| 质粒名称: | pLemiR-NS |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 32809 |
| 存储实验室: | Jerry Crabtree |
|
载体骨架基本信息 |
|
| 载体名称: | pLemiR |
| 载体出品公司: | Open Biosystems |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 11315 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | non-specific hairpin |
| 物种来源: | -- |
| 插入大小: | 350 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 非连接克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | GATAACTCGACTGTTTGAATGAGGC |
| 3' 测序引物 | AGGGAGAGGGGCGGAATTTG |
|
文献引用方法 |
|
| 材料和方法部分: | pLemiR-NS was a gift from Jerry Crabtree (Addgene plasmid # 32809 ; http://n2t.net/addgene:32809 ; RRID:Addgene_32809) |
| 参考文献部分: | MicroRNA-mediated conversion of human fibroblasts to neurons. Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer C, Dolmetsch RE, Tsien RW, Crabtree GR. Nature. 2011 Jul 13. doi: 10.1038/nature10323. 10.1038/nature10323 PubMed 21753754 |
买家导航
pLemiRNS载体基本信息
pLemiRNS载体图谱质粒图谱和多克隆位点信息
pLemiRNS载体简介
pLemiRNS载体序列
LOCUS pLemiR-NS 11662 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLemiR-NS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11662)
AUTHORS Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer
C, Dolmetsch RE, Tsien RW, Crabtree GR
TITLE MicroRNA-mediated conversion of human fibroblasts to neurons.
JOURNAL Nature. 2011 Jul 13. doi: 10.1038/nature10323.
PUBMED 21753754
REFERENCE 2 (bases 1 to 11662)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11662
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2063..2180
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(2245..2619)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
regulatory 2616..2625
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(2629..2647)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(2639..2686)
/label=EM7 promoter
/note="synthetic bacterial promoter "
enhancer 2753..3056
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3057..3260
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3211..3231
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 3257..3281
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 3364..4059
/codon_start=1
/product="red fluorescent protein from Entacmaea
quadricolor"
/label=TurboRFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
DLPSKLGHR"
ncRNA 4069..4190
/label=5' miR-30a
/note="sequence upstream of the 71-nt precursor of the
human miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
ncRNA 4218..4232
/label=miR-30a loop
/note="loop from the the 71-nt precursor of the human
miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
ncRNA 4260..4386
/label=3' miR-30a
/note="sequence downstream of the 71-nt precursor of the
human miR-30a microRNA (Zeng et al., 2002)"
/ncRNA_class="miRNA"
misc_feature 4440..5012
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4607..4624)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4834..4853
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5015..5617
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind 5514..5534
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 5633..6221
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5686..5706)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6104..6115)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6428..6661
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(6762..6779)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6768..6992
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7038..7466
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7125..7144)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7335..7356
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7475..7495)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7480..7809
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7660..7795
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7722..7741
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 9017..9138
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9187..9203)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9187..9203)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9200..9222)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9211..9227
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9235..9265)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9280..9301
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9418..9435)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9589..10177)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9669..9688)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10348..11208)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10971..10990
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11209..11313)
/gene="bla"
/label=AmpR promoter
polyA_signal 11361..11495
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
2701 cgaggttcta gagaccggtg gttaacgcgg atcccatcga ttgggagttc cgcgttacat
2761 aacttaccgt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
2821 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
2881 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
2941 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
3001 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
3061 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
3121 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
3181 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
3241 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
3301 gctgttttga cctccataga agacaccgac tctagctaga ggctagcgct accggtcgcc
3361 accatgagcg agctgatcaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
3421 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
3481 accatgaaga tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
3541 accagcttca tgtacggcag caaagccttc atcaaccaca cccagggcat ccccgacttc
3601 tttaagcagt ccttccctga gggcttcaca tgggagagaa tcaccacata cgaagacggg
3661 ggcgtgctga ccgctaccca ggacaccagc ttccagaacg gctgcatcat ctacaacgtc
3721 aagatcaacg gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacacgcggc
3781 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctgagagg ccacagccag
3841 atggccctga agctcgtggg cgggggctac ctgcactgct ccttcaagac cacatacaga
3901 tccaagaaac ccgctaagaa cctcaagatg cccggcttcc acttcgtgga ccacagactg
3961 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgagat ggctgtggcc
4021 aagtactgcg acctccctag caaactgggg cacagataac tcgactgttt gaatgaggct
4081 tcagtacttt acagaatcgt tgcctgcaca tcttggaaac acttgctggg attacttctt
4141 caggttaacc caacagaagg ctcgagaagg tatattgctg ttgacagtga gcgatctcgc
4201 ttgggcgaga gtaagtagtg aagccacaga tgtacttact ctcgcccaag cgagagtgcc
4261 tactgcctcg gaattcaagg ggctacttta ggagcaatta tcttgtttac taaaactgaa
4321 taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta taaattaaat
4381 cacttttttc aattggaaga ctaatgcggc cggccacgcg tcggcggccg caaattccgc
4441 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
4501 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
4561 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
4621 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
4681 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
4741 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
4801 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
4861 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
4921 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
4981 gacgtggttt tcctttgaaa aacacgataa taccatggcc accgagtaca agcccacggt
5041 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
5101 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
5161 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
5221 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
5281 cgccgagatc ggctcgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
5341 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
5401 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
5461 ggaggcggcc gagcgcgctg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
5521 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
5581 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtt cgcgtctgga acaatcaacc
5641 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
5701 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
5761 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
5821 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
5881 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
5941 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
6001 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
6061 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
6121 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
6181 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
6241 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
6301 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
6361 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
6421 aggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
6481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
6541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
6601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
6661 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
6721 gagagtgaga ggccttgaca ttgtttaaac ccgctgatca gcctcgactg tgccttctag
6781 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
6841 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
6901 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
6961 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
7021 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
7081 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
7141 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
7201 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
7261 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
7321 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
7381 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
7441 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
7501 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
7561 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
7621 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
7681 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
7741 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
7801 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgatg
7861 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
7921 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
7981 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
8041 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
8101 gaattaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
8161 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
8221 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
8281 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
8341 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
8401 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
8461 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
8521 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
8581 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
8641 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
8701 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
8761 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
8821 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
8881 ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
8941 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
9001 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
9061 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
9121 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
9181 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
9241 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
9301 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
9361 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
9421 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
9481 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
9541 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
9601 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
9661 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
9721 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
9781 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
9841 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
9901 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
9961 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
10021 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
10081 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
10141 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
10201 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
10261 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
10321 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
10381 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
10441 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
10501 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
10561 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
10621 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
10681 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
10741 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
10801 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
10861 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
10921 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
10981 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
11041 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
11101 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
11161 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
11221 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
11281 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
11341 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
11401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
11461 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
11521 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
11581 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
11641 tatgtactac aaacttagta gt
//
