| 质粒名称: | psPAX2-D64V |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 63586 |
| 存储实验室: | David Rawlings, Andrew Scharenberg |
|
载体骨架基本信息 |
|
| 载体名称: | psPAX2 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 10703 bp |
| 载体修饰: | -- |
| 载体类型: | 整合酶缺陷慢病毒包装载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Packaging plasmid for integrase deficient lentiviral vectors |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | |
| 参考文献部分: | Tracking genome engineering outcome at individual DNA breakpoints. Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, Scharenberg AM. Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648. 10.1038/nmeth.1648 PubMed 21743461 |
买家导航
psPAX2D64V载体基本信息
psPAX2D64V载体图谱质粒图谱和多克隆位点信息
psPAX2D64V载体简介
psPAX2-D64V来源于psPAX2质粒。它是一种包装质粒，因为它的整合酶基因中存在一个点突变，所以可以用于制造整合酶缺陷慢病毒载体。
psPAX2D64V载体序列
LOCUS psPAX2-D64V 10644 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging
plasmid for making Integrase deficient lentiviral vectors since has
a point mutation in the integrase gene..
ACCESSION .
VERSION .
KEYWORDS psPAX2-D64V
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10644)
AUTHORS Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ,
Scharenberg AM
TITLE Tracking genome engineering outcome at individual DNA breakpoints.
JOURNAL Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
PUBMED 21743461
REFERENCE 2 (bases 1 to 10644)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10644
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..196
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 47..182
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 109..128
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 202..336
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(239..258)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 293..312
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(404..421)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(575..1163)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(655..674)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1334..2194)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1957..1976
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2195..2299)
/gene="bla"
/label=AmpR promoter
enhancer 2330..2709
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
intron 2922..3938
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 3862..3884
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 3946..3965
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 4044..5546
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/label=HIV-1 gag
/translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
CDS 5339..8350
/codon_start=1
/gene="pol"
/product="pol protein from human immunodeficiency virus 1"
/label=HIV-1 pol
/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
MAGDDCVASRQDED"
misc_feature 8035..8152
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
misc_feature 8924..9157
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 9342..9386
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 9680..9706
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
primer_bind complement(9991..10010)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 10056..10111
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(10110..10129)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(10472..10488)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10472..10488)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10485..10507)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10496..10512
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10520..10550)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10565..10586
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
61 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
121 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
181 cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc
241 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
301 tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac
361 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
421 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
481 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
541 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
601 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
661 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
721 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
781 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
841 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
901 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
961 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
1021 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
1081 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
1141 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
1201 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
1261 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
1321 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
1381 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
1441 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
1501 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
1561 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
1621 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
1681 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
1741 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
1801 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
1861 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
1921 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
1981 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
2041 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
2101 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
2161 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
2221 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
2281 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
2341 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
2401 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
2461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
2521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
2581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
2641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
2701 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
2761 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagggcg aggcggagag
2821 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
2881 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
2941 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
3001 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
3061 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
3121 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
3181 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
3241 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
3301 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
3361 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
3421 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
3481 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
3541 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
3601 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
3661 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
3721 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
3781 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
3841 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
3901 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
3961 ttgtgctgtc tcatcatttt ggcaaagaat tcgggccgcg ttgacgcgca cggcaagagg
4021 cgaggggcgg cgactggtga gagatgggtg cgagagcgtc agtattaagc gggggagaat
4081 tagatcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
4141 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
4201 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
4261 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
4321 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
4381 aaaaagcaca gcaagcagca gctgacacag gacacagcaa tcaggtcagc caaaattacc
4441 ctatagtgca gaacatccag gggcaaatgg tacatcaggc catatcacct agaactttaa
4501 atgcatgggt aaaagtagta gaagagaagg ctttcagccc agaagtgata cccatgtttt
4561 cagcattatc agaaggagcc accccacaag atttaaacac catgctaaac acagtggggg
4621 gacatcaagc agccatgcaa atgttaaaag agaccatcaa tgaggaagct gcagaatggg
4681 atagagtgca tccagtgcat gcagggccta ttgcaccagg ccagatgaga gaaccaaggg
4741 gaagtgacat agcaggaact actagtaccc ttcaggaaca aataggatgg atgacacata
4801 atccacctat cccagtagga gaaatctata aaagatggat aatcctggga ttaaataaaa
4861 tagtaagaat gtatagccct accagcattc tggacataag acaaggacca aaggaaccct
4921 ttagagacta tgtagaccga ttctataaaa ctctaagagc cgagcaagct tcacaagagg
4981 taaaaaattg gatgacagaa accttgttgg tccaaaatgc gaacccagat tgtaagacta
5041 ttttaaaagc attgggacca ggagcgacac tagaagaaat gatgacagca tgtcagggag
5101 tggggggacc cggccataaa gcaagagttt tggctgaagc aatgagccaa gtaacaaatc
5161 cagctaccat aatgatacag aaaggcaatt ttaggaacca aagaaagact gttaagtgtt
5221 tcaattgtgg caaagaaggg cacatagcca aaaattgcag ggcccctagg aaaaagggct
5281 gttggaaatg tggaaaggaa ggacaccaaa tgaaagattg tactgagaga caggctaatt
5341 ttttagggaa gatctggcct tcccacaagg gaaggccagg gaattttctt cagagcagac
5401 cagagccaac agccccacca gaagagagct tcaggtttgg ggaagagaca acaactccct
5461 ctcagaagca ggagccgata gacaaggaac tgtatccttt agcttccctc agatcactct
5521 ttggcagcga cccctcgtca caataaagat aggggggcaa ttaaaggaag ctctattaga
5581 tacaggagca gatgatacag tattagaaga aatgaatttg ccaggaagat ggaaaccaaa
5641 aatgataggg ggaattggag gttttatcaa agtaagacag tatgatcaga tactcataga
5701 aatctgcgga cataaagcta taggtacagt attagtagga cctacacctg tcaacataat
5761 tggaagaaat ctgttgactc agattggctg cactttaaat tttcccatta gtcctattga
5821 gactgtacca gtaaaattaa agccaggaat ggatggccca aaagttaaac aatggccatt
5881 gacagaagaa aaaataaaag cattagtaga aatttgtaca gaaatggaaa aggaaggaaa
5941 aatttcaaaa attgggcctg aaaatccata caatactcca gtatttgcca taaagaaaaa
6001 agacagtact aaatggagaa aattagtaga tttcagagaa cttaataaga gaactcaaga
6061 tttctgggaa gttcaattag gaataccaca tcctgcaggg ttaaaacaga aaaaatcagt
6121 aacagtactg gatgtgggcg atgcatattt ttcagttccc ttagataaag acttcaggaa
6181 gtatactgca tttaccatac ctagtataaa caatgagaca ccagggatta gatatcagta
6241 caatgtgctt ccacagggat ggaaaggatc accagcaata ttccagtgta gcatgacaaa
6301 aatcttagag ccttttagaa aacaaaatcc agacatagtc atctatcaat acatggatga
6361 tttgtatgta ggatctgact tagaaatagg gcagcataga acaaaaatag aggaactgag
6421 acaacatctg ttgaggtggg gatttaccac accagacaaa aaacatcaga aagaacctcc
6481 attcctttgg atgggttatg aactccatcc tgataaatgg acagtacagc ctatagtgct
6541 gccagaaaag gacagctgga ctgtcaatga catacagaaa ttagtgggaa aattgaattg
6601 ggcaagtcag atttatgcag ggattaaagt aaggcaatta tgtaaacttc ttaggggaac
6661 caaagcacta acagaagtag taccactaac agaagaagca gagctagaac tggcagaaaa
6721 cagggagatt ctaaaagaac cggtacatgg agtgtattat gacccatcaa aagacttaat
6781 agcagaaata cagaagcagg ggcaaggcca atggacatat caaatttatc aagagccatt
6841 taaaaatctg aaaacaggaa agtatgcaag aatgaagggt gcccacacta atgatgtgaa
6901 acaattaaca gaggcagtac aaaaaatagc cacagaaagc atagtaatat ggggaaagac
6961 tcctaaattt aaattaccca tacaaaagga aacatgggaa gcatggtgga cagagtattg
7021 gcaagccacc tggattcctg agtgggagtt tgtcaatacc cctcccttag tgaagttatg
7081 gtaccagtta gagaaagaac ccataatagg agcagaaact ttctatgtag atggggcagc
7141 caatagggaa actaaattag gaaaagcagg atatgtaact gacagaggaa gacaaaaagt
7201 tgtcccccta acggacacaa caaatcagaa gactgagtta caagcaattc atctagcttt
7261 gcaggattcg ggattagaag taaacatagt gacagactca caatatgcat tgggaatcat
7321 tcaagcacaa ccagataaga gtgaatcaga gttagtcagt caaataatag agcagttaat
7381 aaaaaaggaa aaagtctacc tggcatgggt accagcacac aaaggaattg gaggaaatga
7441 acaagtagat aaattggtca gtgctggaat caggaaagta ctatttttag atggaataga
7501 taaggcccaa gaagaacatg agaaatatca cagtaattgg agagcaatgg ctagtgattt
7561 taacctacca cctgtagtag caaaagaaat agtagccagc tgtgataaat gtcagctaaa
7621 aggggaagcc atgcatggac aagtagactg tagcccagga atatggcagc tagtttgtac
7681 acatttagaa ggaaaagtta tcttggtagc agttcatgta gccagtggat atatagaagc
7741 agaagtaatt ccagcagaga cagggcaaga aacagcatac ttcctcttaa aattagcagg
7801 aagatggcca gtaaaaacag tacatacaga caatggcagc aatttcacca gtactacagt
7861 taaggccgcc tgttggtggg cggggatcaa gcaggaattt ggcattccct acaatcccca
7921 aagtcaagga gtaatagaat ctatgaataa agaattaaag aaaattatag gacaggtaag
7981 agatcaggct gaacatctta agacagcagt acaaatggca gtattcatcc acaattttaa
8041 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
8101 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
8161 ttacagggac agcagagatc cagtttggaa aggaccagca aagctcctct ggaaaggtga
8221 aggggcagta gtaatacaag ataatagtga cataaaagta gtgccaagaa gaaaagcaaa
8281 gatcatcagg gattatggaa aacagatggc aggtgatgat tgtgtggcaa gtagacagga
8341 tgaggattaa cacatggaat tctgcaacaa ctgctgttta tccatttcag aattgggtgt
8401 cgacatagca gaataggcgt tactcgacag aggagagcaa gaaatggagc cagtagatcc
8461 tagactagag ccctggaagc atccaggaag tcagcctaaa actgcttgta ccaattgcta
8521 ttgtaaaaag tgttgctttc attgccaagt ttgtttcatg acaaaagcct taggcatctc
8581 ctatggcagg aagaagcgga gacagcgacg aagagctcat cagaacagtc agactcatca
8641 agcttctcta tcaaagcagt aagtagtaca tgtaatgcaa cctataatag tagcaatagt
8701 agcattagta gtagcaataa taatagcaat agttgtgtgg tccatagtaa tcatagaata
8761 taggaaaatg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
8821 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
8881 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
8941 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
9001 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
9061 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
9121 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
9181 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
9241 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
9301 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
9361 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
9421 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
9481 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
9541 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
9601 acagagacag atccattcga ttagtgaacg gatccttggc acttatctgg gacgatctgc
9661 ggagcctgtg cctcttcagc taccaccgct tgagagactt actcttgatt gtaacgagga
9721 ttgtggaact tctgggacgc agggggtggg aagccctcaa atattggtgg aatctcctac
9781 aatattggag tcaggagcta aagaatagtg ctgttagctt gctcaatgcc acagccatag
9841 cagtagctga ggggacagat agggttatag aagtagtaca aggagcttgt agagctattc
9901 gccacatacc tagaagaata agacagggct tggaaaggat tttgctataa gctcgaaaca
9961 accggtacct ctagaactat agctagcaga tctttttccc tctgccaaaa attatgggga
10021 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
10081 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
10141 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
10201 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
10261 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
10321 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
10381 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
10441 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
10501 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
10561 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
10621 gaaacctgtc gtgccagcgg atcc
//
