| 出品人: | Irene Castano Navarro（2015） |
|---|---|
| 载体名称: | pYC44 |
| 质粒类型: | 酿酒酵母表达载体；Initial Integrative Vector；FLP/FRT系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 5913 bp |
| 5' 测序引物及序列: | M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’ |
| 3' 测序引物及序列: | -- |
| 载体标签: | FRT::3'UTRcta::NAT::FRT |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | URA3，Nourseothricin |
| 克隆菌株: | DH10B感受态细胞 ,30°C |
| 宿主细胞（系）: | 出芽酵母（酿酒酵母）、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | #63903 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pYC44载体质粒基本信息
pYC44质粒图谱载体图谱和pYC44载体序列质粒序列多克隆位点信息
pYC44质粒载体简介
pYC44质粒序列载体序列
LOCUS pYC44 5913 bp DNA SYN
DEFINITION pYC44
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5913
/organism="pYC44"
/mol_type="other DNA"
misc_feature complement(29..51)
/label="pGEX_3_primer"
promoter 196..409
/label="URA3_promoter"
gene 411..1211
/label="URA3"
/gene="URA3"
CDS 411..1214
/label="ORF frame 3"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
CDS complement(446..922)
/label="ORF frame 3"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
misc_feature complement(1814..1962)
/label="lacZ_a"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
misc_feature 1928..1950
/label="M13_pUC_fwd_primer"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
promoter 1943..1959
/label="M13_forward20_primer"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
promoter 1966..1984
/label="T7_promoter"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
misc_feature 2017..2033
/label="pBluescriptSK_primer"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
CDS complement(2701..3222)
/label="ORF frame 1"
/translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF
NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW
INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRMI
RLFMFGCDVRTVS*"
CDS 2758..3330
/label="ORF frame 1"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
promoter complement(3652..3671)
/label="T3_promoter"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
promoter complement(3688..3706)
/label="M13_reverse_primer"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
misc_feature complement(3705..3727)
/label="M13_pUC_rev_primer"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
rep_origin complement(4079..4698)
/label="pBR322_origin"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
gene complement(4853..5713)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
CDS complement(4853..5713)
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5755..5783)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG
421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA
1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg
1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta
1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atccgaagtt
2041 cctatacttt ctagagaata ggaacttcgt gcgcttttga accacgtaaa gtgctgtaaa
2101 Ccagctctat tcaatgaggt aaatgataaa gtttataaga gaccaaaaaa ttcaaaaaaa
2161 aaattgaaaa attatattgg tatttatatg aaacaatagt tcagttcaaa gcgctagctt
2221 ttctcatctc aactaaccgg tattaggtgg gtatttattt aatatgggaa caagaaatgt
2281 gacaTtaatg ttcatgtttt tattttcatt atttattcta tatggtttgg gacttgatgt
2341 tatgtcttaa ttcatagaGA TCCgcttgcc tcgtccccgc cgggtcaccc ggccagcgac
2401 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
2461 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
2521 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag
2581 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
2641 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg
2701 ctaggataca gttctcacat cacatccgaa cataaacaac cgGATCatcc tacaattATG
2761 TCTACTACTT TGGATGATAC TGCTTATAGA TACAGAACTT CTGTTCCAGG TGATGCTGAA
2821 GCTATTGAAG CTTTGGATGG TTCTTTCACT ACCGATACTG TTTTTAGAGT TACTGCTACT
2881 GGTGATGGTT TCACTTTGAG AGAAGTTCCA GTTGATCCAC CATTGACTAA GGTTTTCCCA
2941 GATGATGAAT CCGATGATGA ATCCGATGCT GGTGAAGATG GTGATCCAGA TTCTAGAACT
3001 TTCGTTGCTT ATGGTGATGA TGGTGATTTG GCTGGTTTCG TTGTTGTTTC TTATTCTGGT
3061 TGGAACAGAA GATTGACTGT TGAAGATATT GAAGTTGCTC CAGAACATAG AGGTCATGGT
3121 GTTGGTAGAG CTTTGATGGG TTTGGCTACT GAATTCGCCA GAGAAAGAGG TGCTGGTCAT
3181 TTGTGGTTGG AAGTTACCAA TGTTAATGCT CCAGCTATTC ATGCTTATAG AAGAATGGGT
3241 TTCACTTTGT GTGGTTTGGA TACTGCTTTA TACGATGGTA CTGCTTCCGA TGGTGAACAA
3301 GCTTTGTATA TGTCTATGCC ATGTCCATAA taacTCGACc agtactgaca ataaaaagat
3361 tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta
3421 atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa
3481 gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact
3541 gctgtcgatt cgatactaac gccgccatcc agtgTCGAtc gacGAAGTTC CTATACTTTC
3601 TAGAGAATAG GAACTTCGTC ctcgaggggg ggcccggtac cagcttttgt tccctttagt
3661 gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
3721 atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg
3781 cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
3841 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
3901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
3961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
4021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggcCG
4081 CGTTGCTGGC GTTTTTCCAT AGGCTCGGCC CCCCTGACGA GCATCACAAA AATCGACGCT
4141 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTC CCCCCTGGAA
4201 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
4261 TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT
4321 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
4381 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
4441 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
4501 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC
4561 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG
4621 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC
4681 AAGAAGATCC TTTGATCTtt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
4741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
4801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agTTACCAAT
4861 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT
4921 GACTGCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG
4981 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG
5041 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA
5101 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG
5161 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG
5221 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGAAAAAAA GCGGTTAGCT
5281 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA
5341 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG
5401 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC
5461 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG
5521 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA
5581 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG
5641 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT
5701 GTTGAATACT CATactcttc ctttttcaat attattgaag catttatcag ggttattgtc
5761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
5821 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
5881 ataaaaatag gcgtatcacg aggccctttc gtc
//
