca agctgatcac gcagcggaag 6781 ttcgataacc tcacaaaggc tgagaggggc gggctctctg agctggacaa ggcgggcttc 6841 atcaagaggc agctggtcga gacacggcag atcactaagc acgttgcgca gattctcgac 6901 tcacggatga acactaagta cgatgagaat gacaagctga tccgcgaggt gaaggtcatc 6961 accctgaagt caaagctcgt ctccgacttc aggaaggatt tccagttcta caaggttcgg 7021 gagatcaaca attaccacca tgcccatgac gcgtacctga acgcggtggt cggcacagct 7081 ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt acggggacta caaggtttac 7141 gatgtgagga agatgatcgc caagtcggag caggagattg gcaaggctac cgccaagtac 7201 ttcttctact ctaacattat gaatttcttc aagacagaga tcactctggc caatggcgag 7261 atccggaagc gccccctcat cgagacgaac ggcgagacgg gggagatcgt gtgggacaag 7321 ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc cacaagtgaa tatcgtcaag 7381 aagacagagg tccagactgg cgggttctct aaggagtcaa ttctgcctaa gcggaacagc 7441 gacaagctca tcgcccgcaa gaaggactgg gatccgaaga agtacggcgg gttcgacagc 7501 cccactgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa gtccaagaag 7561 ctcaagagcg tgaaggagct gctggggatc acgattatgg agcgctccag cttcgagaag 7621 aacccgatcg atttcctgga ggcgaagggc tacaaggagg tgaagaagga cctgatcatt 7681 aagctcccca agtactcact cttcgagctg gagaacggca ggaagcggat gctggcttcc 7741 gctggcgagc tgcagaaggg gaacgagctg gctctgccgt ccaagtatgt gaacttcctc 7801 tacctggcct cccactacga gaagctcaag ggcagccccg aggacaacga gcagaagcag 7861 ctgttcgtcg agcagcacaa gcattacctc gacgagatca ttgagcagat ttccgagttc 7921 tccaagcgcg tgatcctggc cgacgcgaat ctggataagg tcctctccgc gtacaacaag 7981 caccgcgaca agccaatcag ggagcaggct gagaatatca ttcatctctt caccctgacg 8041 aacctcggcg cccctgctgc tttcaagtac ttcgacacaa ctatcgatcg caagaggtac 8101 acaagcacta aggaggtcct ggacgcgacc ctcatccacc agtcgattac cggcctctac 8161 gagacgcgca tcgacctgtc tcagctcggg ggcgacaagc ggccagcggc gacgaagaag 8221 gcggggcagg cgaagaagaa gaagtgagct cagagctttc gttcgtatca tcggtttcga 8281 caacgttcgt caagttcaat gcatcagttt cattgcgcac acaccagaat cctactgagt 8341 ttgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt 8401 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag 8461 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt 8521 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa 8581 aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acacttgtag 8641 ttgtaccatt atgcttattc actaggcaac aaatatattt tcagacctag aaaagctgca 8701 aatgttactg aatacaagta tgtcctcttg tgttttagac atttatgaac tttcctttat 8761 gtaattttcc agaatccttg tcagattcta atcattgctt tataattata gttatactca 8821 tggatttgta gttgagtatg aaaatatttt ttaatgcatt ttatgacttg ccaattgatt 8881 gacaacgaat tcggatcctc catgcctaca tcaactaatt tgattccttg agtttacgtt 8941 tagtgatatg tctattttta gagcttgttg gggcttcggc ctcagctcta gccagccaaa 9001 catgttctac caagtaccct atgttggcat gatatagtga tgcattataa caataaatga 9061 gcgagggatt gctggctgaa aaagctatac tagctgcatt tggttatagt taaccgaact 9121 attaattgcg tgtacaacaa aataaaaaaa atgcatgttg cacattcttt cattaacatt 9181 atgttttggt agtgtgaatt agaaatttga ttgacagtag atcgacaaac atagtttcaa 9241 tatgcttaag ttagttatga ctttaacata tcagtctcct tgatattttc gttttagatt 9301 cgtctctctt ctagtgtgta tgtccacctt ccatagcagt gagggttcca ttccatccct 9361 gtaaaaaaaa atcaaccact actatttatt tctaaaagca aaatgataaa atatcatttt 9421 tttaatagaa taagaaagta ttgggtacat agtgatgtgc acgtaggagt aaacataaaa 9481 aaggcagatt ttctaggttg gcccaggtgt gcagtgcagc aaattattcc gcgttggcac 9541 ggccgcagcg cagagacagc ctcaagctct caactttcca gtgaggccgg cgcctcttta 9601 catctcattc ccccaatttc ccaccaccat cgccattgcc accacctctc ctatatctcg 9661 ccctccccct cctccctccc acgccattcg cctccttctt gctgcagccg ccatccccgg 9721 ttcggttctc tcctcttctt taggtgagca actgcctctc catgtccagg ccctcccggc 9781 agggaagact cgtgtttttg taggatgaat gtttagttca agttacattt ttctgtatta 9841 atctatagtc tttgtaaaca ctgttttgaa tgatttattt tgtgttatgc agatcagtta 9901 ctagatggcg caagttagca gaatctgcaa tggtgtgcag aacccatctc ttatctccaa 9961 tctctcgaaa tccagtcaac gcaaatctcc cttatcggtt tctctgaaga cgcagcagca 10021 tccacgagct tatccgattt cgtcgtcgtg gggattgaag aagagtggga tgacgttaat 10081 tggctctgag cttcgtcctc ttaaggtcat gtcttctgtt tccacggcgt gcatgtcgca 10141 cggtgcaagc agccggcccg caaccgcccg caaatcctct ggcctttccg gaaccgtccg 10201 cattcccggc gacaagtcga tctcccaccg gtccttcatg ttcggcggcc tcgcgagcgg 10261 tgaaacgcgc atcaccggcc ttctggaagg cgaggacgtc atcaatacgg gcaaggcgat 10321 gcaggccatg ggcgccagga tccgtaagga aggcgacacc tggatcatcg atggcgtcgg 10381 caatggcggc ctcctggcgc ctgaggcgcc gctcgatttc ggcaatgccg ccacgggctg 10441 ccgcctgacc atgggcctcg tcggggtcta cgatttcgac agcaccttca tcggcgacgc 10501 ctcgctcaca aagcgcccga tgggccgcgt gttgaacccg ctgcgcgaaa tgggcgtgca 10561 ggtcaaatcg gaagacggtg accgtcttcc cgttaccttg cgcgggccga agacgccgac 10621 gccgatcacc taccgcgtgc cgatggcctc cgcacaggtg aagtccgccg tgctgctcgc 10681 cggcctcaac acgcccggca tcacgacggt catcgagccg atcatgacgc gcgatcatac 10741 ggaaaagatg ctgcagggct ttggcgccaa ccttaccgtc gagacggatg cggacggcgt 10801 gcgcaccatc cgcctggaag gtcgcggcaa gctcaccggc caagtcatcg acgtgccggg 10861 cgacccgtcc tcgacggcct tcccgctggt tgcggccctg cttgttccgg gctccgacgt 10921 caccatcctc aacgtgctga tgaaccccac ccgcaccggc ctcatcctga cgctgcagga 10981 aatgggcgcc gacatcgaag tcatcaaccc gcgccttgcc ggcggcgaag acgtggcgga 11041 cctgcgcgtt cgctcctcca cgctgaaggg cgtcacggtg ccggaagacc gcgcgccttc 11101 gatgatcgac gaatatccga ttctcgctgt cgccgccgcc ttcgcggaag gggcgaccgt 11161 gatgaacggt ctggaagaac tccgcgtcaa ggaaagcgac cgcctctcgg ccgtcgccaa 11221 tggcctcaag ctcaatggcg tggattgcga tgagggcgag acgtcgctcg tcgtgcgcgg 11281 ccgccctgac ggcaaggggc tcggcaacgc ctcgggcgcc gccgtcgcca cccatctcga 11341 tcaccgcatc gccatgagct tcctcgtcat gggcctcgtg tcggaaaacc ctgtcacggt 11401 ggacgatgcc acgatgatcg ccacgagctt cccggagttc atggacctga tggccgggct 11461 gggcgcgaag atcgaactct ccgatacgaa ggctgcctga ctcgacgaat ttccccgatc 11521 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga 11581 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga 11641 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga 11701 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt 11761 tactagatcg ggaactaggt aattcgtaat catgtcatag ctgtttcctg tgtgaaattg 11821 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 11881 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 11941 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 12001 gcgtattggc tagagcagct tgccaacatg gtggagcacg acactctcgt ctactccaag 12061 aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta 12121 atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca 12181 gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt 12241 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg 12301 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga 12361 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc 12421 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc 12481 tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca 12541 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg 12601 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca 12661 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc 12721 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca 12781 ccagtctctc tctacaaatc tatctctctc gagtctacca tgagcccaga acgacgcccg 12841 gccgacatcc gccgtgccac cgaggcggac atgccggcgg tctgcaccat cgtcaaccac 12901 tacatcgaga caagcacggt caacttccgt accgagccgc aggaaccgca ggagtggacg 12961 gacgacctcg tccgtctgcg ggagcgctat ccctggctcg tcgccgaggt ggacggcgag 13021 gtcgccggca tcgcctacgc gggcccctgg aaggcacgca acgcctacga ctggacggcc 13081 gagtcgaccg tgtacgtctc cccccgccac cagcggacgg gactgggctc cacgctctac 13141 acccacctcc tgaagtccct ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg 13201 ctgcccaacg acccgagcgt gcgcatgcac gaggcgctcg gatatgcccc acgcggcatg 13261 ctgcgggcgg ccggcttcaa gcacgggaac tggcatgacg tgggtttctg gcagctggac 13321 ttcagcctgc cggtaccgcc ccgtccggtc ctgcccgtca ccgagatttg actcgagttt 13381 ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg 13441 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta 13501 tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat 13561 taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag 13621 cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg 13681 gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag 13741 cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac 13801 ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg 13861 taacgatgac agagcgttgc tgcctgtgat caccgcggct ggcaaacagc tattatgggt 13921 attatgggtc tagtgggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt 13981 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt 14041 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa 14101 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg 14161 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg 14221 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct 14281 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa 14341 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg 14401 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt 14461 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata 14521 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg 14581 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt 14641 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca 14701 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg 14761 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag 14821 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa 14881 aatattatat tttactggat gaattgtttt agtacctaat ctcggggacg tctaactact 14941 aagcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc ggaagactgg 15001 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg 15061 ggagcggatt tgaacgttgt gaagcaacgg cccggagggt ggcgggcagg acgcccgcca 15121 taaactgcca ggcatcaaac taagcagaag gccatcctga cggatggcct ttttgcgttt 15181 ctacaaactc ttcctgttag atgcatgacc aaaatccctt aacgtgagtt ttcgttccac 15241 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 15301 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 15361 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 15421 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 15481 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 15541 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 15601 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 15661 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 15721 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 15781 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 15841 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 15901 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 15961 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 16021 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat 16081 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca 16141 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac 16201 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 16261 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 16321 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct 16381 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc 16441 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct 16501 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta 16561 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata 16621 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg 16681 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaacaga 16741 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag 16801 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca 16861 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg 16921 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc 16981 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa 17041 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc 17101 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc 17161 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc 17221 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt 17281 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag 17341 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct 17401 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa 17461 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc 17521 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg 17581 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct 17641 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc 17701 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct 17761 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca 17821 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct 17881 cgggcttggg ggttccagtg ccattgcagg gccggcaggc aacccagccg cttacgcctg 17941 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt 18001 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc 18061 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg 18121 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc 18181 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc 18241 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc 18301 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct 18361 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct 18421 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac 18481 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat 18541 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt 18601 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca 18661 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca 18721 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca 18781 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc 18841 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt 18901 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca 18961 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct 19021 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt 19081 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg 19141 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg 19201 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg 19261 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc 19321 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg 19381 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg 19441 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg 19501 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg 19561 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct 19621 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg 19681 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg 19741 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa 19801 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg 19861 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa 19921 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa 19981 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg 20041 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt 20101 tggatcaaag tacccataat acccataata gctgtttgcc agtactttga tcccgagggg 20161 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 20221 taaatatccg attattctaa taaacgctct tttctcttag //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:pG3K-U6SC
- 下一篇:没有了