pYC56

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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pYC56载体质粒基本信息

出品人: Irene Castano Navarro(2015)
载体名称: pYC56
质粒类型: 酿酒酵母表达载体;整合型载体;FLP/FRT系统载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 6633 bp
5' 测序引物及序列: M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’
3' 测序引物及序列: --
载体标签: 红色荧光蛋白mCherry(C ter)
载体抗性: 氨苄青霉素
筛选标记: URA3,Nourseothricin
克隆菌株: DH10B感受态细胞 ,30°C
宿主细胞(系): 出芽酵母(酿酒酵母)、白色念珠菌
备注: --
产品目录号: #63910
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pYC56质粒图谱载体图谱和pYC56载体序列质粒序列多克隆位点信息

pYC56载体图谱



pYC56多克隆位点

pYC56质粒载体简介

酿酒酵母整合型载体还有: 

pYC44
pYC56   红色荧光标签
pYC54   黄色荧光标签
pYC52   青色荧光标签
pYC50   13XMyc标签
pYC48   HA标签
pYC46   3XFlag标签



pYC56质粒序列载体序列

LOCUS       pYC56	6633 bp 	DNA	SYN
DEFINITION  pYC56
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="pYC56"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     promoter        196..409
                     /label="URA3_promoter"
     gene            411..1211
                     /label="URA3"
                     /gene="URA3"
     CDS             411..1214
                     /label="ORF frame 3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             complement(446..922)
                     /label="ORF frame 3"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    complement(1814..1962)
                     /label="lacZ_a"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    1928..1950
                     /label="M13_pUC_fwd_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1943..1959
                     /label="M13_forward20_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1966..1984
                     /label="T7_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    2017..2033
                     /label="pBluescriptSK_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     CDS             2037..2747
                     /label="ORF frame 3"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     gene            2040..2744
                     /label="mCherry"
                     /gene="mCherry"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     misc_feature    2217..2270
                     /label="Q66M (mCherry)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
     CDS             complement(3439..3942)
                     /label="ORF frame 1"
                     /translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF
                     NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW
                     INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRID
                     PVVYVRM*"
     CDS             3478..4050
                     /label="ORF frame 1"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(4372..4391)
                     /label="T3_promoter"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(4408..4426)
                     /label="M13_reverse_primer"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     misc_feature    complement(4425..4447)
                     /label="M13_pUC_rev_primer"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     rep_origin      complement(4799..5418)
                     /label="pBR322_origin"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     gene            complement(5573..6433)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     CDS             complement(5573..6433)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(6475..6503)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
  181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
  241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
  301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
  361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG
  421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
  481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
  541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
  601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
  661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
  721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
  781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
  841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
  901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
  961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
 1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
 1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
 1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
 1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
 1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
 1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
 1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
 1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA
 1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
 1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
 1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
 1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
 1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg
 1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
 1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
 1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta
 1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgG ATCCTAATGG
 2041 TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC
 2101 ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC
 2161 CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG
 2221 CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG
 2281 CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA
 2341 TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG
 2401 AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC
 2461 AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC
 2521 TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCTGAGG
 2581 TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA
 2641 TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG
 2701 CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGTAGaga tccgaagttc
 2761 ctatactttc tagagaatag gaacttcgtg cgcttttgaa ccacgtaaag tgctgtaaaa
 2821 cagctctatt caatgaggta aatgataaag tttataagag accaaaaaat tcaaaaaaaa
 2881 aattgaaaaa ttatattggt atttatatga aacaatagtt cagttcaaag cgctagcttt
 2941 tctcatctca actaaccggt attaggtggg tatttattta atatgggaac aagaaatgtg
 3001 acactaatgt tcatgttttt attttcatta tttattctat atggtttggg acttgatgtt
 3061 atgtcttaat tcatagaGAT CCgcttgcct cgtccccgcc gggtcacccg gccagcgaca
 3121 tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg
 3181 actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt
 3241 ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc tgcgagcagg
 3301 gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata
 3361 taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc
 3421 taggatacag ttctcacatc acatccgaac ataaacaacc gGATCgatcc tacaattATG
 3481 TCTACTACTT TGGATGATAC TGCTTATAGA TACAGAACTT CTGTTCCAGG TGATGCTGAA
 3541 GCTATTGAAG CTTTGGATGG TTCTTTCACT ACCGATACTG TTTTTAGAGT TACTGCTACT
 3601 GGTGATGGTT TCACTTTGAG AGAAGTTCCA GTTGATCCAC CATTGACTAA GGTTTTCCCA
 3661 GATGATGAAT CCGATGATGA ATCCGATGCT GGTGAAGATG GTGATCCAGA TTCTAGAACT
 3721 TTCGTTGCTT ATGGTGATGA TGGTGATTTG GCTGGTTTCG TTGTTGTTTC TTATTCTGGT
 3781 TGGAACAGAA GATTGACTGT TGAAGATATT GAAGTTGCTC CAGAACATAG AGGTCATGGT
 3841 GTTGGTAGAG CTTTGATGGG TTTGGCTACT GAATTCGCCA GAGAAAGAGG TGCTGGTCAT
 3901 TTGTGGTTGG AAGTTACCAA TGTTAATGCT CCAGCTATTC ATGCTTATAG AAGAATGGGT
 3961 TTCACTTTGT GTGGTTTGGA TACTGCTTTA TACGATGGTA CTGCTTCCGA TGGTGAACAA
 4021 GCTTTGTATA TGTCTATGCC ATGTCCATAA taacTCGACc agtactgaca ataaaaagat
 4081 tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta
 4141 atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa
 4201 gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact
 4261 gctgtcgatt cgatactaac gccgccatcc agtgTCGAtc gacGAAGTTC CTATACTTTC
 4321 TAGAGAATAG GAACTTCGTC ctcgaggggg ggcccggtac cagcttttgt tccctttagt
 4381 gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
 4441 atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg
 4501 cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
 4561 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
 4621 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
 4681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
 4741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggcCG
 4801 CGTTGCTGGC GTTTTTCCAT AGGCTCGGCC CCCCTGACGA GCATCACAAA AATCGACGCT
 4861 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTC CCCCCTGGAA
 4921 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
 4981 TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT
 5041 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
 5101 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
 5161 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
 5221 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC
 5281 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG
 5341 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC
 5401 AAGAAGATCC TTTGATCTtt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
 5461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
 5521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agTTACCAAT
 5581 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT
 5641 GACTGCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG
 5701 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG
 5761 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA
 5821 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG
 5881 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG
 5941 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGAAAAAAA GCGGTTAGCT
 6001 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA
 6061 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG
 6121 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC
 6181 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG
 6241 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA
 6301 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG
 6361 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT
 6421 GTTGAATACT CATactcttc ctttttcaat attattgaag catttatcag ggttattgtc
 6481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
 6541 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
 6601 ataaaaatag gcgtatcacg aggccctttc gtc
//

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