| 质粒名称: | BE4 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 100802 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3402 bp |
| 完整质粒大小: | 8877 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | BE |
| 物种来源: | R、 褐家鼠，合成；化脓链球菌 |
| 插入大小: | 5475 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 吉布森克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 |
| 3' 测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | BE4 was a gift from David Liu (Addgene plasmid # 100802 ; http://n2t.net/addgene:100802 ; RRID:Addgene_100802) |
| 参考文献部分: | Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. aao4774 [pii] PubMed 28875174 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS BE4 8877 bp ds-DNA circular SYN 13-SEP-2018
DEFINITION Expresses BE4 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS BE4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8877)
AUTHORS Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW,
Kim YB, Badran AH, Liu DR
TITLE Improved base excision repair inhibition and bacteriophage Mu Gam
protein yields C:G-to-T:A base editors with higher efficiency and
product purity.
JOURNAL Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774.
eCollection 2017 Aug.
PUBMED 28875174
REFERENCE 2 (bases 1 to 8877)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 13, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8877
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 409..1095
/codon_start=1
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/label=APOBEC-1
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
LWATGLK"
CDS 1192..5292
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5323..5571
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5602..5850
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5863..5883
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5892..5909
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5932..5949)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5938..6162
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6233..6249)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6233..6249)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6246..6268)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6257..6273
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6281..6311)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6326..6347
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6464..6481)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6635..7223)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6715..6734)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7394..8254)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8017..8036
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8255..8359)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8438..8457)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8629..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gagctcagag
421 actggcccag tggctgtgga ccccacattg agacggcgga tcgagcccca tgagtttgag
481 gtattcttcg atccgagaga gctccgcaag gagacctgcc tgctttacga aattaattgg
541 gggggccggc actccatttg gcgacataca tcacagaaca ctaacaagca cgtcgaagtc
601 aacttcatcg agaagttcac gacagaaaga tatttctgtc cgaacacaag gtgcagcatt
661 acctggtttc tcagctggag cccatgcggc gaatgtagta gggccatcac tgaattcctg
721 tcaaggtatc cccacgtcac tctgtttatt tacatcgcaa ggctgtacca ccacgctgac
781 ccccgcaatc gacaaggcct gcgggatttg atctcttcag gtgtgactat ccaaattatg
841 actgagcagg agtcaggata ctgctggaga aactttgtga attatagccc gagtaatgaa
901 gcccactggc ctaggtatcc ccatctgtgg gtacgactgt acgttcttga actgtactgc
961 atcatactgg gcctgcctcc ttgtctcaac attctgagaa ggaagcagcc acagctgaca
1021 ttctttacca tcgctcttca gtcttgtcat taccagcgac tgcccccaca cattctctgg
1081 gccaccgggt tgaaatctgg tggttcttct ggtggttcta gcggcagcga gactcccggg
1141 acctcagagt ccgccacacc cgaaagttct ggtggttctt ctggtggttc tgataaaaag
1201 tattctattg gtttagccat cggcactaat tccgttggat gggctgtcat aaccgatgaa
1261 tacaaagtac cttcaaagaa atttaaggtg ttggggaaca cagaccgtca ttcgattaaa
1321 aagaatctta tcggtgccct cctattcgat agtggcgaaa cggcagaggc gactcgcctg
1381 aaacgaaccg ctcggagaag gtatacacgt cgcaagaacc gaatatgtta cttacaagaa
1441 atttttagca atgagatggc caaagttgac gattctttct ttcaccgttt ggaagagtcc
1501 ttccttgtcg aagaggacaa gaaacatgaa cggcacccca tctttggaaa catagtagat
1561 gaggtggcat atcatgaaaa gtacccaacg atttatcacc tcagaaaaaa gctagttgac
1621 tcaactgata aagcggacct gaggttaatc tacttggctc ttgcccatat gataaagttc
1681 cgtgggcact ttctcattga gggtgatcta aatccggaca actcggatgt cgacaaactg
1741 ttcatccagt tagtacaaac ctataatcag ttgtttgaag agaaccctat aaatgcaagt
1801 ggcgtggatg cgaaggctat tcttagcgcc cgcctctcta aatcccgacg gctagaaaac
1861 ctgatcgcac aattacccgg agagaagaaa aatgggttgt tcggtaacct tatagcgctc
1921 tcactaggcc tgacaccaaa ttttaagtcg aacttcgact tagctgaaga tgccaaattg
1981 cagcttagta aggacacgta cgatgacgat ctcgacaatc tactggcaca aattggagat
2041 cagtatgcgg acttattttt ggctgccaaa aaccttagcg atgcaatcct cctatctgac
2101 atactgagag ttaatactga gattaccaag gcgccgttat ccgcttcaat gatcaaaagg
2161 tacgatgaac atcaccaaga cttgacactt ctcaaggccc tagtccgtca gcaactgcct
2221 gagaaatata aggaaatatt ctttgatcag tcgaaaaacg ggtacgcagg ttatattgac
2281 ggcggagcga gtcaagagga attctacaag tttatcaaac ccatattaga gaagatggat
2341 gggacggaag agttgcttgt aaaactcaat cgcgaagatc tactgcgaaa gcagcggact
2401 ttcgacaacg gtagcattcc acatcaaatc cacttaggcg aattgcatgc tatacttaga
2461 aggcaggagg atttttatcc gttcctcaaa gacaatcgtg aaaagattga gaaaatccta
2521 acctttcgca taccttacta tgtgggaccc ctggcccgag ggaactctcg gttcgcatgg
2581 atgacaagaa agtccgaaga aacgattact ccatggaatt ttgaggaagt tgtcgataaa
2641 ggtgcgtcag ctcaatcgtt catcgagagg atgaccaact ttgacaagaa tttaccgaac
2701 gaaaaagtat tgcctaagca cagtttactt tacgagtatt tcacagtgta caatgaactc
2761 acgaaagtta agtatgtcac tgagggcatg cgtaaacccg cctttctaag cggagaacag
2821 aagaaagcaa tagtagatct gttattcaag accaaccgca aagtgacagt taagcaattg
2881 aaagaggact actttaagaa aattgaatgc ttcgattctg tcgagatctc cggggtagaa
2941 gatcgattta atgcgtcact tggtacgtat catgacctcc taaagataat taaagataag
3001 gacttcctgg ataacgaaga gaatgaagat atcttagaag atatagtgtt gactcttacc
3061 ctctttgaag atcgggaaat gattgaggaa agactaaaaa catacgctca cctgttcgac
3121 gataaggtta tgaaacagtt aaagaggcgt cgctatacgg gctggggacg attgtcgcgg
3181 aaacttatca acgggataag agacaagcaa agtggtaaaa ctattctcga ttttctaaag
3241 agcgacggct tcgccaatag gaactttatg cagctgatcc atgatgactc tttaaccttc
3301 aaagaggata tacaaaaggc acaggtttcc ggacaagggg actcattgca cgaacatatt
3361 gcgaatcttg ctggttcgcc agccatcaaa aagggcatac tccagacagt caaagtagtg
3421 gatgagctag ttaaggtcat gggacgtcac aaaccggaaa acattgtaat cgagatggca
3481 cgcgaaaatc aaacgactca gaaggggcaa aaaaacagtc gagagcggat gaagagaata
3541 gaagagggta ttaaagaact gggcagccag atcttaaagg agcatcctgt ggaaaatacc
3601 caattgcaga acgagaaact ttacctctat tacctacaaa atggaaggga catgtatgtt
3661 gatcaggaac tggacataaa ccgtttatct gattacgacg tcgatcacat tgtaccccaa
3721 tcctttttga aggacgattc aatcgacaat aaagtgctta cacgctcgga taagaaccga
3781 gggaaaagtg acaatgttcc aagcgaggaa gtcgtaaaga aaatgaagaa ctattggcgg
3841 cagctcctaa atgcgaaact gataacgcaa agaaagttcg ataacttaac taaagctgag
3901 aggggtggct tgtctgaact tgacaaggcc ggatttatta aacgtcagct cgtggaaacc
3961 cgccaaatca caaagcatgt tgcacagata ctagattccc gaatgaatac gaaatacgac
4021 gagaacgata agctgattcg ggaagtcaaa gtaatcactt taaagtcaaa attggtgtcg
4081 gacttcagaa aggattttca attctataaa gttagggaga taaataacta ccaccatgcg
4141 cacgacgctt atcttaatgc cgtcgtaggg accgcactca ttaagaaata cccgaagcta
4201 gaaagtgagt ttgtgtatgg tgattacaaa gtttatgacg tccgtaagat gatcgcgaaa
4261 agcgaacagg agataggcaa ggctacagcc aaatacttct tttattctaa cattatgaat
4321 ttctttaaga cggaaatcac tctggcaaac ggagagatac gcaaacgacc tttaattgaa
4381 accaatgggg agacaggtga aatcgtatgg gataagggcc gggacttcgc gacggtgaga
4441 aaagttttgt ccatgcccca agtcaacata gtaaagaaaa ctgaggtgca gaccggaggg
4501 ttttcaaagg aatcgattct tccaaaaagg aatagtgata agctcatcgc tcgtaaaaag
4561 gactgggacc cgaaaaagta cggtggcttc gatagcccta cagttgccta ttctgtccta
4621 gtagtggcaa aagttgagaa gggaaaatcc aagaaactga agtcagtcaa agaattattg
4681 gggataacga ttatggagcg ctcgtctttt gaaaagaacc ccatcgactt ccttgaggcg
4741 aaaggttaca aggaagtaaa aaaggatctc ataattaaac taccaaagta tagtctgttt
4801 gagttagaaa atggccgaaa acggatgttg gctagcgccg gagagcttca aaaggggaac
4861 gaactcgcac taccgtctaa atacgtgaat ttcctgtatt tagcgtccca ttacgagaag
4921 ttgaaaggtt cacctgaaga taacgaacag aagcaacttt ttgttgagca gcacaaacat
4981 tatctcgacg aaatcataga gcaaatttcg gaattcagta agagagtcat cctagctgat
5041 gccaatctgg acaaagtatt aagcgcatac aacaagcaca gggataaacc catacgtgag
5101 caggcggaaa atattatcca tttgtttact cttaccaacc tcggcgctcc agccgcattc
5161 aagtattttg acacaacgat agatcgcaaa cgatacactt ctaccaagga ggtgctagac
5221 gcgacactga ttcaccaatc catcacggga ttatatgaaa ctcggataga tttgtcacag
5281 cttgggggtg actctggtgg ttctggagga tctggtggtt ctactaatct gtcagatatt
5341 attgaaaagg agaccggtaa gcaactggtt atccaggaat ccatcctcat gctcccagag
5401 gaggtggaag aagtcattgg gaacaagccg gaaagcgata tactcgtgca caccgcctac
5461 gacgagagca ccgacgagaa tgtcatgctt ctgactagcg acgcccctga atacaagcct
5521 tgggctctgg tcatacagga tagcaacggt gagaacaaga ttaagatgct ctctggtggt
5581 tctggaggat ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag
5641 caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg
5701 aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat
5761 gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat
5821 agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa
5881 gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
5941 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
6001 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
6061 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
6121 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
6181 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
6241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
6301 taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
6361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
6421 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
6481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
6541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
6601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
6661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
6721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
6781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
6841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
6901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
6961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
7021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
7081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
7141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
7201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
7261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
7321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
7381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
7441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
7501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
7561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
7621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
7681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
7741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
7801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
7861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
7921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
7981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
8041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
8101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
8161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
8221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
8281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
8341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
8401 gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat
8461 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
8521 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
8581 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
8641 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
8701 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
8761 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
8821 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc
//
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