| 质粒名称: | pnCas9-PBE |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 98164 |
| 存储实验室: | Caixia Gao |
|
载体骨架基本信息 |
|
| 载体名称: | pJIT163-Ubi-GFP |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | NLS-APOBEC1-XTEN-nCas9-UGI-NLS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | Ubi |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | BamHI(未破坏) |
| 3' 克隆位点: | Bsp1407I(未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pnCas9-PBE was a gift from Caixia Gao (Addgene plasmid # 98164 ; http://n2t.net/addgene:98164 ; RRID:Addgene_98164) |
| 参考文献部分: | Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. 10.1038/nbt.3811 PubMed 28244994 |
买家导航
pnCas9PBE载体基本信息
pnCas9PBE载体图谱质粒图谱和多克隆位点信息
pnCas9PBE载体简介
pnCas9PBE载体序列
LOCUS pnCas9-PBE 10202 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses CRISPR base editor in protoplasts.
ACCESSION .
VERSION .
KEYWORDS pnCas9-PBE
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10202)
AUTHORS Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C
TITLE Precise base editing in rice, wheat and maize with a Cas9-cytidine
deaminase fusion.
JOURNAL Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub
2017 Feb 27.
PUBMED 28244994
REFERENCE 2 (bases 1 to 10202)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10202
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 2034..2054
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2055..2738
/codon_start=1
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/label=APOBEC-1
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
WATGLK"
CDS 2790..6890
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6891..6938
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 6960..7208
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 7221..7241
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 7315..7491
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind complement(8045..8061)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8045..8061)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8058..8080)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8069..8085
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8093..8123)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8138..8159
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8276..8293)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8447..9035)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8527..8546)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9206..10066)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9829..9848
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10067..10171)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gccaagctaa ttcgagctcg gtacctgacc cggtcgtgcc cctctctaga gataatgagc
61 attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt
121 gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata
181 gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta
241 aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt
301 gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta
361 catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt
421 ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta
481 ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta
541 agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt
601 aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc
661 aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc gatcgagagt
721 tccgctccac cgttggactt gctccgctgt cggcatccag aaattgcgtg gcggagcggc
781 agacgtgagc cggcacggca ggcggcctcc tcctcctctc acggcaccgg cagctacggg
841 ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagacac
901 cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac acacaaccag
961 atctccccca aatccacccg tcggcacctc cgcttcaagg tacgccgctc gtcctccccc
1021 cccccccctc tctaccttct ctagatcggc gttccggtcc atggttaggg cccggtagtt
1081 ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt
1141 cgtacacgga tgcgacctgt acgtcagaca cgttctgatt gctaacttgc cagtgtttct
1201 ctttggggaa tcctgggatg gctctagccg ttccgcagac gggatcgatt tcatgatttt
1261 ttttgtttcg ttgcataggg tttggtttgc ccttttcctt tatttcaata tatgccgtgc
1321 acttgtttgt cgggtcatct tttcatgctt ttttttgtct tggttgtgat gatgtggtct
1381 ggttgggcgg tcgttctaga tcggagtaga attaattctg tttcaaacta cctggtggat
1441 ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga attgaagatg
1501 atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt actgatgcat
1561 atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc
1621 attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt attaattttg
1681 gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga tggaaatatc
1741 gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca tgatggcata
1801 tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata aacaagtatg
1861 ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca gctatatgtg
1921 gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt cttttgtcga
1981 tgctcaccct gttgtttggt gttacttctg caaagcttgt cgacggatcc atgccaaaga
2041 agaagaggaa ggtttcatcg gagaccggcc ctgttgctgt tgaccccacc ctgcggcgga
2101 gaatcgagcc acacgagttc gaggtgttct tcgacccaag ggagctccgc aaggagacgt
2161 gcctcctgta cgagatcaac tggggcggca ggcactccat ctggaggcac accagccaaa
2221 acaccaacaa gcacgtggag gtcaacttca tcgagaagtt caccaccgag aggtacttct
2281 gcccaaacac ccgctgctcc atcacctggt tcctgtcctg gagcccatgc ggcgagtgct
2341 ccagggccat caccgagttc ctcagccgct acccacacgt caccctgttc atctacatcg
2401 ccaggctcta ccaccacgcc gacccaagga acaggcaggg cctccgcgac ctgatctcca
2461 gcggcgtgac catccaaatc atgaccgagc aggagtccgg ctactgctgg aggaacttcg
2521 tcaactactc cccaagcaac gaggcccact ggccaaggta cccacacctc tgggtgcgcc
2581 tctacgtgct cgagctgtac tgcatcatcc tcggcctgcc accatgcctc aacatcctga
2641 ggcgcaagca accacagctg accttcttca ccatcgccct ccaaagctgc cactaccaga
2701 ggctcccacc acacatcctg tgggctaccg gcctcaagtc cggcagcgag acgccaggca
2761 cctccgagag cgctacgcct gaacttaagg acaagaagta ctcgatcggc ctcgccatcg
2821 ggacgaactc agttggctgg gccgtgatca ccgacgagta caaggtgccc tctaagaagt
2881 tcaaggtcct ggggaacacc gaccgccatt ccatcaagaa gaacctcatc ggcgctctcc
2941 tgttcgacag cggggagacc gctgaggcta cgaggctcaa gagaaccgct aggcgccggt
3001 acacgagaag gaagaacagg atctgctacc tccaagagat tttctccaac gagatggcca
3061 aggttgacga ttcattcttc caccgcctgg aggagtcttt cctcgtggag gaggataaga
3121 agcacgagcg gcatcccatc ttcggcaaca tcgtggacga ggttgcctac cacgagaagt
3181 accctacgat ctaccatctg cggaagaagc tcgtggactc caccgataag gcggacctca
3241 gactgatcta cctcgctctg gcccacatga tcaagttccg cggccatttc ctgatcgagg
3301 gggatctcaa cccagacaac agcgatgttg acaagctgtt catccaactc gtgcagacct
3361 acaaccaact cttcgaggag aacccgatca acgcctctgg cgtggacgcg aaggctatcc
3421 tgtccgcgag gctctcgaag tccaggaggc tggagaacct gatcgctcag ctcccaggcg
3481 agaagaagaa cggcctgttc gggaacctca tcgctctcag cctggggctc accccgaact
3541 tcaagtcgaa cttcgatctc gctgaggacg ccaagctgca actctccaag gacacctacg
3601 acgatgacct cgataacctc ctggcccaga tcggcgatca atacgcggac ctgttcctcg
3661 ctgccaagaa cctgtcggac gccatcctcc tgtcagatat cctccgcgtg aacaccgaga
3721 tcacgaaggc tccactctct gcctccatga tcaagcgcta cgacgagcac catcaggatc
3781 tgaccctcct gaaggcgctg gtccgccaac agctcccgga gaagtacaag gagattttct
3841 tcgatcagtc gaagaacggc tacgctgggt acatcgacgg cggggcctca caagaggagt
3901 tctacaagtt catcaagcca atcctggaga agatggacgg cacggaggag ctcctggtga
3961 agctcaacag ggaggacctc ctgcggaagc agagaacctt cgataacggc agcatccccc
4021 accaaatcca tctcggggag ctgcacgcca tcctgagaag gcaagaggac ttctaccctt
4081 tcctcaagga taaccgggag aagatcgaga agatcctgac cttcagaatc ccatactacg
4141 tcggccctct cgcgcggggg aactcaagat tcgcttggat gacccgcaag tctgaggaga
4201 ccatcacgcc gtggaacttc gaggaggtgg tggacaaggg cgctagcgct cagtcgttca
4261 tcgagaggat gaccaacttc gacaagaacc tgcccaacga gaaggtgctc cctaagcact
4321 cgctcctgta cgagtacttc accgtctaca acgagctcac gaaggtgaag tacgtcaccg
4381 agggcatgcg caagccagcg ttcctgtccg gggagcagaa gaaggctatc gtggacctcc
4441 tgttcaagac caaccggaag gtcacggtta agcaactcaa ggaggactac ttcaagaaga
4501 tcgagtgctt cgattcggtc gagatcagcg gcgttgagga ccgcttcaac gccagcctcg
4561 ggacctacca cgatctcctg aagatcatca aggataagga cttcctggac aacgaggaga
4621 acgaggatat cctggaggac atcgtgctga ccctcacgct gttcgaggac agggagatga
4681 tcgaggagcg cctgaagacg tacgcccatc tcttcgatga caaggtcatg aagcaactca
4741 agcgccggag atacaccggc tgggggaggc tgtcccgcaa gctcatcaac ggcatccggg
4801 acaagcagtc cgggaagacc atcctcgact tcctcaagag cgatggcttc gccaacagga
4861 acttcatgca actgatccac gatgacagcc tcaccttcaa ggaggatatc caaaaggctc
4921 aagtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct ggctcccccg
4981 cgatcaagaa gggcatcctc cagaccgtga aggttgtgga cgagctcgtg aaggtcatgg
5041 gccggcacaa gcctgagaac atcgtcatcg agatggccag agagaaccaa accacgcaga
5101 aggggcaaaa gaactctagg gagcgcatga agcgcatcga ggagggcatc aaggagctgg
5161 ggtcccaaat cctcaaggag cacccagtgg agaacaccca actgcagaac gagaagctct
5221 acctgtacta cctccagaac ggcagggata tgtacgtgga ccaagagctg gatatcaacc
5281 gcctcagcga ttacgacgtc gatcatatcg ttccccagtc tttcctgaag gatgactcca
5341 tcgacaacaa ggtcctcacc aggtcggaca agaaccgcgg caagtcagat aacgttccat
5401 ctgaggaggt cgttaagaag atgaagaact actggaggca gctcctgaac gccaagctga
5461 tcacgcaaag gaagttcgac aacctcacca aggctgagag aggcgggctc tcagagctgg
5521 acaaggccgg cttcatcaag cggcagctgg tcgagaccag acaaatcacg aagcacgttg
5581 cgcaaatcct cgactctcgg atgaacacga agtacgatga gaacgacaag ctgatcaggg
5641 aggttaaggt gatcaccctg aagtctaagc tcgtctccga cttcaggaag gatttccagt
5701 tctacaaggt tcgcgagatc aacaactacc accatgccca tgacgcttac ctcaacgctg
5761 tggtcggcac cgctctgatc aagaagtacc caaagctgga gtccgagttc gtgtacgggg
5821 actacaaggt ttacgatgtg cgcaagatga tcgccaagtc ggagcaagag atcggcaagg
5881 ctaccgccaa gtacttcttc tactcaaaca tcatgaactt cttcaagacc gagatcacgc
5941 tggccaacgg cgagatccgg aagagaccgc tcatcgagac caacggcgag acgggggaga
6001 tcgtgtggga caagggcagg gatttcgcga ccgtccgcaa ggttctctcc atgccccagg
6061 tgaacatcgt caagaagacc gaggtccaaa cgggcgggtt ctcaaaggag tctatcctgc
6121 ctaagcggaa cagcgacaag ctcatcgcca gaaagaagga ctgggaccca aagaagtacg
6181 gcgggttcga cagccctacc gtggcctact cggtcctggt tgtggcgaag gttgagaagg
6241 gcaagtccaa gaagctcaag agcgtgaagg agctcctggg gatcaccatc atggagaggt
6301 ccagcttcga gaagaaccca atcgacttcc tggaggccaa gggctacaag gaggtgaaga
6361 aggacctgat catcaagctc ccgaagtact ctctcttcga gctggagaac ggcaggaaga
6421 gaatgctggc ttccgctggc gagctccaga aggggaacga gctcgcgctg ccaagcaagt
6481 acgtgaactt cctctacctg gcttcccact acgagaagct caagggcagc ccggaggaca
6541 acgagcaaaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag atcatcgagc
6601 aaatctccga gttcagcaag cgcgtgatcc tcgccgacgc gaacctggat aaggtcctct
6661 ccgcctacaa caagcaccgg gacaagccca tcagagagca agcggagaac atcatccatc
6721 tcttcaccct gacgaacctc ggcgctcctg ctgctttcaa gtacttcgac accacgatcg
6781 atcggaagag atacacctcc acgaaggagg tcctggacgc gaccctcatc caccagtcga
6841 tcaccggcct gtacgagacg aggatcgacc tctcacaact cggcggggat aagagacccg
6901 cagcaaccaa gaaggcaggg caagcaaaga agaagaagac gcgtgactcc ggcggcagca
6961 ccaacctgtc cgacatcatc gagaaggaga cgggcaagca actcgtgatc caggagagca
7021 tcctcatgct gccagaggag gtggaggagg tcatcggcaa caagccagag tccgacatcc
7081 tggtgcacac cgcctacgac gagtccaccg acgagaacgt catgctcctg accagcgacg
7141 ccccagagta caagccatgg gccctcgtca tccaggacag caacggggag aacaagatca
7201 agatgctgtc gggggggagc ccaaagaaga agcggaaggt gtagtgtaca agtaacccgg
7261 gaattcggta cgctgaaatc accagtctct ctctacaaat ctatctctct ctattttctc
7321 cataaataat gtgtgagtag tttcccgata agggaaatta gggttcttat agggtttcgc
7381 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
7441 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat ctcctaaagt
7501 ccctatagat ctttgtcgtg aatataaacc agacacgaga cgactaaacc tggagcccag
7561 acgccgttcg aagctagaag taccgcttag gcaggaggcc gttagggaaa agatgctaag
7621 gcagggttgg ttacgttgac tcccccgtag gtttggttta aatatgatga agtggacgga
7681 aggaaggagg aagacaagga aggataaggt tgcaggccct gtgcaaggta agaagatgga
7741 aatttgatag aggtacgcta ctatacttat actatacgct aagggaatgc ttgtatttat
7801 accctatacc ccctaataac cccttatcaa tttaagaaat aatccgcata agcccccgct
7861 taaaaattgg tatcagagcc atgaataggt ctatgaccaa aactcaagag gataaaacct
7921 caccaaaata cgaaagagtt cttaactcta aagataaaag atctttcaag atcaaaacta
7981 gttccctcac accggtgacg gggatcgcat gcgatatctc gagatctagc ttggcgtaat
8041 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
8101 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
8161 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
8221 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
8281 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
8341 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
8401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
8461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
8521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
8581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
8641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
8701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
8761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
8821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
8881 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
8941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
9001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
9061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
9121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
9181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
9241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
9301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
9361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
9421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
9481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
9541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
9601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
9661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
9721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
9781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
9841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
9901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
9961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
10021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
10081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
10141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcca
10201 gt
//
