CMV-T7-hOsa2

价格:1500元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: CMV-T7-hOsa2 
出品公司: Addgene
目录编号: 17987
存储实验室: Naoko Tanese

载体骨架基本信息
载体名称: pCGT
载体出品公司: --
载体抗性: 氨苄青霉素
空载体大小: --
完整质粒大小: --
载体修饰: --
载体类型: 哺乳细胞表达
筛选标记: --
高拷贝/低拷贝: 未知
生长条件: 37 ℃

插入基因信息
插入基因名称: Osa2,BAF250b
物种来源: H. sapiens (human)
插入大小: 5500 bp
基因突变: --
GenBank编号 AF521671
启动子 --
标签/融合蛋白: T7表位(主干N端)
克隆方法: 限制性内切酶
5' 克隆位点: Xba I(未销毁)
3' 克隆位点: BgI I I/BamH I(在克隆过程中被销毁)
5' 测序引物 CMV-F
3' 测序引物 --

文献引用方法
材料和方法部分: CMV-T7-hOsa2 was a gift from Naoko Tanese (Addgene plasmid # 17987 ; http://n2t.net/addgene:17987 ; RRID:Addgene_17987)
参考文献部分: Largest subunits of the human SWI/SNF chromatin-remodeling complex promote transcriptional activation by steroid hormone receptors. Inoue H, Furukawa T, Giannakopoulos S, Zhou S, King DS, Tanese N. J Biol Chem. 2002 Nov 1. 277(44):41674-85. 10.1074/jbc.M205961200 PubMed 12200431

载体图谱质粒图谱和多克隆位点信息

CMV-T7-hOsa2质粒图谱

载体简介

载体序列

LOCUS       CMV-T7-hOsa2               10561 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    CMV-T7-hOsa2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10561)
  AUTHORS   Inoue H, Furukawa T, Giannakopoulos S, Zhou S, King DS, Tanese N
  TITLE     Largest subunits of the human SWI/SNF chromatin-remodeling complex 
            promote transcriptional activation by steroid hormone receptors.
  JOURNAL   J Biol Chem. 2002 Nov 1. 277(44):41674-85.
  PUBMED    12200431
REFERENCE   2  (bases 1 to 10561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10561
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(30..52)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     152..171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(238..693)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(375..396)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     587..606
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     819..841
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     833..850
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     834..850
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     857..876
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        857..875
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        894..1197
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1198..1401
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1352..1372
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1398..1422
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           1491..1530
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     intron          7153..7725
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(7857..7876)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_site      7922..7977
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(7976..7995)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      8331..8466
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8385..8404)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8572..8589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8743..9331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8823..8842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9502..10362)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10125..10144
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10363..10467)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10535..10553
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
      241 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
      301 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
      361 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
      421 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
      481 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
      541 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
      601 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
      661 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
      721 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      781 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      841 gacggccagt gaattgtaat acgactcact atagggcgaa ttcgagctcg ccccgttaca
      901 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      961 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     1021 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     1081 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     1141 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     1201 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     1261 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     1321 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     1381 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     1441 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgggg gatcttggtg
     1501 gcgtgaaact cccgcacctc ttcggccagc gccttgtaga agcgcgtatg gcttctagga
     1561 tggcatcgat gacaggtggc caacagatgg gttctagagc ctgggcggcc gcgcaacaaa
     1621 ggagtcaccc ggcgatgagc cccggcaccc ccggaccgac catgggcaga tcccagggca
     1681 gcccaatgga tccaatggtg atgaagagac ctcagttgta tggcatgggc agtaaccctc
     1741 attctcagcc tcagcagagc agtccgtacc caggaggttc ctatggccct ccaggcccac
     1801 agcggtatcc aattggcatc cagggtcgga ctcccggggc catggccgga atgcagtacc
     1861 ctcagcagca gatgccacct cagtatggac agcaaggtgt gagtggttac tgccagcagg
     1921 gccaacagcc atattacagc cagcagccgc agcccccgca cctcccaccc caggcgcagt
     1981 atctgccgtc ccagtcccag cagaggtacc agccgcagca ggacatgtct caggaaggct
     2041 atggaactag atctcaacct cctctggccc ccggaaaacc taaccatgaa gacttgaact
     2101 taatacagca agaaagacca tcaagtttac cagatctgtc tggctccatt gatgacctcc
     2161 ccacgggaac ggaagcaact ttgagctcag cagtcagtgc atccgggtcc acgagcagcc
     2221 aaggggatca gagcaacccg gcgcagtcgc ctttctcccc acatgcgtcc cctcatctct
     2281 ccagcatccc ggggggccca tctccctctc ctgttggctc tcctgtagga agcaaccagt
     2341 ctcgatctgg cccaatctct cctgcaagta tcccaggtag tcagatgcct ccgcagccac
     2401 ccgggagcca gtcagaatcc agttcccatc ccgccttgag ccagtcacca atgccacagg
     2461 aaagaggttt tatggcaggc acacaaagaa accctcagat ggctcagtat ggacctcaac
     2521 agacaggacc atccatgtcg cctcatcctt ctcctggggg ccagatgcat gctggaatca
     2581 gtagctttca gcagagtaac tcaagtggga cttacggtcc acagatgagc cagtatggac
     2641 cacaaggtaa ctactccaga cccccagcgt atagtggggt gcccagtgca agctacagcg
     2701 gcccagggcc cggtatgggt atcagtgcca acaaccagat gcatggacaa gggccaagcc
     2761 agccatgtgg tgctgtgccc ctgggacgaa tgccatcagc tgggatgcag aacagaccat
     2821 ttcctggaaa tatgagcagc atgaccccca gttctcctgg catgtctcag cagggagggc
     2881 caggaatggg gccgccaatg ccaactgtga accgtaaggc acaggaggca gccgcagcag
     2941 tgatgcaggc tgctgcgaac tcagcacaaa gcaggcaagg cagtttcccc ggcatgaacc
     3001 agagtggact tatggcttcc agctctccct acagccagcc catgaacaac agctctagcc
     3061 tgatgaacac gcaggcgccg ccctacagca tggcgcccgc catggtgaac agctcggcag
     3121 catctgtggg tcttgcagat atgatgtctc ctggtgaatc caaactgccc ctgcctctca
     3181 aagcagacgg caaagaagaa ggcactccac agcccgagag caagtcaaag aagtccagct
     3241 cctccaccac tactggggag aagatcacga aggtgtacga gctggggaat gagccagaga
     3301 gaaagctctg ggtcgaccga tacctcacct tcatggaaga gagaggctct cctgtctcaa
     3361 gtctgcctgc cgtgggcaag aagcccctgg acctgttccg actctacgtc tgcgtcaaag
     3421 agatcggggg tttggcccag gttaataaaa acaagaagtg gcgtgagctg gcaaccaacc
     3481 taaacgttgg cacctcaagc agtgcagcga gctccctgaa aaagcagtat attcagtacc
     3541 tgtttgcctt tgagtgcaag atcgaacgtg gggaggagcc cccgccggaa gtcttcagca
     3601 ccggggacac caaaaagcag cccaagctcc agccgccatc tcctgctaac tcgggatcct
     3661 tgcaaggccc acagaccccc cagtcaactg gcagcaattc catggcagag gttccaggtg
     3721 acctgaagcc acctacccca gcctccaccc ctcacggcca gatgactcca atgcaaggtg
     3781 gaagaagcag tacaatcagt gtgcacgacc cattctcaga tgtgagtgat tcatccttcc
     3841 cgaaacggaa ctccatgact ccaaacgccc cctaccagca gggcatgagc atgcccgatg
     3901 tgatgggcag gatgccctat gagcccaaca aggacccctt tgggggaatg agaaaagtgc
     3961 ctggaagcag cgagcccttt atgacgcaag gacagatgcc caacagcagc atgcaggaca
     4021 tgtacaacca aagtccctcc ggagcaatgt ctaacctggg catggggcag cgccagcagt
     4081 ttccctatgg agccagttac gaccgaaggc atgaacctta tgggcagcag tatccaggcc
     4141 aaggccctcc ctcgggacag ccgccgtatg gagggcacca gcccggcctg tacccacagc
     4201 agccgaatta caaacgccat atggacggca tgtacgggcc cccagccaag cgccacgagg
     4261 gcgacatgta caacatgcag tacagcagcc agcagcagga gatgtacaac cagtatggag
     4321 gctcctactc gggcccggac cgcaggccca tccagggcca gtacccgtat ccctacagca
     4381 gggagaggat gcagggcccg gggcagatcc agacacacgg aatcccgcct cagatgatgg
     4441 gcggcccgct gcagtcgtcc tccagtgagg ggcctcagca gaatatgtgg gcagcacgca
     4501 atgatatgcc ttatccctac cagaacaggc agggccctgg cggccctaca caggcgcccc
     4561 cttacccagg catgaaccgc acagacgata tgatggtacc cgatcagagg ataaatcatg
     4621 agagccagtg gccttctcac gtcagccagc gtcagcctta tatgtcgtcc tcagcctcca
     4681 tgcagcccat cacacgccca ccacagccgt cctaccagac gccaccgtca ctgccaaatc
     4741 acatctccag ggcgcccagc ccagcgtcct tccagcgctc cctggagaac cgcatgtctc
     4801 caagcaagtc tccttttctg ccgtctatga agatgcagaa ggtcatgccc acggtcccca
     4861 catcccaggt caccgggcca ccaccccaac cacccccaat cagaagggag atcacctttc
     4921 ctcctggctc agtagaagca tcacaaccag tcttgaaaca aaggcgaaag attacctcca
     4981 aagatatcgt tactcctgag gcgtggcgtg tgatgatgtc ccttaaatca ggtcttttgg
     5041 ctgagagtac gtgggctttg gacactatta atattcttct gtatgatgac agcactgttg
     5101 ctactttcaa tctctcccag ttgtctggat ttctcgaact tttagtcgag tactttagaa
     5161 aatgcctgat tgacattttt ggaattctta tggaatatga agtgggagac cccagccaaa
     5221 aagcacttga tcacaacgca gcaaggaagg atgacagcca gtccttggca gacgattctg
     5281 ggaaagagga ggaagatgct gaatgtattg atgacgacga ggaagacgag gaggatgagg
     5341 aggaagacag cgagaagaca gaaagcgatg aaaagagcag catcgctctg actgccccgg
     5401 acgccgctgc agacccaaag gagaagccca agcaagccag taagttcgac aagctgccaa
     5461 taaagatagt caaaaagaac aacctgtttg ttgttgaccg atctgacaag ttggggcgtg
     5521 tgcaggagtt caatagtggc cttctgcact ggcagctcgg cgggggtgac accaccgagc
     5581 acattcagac tcactttgag agcaagatgg aaattcctcc tcgcaggcgc ccacctcccc
     5641 ccttaagctc cgcaggtaga aagaaagagc aagaaggcaa aggcgactct gaagagcagc
     5701 aagagaaaag catcatagca accatcgatg acgtcctctc tgctcggcca ggggcattgc
     5761 ctgaagacgc aaaccctggg ccccagaccg aaagcagtaa gtttcccttt ggtatccagc
     5821 aagccaaaag tcaccggaac atcaagctgc tggaggacga gcccaggagc cgagacgaga
     5881 ctcctctgtg taccatcgcg cactggcagg actcgctggc taagcgatgc atctgtgtgt
     5941 ccaatattgt ccgtagcttg tcattcgtgc ctggcaatga tgccgaaatg tccaaacatc
     6001 caggcctggt gctgatcctg gggaagctga ttcttcttca ccacgagcat ccagagagaa
     6061 agcgagcacc gcagacctat gagaaagagg aggatgagga caagggggtg gcctgcagca
     6121 aagatgagtg gtggtgggac tgcctcgagg tcttgaggga taacacgttg gtcacgttgg
     6181 ccaacatttc cgggcagcta gacttgtctg cttacacgga aagcatctgc ttgccaattt
     6241 tggatggctt gctgcactgg atggtgtgcc cgtctgcaga ggcacaagat ccctttccaa
     6301 ctgtgggacc caactcggtc ctgtcgcctc agagacttgt gctggagacc ctctgtaaac
     6361 tcagtatcca ggacaataat gtggacctga tcttggccac tcctccattt agtcgtcagg
     6421 agaaattcta tgctacatta gttaggtacg ttggggatcg caaaaaccca gtctgtcgag
     6481 aaatgtccat ggcgctttta tcgaaccttg cccaagggga cgcactagca gcaagggcca
     6541 tagctgtgca gaaaggaagc attggaaact tgataagctt cctagaggat ggggtcacga
     6601 tggcccagta ccagcagagc cagcacaacc tcatgcacat gcagcccccg cccctggaac
     6661 cacctagcgt agacatgatg tgcagggcgg ccaaggcttt gctagccatg gccagagtgg
     6721 acgaaaaccg ctcggaattc cttttgcacg agggccggtt gctggatatc tcgatatcag
     6781 ctgtcctgaa ctctctggtt gcatctgtca tctgtgatgt actgtttcag attgggcagt
     6841 tatgacataa gtgagaaggc aagcatgtgt gagtgaagat tagagggtca catataactg
     6901 gctgttttct gttcttgttt atccagcgta ggaagaagga aaagaaaatc tttgctcctc
     6961 tgccccattc actatttacc aattgggaat taaagaaata attaatttga acagttatga
     7021 aattaatatt tgctgtctgt gtgtataagt acatcctttg gggttttttt tttctctttt
     7081 ttttaaccaa agttgctgtc tagtgcattc aaaggtcact ttttgttctt cacagatcct
     7141 gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa
     7201 ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc
     7261 ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa
     7321 ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg
     7381 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc
     7441 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag
     7501 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg
     7561 cgtggaaata ttcttattgg tagaaacaac tacaccctgg tcatcatcct gcctttctct
     7621 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg
     7681 ggcccctctg ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg
     7741 ctggttgttg tgctgtctca tcattttggc aaagaattca ctcctcaggt gcaggctgcc
     7801 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     7861 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     7921 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     7981 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     8041 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     8101 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     8161 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     8221 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     8281 gtccctcttc tcttatggag atccctcgag gagctttttg caaaagccta ggcctccaaa
     8341 aaagcctctt cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     8401 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     8461 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgctgc attaatgaat
     8521 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8581 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8641 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8701 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8761 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8821 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8881 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8941 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9001 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9061 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9121 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9181 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9241 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9301 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9361 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9421 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9481 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9541 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9601 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9661 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9721 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9781 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9841 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9901 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9961 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10021 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10081 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10141 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10201 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10261 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10321 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10381 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10441 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10501 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10561 t
//

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