| 质粒名称: | MSCB-hDot1Lwt |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 74173 |
| 存储实验室: | Yi Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pMSCB |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | hDot1L |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 5200 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | HA（插入件上的N端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | MSCB-hDot1Lwt was a gift from Yi Zhang (Addgene plasmid # 74173 ; http://n2t.net/addgene:74173 ; RRID:Addgene_74173) |
| 参考文献部分: | hDOT1L links histone methylation to leukemogenesis. Okada Y, Feng Q, Lin Y, Jiang Q, Li Y, Coffield VM, Su L, Xu G, Zhang Y. Cell. 2005 Apr 22;121(2):167-78. 10.1016/j.cell.2005.02.020 PubMed 15851025 |
买家导航
MSCBhDot1Lwt载体基本信息
MSCBhDot1Lwt载体图谱质粒图谱和多克隆位点信息
MSCBhDot1Lwt载体简介
MSCBhDot1Lwt载体序列
LOCUS MSCB-hDot1Lwt 9994 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION For packaging hDot1Lwt to retrovirus..
ACCESSION .
VERSION .
KEYWORDS MSCB-hDot1Lwt
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9994)
AUTHORS Okada Y, Feng Q, Lin Y, Jiang Q, Li Y, Coffield VM, Su L, Xu G,
Zhang Y
TITLE hDOT1L links histone methylation to leukemogenesis.
JOURNAL Cell. 2005 Apr 22;121(2):167-78.
PUBMED 15851025
REFERENCE 2 (bases 1 to 9994)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9994
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1..417
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 347..369
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 385..401
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 428..454
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter 5344..5843
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(5379..5402)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 5776..5795
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
LTR 5914..6428
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 6590..6606
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6614..6644)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6659..6680
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6797..6814)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6968..7556)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7048..7067)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7727..8587)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8350..8369
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8588..8692)
/gene="bla"
/label=AmpR promoter
enhancer 8849..9152
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 9153..9356
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 9307..9327
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 9595..9794
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
ORIGIN
1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccgga
421 attcatgtac ccatacgacg taccagatta cgctggggag aagctggagc tgagactgaa
481 gtcgcccgtg ggggctgagc ccgccgtcta cccgtggccg ctgccggtct acgataaaca
541 tcacgatgct gctcatgaaa tcatcgagac catccgatgg gtctgtgaag aaatcccgga
601 tctcaagctc gctatggaga attacgtttt aattgactat gacaccaaaa gcttcgagag
661 catgcagagg ctctgcgaca agtacaaccg tgccatcgac agcatccacc agctgtggaa
721 gggcaccacg cagcccatga agctgaacac gcggccgtcc actggactcc tgcgccatat
781 cctgcagcag gtctacaacc actcggtgac cgaccccgag aagctcaaca actacgagcc
841 cttctccccc gaggtgtacg gggagacctc cttcgacctg gtggcccaga tgattgatga
901 gatcaagatg accgacgacg acctgtttgt ggacttgggg agcggtgtgg gccaggtcgt
961 gctccaggtt gctgctgcca ccaactgcaa acatcactat ggcgtcgaga aagcagacat
1021 cccggccaag tatgcggaga ccatggaccg cgagttcagg aagtggatga aatggtatgg
1081 aaaaaagcat gcagaataca cattggagag aggcgatttc ctctcagaag agtggaggga
1141 gcgaatcgcc aacacgagtg ttatatttgt gaataatttt gcctttggtc ctgaggtgga
1201 tcaccagctg aaggagcggt ttgcaaacat gaaggaaggt ggcagaatcg tgtcctcgaa
1261 accctttgca cctctgaact tcagaataaa cagtagaaac ttgagtgaca tcggcaccat
1321 catgcgcgtg gtggagctct cgcccctgaa gggctcggtg tcgtggacgg ggaagccagt
1381 ctcctactac ctgcacacta tcgaccgcac catacttgaa aactattttt ctagtctgaa
1441 aaacccaaaa ctcagggagg aacaggaggc agcccggcgc cgccagcagc gcgagagcaa
1501 gagcaacgcg gccacgccca ctaagggccc agagggcaag gtggccggcc ccgccgacgc
1561 ccccatggac tctggtgctg aggaagagaa ggcgggagca gccaccgtga agaagccgtc
1621 tccctccaaa gcccgcaaga agaagctaaa caagaagggg aggaagatgg ctggccgcaa
1681 gcgcgggcgc cccaagaaga tgaacactgc gaaccccgag cggaagccca agaagaacca
1741 aactgcactg gatgccctgc acgctcagac cgtgtctcag acggcggcct cctcacccca
1801 ggatgcctac agatcccctc acagcccgtt ctaccagcta cctccgagcg tgcagcggca
1861 ctcccccaac ccgctgctgg tggcgcccac cccgcccgcg ctgcagaagc ttctagagtc
1921 cttcaagatc cagtacctgc agttcctggc atacacaaag accccccagt acaaggccag
1981 cctgcaggag ctgctgggcc aggagaagga gaagaacgcc cagctcctgg gtgcggctca
2041 gcagctcctc agccactgcc aggcccagaa ggaggagatc aggaggctgt ttcagcaaaa
2101 attggatgag ctgggtgtga aggcgctgac ctacaacgac ctgattcaag cgcagaagga
2161 gatctccgcc cataaccagc agctgcggga gcagtcggag cagctggagc aggacaaccg
2221 cgcgctccgc ggccagagct tgcagctgct caaggctcgc tgcgaggagc tgcagctgga
2281 ctgggccacg ctgtcgctgg agaagctgtt gaaggagaag caggccctga agagccagat
2341 ctcggagaag cagaggcact gcctggagct gcagatcagc attgtggagc tagagaagag
2401 ccagcggcag caggagctcc tgcagctcaa gtcctgtgtg ccgcctgacg acgccctgtc
2461 cctgcacctg cgtgggaagg gcgccctggg ccgcgagctg gagcctgacg ccagccggct
2521 gcacctggag ctggactgca ccaagttctc gctgcctcac ttgagcagca tgagcccgga
2581 gctctccatg aacggccagg ctgctggcta tgagctctgc ggtgtgctga gccggccttc
2641 gtcgaagcag aacacgcccc agtacctggc ctcacccctg gaccaggagg tggtgccctg
2701 tacccctagc cacgtcggcc ggccgcgcct ggagaagctg tctggcctag ccgcacccga
2761 ctacactagg ctgtccccgg ccaagattgt gctgaggcgg cacctgagcc aggaccacac
2821 ggtgcccggc aggccggctg ccagtgagct gcattcgaga gctgagcaca ccaaggagaa
2881 cggccttccc taccagagcc ccagcgtgcc tggcagcatg aagctgagcc ctcaggaccc
2941 gcggcccctg tcccctgggg ccttgcagct tgctggagag aagagcagtg agaagggcct
3001 gagagagcgc gcctacggca gcagcgggga gctcatcacc agcctgccca tcagcatccc
3061 gctcagcacc gtgcagccca acaagctccc ggtcagcatt cccctggcca gcgtggtgct
3121 gcccagccgc gccgagaggg cgaggagcac ccccagtccc gtgctgcagc cccgtgaccc
3181 ctcgtccaca cttgaaaagc agattggtgc taatgcccac ggtgctggga gcagaagcct
3241 tgccctggcc cccgcaggct tctcctacgc tggctcggtg gccatcagcg gggccttggc
3301 gggcagcccg gcctctctca cacctggagc cgagccggcc accttggatg agtcctccag
3361 ctctgggagc ctttttgcca ccgtggggtc ccgcagctcc acgccacagc accccctgct
3421 gctggcacag ccccggaact cgcttcctgc ctctcccgcc caccagctct cctccagtcc
3481 ccggcttggt ggggccgccc agggcccgtt gcccgaggcc agcaagggag acctgccctc
3541 cgattccggc ttctcagatc ctgagagtga agccaagagg aggattgtgt tcaccatcac
3601 cactggtgcg ggcagtgcca agcagtcgcc ctccagcaag cacagccccc tgaccgccag
3661 cgcccgtggg gactgtgtgc cgagccacgg gcaggacagt cgcaggcgcg gccggcggaa
3721 gcgagcatct gcggggacgc ccagcttgag cgcaggcgtg tcccccaagc gccgagccct
3781 gccgtccgtc gctggccttt tcacacagcc ttcggggtct cccctcaacc tcaactccat
3841 ggtcagtaac atcaaccagc ccctggagat tacagccatc tcgtccccgg agacctccct
3901 gaagagctcc cctgtgccct accaggacca cgaccagccc cccgtgctca agaaggagcg
3961 gcctctgagc cagaccaatg gggcacacta ctccccactc acctcagacg aggagccagg
4021 ctctgaggac gagcccagca gtgctcgaat tgagagaaaa attgcaacaa tctccttaga
4081 aagcaaatct cccccgaaaa ccttggaaaa tggtggtggc ttggcgggaa ggaagcccgc
4141 gcccgccggc gagccagtca atagcagcaa gtggaagtcc accttctcgc ccatctccga
4201 catcggcctg gccaagtcgg cggacagccc gctgcaggcc agctccgccc tcagccagaa
4261 ctccctgttc acgttccggc ccgccctgga ggagccctct gccgatgcca agctggccgc
4321 tcaccccagg aaaggctttc ccggctccct gtcgggggct gacggactca gcccgggcac
4381 caaccctgcc aacggctgca ccttcggcgg gggcctggcc gcggacctga gtttacacag
4441 cttcagtgat ggtgcttctc ttccccacaa gggccccgag gcggccggcc tgagctcccc
4501 gctgagcttc ccctcgcagc gcggcaagga gggctcggac gccaaccctt tcctgagcaa
4561 gaggcagctg gacggcctgg ctgggctgaa gggcgagggc agccgcggca aggaggcagg
4621 ggagggcggc ctaccgctgt gcgggcccac ggacaagacc ccactgctga gcggcaaggc
4681 cgccaaggcc cgggaccgcg aggtcgacct caagaatggc cacaacctct tcatctctgc
4741 ggcggccgtg cctcccggaa gcctcctcag cggccccggc ctggccccgg cggcgtcctc
4801 cgcaggcggc gcggcgtcct ccgcccagac gcaccggtcc ttcctgggcc ccttcccgcc
4861 gggaccgcag ttcgcgctcg gccccatgtc cctgcaggcc aacctcggct ccgtggccgg
4921 ctcctccgtg ctgcagtcgc tgttcagctc tgtgccggcc gccgcaggcc tggtgcacgt
4981 gtcgtccgct gccaccagac tgaccaactc gcacgccatg ggcagctttt ccggggtggc
5041 aggcggcaca gttggaggta actaggattt ctacctcaac cgcgagacct atgcaaggac
5101 ggtgtggacc aactcgcgcc cgcggcatgg tgcccgccgg cctgccgggc tcccacccct
5161 ggacggcaga ggcaaggacg gacgggagct ccactgtgaa tcggcggcac gcgccgcagg
5221 aggctgggac tggtccagtt tgtactgtcg atagttttag ataaagtatt tatcattttt
5281 taaaaagtaa aaaaaaaaaa aaaagggcgg ccgctctaga gtatccctcg agttaattct
5341 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
5401 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
5461 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
5521 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
5581 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
5641 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
5701 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
5761 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
5821 cttcctcatc tccgggcctt tcgggatctc gacctgcagc caagcttatc gataaaataa
5881 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
5941 aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga
6001 agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt
6061 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg
6121 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
6181 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
6241 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
6301 agactgcgtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
6361 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
6421 tctttcatgg gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa
6481 tattaagtaa tcctgactca attagccact gttttgaatc cacatactcc aatactcctg
6541 aaatagttca ttatggacag cgcagaaaga gctggggaga attgtgaaat tgttatccgc
6601 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
6661 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
6721 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
6781 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
6841 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
6901 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
6961 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
7021 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
7081 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
7141 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
7201 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
7261 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
7321 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
7381 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
7441 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
7501 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
7561 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
7621 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
7681 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
7741 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
7801 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
7861 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
7921 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
7981 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
8041 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
8101 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
8161 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
8221 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
8281 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
8341 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
8401 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
8461 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
8521 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
8581 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
8641 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
8701 cccgaaaagt gccaccagct ttgctcttag gagtttccta atacatccca aactcaaata
8761 tataaagcat ttgacttgtt ctatgcccta gttattaata gtaatcaatt acggggtcat
8821 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
8881 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
8941 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
9001 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
9061 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
9121 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
9181 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
9241 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
9301 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcaat
9361 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
9421 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
9481 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
9541 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
9601 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
9661 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
9721 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
9781 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
9841 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
9901 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
9961 tctgactgtg tttctgtatt tgtctgaaaa ttag
//
