质粒名称: | CMV-T7-hOsa2 |
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出品公司: | Addgene |
目录编号: | 17987 |
存储实验室: | Naoko Tanese |
载体骨架基本信息 |
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载体名称: | pCGT |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Osa2,BAF250b |
物种来源: | H. sapiens (human) |
插入大小: | 5500 bp |
基因突变: | -- |
GenBank编号 | AF521671 |
启动子 | -- |
标签/融合蛋白: | T7表位(主干N端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | Xba I(未销毁) |
3' 克隆位点: | BgI I I/BamH I(在克隆过程中被销毁) |
5' 测序引物 | CMV-F |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | CMV-T7-hOsa2 was a gift from Naoko Tanese (Addgene plasmid # 17987 ; http://n2t.net/addgene:17987 ; RRID:Addgene_17987) |
参考文献部分: | Largest subunits of the human SWI/SNF chromatin-remodeling complex promote transcriptional activation by steroid hormone receptors. Inoue H, Furukawa T, Giannakopoulos S, Zhou S, King DS, Tanese N. J Biol Chem. 2002 Nov 1. 277(44):41674-85. 10.1074/jbc.M205961200 PubMed 12200431 |
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载体基本信息
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载体简介
载体序列
LOCUS CMV-T7-hOsa2 10561 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS CMV-T7-hOsa2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10561) AUTHORS Inoue H, Furukawa T, Giannakopoulos S, Zhou S, King DS, Tanese N TITLE Largest subunits of the human SWI/SNF chromatin-remodeling complex promote transcriptional activation by steroid hormone receptors. JOURNAL J Biol Chem. 2002 Nov 1. 277(44):41674-85. PUBMED 12200431 REFERENCE 2 (bases 1 to 10561) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10561 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(30..52) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 152..171 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin complement(238..693) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(375..396) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 587..606 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 819..841 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 833..850 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 834..850 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 857..876 /label=T7 /note="T7 promoter, forward primer" promoter 857..875 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" enhancer 894..1197 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1198..1401 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 1352..1372 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 1398..1422 /label=LNCX /note="Human CMV promoter, forward primer" 5'UTR 1491..1530 /label=HSV TK 5'-UTR /note="5' untranslated region from the herpes simplex virus thymidine kinase gene" intron 7153..7725 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" primer_bind complement(7857..7876) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_site 7922..7977 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal" primer_bind complement(7976..7995) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" rep_origin 8331..8466 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(8385..8404) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(8572..8589) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8743..9331) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8823..8842) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9502..10362) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10125..10144 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10363..10467) /gene="bla" /label=AmpR promoter primer_bind 10535..10553 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg 181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa 241 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 301 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 361 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 421 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 481 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 541 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 601 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 661 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg 721 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 781 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 841 gacggccagt gaattgtaat acgactcact atagggcgaa ttcgagctcg ccccgttaca 901 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 961 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 1021 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 1081 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 1141 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 1201 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 1261 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 1321 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 1381 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca 1441 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgggg gatcttggtg 1501 gcgtgaaact cccgcacctc ttcggccagc gccttgtaga agcgcgtatg gcttctagga 1561 tggcatcgat gacaggtggc caacagatgg gttctagagc ctgggcggcc gcgcaacaaa 1621 ggagtcaccc ggcgatgagc cccggcaccc ccggaccgac catgggcaga tcccagggca 1681 gcccaatgga tccaatggtg atgaagagac ctcagttgta tggcatgggc agtaaccctc 1741 attctcagcc tcagcagagc agtccgtacc caggaggttc ctatggccct ccaggcccac 1801 agcggtatcc aattggcatc cagggtcgga ctcccggggc catggccgga atgcagtacc 1861 ctcagcagca gatgccacct cagtatggac agcaaggtgt gagtggttac tgccagcagg 1921 gccaacagcc atattacagc cagcagccgc agcccccgca cctcccaccc caggcgcagt 1981 atctgccgtc ccagtcccag cagaggtacc agccgcagca ggacatgtct caggaaggct 2041 atggaactag atctcaacct cctctggccc ccggaaaacc taaccatgaa gacttgaact 2101 taatacagca agaaagacca tcaagtttac cagatctgtc tggctccatt gatgacctcc 2161 ccacgggaac ggaagcaact ttgagctcag cagtcagtgc atccgggtcc acgagcagcc 2221 aaggggatca gagcaacccg gcgcagtcgc ctttctcccc acatgcgtcc cctcatctct 2281 ccagcatccc ggggggccca tctccctctc ctgttggctc tcctgtagga agcaaccagt 2341 ctcgatctgg cccaatctct cctgcaagta tcccaggtag tcagatgcct ccgcagccac 2401 ccgggagcca gtcagaatcc agttcccatc ccgccttgag ccagtcacca atgccacagg 2461 aaagaggttt tatggcaggc acacaaagaa accctcagat ggctcagtat ggacctcaac 2521 agacaggacc atccatgtcg cctcatcctt ctcctggggg ccagatgcat gctggaatca 2581 gtagctttca gcagagtaac tcaagtggga cttacggtcc acagatgagc cagtatggac 2641 cacaaggtaa ctactccaga cccccagcgt atagtggggt gcccagtgca agctacagcg 2701 gcccagggcc cggtatgggt atcagtgcca acaaccagat gcatggacaa gggccaagcc 2761 agccatgtgg tgctgtgccc ctgggacgaa tgccatcagc tgggatgcag aacagaccat 2821 ttcctggaaa tatgagcagc atgaccccca gttctcctgg catgtctcag cagggagggc 2881 caggaatggg gccgccaatg ccaactgtga accgtaaggc acaggaggca gccgcagcag 2941 tgatgcaggc tgctgcgaac tcagcacaaa gcaggcaagg cagtttcccc ggcatgaacc 3001 agagtggact tatggcttcc agctctccct acagccagcc catgaacaac agctctagcc 3061 tgatgaacac gcaggcgccg ccctacagca tggcgcccgc catggtgaac agctcggcag 3121 catctgtggg tcttgcagat atgatgtctc ctggtgaatc caaactgccc ctgcctctca 3181 aagcagacgg caaagaagaa ggcactccac agcccgagag caagtcaaag aagtccagct 3241 cctccaccac tactggggag aagatcacga aggtgtacga gctggggaat gagccagaga 3301 gaaagctctg ggtcgaccga tacctcacct tcatggaaga gagaggctct cctgtctcaa 3361 gtctgcctgc cgtgggcaag aagcccctgg acctgttccg actctacgtc tgcgtcaaag 3421 agatcggggg tttggcccag gttaataaaa acaagaagtg gcgtgagctg gcaaccaacc 3481 taaacgttgg cacctcaagc agtgcagcga gctccctgaa aaagcagtat attcagtacc 3541 tgtttgcctt tgagtgcaag atcgaacgtg gggaggagcc cccgccggaa gtcttcagca 3601 ccggggacac caaaaagcag cccaagctcc agccgccatc tcctgctaac tcgggatcct 3661 tgcaaggccc acagaccccc cagtcaactg gcagcaattc catggcagag gttccaggtg 3721 acctgaagcc acctacccca gcctccaccc ctcacggcca gatgactcca atgcaaggtg 3781 gaagaagcag tacaatcagt gtgcacgacc cattctcaga tgtgagtgat tcatccttcc 3841 cgaaacggaa ctccatgact ccaaacgccc cctaccagca gggcatgagc atgcccgatg 3901 tgatgggcag gatgccctat gagcccaaca aggacccctt tgggggaatg agaaaagtgc 3961 ctggaagcag cgagcccttt atgacgcaag gacagatgcc caacagcagc atgcaggaca 4021 tgtacaacca aagtccctcc ggagcaatgt ctaacctggg catggggcag cgccagcagt 4081 ttccctatgg agccagttac gaccgaaggc atgaacctta tgggcagcag tatccaggcc 4141 aaggccctcc ctcgggacag ccgccgtatg gagggcacca gcccggcctg tacccacagc 4201 agccgaatta caaacgccat atggacggca tgtacgggcc cccagccaag cgccacgagg 4261 gcgacatgta caacatgcag tacagcagcc agcagcagga gatgtacaac cagtatggag 4321 gctcctactc gggcccggac cgcaggccca tccagggcca gtacccgtat ccctacagca 4381 gggagaggat gcagggcccg gggcagatcc agacacacgg aatcccgcct cagatgatgg 4441 gcggcccgct gcagtcgtcc tccagtgagg ggcctcagca gaatatgtgg gcagcacgca 4501 atgatatgcc ttatccctac cagaacaggc agggccctgg cggccctaca caggcgcccc 4561 cttacccagg catgaaccgc acagacgata tgatggtacc cgatcagagg ataaatcatg 4621 agagccagtg gccttctcac gtcagccagc gtcagcctta tatgtcgtcc tcagcctcca 4681 tgcagcccat cacacgccca ccacagccgt cctaccagac gccaccgtca ctgccaaatc 4741 acatctccag ggcgcccagc ccagcgtcct tccagcgctc cctggagaac cgcatgtctc 4801 caagcaagtc tccttttctg ccgtctatga agatgcagaa ggtcatgccc acggtcccca 4861 catcccaggt caccgggcca ccaccccaac cacccccaat cagaagggag atcacctttc 4921 ctcctggctc agtagaagca tcacaaccag tcttgaaaca aaggcgaaag attacctcca 4981 aagatatcgt tactcctgag gcgtggcgtg tgatgatgtc ccttaaatca ggtcttttgg 5041 ctgagagtac gtgggctttg gacactatta atattcttct gtatgatgac agcactgttg 5101 ctactttcaa tctctcccag ttgtctggat ttctcgaact tttagtcgag tactttagaa 5161 aatgcctgat tgacattttt ggaattctta tggaatatga agtgggagac cccagccaaa 5221 aagcacttga tcacaacgca gcaaggaagg atgacagcca gtccttggca gacgattctg 5281 ggaaagagga ggaagatgct gaatgtattg atgacgacga ggaagacgag gaggatgagg 5341 aggaagacag cgagaagaca gaaagcgatg aaaagagcag catcgctctg actgccccgg 5401 acgccgctgc agacccaaag gagaagccca agcaagccag taagttcgac aagctgccaa 5461 taaagatagt caaaaagaac aacctgtttg ttgttgaccg atctgacaag ttggggcgtg 5521 tgcaggagtt caatagtggc cttctgcact ggcagctcgg cgggggtgac accaccgagc 5581 acattcagac tcactttgag agcaagatgg aaattcctcc tcgcaggcgc ccacctcccc 5641 ccttaagctc cgcaggtaga aagaaagagc aagaaggcaa aggcgactct gaagagcagc 5701 aagagaaaag catcatagca accatcgatg acgtcctctc tgctcggcca ggggcattgc 5761 ctgaagacgc aaaccctggg ccccagaccg aaagcagtaa gtttcccttt ggtatccagc 5821 aagccaaaag tcaccggaac atcaagctgc tggaggacga gcccaggagc cgagacgaga 5881 ctcctctgtg taccatcgcg cactggcagg actcgctggc taagcgatgc atctgtgtgt 5941 ccaatattgt ccgtagcttg tcattcgtgc ctggcaatga tgccgaaatg tccaaacatc 6001 caggcctggt gctgatcctg gggaagctga ttcttcttca ccacgagcat ccagagagaa 6061 agcgagcacc gcagacctat gagaaagagg aggatgagga caagggggtg gcctgcagca 6121 aagatgagtg gtggtgggac tgcctcgagg tcttgaggga taacacgttg gtcacgttgg 6181 ccaacatttc cgggcagcta gacttgtctg cttacacgga aagcatctgc ttgccaattt 6241 tggatggctt gctgcactgg atggtgtgcc cgtctgcaga ggcacaagat ccctttccaa 6301 ctgtgggacc caactcggtc ctgtcgcctc agagacttgt gctggagacc ctctgtaaac 6361 tcagtatcca ggacaataat gtggacctga tcttggccac tcctccattt agtcgtcagg 6421 agaaattcta tgctacatta gttaggtacg ttggggatcg caaaaaccca gtctgtcgag 6481 aaatgtccat ggcgctttta tcgaaccttg cccaagggga cgcactagca gcaagggcca 6541 tagctgtgca gaaaggaagc attggaaact tgataagctt cctagaggat ggggtcacga 6601 tggcccagta ccagcagagc cagcacaacc tcatgcacat gcagcccccg cccctggaac 6661 cacctagcgt agacatgatg tgcagggcgg ccaaggcttt gctagccatg gccagagtgg 6721 acgaaaaccg ctcggaattc cttttgcacg agggccggtt gctggatatc tcgatatcag 6781 ctgtcctgaa ctctctggtt gcatctgtca tctgtgatgt actgtttcag attgggcagt 6841 tatgacataa gtgagaaggc aagcatgtgt gagtgaagat tagagggtca catataactg 6901 gctgttttct gttcttgttt atccagcgta ggaagaagga aaagaaaatc tttgctcctc 6961 tgccccattc actatttacc aattgggaat taaagaaata attaatttga acagttatga 7021 aattaatatt tgctgtctgt gtgtataagt acatcctttg gggttttttt tttctctttt 7081 ttttaaccaa agttgctgtc tagtgcattc aaaggtcact ttttgttctt cacagatcct 7141 gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa 7201 ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc 7261 ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa 7321 ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa ctttagcttg 7381 catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa gtactttctc 7441 taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc acagttttag 7501 agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg 7561 cgtggaaata ttcttattgg tagaaacaac tacaccctgg tcatcatcct gcctttctct 7621 ttatggttac aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg 7681 ggcccctctg ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg 7741 ctggttgttg tgctgtctca tcattttggc aaagaattca ctcctcaggt gcaggctgcc 7801 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt 7861 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc 7921 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc 7981 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt 8041 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat 8101 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga 8161 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt 8221 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct 8281 gtccctcttc tcttatggag atccctcgag gagctttttg caaaagccta ggcctccaaa 8341 aaagcctctt cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata 8401 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg 8461 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgctgc attaatgaat 8521 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 8581 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 8641 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8701 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8761 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8821 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8881 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8941 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 9001 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 9061 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 9121 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 9181 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 9241 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 9301 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 9361 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 9421 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 9481 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 9541 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 9601 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 9661 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 9721 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 9781 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 9841 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 9901 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 9961 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 10021 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 10081 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 10141 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 10201 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 10261 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 10321 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 10381 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 10441 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 10501 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 10561 t //
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