| 质粒名称: | Plasmid L5 attB::Pleft* mCherry |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 169411 |
| 存储实验室: | Clifton Barry III |
|
载体骨架基本信息 |
|
| 载体名称: | pMV306 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 4064 bp |
| 载体修饰: | -- |
| 载体类型: | Unspecified |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | pLeft 、mCherry |
| 物种来源: | -- |
| 插入大小: | 400 bp、 714 bp |
| 基因突变: | |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
|
文献引用方法 |
|
| 材料和方法部分: | Plasmid L5 attB::Pleft* mCherry was a gift from Clifton Barry III (Addgene plasmid # 169411 ; http://n2t.net/addgene:169411 ; RRID:Addgene_169411) |
| 参考文献部分: | Development and Optimization of Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs. Kolbe K, Bell AC, Prosser GA, Assmann M, Yang HJ, Forbes HE, Gallucci S, Mayer-Barber KD, Boshoff HI, Barry Iii CE. Front Microbiol. 2020 Dec 9;11:591866. doi: 10.3389/fmicb.2020.591866. eCollection 2020. 10.3389/fmicb.2020.591866 PubMed 33362741 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Plasmid L5 attB::Pleft* mCherry 4064 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis
Reporter Constructs for mCherry.
ACCESSION .
VERSION .
KEYWORDS Plasmid L5 attB::Pleft* mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4064)
AUTHORS Kolbe K, Bell AC, Prosser GA, Assmann M, Yang HJ, Forbes HE,
Gallucci S, Mayer-Barber KD, Boshoff HI, Barry Iii CE
TITLE Development and Optimization of Chromosomally-Integrated Fluorescent
Mycobacterium tuberculosis Reporter Constructs.
JOURNAL Front Microbiol. 2020 Dec 9;11:591866. doi:
10.3389/fmicb.2020.591866. eCollection 2020.
PUBMED 33362741
REFERENCE 2 (bases 1 to 4064)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4064
/organism="synthetic DNA construct"
/mol_type="other DNA"
RBS 245..253
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 269..286
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 1065..1111
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind complement(1920..1939)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 1931..2746
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(2004..2023)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
rep_origin 3077..3665
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3566..3585
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3895..3914
/label=T7
/note="T7 promoter, forward primer"
promoter 3895..3913
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 4030..4047
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 aatacgcaaa ccgcctctcc cagatctgat atcgctagag gaaacagcta tgaccatgat
61 tacgccaagc ttgcatgcct gcagctaggg caccaatttg cgattagggc ttgacagcca
121 cccggccagt agtgcattct tgtgtcaccg cagcagcaag gcggtaggcg gatccgagag
181 gatcgtgccg gtgccggtga agagagattc gccgcccgaa atgagcacga tccgcatgct
241 taattaagga ggtatctcca tgagcggtca ccatcaccat caccacggcg gtagcgccat
301 gtctgtctcg aagggcgagg aggacaacat ggcgatcatc aaggagttca tgcgcttcaa
361 ggtccacatg gagggctcgg tcaacggcca cgagttcgag atcgagggcg agggcgaggg
421 ccgcccgtac gagggcaccc agaccgccaa gctgaaggtc accaagggcg gcccgctgcc
481 gttcgcctgg gacatcctgt cgccgcagtt catgtacggc agcaaggcct acgtcaagca
541 cccggccgac atcccggact acctgaagct gtcgttcccg gagggcttca agtgggagcg
601 cgtcatgaac ttcgaggacg gcggcgtcgt caccgtcacc caggactcgt cgctgcagga
661 cggcgagttc atctacaagg tcaagctgcg gggcaccaac ttcccgtcgg acggcccggt
721 catgcagaag aagaccatgg gctgggaggc ctcgtcggag cgcatgtacc cggaggacgg
781 cgccctgaag ggcgagatca agcagcggct gaagctgaag gacggcggcc actacgacgc
841 cgaggtcaag accacctaca aggccaagaa gccggtccag ctgccgggcg cctacaacgt
901 gaacatcaag ctggacatca ccagccacaa cgaggactac accatcgtcg agcagtacga
961 gcgcgccgag ggccgccaca gcaccggcgg catggacgag ctgtacaagt gaaagcttat
1021 cgatgtcgac gtagttaact agcgtacgat cgactgccag gcatcaaata aaacgaaagg
1081 ctcagtcgaa agactgggcc tttcgtttta tgccatcatg gccgcggtga tcagctagag
1141 ccgtgaacga cagggcgaac gccagcccgc cgacggcgag ggttccgacc gctgcaactc
1201 ccggtgcaac cttgtcccgg tctattctct tcactgcacc agctccaatc tggtgtgaat
1261 gcccctcgtc tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc gaaagtagcc
1321 agatcaggga tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac aagagttgca
1381 gacccctgga aagaaaaatg gccagagggc gaaaacaccc tctgaccagc ggagcgggcg
1441 acgggaatcg aacccgcgta gctagtttgg aagaatgggt gtctgccgac cacatatatg
1501 ggccggtcaa gataggtttt taccccctct cggctgcatc ctctaagtgg aaagaaattg
1561 caggtcgtag aagcgcgttg aagcctgaga gttgcacagg agttgcaacc cggtagcctt
1621 gttcacgacg agaggagacc tagggatcct cccaaggaca ctgagtccta aagagggggg
1681 tttcttgtca gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg
1741 cggagacctg ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagctagcc
1801 acctgacgtc gggggggggg aaagccacgt tgtgtctcaa aatctctgat gttacattgc
1861 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
1921 aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc
1981 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
2041 tgcgacaatc tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg
2101 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
2161 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
2221 caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
2281 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
2341 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
2401 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
2461 agtctggaaa gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg
2521 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
2581 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
2641 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
2701 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
2761 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata
2821 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt
2881 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcacc
2941 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca
3001 gcaacacctt cttcacgagg cagacctcac tagttccatg agcgtcagac cccgtagaaa
3061 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
3121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
3181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
3241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
3301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
3361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
3421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
3481 ccacgcttcc cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
3541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
3601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
3661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
3721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
3781 gagctgatac cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtacca
3841 gatctttaaa tctagacgat gataagcggt caaacatgag aattcgcggc cgcataatac
3901 gactcactat agggatctta attaaggcgc ctcatgttct ttcctgcgtt atcccctgat
3961 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
4021 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gccc
//
