| 质粒名称: | pST-KT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 44561 |
| 存储实验室: | Vinay Nandicoori |
|
载体骨架基本信息 |
|
| 载体名称: | pVV16, pSE100, pMC1m |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达；大肠杆菌-分枝杆菌穿梭载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | UV15 promoter |
| 标签/融合蛋白: |
His tag（主干上的N终端）
Flag tag（主干上的C终端）
|
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pST-KT was a gift from Vinay Nandicoori (Addgene plasmid # 44561 ; http://n2t.net/addgene:44561 ; RRID:Addgene_44561) |
| 参考文献部分: | Development of a new generation of vectors for gene expression, gene replacement, and protein-protein interaction studies in mycobacteria. Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, Nandicoori VK. Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi: 10.1128/AEM.03695-12. Epub 2013 Jan 11. 10.1128/AEM.03695-12 PubMed 23315736 |
买家导航
pSTKT载体基本信息
pSTKT载体图谱质粒图谱和多克隆位点信息
pSTKT载体简介
pSTKT载体序列
LOCUS pST-KT 5348 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION for ectopic inducible expression of mycobacterial proteins.
ACCESSION .
VERSION .
KEYWORDS pST-KT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5348)
AUTHORS Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U,
Nandicoori VK
TITLE Development of a new generation of vectors for gene expression, gene
replacement, and protein-protein interaction studies in
mycobacteria.
JOURNAL Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi:
10.1128/AEM.03695-12. Epub 2013 Jan 11.
PUBMED 23315736
REFERENCE 2 (bases 1 to 5348)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5348
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 179..196
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 335..352
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 425..448
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
terminator 467..509
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 538..584
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind complement(734..753)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 745..1560
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(818..837)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
rep_origin 1892..2480
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2381..2400
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 2789..3412
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
primer_bind complement(2862..2881)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
ORIGIN
1 agtactaata ttggatcgtc ggcaccgtca cggccgtggg aggcggcacg atccgcgacg
61 tgatgatcgg ccgcatcccc acggtgctgc gcagtgagct ctacgccatc ccggcgttga
121 tctgtgcgtt cgcacgcaca ggcccggtgt gagaagggtc tctgacgagc gggagaactc
181 cctatcagtg atagagtttg tcctccctat cagtgataga taggctctgg gagtacccgt
241 gtgtacgacc agcacggcat acatcatttc gacgccgaga gattcgccgc ccgaaatgag
301 cacgatccgc atgcggagga atcacttcgc aatgcaccac caccaccacc atatgggatc
361 cgatatctct agagcggccg cacagctggg gcccgaattc ctgcagcacg tgttaattaa
421 gcttgactac aaggacgacg acgacaagta aaagctatca gttaactaaa acgaaaggct
481 cagtcgaaag actgggcctt tcgttttata actagcgtac gatcgactgc caggcatcaa
541 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatgccatc atggccgcgg
601 tgatcagcta gccacctgac gtcggggggg ggggaaagcc acgttgtgtc tcaaaatctc
661 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
721 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg
781 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
841 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agccccatgc gccagagttg
901 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
961 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
1021 gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa
1081 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
1141 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
1201 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
1261 tggcctgttg aacaagtctg gaaagaaatg cataatcttt tgccattctc accggattca
1321 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
1381 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
1441 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
1501 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
1561 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
1621 ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg
1681 acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca
1741 acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg
1801 cctggtatga gtcagcaaca ccttcttcac gaggcagacc tcactagttc cactgagcgt
1861 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
1921 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
1981 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
2041 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
2101 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
2161 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
2221 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
2281 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
2341 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
2401 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
2461 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
2521 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
2581 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcaacgcgt
2641 tacgtaaata ttggatcgtc gcaccgggtt aagcctggag tgcggtggtg cctggtcggc
2701 attttcgcag tcgagggctc tcgtgtagcc tgggcgagtt gccgacgcag gcgaccctcc
2761 tgccacggat cggaggaatc acttcgcaat gtcaagatta gataaaagta aagtgattaa
2821 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc
2881 ccagaaactt ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgagccct
2941 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga
3001 aggggaaagc tggcaagatt ttttgcgtaa taacgctaaa agttttagat gtgctttact
3061 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga
3121 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaacgcgtt
3181 atatgcactc agcgctgtgg ggcattttac cttaggttgc gtattggaag atcaagagca
3241 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg
3301 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga
3361 attgatcatc tgcggattag aaaaacaact taaatgtgaa agtgggtctt aatgaccatg
3421 ggctacgtag agcccaccag ctccgtaagt tcgggtgctg tgtggctcgt acccgcgcat
3481 tcaggcggca gggggtctaa cgggtctaag gcggcgtgta cggccgccac agcggctctt
3541 agcggcccgg aaacgtcctc gaaacgacgc atgtgttcct cctggttggt acaggtggtt
3601 gggggtgctc ggctgtcgct ggtgtttcat catcagggct cgacgggaga gcgggggagt
3661 gtgcagttgt ggggtggccc ctcagcgaaa tatctgactt ggagctcgtg tcggaccata
3721 caccggtgat taatcgtggt ttattatcaa gcgtgagcca cgtcgccgac gaatttgagc
3781 agctctggct gccgtactgg tccctggcaa gcgacgatct gctcgagggg atctaccgcc
3841 aaagccgcgc gtcggcccta ggccgccggt acatcgaggc gaacccaaca gcgctggcaa
3901 acctgctggt cgtggacgta gaccatccag acgcagcgct ccgagcgctc agcgcccggg
3961 ggtcccatcc gctgcccaac gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag
4021 tgtgggcact caacgcccct gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat
4081 acatggcggc gtgcgccgaa ggccttcggc gcgccgtcga tggcgaccgc agttactcag
4141 gcctcatgac caaaaacccc ggccacatcg cctgggaaac ggaatggctc cactcagatc
4201 tctacacact cagccacatc gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc
4261 gtcagcagac cacgtacaaa gcggctccga cgccgctagg gcggaattgc gcactgttcg
4321 attccgtcag gttgtgggcc tatcttcccg ccctcatgcg gatctacctg ccgacccgga
4381 acgtggacgg actcggccgc gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc
4441 cgtgcaacga cgtgtgtccc ggaccgctac cggacagcga ggtccgcgcc atcgccaaca
4501 gcatttggcg ttggatcaca accaagtcgc gcatttgggc ggacgggatc gtggtctacg
4561 aggccacact cagtgcgcgc catgcggcca tctcgcggaa gggcgcagca gcgcgcacgg
4621 cggcgagcac agttgcgcgg cgcgcaaagt ccgcgtcagc catggaggca ttgctatgag
4681 cgacggctac agcgacggct acagcgacgg ctacaactgg cagccgactg tccgcaaaaa
4741 gcggcgcgtg accgccgccg aaggcgctcg aatcaccgga ctatccgaac gccacgtcgt
4801 ccggctcgtg gcgcaggaac gcagcgagtg gttcgccgag caggctgcac gccgcgaacg
4861 catccgcgcc tatcacgacg acgagggcca ctcttggccg caaacggcca aacatttcgg
4921 gctgcatctg gacaccgtta agcgactcgg ctatcgggcg aggaaagagc gtgcggcaga
4981 acaggaagcg gctcaaaagg cccacaacga agccgacaat ccaccgctgt tctaacgcaa
5041 ttggggagcg ggtgtcgcgg gggttccgtg gggggttccg ttgcaacggg tcggacaggt
5101 aaaagtcctg gtagacgcta gttttctggt ttgggccatg cctgtctcgt tgcgtgtttc
5161 gttgcgtccg ttttgaatac cagccagacg agacggggtt ctacgaatct tggtcgatac
5221 caagccattt ccgctgaata tcgtggagct caccgccaga atcggtggtt gtggtgatgt
5281 acgtggcgaa ctccgttgta gtgcttgtgg tggcatccgt ggcggtaccg cctgacgccc
5341 cccggatc
//
