出品人: | Irene Castano Navarro(2015) |
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载体名称: | pYC44 |
质粒类型: | 酿酒酵母表达载体;Initial Integrative Vector;FLP/FRT系统载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | -- |
载体大小: | 5913 bp |
5' 测序引物及序列: | M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’ |
3' 测序引物及序列: | -- |
载体标签: | FRT::3'UTRcta::NAT::FRT |
载体抗性: | 氨苄青霉素 |
筛选标记: | URA3,Nourseothricin |
克隆菌株: | DH10B感受态细胞 ,30°C |
宿主细胞(系): | 出芽酵母(酿酒酵母)、白色念珠菌 |
备注: | -- |
产品目录号: | #63903 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pYC44
价格:1200元
联系方式:I47-825O-882O
买家导航
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pYC44 5913 bp DNA SYN DEFINITION pYC44 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5913 /organism="pYC44" /mol_type="other DNA" misc_feature complement(29..51) /label="pGEX_3_primer" promoter 196..409 /label="URA3_promoter" gene 411..1211 /label="URA3" /gene="URA3" CDS 411..1214 /label="ORF frame 3" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*" CDS complement(446..922) /label="ORF frame 3" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" misc_feature complement(1814..1962) /label="lacZ_a" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" misc_feature 1928..1950 /label="M13_pUC_fwd_primer" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" promoter 1943..1959 /label="M13_forward20_primer" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" promoter 1966..1984 /label="T7_promoter" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" misc_feature 2017..2033 /label="pBluescriptSK_primer" /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*" CDS complement(2701..3222) /label="ORF frame 1" /translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRMI RLFMFGCDVRTVS*" CDS 2758..3330 /label="ORF frame 1" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" promoter complement(3652..3671) /label="T3_promoter" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" promoter complement(3688..3706) /label="M13_reverse_primer" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" misc_feature complement(3705..3727) /label="M13_pUC_rev_primer" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" rep_origin complement(4079..4698) /label="pBR322_origin" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" gene complement(4853..5713) /label="Ampicillin" /gene="Ampicillin" /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM GFTLCGLDTALYDGTASDGEQALYMSMPCP*" CDS complement(4853..5713) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(5755..5783) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG 421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA 481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT 541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA 601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT 661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC 721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG 781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC 841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT 901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG 961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG 1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG 1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG 1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG 1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt 1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta 1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA 1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC 1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA 1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG 1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG 1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg 1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG 1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA 1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta 1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atccgaagtt 2041 cctatacttt ctagagaata ggaacttcgt gcgcttttga accacgtaaa gtgctgtaaa 2101 Ccagctctat tcaatgaggt aaatgataaa gtttataaga gaccaaaaaa ttcaaaaaaa 2161 aaattgaaaa attatattgg tatttatatg aaacaatagt tcagttcaaa gcgctagctt 2221 ttctcatctc aactaaccgg tattaggtgg gtatttattt aatatgggaa caagaaatgt 2281 gacaTtaatg ttcatgtttt tattttcatt atttattcta tatggtttgg gacttgatgt 2341 tatgtcttaa ttcatagaGA TCCgcttgcc tcgtccccgc cgggtcaccc ggccagcgac 2401 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt 2461 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat 2521 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag 2581 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat 2641 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg 2701 ctaggataca gttctcacat cacatccgaa cataaacaac cgGATCatcc tacaattATG 2761 TCTACTACTT TGGATGATAC TGCTTATAGA TACAGAACTT CTGTTCCAGG TGATGCTGAA 2821 GCTATTGAAG CTTTGGATGG TTCTTTCACT ACCGATACTG TTTTTAGAGT TACTGCTACT 2881 GGTGATGGTT TCACTTTGAG AGAAGTTCCA GTTGATCCAC CATTGACTAA GGTTTTCCCA 2941 GATGATGAAT CCGATGATGA ATCCGATGCT GGTGAAGATG GTGATCCAGA TTCTAGAACT 3001 TTCGTTGCTT ATGGTGATGA TGGTGATTTG GCTGGTTTCG TTGTTGTTTC TTATTCTGGT 3061 TGGAACAGAA GATTGACTGT TGAAGATATT GAAGTTGCTC CAGAACATAG AGGTCATGGT 3121 GTTGGTAGAG CTTTGATGGG TTTGGCTACT GAATTCGCCA GAGAAAGAGG TGCTGGTCAT 3181 TTGTGGTTGG AAGTTACCAA TGTTAATGCT CCAGCTATTC ATGCTTATAG AAGAATGGGT 3241 TTCACTTTGT GTGGTTTGGA TACTGCTTTA TACGATGGTA CTGCTTCCGA TGGTGAACAA 3301 GCTTTGTATA TGTCTATGCC ATGTCCATAA taacTCGACc agtactgaca ataaaaagat 3361 tcttgttttc aagaacttgt catttgtata gtttttttat attgtagttg ttctatttta 3421 atcaaatgtt agcgtgattt atattttttt tcgcctcgac atcatctgcc cagatgcgaa 3481 gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg tgaatgctgg tcgctatact 3541 gctgtcgatt cgatactaac gccgccatcc agtgTCGAtc gacGAAGTTC CTATACTTTC 3601 TAGAGAATAG GAACTTCGTC ctcgaggggg ggcccggtac cagcttttgt tccctttagt 3661 gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 3721 atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg 3781 cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 3841 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 3901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 3961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 4021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggcCG 4081 CGTTGCTGGC GTTTTTCCAT AGGCTCGGCC CCCCTGACGA GCATCACAAA AATCGACGCT 4141 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTC CCCCCTGGAA 4201 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 4261 TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT 4321 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 4381 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 4441 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 4501 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 4561 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 4621 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 4681 AAGAAGATCC TTTGATCTtt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 4741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 4801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agTTACCAAT 4861 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 4921 GACTGCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 4981 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 5041 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 5101 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 5161 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 5221 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGAAAAAAA GCGGTTAGCT 5281 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 5341 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 5401 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 5461 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 5521 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 5581 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 5641 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 5701 GTTGAATACT CATactcttc ctttttcaat attattgaag catttatcag ggttattgtc 5761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 5821 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 5881 ataaaaatag gcgtatcacg aggccctttc gtc //