出品公司: | Agilent |
---|---|
载体名称: | pNF-kB-TA-Luc |
质粒类型: | 哺乳动物细胞载体;信号通路报告载体 |
终止子: | SV40 poly(A) signal |
复制子: | pUC |
启动子: | TK |
载体大小: | 4.9kb |
5' 测序引物及序列: | Sv40-polyA-R:GAAATTTGTGATGCTATTGC |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | DH5α 等 |
宿主细胞(系): | -- |
备注: | -- |
产品目录号: | -- |
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pNF-kB-TA-Luc载体质粒基本信息
pNF-kB-TA-Luc质粒图谱载体图谱和pNF-kB-TA-Luc载体序列质粒序列多克隆位点信息
pNF-kB-TA-Luc质粒载体简介
pNF-kB-TA-Luc质粒序列载体序列
LOCUS pNF-kB-TA-Luc 4902 bp ds-DNA circular SYN 13-MAR-2018 DEFINITION synthetic circular DNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4902) FEATURES Location/Qualifiers source 1..4902 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 34..73 /label=NFkB promoter 86..117 /label=minP /note="minimal TATA-box promoter with low basal activity" regulatory 173..182 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 179..1831 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" polyA_signal 1872..1993 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(2412..3000) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3171..4031) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4032..4136) /gene="bla" /label=AmpR promoter rep_origin 4163..4618 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" polyA_signal 4749..4797 /note="synthetic polyadenylation signal" misc_feature 4811..4902 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" BASE COUNT 1275 a 1159 c 1181 g 1286 t ORIGIN 1 atcgataggt accgagctct tacgcgtgct agcgggaatt tccgggaatt tccgggaatt 61 tccgggaatt tccagatcta gactctagag ggtatataat ggaagctcga attccagctt 121 ggcattccgg tactgttggt aaaaagcttg gcattccggt actgttggta aagccaccat 181 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac 241 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc 301 ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt 361 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg 421 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc 481 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc 541 gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa 601 aaagctccca atcatccaaa aaattattat catggattct aaaacggatt accagggatt 661 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt 721 tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc 781 tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca 841 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt 901 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg 961 agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa 1021 gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat 1081 tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa 1141 ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg 1201 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc 1261 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac 1321 gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta 1381 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg 1441 agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct 1501 gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca 1561 ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc 1621 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta 1681 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga 1741 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa 1801 ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc ggccgcttcg 1861 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa 1921 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg 1981 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt 2041 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc 2101 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca 2161 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc 2221 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 2281 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 2341 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 2401 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 2461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 2521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 2581 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 2641 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 2701 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 2761 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 2821 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 2881 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 2941 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 3001 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 3061 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 3121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 3181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 3241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 3301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 3361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 3421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 3481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 3541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 3601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 3661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 3721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 3781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 3841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 3901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 3961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 4021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 4081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 4141 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 4201 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 4261 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 4321 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 4381 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 4441 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 4501 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 4561 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttgc 4621 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 4681 ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga ggtacttgga 4741 gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat 4801 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat 4861 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct //
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