质粒名称: | CaV1.2,pCDNA6-V5-His-CaV1.2-m |
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出品公司: | 爱迪基因 |
目录编号: | 26572 |
存储实验室: | Diane Lipscombe |
载体骨架基本信息 |
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载体名称: | pcDNA6/V5-His |
载体出品公司: | Invitrogen |
载体抗性: | 氨苄青霉素 |
空载体大小: | 5100 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
高拷贝/低拷贝: | -- |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Calcium channel, voltage-dependent, L type, alpha 1C subunit |
物种来源: | M. musculus (mouse) |
插入大小: | 6600 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | KpnI(销毁后未知) |
3' 克隆位点: | NotI(销毁后未知) |
5' 测序引物 | T7 |
3' 测序引物 | BGH-rev |
文献引用方法 |
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材料和方法部分: | CaV1.2 was a gift from Diane Lipscombe (Addgene plasmid # 26572 ; http://n2t.net/addgene:26572 ; RRID:Addgene_26572) |
参考文献部分: | Neuronal L-type calcium channels open quickly and are inhibited slowly. Helton TD, Xu W, Lipscombe D. J Neurosci. 2005 Nov 2. 25(44):10247-51. 10.1523/JNEUROSCI.1089-05.2005 PubMed 16267232 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS CaV1.2 11709 bp ds-DNA circular SYN 09-APR-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS CaV1.2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11709) AUTHORS Helton TD, Xu W, Lipscombe D TITLE Neuronal L-type calcium channels open quickly and are inhibited slowly. JOURNAL J Neurosci. 2005 Nov 2. 25(44):10247-51. PUBMED 16267232 REFERENCE 2 (bases 1 to 11709) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11709 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 186..208 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 205..221 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" polyA_signal 270..391 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(281..300) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 335..354 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(549..947) /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" promoter complement(966..1013) /label=EM7 promoter /note="synthetic bacterial promoter " promoter complement(1061..1257) /label=SV40 promoter /note="SV40 early promoter" promoter complement(1069..1279) /label=SV40 promoter /note="SV40 early promoter" rep_origin 1075..1210 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(1129..1148) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 1303..1323 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin complement(1332..1760) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1442..1463) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1654..1673 /label=F1ori-R /note="F1 origin, reverse primer" polyA_signal 1806..2030 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind 2019..2036 /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" CDS complement(2059..2076) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS complement(2086..2127) /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" RBS 6340..6351 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" primer_bind complement(8891..8910) /label=T7 /note="T7 promoter, forward primer" promoter complement(8892..8910) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(8955..9158) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(8984..9004) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 9159..9538 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind 9710..9729 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 9804..9908 /gene="bla" /label=AmpR promoter CDS 9909..10769 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10127..10146) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 10940..11528 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11429..11448 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11682..11699 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct 241 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac 301 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 361 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat 421 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt 481 catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca 541 cgaagtgctt agccctccca cacataacca gagggcagca attcacgaat cccaactgcc 601 gtcggctgtc catcactgtc cttcactatg gctttgatcc caggatgcag atcgagaagc 661 acctgtcggc accgtccgca ggggctcaag atgcccctgt tctcatttcc gatcgcgacg 721 atacaagtca ggttgccagc tgccgcagca gcagcagtgc ccagcaccac gagttctgca 781 caaggtcccc cagtaaaatg atatacattg acaccagtga agatgcggcc gtcgctagag 841 agagctgcgc tggcgacgct gtagtcttca gagatgggga tgctgttgat tgtagccgtt 901 gctctttcaa tgagggtgga ttcttcttga gacaaaggct tggccatggt ttagttcctc 961 accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct 1021 gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc 1081 caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg 1141 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta 1201 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc 1261 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat 1321 tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 1381 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 1441 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 1501 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 1561 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 1621 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 1681 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 1741 tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc tttccgcctc 1801 agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc 1861 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga 1921 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga 1981 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggctga 2041 tcagcgggtt taaactcaat ggtgatggtg atgatgaccg gtacgcgtag aatcgagacc 2101 gaggagaggg ttagggatag gcttaccttc gaagggccct ctagactcga gcggccgcca 2161 gtgtgatgga tatctgcaga attcggcttg cttatcccga cagcagtgaa tgaagacgct 2221 gttccggtta cctccaggtc acgagaacag tgaggcactt ctgaaacgaa cccattagga 2281 acattgaaac gaataaaaaa accacccgcg tctcgccagc cctgaggact acaggttgct 2341 gacgtaggac ctgctgtccg cgagcgcctc ctcgctccgg cctcgcccca gggcatatgc 2401 gcagctctcg tcctctggga ccacagccct gtcctgcccc gggtccctgc agttcacaaa 2461 aggtaagagg gtgccgttgg ggctctgctg ggcgccccca ctgaggatgt tgtctgcggc 2521 gttctccatc tcctctattg tcatgtcgca ggcgtcagcc agctcctggg tggtgacctc 2581 gataaacttg ggatcttgag caaactgtcc cagtccttct gaaatcaaga ccgcttccac 2641 caggctgctg gcactgccat ggaactgtct ccctggagct ccagcctggc tgggcacggt 2701 gagggagacg ggccgggctc tccgggccat gctgctgctc ccactacagg ctgtcgtctc 2761 ctcagaccag gagctgcagt ggatggatgg gaagctgctg ttgagtttct cgctggactc 2821 tgccccctcc agccgtaggg tagggatggg ccgtaggggt ctgccccggc tgcctggagt 2881 gacagggggt gtggggcatg gcctggggaa tgtggtagga gaatggcttc tctgcaggag 2941 ggggctcaag cctgccactg ccaatgcctg atgatgaacc agatgcaagg gcaaggctgt 3001 cttctgagag atgtctcccc cttgatcctt ttgtcgcttt agacattcca gatggaagga 3061 ggcccttcga cctagagagg cagagcgaag gaaactcctc tttggagatg gctggatctc 3121 cctcttgtcc tcctctgggc aggtcagttg tcggtgttca tcttcctggt aggagaacat 3181 atctgtggag agcaggctgg gctcactgca gtactcggct tcttcactca tccttacgct 3241 gcgggtctca tctggaaata tctcctgatt caccgtggct ccctggctct ctcgggagtg 3301 gcacctctta gagctgagct tccatgctgc ctcctgtact cggacagcag gggacaaggg 3361 aggtccatgg ccctccacag tgctgaccgt gctggagtag ccagcgggat gggggaagcg 3421 gcccagggca gtgttgttgg cattgttgat gttggcattg gagcccgtgg atgagtagct 3481 gctgggggtg aacgtggagt ccaccagctt ctcatgggac ggtgactcag tgtcagcttg 3541 gttgttccct gtcttgttga tgtgcagtgg gcgctgagtg gcgaacgtct gaggaaagtt 3601 gcccctgctg tcactctgat agtaggtgac atggttgccg aacaggcctc cagccctcct 3661 gaagatgtca tcttcggagg cagcagacac cgcctccttc atagccttgt ccaactcctc 3721 ctcagcagtc agatccccag agatggcccg ccggatctca ggcccaatat catgcaaggt 3781 gcgcaagcca gcctggaggg acagtgcatt cctttgtgag ggcttgccca ccagcccctg 3841 ctcttttcgc ttcttgaatt tcctgaagta ctcttgtatc aggaaggtgg catagaactt 3901 gcccactgtg acctcgtcat cgcctgcagg gggcaccacc tggtccaaca gcttcatgct 3961 ggtcctcttc cagattttct tgatgatggc ccgcagctcc tcattggctt gctctaggtt 4021 cccttctgtt ttgatcctca gggctgtcct gacgagggca aacagggtag cattgaacat 4081 gactgtgcca tcgctgttca gaggcatgtt catggacacc aggcgtttgc aggccacacg 4141 gtgaggacac aatttcccaa aacccagtgg gggctgaatt cgacggagga gggtcaccac 4201 atccaagtgt ttgatccgac ccttggcttc agggtcatac tcggcccaga ttctcttgaa 4261 ttcatccagg tgatggggac cgaggataga ccaatcccta gtcaggtagt caaagttgtc 4321 catgataaca gctacaaaga ggttgatgat caggaaggca cagagcatgt agaagctgat 4381 gaagtagaag acagcaaagc tgctgccaca gggggtctcc ccttccgtgc tgttgctggg 4441 ctcagactct ggggcacact tcttgcctgg catgcaggcc agcatgatgt cctgccaggc 4501 ctctccggtg gcacacctga acagcagtaa cacagcctgg gggaacgtct ggaagttgtt 4561 gttccgattg atctctgtgg tgtcattcag ggcaatcttc ccaaacacct gcatcccaat 4621 cactgcatag ataaagaaca gcatcacaat caaaagagcc acatagggca gagcctggaa 4681 ggacttgatg aaggtccaca gcagggttcg gatgccttcc ccgcggctca gcagcttcac 4741 caggcgcatg acccggaaga ggcggaagaa ggtgatggag atgcgggagt tctcctctgc 4801 actcatagag ggagagcatt gggtatgttc agctgggtgt acctcggtga ttgctatatc 4861 aacaatgcta cccacaacaa tcaaggcgtc aaatgtattc catgcatcac agaaatagtg 4921 cttgggtttg aaggcaatga gcttcaggat catctccact gtgaagaggc cggtgaaaag 4981 catgttgagt atattcatgg cgattttgaa gaggcagctc tggccatagt gctgcatggc 5041 caggcagatg gtgttgagca ggatgagaac gaacatcaga tactcgaagt aggtagagtt 5101 gaccacgtac cacactttgt actggtgctg gttcttgggg atgtaccttc gcaagggtcg 5161 ggccttgagg gcatattcca cacattgtct ctggttcttg tccagctcac agttcttgta 5221 ctcttgttcc ccctgctcct ggaaggtgac aatgacgaaa cccacgaaga tgttcatcat 5281 gaagaaggca atgatgatga tatagatgat gaagaagatg gagatctcca cacggtagtt 5341 gtagatgggg cccttgtctt ctgtgtggga gtcaatggag cggtacagca gctctggcca 5401 cccttcgaag gtggagacgg tgaagagagc catcatggct gccaaaacat tgtcaaagtc 5461 aaacttgctg ttctcccagc ttcgaggctg gataatgggg tgatcgacct ctccatcttt 5521 gtatgtgata tagttaccct tgcattctgc ctccgtctgt ttagaactat ccgaacaggt 5581 atagagcttt cccttgaaga gctggacccc aatgcaggcg aacatgaact gcagcagagt 5641 ggtgacaatt acgatgttcc cgatggtccg gatggccaca aacacgcact gaaccacatg 5701 ctttagcccc ttggccctgt tgatggccct caggggtctg aggactcgca gcactcgtaa 5761 aatcttcaca acgttgatcg cgctggactg gatgccaaag gagatgaggg acacgctaac 5821 caccagcagg tccaggatat tgaagtagtt tcggcagaaa gagcccttgt gcaggaaagc 5881 cccgtaagca gtcatcttaa ggataatttc taatgtaaag atactagtga agacatagtc 5941 tgcattgcct aggatatggt tcctgaagga ggtgtgctgg acggggtcct cagcagccag 6001 agagatgctg ctgagcagaa tgaagaagag gatgaggttg gtgaagatcg tgtcattgac 6061 aatacggtgg cactgcaggc ggaacctgtt gtttgggctg aagatgaaaa atgcactggc 6121 ttccggcatg ggaactgcct tttccttaag gtgcagctca gacaggggcc gggggcgtgg 6181 ccccacaggc atctctggct cctcttcatc ctcttcccct gcagtgtctg ggttggagtg 6241 gggactctta tcctcgtttt cactgggctg gaggtcatcc atgttgatct tggtagtggg 6301 tggggattct ccatcggctg taatggattt cagttcaatt ttctcctctt tgctctcctc 6361 cacggctggc ttctccatca cctcctgttt cttttctggg ctggcagtcc tggccagctt 6421 cttcctctcc ttctcttctt cctcctcctt ttgggctgag gtcaggctct ccgcatcagc 6481 caggttgtcc accgcaatgg ccaagaacac attcagtagg atataatttc cacagatgaa 6541 gaggatgatg aagtaaatac agactaacat ccctggaaaa gaggggccgc cataagccat 6601 gatcccatca tacatcaccg aattccagtc ctccccggtc aggatctgaa acacagtgag 6661 gagagactgc gggaagttat cgaacgtgct cctacgggtc tgcatctcat cgaaattgaa 6721 cttccctcca aagagctgca tccccaggag ggagaagatg atgatgaaga ggaagaggag 6781 cagcagcagg gaggcaatgg agcgcactga gttcagcaag gatgccacaa ggttgctcaa 6841 ggaattccag tacctggtga tcttgaagat cctgagcaac cgcacacatc tcagcacaga 6901 gatgcccagg ggagacatga tcttcgtctc caccaggatg gtctccagga tgcccccaca 6961 cacaatgaaa cagtcaaagc ggttgaagag ggacacaaaa taggcctgaa gacccaggct 7021 gtacatcttc aggagcattt ctgcagtgaa aagggccagg agggctttat tggctgtgtc 7081 ttgcacttct gtgagccagt gaggctggtt gtaatgttcg gaggcaatgg tgagggtgtt 7141 gaggaacacc aggaagatca cgagccagta gaagacgttg gacttaactg ctgcacggca 7201 ttttcttctg cagaatcgat tccacctgcg ccagtagcgg ctgaatttgg atttggagat 7261 ccgatgggca agcctggctc cacagttttc tccctcgatg tcacctccag ccacgttttc 7321 agtgttgaca gactcagtct cacttgtggg catgctcatg tttcgaggct tgtcttcatc 7381 catgccctcg tcctcattct cggggtcaat gtcttctgcc tgggtgatcc agtccaggta 7441 gcctttgaga tcttcttcta gttgctgctt ctctcgaagc ttctggaaat ctcctcgggc 7501 tttggctttc tccctctctt tggaaaactc cccgctcaaa acaccgagaa ccagatttag 7561 aacgaaaaag gatccaaaga tgaccagact gacaaaatac acccagggca actcatagcc 7621 catagcgtct tgcatccagt acagcacgtc tgtccagccc tccatggtga tacactggaa 7681 caccgtcagc atggcgaagg cgaagttgtc gaagttggtg atgccgtgct tgggcccatc 7741 ccacccgggt ttgcacacgg tcccgttctg acactgtcgc ccatggcctg tctccaaagc 7801 acaaggggaa ggatcctctt ctgccggaac atctattatg ccctcctggt tgtagcaggt 7861 cttgtgcatc tttcccatga agagctccag gccgataata gcataaatga tgatgacgaa 7921 gagcacaaga agggcaatgt gcagcagagg caccatggcc ttgatgatgg agttcaggac 7981 cacctggaga cttgggactc cggacactag ccgcagtgga cggagcacgc ggaacgctct 8041 cagtgccttc acgtcgaatc cagctccttt ccctcctaga gcattggccc catcagcttt 8101 ggttgcttgt tctaaaattg cactaaaaag ccctacaacc acgattataa aatccagtaa 8161 attccaacca ttgcggaggt aagcgttggg gtggaagaga agtccgtagg caattacttt 8221 taaaaatgct tccacggtaa aaatgatgag gaagagatac tccactcgtt ccaggttgga 8281 gttggtggcg ttggagtcgt cttccggaaa gggaatatag attgctaagg ccacacaatt 8341 ggcaaaaata gtcagtaaaa taatgatttc aaatggtttc cattcaacaa tgcttatgca 8401 cgccctccgg atagggttct tcagggtcag acacagcaga gcccggggcg gccgtgtggc 8461 ggttgtgccc ccctgcttct tgggtttccc atactgctgc cgcttccgct gtgtggaact 8521 gacggtagag atggttgcgt tgccagcact gcccatgagc ttggcctgcc gggcggcatc 8581 gatggctgcc tgccaggaca gtgctgcccc tggagtaggg atgtgctcgg gagcaagtcc 8641 tgcagctgca ttggcattca tgttggcatg agctgggcgt gggctcccat agttggaacc 8701 ttggtggttt tcctctggaa cgtacatcct cgtgttttca ttgaccattg gccaaaaact 8761 gcttggaaag gagaagccac aaagaggaag aaatgcagca acaagccgaa ttccagcaca 8821 ctggcggccg ttactagtgg atccgagctc ggtaccaagc ttaagtttaa acgctagcca 8881 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct 8941 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt 9001 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa 9061 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc 9121 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat 9181 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag 9241 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga 9301 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc 9361 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg 9421 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg 9481 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa 9541 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc 9601 ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac 9661 tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag 9721 cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag 9781 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 9841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 9901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 9961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 10021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 10081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 10141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 10201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 10261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 10321 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 10381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 10441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 10501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 10561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 10621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 10681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 10741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 10801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 10861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 10921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 10981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 11041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 11101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 11161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 11221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 11281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 11341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 11401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 11461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 11521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 11581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 11641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 11701 aagcggaag //