CaV1.2

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相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: CaV1.2,pCDNA6-V5-His-CaV1.2-m
出品公司: 爱迪基因
目录编号: 26572
存储实验室: Diane Lipscombe

载体骨架基本信息
载体名称: pcDNA6/V5-His
载体出品公司: Invitrogen
载体抗性: 氨苄青霉素
空载体大小: 5100 bp
完整质粒大小: --
载体修饰: --
载体类型: 哺乳细胞表达
筛选标记: --
高拷贝/低拷贝: --
生长条件: 37 ℃

插入基因信息
插入基因名称: Calcium channel, voltage-dependent, L type, alpha 1C subunit
物种来源: M. musculus (mouse)
插入大小: 6600 bp
基因突变: --
GenBank编号 --
启动子 --
标签/融合蛋白: --
克隆方法: 限制性内切酶
5' 克隆位点: KpnI(销毁后未知)
3' 克隆位点: NotI(销毁后未知)
5' 测序引物 T7
3' 测序引物 BGH-rev

文献引用方法
材料和方法部分: CaV1.2 was a gift from Diane Lipscombe (Addgene plasmid # 26572 ; http://n2t.net/addgene:26572 ; RRID:Addgene_26572)
参考文献部分: Neuronal L-type calcium channels open quickly and are inhibited slowly. Helton TD, Xu W, Lipscombe D. J Neurosci. 2005 Nov 2. 25(44):10247-51. 10.1523/JNEUROSCI.1089-05.2005 PubMed 16267232

载体图谱质粒图谱和多克隆位点信息

CaV1.2质粒图谱

载体简介

载体序列

LOCUS       CaV1.2               11709 bp ds-DNA     circular SYN 09-APR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    CaV1.2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Helton TD, Xu W, Lipscombe D
  TITLE     Neuronal L-type calcium channels open quickly and are inhibited 
            slowly.
  JOURNAL   J Neurosci. 2005 Nov 2. 25(44):10247-51.
  PUBMED    16267232
REFERENCE   2  (bases 1 to 11709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 9, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    270..391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(281..300)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     335..354
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(549..947)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(966..1013)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1061..1257)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(1069..1279)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1075..1210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1129..1148)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1303..1323
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1332..1760)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1442..1463)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1654..1673
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1806..2030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2019..2036
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2059..2076)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2086..2127)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     RBS             6340..6351
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(8891..8910)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8892..8910)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8955..9158)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8984..9004)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9159..9538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9710..9729
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9804..9908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9909..10769
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10127..10146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10940..11528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11429..11448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11682..11699
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
      241 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac
      301 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      361 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat
      421 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
      481 catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca
      541 cgaagtgctt agccctccca cacataacca gagggcagca attcacgaat cccaactgcc
      601 gtcggctgtc catcactgtc cttcactatg gctttgatcc caggatgcag atcgagaagc
      661 acctgtcggc accgtccgca ggggctcaag atgcccctgt tctcatttcc gatcgcgacg
      721 atacaagtca ggttgccagc tgccgcagca gcagcagtgc ccagcaccac gagttctgca
      781 caaggtcccc cagtaaaatg atatacattg acaccagtga agatgcggcc gtcgctagag
      841 agagctgcgc tggcgacgct gtagtcttca gagatgggga tgctgttgat tgtagccgtt
      901 gctctttcaa tgagggtgga ttcttcttga gacaaaggct tggccatggt ttagttcctc
      961 accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct
     1021 gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc
     1081 caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg
     1141 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     1201 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc
     1261 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     1321 tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     1381 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     1441 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     1501 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     1561 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     1621 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     1681 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     1741 tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc
     1801 agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc
     1861 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga
     1921 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga
     1981 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga
     2041 tcagcgggtt taaactcaat ggtgatggtg atgatgaccg gtacgcgtag aatcgagacc
     2101 gaggagaggg ttagggatag gcttaccttc gaagggccct ctagactcga gcggccgcca
     2161 gtgtgatgga tatctgcaga attcggcttg cttatcccga cagcagtgaa tgaagacgct
     2221 gttccggtta cctccaggtc acgagaacag tgaggcactt ctgaaacgaa cccattagga
     2281 acattgaaac gaataaaaaa accacccgcg tctcgccagc cctgaggact acaggttgct
     2341 gacgtaggac ctgctgtccg cgagcgcctc ctcgctccgg cctcgcccca gggcatatgc
     2401 gcagctctcg tcctctggga ccacagccct gtcctgcccc gggtccctgc agttcacaaa
     2461 aggtaagagg gtgccgttgg ggctctgctg ggcgccccca ctgaggatgt tgtctgcggc
     2521 gttctccatc tcctctattg tcatgtcgca ggcgtcagcc agctcctggg tggtgacctc
     2581 gataaacttg ggatcttgag caaactgtcc cagtccttct gaaatcaaga ccgcttccac
     2641 caggctgctg gcactgccat ggaactgtct ccctggagct ccagcctggc tgggcacggt
     2701 gagggagacg ggccgggctc tccgggccat gctgctgctc ccactacagg ctgtcgtctc
     2761 ctcagaccag gagctgcagt ggatggatgg gaagctgctg ttgagtttct cgctggactc
     2821 tgccccctcc agccgtaggg tagggatggg ccgtaggggt ctgccccggc tgcctggagt
     2881 gacagggggt gtggggcatg gcctggggaa tgtggtagga gaatggcttc tctgcaggag
     2941 ggggctcaag cctgccactg ccaatgcctg atgatgaacc agatgcaagg gcaaggctgt
     3001 cttctgagag atgtctcccc cttgatcctt ttgtcgcttt agacattcca gatggaagga
     3061 ggcccttcga cctagagagg cagagcgaag gaaactcctc tttggagatg gctggatctc
     3121 cctcttgtcc tcctctgggc aggtcagttg tcggtgttca tcttcctggt aggagaacat
     3181 atctgtggag agcaggctgg gctcactgca gtactcggct tcttcactca tccttacgct
     3241 gcgggtctca tctggaaata tctcctgatt caccgtggct ccctggctct ctcgggagtg
     3301 gcacctctta gagctgagct tccatgctgc ctcctgtact cggacagcag gggacaaggg
     3361 aggtccatgg ccctccacag tgctgaccgt gctggagtag ccagcgggat gggggaagcg
     3421 gcccagggca gtgttgttgg cattgttgat gttggcattg gagcccgtgg atgagtagct
     3481 gctgggggtg aacgtggagt ccaccagctt ctcatgggac ggtgactcag tgtcagcttg
     3541 gttgttccct gtcttgttga tgtgcagtgg gcgctgagtg gcgaacgtct gaggaaagtt
     3601 gcccctgctg tcactctgat agtaggtgac atggttgccg aacaggcctc cagccctcct
     3661 gaagatgtca tcttcggagg cagcagacac cgcctccttc atagccttgt ccaactcctc
     3721 ctcagcagtc agatccccag agatggcccg ccggatctca ggcccaatat catgcaaggt
     3781 gcgcaagcca gcctggaggg acagtgcatt cctttgtgag ggcttgccca ccagcccctg
     3841 ctcttttcgc ttcttgaatt tcctgaagta ctcttgtatc aggaaggtgg catagaactt
     3901 gcccactgtg acctcgtcat cgcctgcagg gggcaccacc tggtccaaca gcttcatgct
     3961 ggtcctcttc cagattttct tgatgatggc ccgcagctcc tcattggctt gctctaggtt
     4021 cccttctgtt ttgatcctca gggctgtcct gacgagggca aacagggtag cattgaacat
     4081 gactgtgcca tcgctgttca gaggcatgtt catggacacc aggcgtttgc aggccacacg
     4141 gtgaggacac aatttcccaa aacccagtgg gggctgaatt cgacggagga gggtcaccac
     4201 atccaagtgt ttgatccgac ccttggcttc agggtcatac tcggcccaga ttctcttgaa
     4261 ttcatccagg tgatggggac cgaggataga ccaatcccta gtcaggtagt caaagttgtc
     4321 catgataaca gctacaaaga ggttgatgat caggaaggca cagagcatgt agaagctgat
     4381 gaagtagaag acagcaaagc tgctgccaca gggggtctcc ccttccgtgc tgttgctggg
     4441 ctcagactct ggggcacact tcttgcctgg catgcaggcc agcatgatgt cctgccaggc
     4501 ctctccggtg gcacacctga acagcagtaa cacagcctgg gggaacgtct ggaagttgtt
     4561 gttccgattg atctctgtgg tgtcattcag ggcaatcttc ccaaacacct gcatcccaat
     4621 cactgcatag ataaagaaca gcatcacaat caaaagagcc acatagggca gagcctggaa
     4681 ggacttgatg aaggtccaca gcagggttcg gatgccttcc ccgcggctca gcagcttcac
     4741 caggcgcatg acccggaaga ggcggaagaa ggtgatggag atgcgggagt tctcctctgc
     4801 actcatagag ggagagcatt gggtatgttc agctgggtgt acctcggtga ttgctatatc
     4861 aacaatgcta cccacaacaa tcaaggcgtc aaatgtattc catgcatcac agaaatagtg
     4921 cttgggtttg aaggcaatga gcttcaggat catctccact gtgaagaggc cggtgaaaag
     4981 catgttgagt atattcatgg cgattttgaa gaggcagctc tggccatagt gctgcatggc
     5041 caggcagatg gtgttgagca ggatgagaac gaacatcaga tactcgaagt aggtagagtt
     5101 gaccacgtac cacactttgt actggtgctg gttcttgggg atgtaccttc gcaagggtcg
     5161 ggccttgagg gcatattcca cacattgtct ctggttcttg tccagctcac agttcttgta
     5221 ctcttgttcc ccctgctcct ggaaggtgac aatgacgaaa cccacgaaga tgttcatcat
     5281 gaagaaggca atgatgatga tatagatgat gaagaagatg gagatctcca cacggtagtt
     5341 gtagatgggg cccttgtctt ctgtgtggga gtcaatggag cggtacagca gctctggcca
     5401 cccttcgaag gtggagacgg tgaagagagc catcatggct gccaaaacat tgtcaaagtc
     5461 aaacttgctg ttctcccagc ttcgaggctg gataatgggg tgatcgacct ctccatcttt
     5521 gtatgtgata tagttaccct tgcattctgc ctccgtctgt ttagaactat ccgaacaggt
     5581 atagagcttt cccttgaaga gctggacccc aatgcaggcg aacatgaact gcagcagagt
     5641 ggtgacaatt acgatgttcc cgatggtccg gatggccaca aacacgcact gaaccacatg
     5701 ctttagcccc ttggccctgt tgatggccct caggggtctg aggactcgca gcactcgtaa
     5761 aatcttcaca acgttgatcg cgctggactg gatgccaaag gagatgaggg acacgctaac
     5821 caccagcagg tccaggatat tgaagtagtt tcggcagaaa gagcccttgt gcaggaaagc
     5881 cccgtaagca gtcatcttaa ggataatttc taatgtaaag atactagtga agacatagtc
     5941 tgcattgcct aggatatggt tcctgaagga ggtgtgctgg acggggtcct cagcagccag
     6001 agagatgctg ctgagcagaa tgaagaagag gatgaggttg gtgaagatcg tgtcattgac
     6061 aatacggtgg cactgcaggc ggaacctgtt gtttgggctg aagatgaaaa atgcactggc
     6121 ttccggcatg ggaactgcct tttccttaag gtgcagctca gacaggggcc gggggcgtgg
     6181 ccccacaggc atctctggct cctcttcatc ctcttcccct gcagtgtctg ggttggagtg
     6241 gggactctta tcctcgtttt cactgggctg gaggtcatcc atgttgatct tggtagtggg
     6301 tggggattct ccatcggctg taatggattt cagttcaatt ttctcctctt tgctctcctc
     6361 cacggctggc ttctccatca cctcctgttt cttttctggg ctggcagtcc tggccagctt
     6421 cttcctctcc ttctcttctt cctcctcctt ttgggctgag gtcaggctct ccgcatcagc
     6481 caggttgtcc accgcaatgg ccaagaacac attcagtagg atataatttc cacagatgaa
     6541 gaggatgatg aagtaaatac agactaacat ccctggaaaa gaggggccgc cataagccat
     6601 gatcccatca tacatcaccg aattccagtc ctccccggtc aggatctgaa acacagtgag
     6661 gagagactgc gggaagttat cgaacgtgct cctacgggtc tgcatctcat cgaaattgaa
     6721 cttccctcca aagagctgca tccccaggag ggagaagatg atgatgaaga ggaagaggag
     6781 cagcagcagg gaggcaatgg agcgcactga gttcagcaag gatgccacaa ggttgctcaa
     6841 ggaattccag tacctggtga tcttgaagat cctgagcaac cgcacacatc tcagcacaga
     6901 gatgcccagg ggagacatga tcttcgtctc caccaggatg gtctccagga tgcccccaca
     6961 cacaatgaaa cagtcaaagc ggttgaagag ggacacaaaa taggcctgaa gacccaggct
     7021 gtacatcttc aggagcattt ctgcagtgaa aagggccagg agggctttat tggctgtgtc
     7081 ttgcacttct gtgagccagt gaggctggtt gtaatgttcg gaggcaatgg tgagggtgtt
     7141 gaggaacacc aggaagatca cgagccagta gaagacgttg gacttaactg ctgcacggca
     7201 ttttcttctg cagaatcgat tccacctgcg ccagtagcgg ctgaatttgg atttggagat
     7261 ccgatgggca agcctggctc cacagttttc tccctcgatg tcacctccag ccacgttttc
     7321 agtgttgaca gactcagtct cacttgtggg catgctcatg tttcgaggct tgtcttcatc
     7381 catgccctcg tcctcattct cggggtcaat gtcttctgcc tgggtgatcc agtccaggta
     7441 gcctttgaga tcttcttcta gttgctgctt ctctcgaagc ttctggaaat ctcctcgggc
     7501 tttggctttc tccctctctt tggaaaactc cccgctcaaa acaccgagaa ccagatttag
     7561 aacgaaaaag gatccaaaga tgaccagact gacaaaatac acccagggca actcatagcc
     7621 catagcgtct tgcatccagt acagcacgtc tgtccagccc tccatggtga tacactggaa
     7681 caccgtcagc atggcgaagg cgaagttgtc gaagttggtg atgccgtgct tgggcccatc
     7741 ccacccgggt ttgcacacgg tcccgttctg acactgtcgc ccatggcctg tctccaaagc
     7801 acaaggggaa ggatcctctt ctgccggaac atctattatg ccctcctggt tgtagcaggt
     7861 cttgtgcatc tttcccatga agagctccag gccgataata gcataaatga tgatgacgaa
     7921 gagcacaaga agggcaatgt gcagcagagg caccatggcc ttgatgatgg agttcaggac
     7981 cacctggaga cttgggactc cggacactag ccgcagtgga cggagcacgc ggaacgctct
     8041 cagtgccttc acgtcgaatc cagctccttt ccctcctaga gcattggccc catcagcttt
     8101 ggttgcttgt tctaaaattg cactaaaaag ccctacaacc acgattataa aatccagtaa
     8161 attccaacca ttgcggaggt aagcgttggg gtggaagaga agtccgtagg caattacttt
     8221 taaaaatgct tccacggtaa aaatgatgag gaagagatac tccactcgtt ccaggttgga
     8281 gttggtggcg ttggagtcgt cttccggaaa gggaatatag attgctaagg ccacacaatt
     8341 ggcaaaaata gtcagtaaaa taatgatttc aaatggtttc cattcaacaa tgcttatgca
     8401 cgccctccgg atagggttct tcagggtcag acacagcaga gcccggggcg gccgtgtggc
     8461 ggttgtgccc ccctgcttct tgggtttccc atactgctgc cgcttccgct gtgtggaact
     8521 gacggtagag atggttgcgt tgccagcact gcccatgagc ttggcctgcc gggcggcatc
     8581 gatggctgcc tgccaggaca gtgctgcccc tggagtaggg atgtgctcgg gagcaagtcc
     8641 tgcagctgca ttggcattca tgttggcatg agctgggcgt gggctcccat agttggaacc
     8701 ttggtggttt tcctctggaa cgtacatcct cgtgttttca ttgaccattg gccaaaaact
     8761 gcttggaaag gagaagccac aaagaggaag aaatgcagca acaagccgaa ttccagcaca
     8821 ctggcggccg ttactagtgg atccgagctc ggtaccaagc ttaagtttaa acgctagcca
     8881 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
     8941 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     9001 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     9061 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     9121 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     9181 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     9241 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     9301 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     9361 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     9421 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     9481 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
     9541 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
     9601 ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac
     9661 tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag
     9721 cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag
     9781 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10321 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11701 aagcggaag
//

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