质粒名称: | pHDM-Tat1b |
---|---|
出品公司: | Addgene |
目录编号: | 164442 |
存储实验室: | Alejandro Balazs |
载体骨架基本信息 |
|
载体名称: | HDM |
载体出品公司: | -- |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
载体抗性: | 氨苄霉素100 μg/mL |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
|
插入基因名称: | HIV-1 Tat |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子: | -- |
标签/融合蛋白: | -- |
克隆方法: | -- |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
|
材料和方法部分: | pHDM-Tat1b was a gift from Alejandro Balazs (Addgene plasmid # 164442 ; http://n2t.net/addgene:164442 ; RRID:Addgene_164442) |
参考文献部分: | COVID-19-neutralizing antibodies predict disease severity and survival. Garcia-Beltran WF, Lam EC, Astudillo MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter G, Charles RC, Dighe A, Branda JA, Lennerz JK, Lingwood D, Schmidt AG, Iafrate AJ, Balazs AB. Cell. 2021 Jan 21;184(2):476-488.e11. doi: 10.1016/j.cell.2020.12.015. Epub 2020 Dec 15. 10.1016/j.cell.2020.12.015 PubMed 33412089 |
买家导航
pHDMTat1b载体基本信息
pHDMTat1b载体图谱质粒图谱和多克隆位点信息
pHDMTat1b载体简介
pHDMTat1b载体序列
LOCUS pHDM-Tat1b 4829 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Encodes HIV-1 Tat accessory protein for pseudovirus production. ACCESSION . VERSION . KEYWORDS pHDM-Tat1b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4829) TITLE COVID-19 neutralizing antibodies predict disease severity and survival JOURNAL Unpublished REFERENCE 2 (bases 1 to 4829) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4829 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(523..539) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(524..540) /label=pBluescriptKS /note="For pBluescript vector" polyA_signal 633..1027 /label=-beta-globin poly(A) /note="human beta-globin polyadenylation signal" primer_bind complement(1361..1379) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1447..1551 /gene="bla" /label=AmpR promoter CDS 1552..2412 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1770..1789) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2583..3171 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3072..3091 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind complement(3714..3733) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" enhancer 3835..4214 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4215..4418 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4369..4389 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4415..4439 /label=LNCX /note="Human CMV promoter, forward primer" intron 4532..178 /label=-beta-globin intron /note="internally truncated intron from human beta-globin" ORIGIN 1 tataaatatt tctgcatata aattgtaact gatgtaagag gtttcatatt gctaatagca 61 gctacaatcc agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct 121 gagtccaagc taggcccttt tgctaatcat gttcatacct cttatcttcc tcccacagct 181 cctgggcaac gtgctggtct gtgtgctggc ccatcacttt ggcaaagaat tccgcgggcg 241 gccgcgaaat ggagccagta gatcctagac tagagccctg gaagcatcca ggaagtcagc 301 ctaaaactgc ttgtaccact tgctattgta aaaagtgttg ctttcattgc caagtttgtt 361 tcacaacaaa agccttaggc atctcctatg gcaggaagaa gcggagacag cgacgaagac 421 ctcctcaagg cagtcagact catcaagttt ctctatcaaa gcaacccacc tcccaacccc 481 gaggggaccc gacaggcccg aaggaatagg atccaagctt atcgataccg tcgacctcga 541 gggcccagat ctaattcacc ccaccagtgc aggctgccta tcagaaagtg gtggctggtg 601 tggctaatgc cctggcccac aagtatcact aagctcgctt tcttgctgtc caatttctat 661 taaaggttcc tttgttccct aagtccaact actaaactgg gggatattat gaagggcctt 721 gagcatctgg attctgccta ataaaaaaca tttattttca ttgcaatgat gtatttaaat 781 tatttctgaa tattttacta aaaagggaat gtgggaggtc agtgcattta aaacataaag 841 aaatgaagag ctagttcaaa ccttgggaaa atacactata tcttaaactc catgaaagaa 901 ggtgaggctg caaacagcta atgcacattg gcaacagccc ctgatgccta tgccttattc 961 atccctcaga aaaggattca agtagaggct tgatttggag gttaaagttt tgctatgctg 1021 tattttacat tacttattgt tttagctgtc ctcatgaatg tcttttcact acccatttgc 1081 ttatcctgca tctctcagcc ttgactccac tcagttctct tgcttagaga taccaccttt 1141 cccctgaagt gttccttcca tgttttacgg cgagatggtt tctcctcgcc tggccactca 1201 gccttagttg tctctgttgt cttatagagg tctacttgaa gaaggaaaaa cagggggcat 1261 ggtttgactg tcctgtgagc ccttcttccc tgcctccccc actcacagtg acccggaatc 1321 cctcgacatg gcagtctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt 1381 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 1441 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 1501 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 1561 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 1621 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 1681 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 1741 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 1801 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 1861 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 1921 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 1981 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 2041 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 2101 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 2161 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 2221 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 2281 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 2341 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 2401 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 2461 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 2521 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 2581 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 2641 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 2701 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 2761 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 2821 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 2881 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 2941 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 3001 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 3061 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 3121 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 3181 aacggagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca 3241 agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt 3301 ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat 3361 gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc 3421 aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg 3481 atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca 3541 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga 3601 agaatcataa tggggaaggc catccagcct cgcgtcgggg agctttttgc aaaagcctag 3661 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc 3721 ctctgcataa ataaaaaaaa ttagtcagcc atgagcttgg cccattgcat acgttgtatc 3781 catatcataa tatgtacatt tatattggct catgtccaac attaccgcca tgttgacatt 3841 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 3901 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 3961 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 4021 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 4081 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 4141 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 4201 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 4261 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 4321 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 4381 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg 4441 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc 4501 tcccctcgaa gctgatcctg agaacttcag ggtgagtcta tgggaccctt gatgttttct 4561 ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta acagggtaca 4621 catattgacc aaatcagggt aattttgcat ttgtaatttt aaaaaatgct ttcttctttt 4681 aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc tttcagggca 4741 ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag tgataatttc 4801 tgggttaagg caatagcaat atttctgca //
pHDMTat1b载体图谱质粒图谱pdf版和相关资料下载
pHDMTat1b载体质粒应用举例
- 上一篇:DCT.VV.Orf42
- 下一篇:pLVX-Tet3G blasticidin