| 出品公司: | Clontech |
|---|---|
| 载体名称: | pQCXIH |
| 质粒类型: | 哺乳动物细胞双顺反子逆病毒基因表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7780bp |
| 5' 测序引物及序列: | pQC-F: ACGCCATCCACGCTGTTTTGACCT |
| 3' 测序引物及序列: | pQC-R: AAGCGGCTTCGGCCAGTAACGTTA |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 筛选标记: | 潮霉素（Hygromycin） |
| 备注: | 自失活双顺反子逆病毒表达载体，含有IRES-Hygromycin元件，能够同时表达目的蛋白和潮霉素抗性筛选标记，可以用来构建稳表达细胞系。 |
| 产品目录号: | 631516 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 逆病毒 |
买家导航
pQCXIH载体质粒基本信息
pQCXIH质粒图谱载体图谱和pQCXIH载体序列质粒序列多克隆位点信息
pQCXIH质粒载体简介
pQCXIH质粒序列载体序列
LOCUS pQCXIH 7780 bp DNA circular 13-NOV-2009
COMMENT Created by Clontech Laboratories
COMMENT Cat. No. 631516
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
LTR 1..728
/label=5'\LTR\(CMV/MSV)
misc_signal 758..1567
/label=Extended\packaging\signal
LTR 4486..4911
/label=3'\MMLV\LTR\(del)
rep_origin 5798..5798
/label=pUC\origin
misc_marker complement(6558..7418)
/label=Amp(R)
rep_origin 5412..5477
/label=SV40\origin
misc_marker 2905..3939
/label=Hyg(R)
promoter 1601..2132
/label=CMV\IE\Promoter
misc_structure 2292..2881
/label=IRES
promoter 5191..5458
/label=SV40\Promoter
BASE COUNT 1802 a 2104 c 2010 g 1864 t
ORIGIN
1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga
2281 tccggaattc cgcccccccc cccccccccc ctctccctcc ccccccccta acgttactgg
2341 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
2401 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
2461 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
2521 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
2581 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
2641 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
2701 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
2761 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
2821 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
2881 agcttgccac aacccgtacc aaagatggat agatccggaa agcctgaact caccgcgacg
2941 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
3001 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
3061 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
3121 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
3181 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
3241 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
3301 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
3361 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
3421 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
3481 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
3541 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
3601 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
3661 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
3721 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
3781 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
3841 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
3901 aaccgacgcc ccagcactcg tccgagggca aaggaataga gtagatgccg accgaacaag
3961 agctgatttc gagaacgcct cagccagcaa ctcgcgcgag cctagcaagg caaatgcgag
4021 agaacggcct tacgcttggt ggcacagttc tcgtccacag ttcgctaagc tcgctcggct
4081 gggtcgcggg agggccggtc gcagtgattc aggcccttct ggattgtgtt ggtccccagg
4141 gcacgattgt catgcccacg cactcgggtg atctgactga tcccgcagat tggagatcgc
4201 cgcccgtgcc tgccgattgg gtgcagatct ctatcgaggg ctgcagcgct gcagaggccg
4261 agtgcagaac tgctccaaag ggacctcaag gctttccgag ggacactagg ctgactccat
4321 cgagccagtg tagagataag cttatcgatt agtccaattt gttaaagaca ggatatcagt
4381 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc
4441 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
4501 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
4561 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagggt cgaccctaga
4621 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
4681 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
4741 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
4801 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
4861 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc
4921 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg
4981 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
5041 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
5101 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
5161 gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
5221 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
5281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
5341 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
5401 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
5461 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttactggctt
5521 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
5581 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
5641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
5701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
5761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
5821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
5881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
5941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
6001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
6061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
6121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
6181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
6241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
6301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
6361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
6421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
6481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
6541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
6601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
6661 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
6721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
6781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
6841 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
6901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
6961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
7021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
7081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
7141 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
7201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
7261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
7321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
7381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
7441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
7501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
7561 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
7621 caagaattag cttggccatt gcatacgttg tatccatatc ataatatgta catttatatt
7681 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
7741 tcaattacgg ggtcattagt tcatagccca tatatggagt
//
