| 载体名称: | pNF-kB-Luc |
|---|---|
| 质粒类型: | 哺乳动物细胞载体；信号通路报告载体 |
| 终止子: | SV40 poly(A) signal |
| 复制子: | pUC |
| 启动子: | SV40 |
| 载体大小: | 5.7 kb |
| 5' 测序引物及序列: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 哺乳细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 产品目录号: | -- |
买家导航
pNFkB-Luc载体基本信息
pNFkB-Luc载体图谱质粒图谱和多克隆位点信息
pNFkB-Luc载体简介
pNFkB-Luc载体序列
LOCUS pNF-KB-Luc 5695 bp DNA circular SYN 26-NOV-2020
SOURCE synthetic DNA construct.
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5695)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, Nov 26, 2020 from SnapGene Viewer 5.1.4.1 to
Vector NTI(R) format
http://www.biofeng.com
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|931219200|
COMMENT VNTDBDATE|-13623264|
COMMENT LSOWNER|
COMMENT VNTNAME|pNF-KB-Luc|
COMMENT VNTAUTHORNAME|Biofeng.com|
COMMENT VNTAUTHOREML|order@biofeng.com|
COMMENT VNTOAUTHORNAME|UNKNOWN|
FEATURES Location/Qualifiers
promoter 32..136
/gene="bla"
/vntifkey="30"
/label=AmpR\promoter
CDS 137..997
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/vntifkey="4"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
misc_feature 1934..2074
/vntifkey="21"
/label=bom
/note="basis of mobility region from pBR322"
polyA_signal 2539..2671
/vntifkey="25"
/label=SV40\poly(A)\signal
/note="SV40 polyadenylation signal"
promoter 2757..2788
/vntifkey="30"
/label=minP
/note="minimal TATA-box promoter with low basal activity"
CDS 2815..4467
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/vntifkey="4"
/label=luciferase
intron 4598..4663
/vntifkey="15"
/label=small\t\intron
/note="SV40 (simian virus 40) small t antigen intron"
polyA_signal 5233..5367
/vntifkey="25"
/label=SV40\poly(A)\signal
/note="SV40 polyadenylation signal"
misc_feature 2679..2748
/vntifkey="21"
/label=NF-KB(5x)
/note="NF-KB(5x) ;GGGGACTTTCCGC"
BASE COUNT 1580 a 1257 c 1278 g 1580 t
ORIGIN
1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttcact gcattctagt
2401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcagcca tatcacattt
2461 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
2521 atgaatgcaa ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
2581 agcatcacaa atttcacaaa taaagcaatt tttcactgca ttctattgtg gtttgtccaa
2641 actcatcaat gtatcttatc atgtctggat ccaagctagg ggactttccg cttggggact
2701 ttccgctggg gactttccgc tggggacttt ccgctgggga ctttccgcgg tgactctaga
2761 gggtatataa tggaagctcg aattccagct tggcattccg gtactgttgg taaaatggaa
2821 gacgccaaaa acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct
2881 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
2941 acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg
3001 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
3061 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
3121 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag
3181 cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa
3241 ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag
3301 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta
3361 ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact
3421 gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc
3481 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
3541 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
3601 gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt
3661 caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac
3721 aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa
3781 gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc
3841 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
3901 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
3961 ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta
4021 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
4081 atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt
4141 aaatacaaag gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc
4201 aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc
4261 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
4321 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
4381 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
4441 aagaagggcg gaaagtccaa attgtaaaat gtaactgtat tcagcgatga cgaaattctt
4501 agctattgta atactctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata
4561 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
4621 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
4681 ttatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
4741 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc tcaaaagaag
4801 agaaaggtag agacccaagg actttccttc agaattgcta agttttttga gtcatgctgt
4861 gtttagtaat agaactcttg cttgctttgc tatttacaac cacaaaggaa aaagctgcac
4921 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
4981 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
5041 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
5101 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
5161 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
5221 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
5281 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
5341 atcaatgtat cttatcatgt ctggatcccc aggaagctcc tctgtgtcct cataaaccct
5401 aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc tctgtgtcct
5461 cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca cagaccgctt
5521 tctaaggggt aattttaaaa tatctgggaa gtcccttcca ctgctgtgtt ccagaagtgt
5581 tggtaaacag cccacaaatg tcaacagcag aaacatacaa gctgtcactt tgcacaaagg
5641 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tctta
//
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