| 质粒名称: | pBMTBX-3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26074 |
| 存储实验室: | Ryan Gill |
|
载体骨架基本信息 |
|
| 载体名称: | pBMTBX-3 |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 4922 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI（销毁时未知） |
| 3' 克隆位点: | HincII（销毁时未知） |
| 5' 测序引物 | atacccgttt ttttgggcta |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBMTBX-3 was a gift from Ryan Gill (Addgene plasmid # 26074 ; http://n2t.net/addgene:26074 ; RRID:Addgene_26074) |
| 参考文献部分: | Broad-host-range vectors for protein expression across gram negative hosts. Prior JE, Lynch MD, Gill RT. Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32. 10.1002/bit.22695 PubMed 20148414 |
买家导航
pBMTBX3载体基本信息
pBMTBX3载体图谱质粒图谱和多克隆位点信息
pBMTBX3载体简介
pBMTBX3载体序列
LOCUS pBMTBX-3 4922 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBMTBX-3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4922)
AUTHORS Prior JE, Lynch MD, Gill RT
TITLE Broad-host-range vectors for protein expression across gram negative
hosts.
JOURNAL Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32.
PUBMED 20148414
REFERENCE 2 (bases 1 to 4922)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4922
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 60..96
/label=soxR terminator
/note="bidirectional E. coli soxR transcription terminator"
rep_origin 1347..2116
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
CDS 2117..2779
/codon_start=1
/product="replication protein for the broad-host-range
plasmid pBBR1 from Bordetella bronchiseptica"
/label=pBBR1 Rep
/translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
CDS 2876..3535
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(2942..2961)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
terminator 3554..3585
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
CDS complement(3613..4491)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 4518..4802
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
primer_bind 4725..4744
/label=pBAD Forward
/note="For vectors with E. coli araBAD promoter, forward
primer"
ORIGIN
1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga
2881 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
2941 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
3001 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
3061 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
3121 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
3181 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
3241 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
3301 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
3361 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
3421 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
3481 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg
3541 tggatccccc tcaagtcaaa agcctccggt cggaggcttt tgactttctg ctatggaggt
3601 caggtatgat ttttatgaca acttgacggc tacatcattc actttttctt cacaaccggc
3661 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
3721 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
3781 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
3841 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
3901 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
3961 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
4021 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
4081 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc
4141 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
4201 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
4261 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt
4321 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
4381 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
4441 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
4501 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg
4561 cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct
4621 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg
4681 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt
4741 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
4801 atacccgttt ttttgggcta gcaccgccta tctcgtgtga gataggcgga gatacgaact
4861 ttaagaagga gatatacaca tgttaaaatg gtcagtattg agcgatatct agagaattcg
4921 tc
//
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