| 质粒名称: | mAzurite-16xRhoBAST |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 196347 |
| 存储实验室: | Murat Sunbul |
|
载体骨架基本信息 |
|
| 载体名称: | mAzurite-C1 |
| 完整质粒大小: | 5797 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Azurite-16xRhoBAST |
| 物种来源: | Synthetic |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | 未知(未破坏) |
| 3’克隆位点: | 未知 (未破坏) |
|
文献引用方法 |
|
| 材料和方法部分: |
mAzurite-16xRhoBAST was a gift from Murat Sunbul (Addgene plasmid # 196347 ; http://n2t.net/addgene:196347 ; RRID:Addgene_196347)
|
| 参考文献部分: | Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging. Englert D, Burger EM, Lackner J, Lampe M, Bühler B, Grün F, Schokolowski J, Nienhaus GU, Jäschke A, Sunbul M. bioRxiv 2022 10.1101/2022.10.24.513449 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mAzurite-16xRhoBAST 5797 bp ds-DNA circular SYN 15-FEB-2023
DEFINITION Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR.
ACCESSION .
VERSION .
KEYWORDS mAzurite-16xRhoBAST
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5797)
AUTHORS Englert D, Burger EM, Lackner J, Lampe M, Buhler B, Grun F,
Schokolowski J, Nienhaus GU, Jaschke A, Sunbul M
TITLE Fast-exchanging spirocyclic rhodamine probes for aptamer-based
super-resolution RNA imaging
JOURNAL bioRxiv 2022
REFERENCE 2 (bases 1 to 5797)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 15, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5797
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 133..436
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 437..640
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 591..611
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 679..688
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 685..1404
/codon_start=1
/product="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFRTAAGITHGMDELYK"
primer_bind complement(730..751)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(991..1010)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
polyA_signal 2657..2778
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2694..2713)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2748..2767
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2785..3240)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2922..2943)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3134..3153
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3267..3371
/gene="bla"
/label=AmpR promoter
promoter 3373..3730
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3396..3416)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3581..3716
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3643..3662
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3765..4559
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3819..3838)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4429..4448
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4747..4766)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4791..4838
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5167..5755
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5656..5675
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
61 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
121 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
181 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
241 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
301 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
361 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
421 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
481 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
541 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
601 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
661 ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
721 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
781 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
841 aagctgcccg tgccctggcc caccctcgtg accaccctga gccacggcgt gcagtgcttc
901 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
961 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
1021 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
1081 gaggacggca acatcctggg gcacaagctg gagtacaact tcaacagcca caacatctat
1141 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
1201 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
1261 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc
1321 aacgagaagc gcgatcacat ggtcctgctg gagttccgca ccgccgccgg gatcactcac
1381 ggcatggacg agctgtacaa gtaaggactc agatctcgag ctcaagcttc gaattctgca
1441 gtcgacatag aggaacctcc gcgaaagcgg tgaaggagag gcgcaaggtt aaccgcctca
1501 ggttcctcat aacaaggcct ccgcgaaagc ggtgaaggag cggcacaagg ttaactgccg
1561 caggccttgt atactcgaca taggaagacc ttcgcgaaag cgatgaagga gcggtgcaag
1621 gttaaccacc gcaggtcttc cataagcaga ccttcgcgaa agcgatgaag gagtggcgca
1681 aggttaaccg ccacaggtct gctatactcg acatagagga acctccgcga aagcggtgaa
1741 ggagaggcgc aaggttaacc gcctcaggtt cctcataaca aggcctccgc gaaagcggtg
1801 aaggagcggc acaaggttaa ctgccgcagg ccttgtatac tcgacatagg aagaccttcg
1861 cgaaagcgat gaaggagcgg tgcaaggtta accaccgcag gtcttccata agcagacctt
1921 cgcgaaagcg atgaaggagt ggcgcaaggt taaccgccac aggtctgcta tactcgacat
1981 agaggaacct ccgcgaaagc ggtgaaggag aggcgcaagg ttaaccgcct caggttcctc
2041 ataacaaggc ctccgcgaaa gcggtgaagg agcggcacaa ggttaactgc cgcaggcctt
2101 gtatactcga cataggaaga ccttcgcgaa agcgatgaag gagcggtgca aggttaacca
2161 ccgcaggtct tccataagca gaccttcgcg aaagcgatga aggagtggcg caaggttaac
2221 cgccacaggt ctgctatact cgacatagag gaacctccgc gaaagcggtg aaggagaggc
2281 gcaaggttaa ccgcctcagg ttcctcataa caaggcctcc gcgaaagcgg tgaaggagcg
2341 gcacaaggtt aactgccgca ggccttgtat actcgacata ggaagacctt cgcgaaagcg
2401 atgaaggagc ggtgcaaggt taaccaccgc aggtcttcca taagcagacc ttcgcgaaag
2461 cgatgaagga gtggcgcaag gttaaccgcc acaggtctgc tatactcgac gcgtgaattc
2521 actcctcgga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
2581 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
2641 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
2701 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
2761 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
2821 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
2881 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
2941 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
3001 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
3061 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
3121 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
3181 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
3241 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
3301 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
3361 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
3421 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
3481 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
3541 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
3601 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
3661 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
3721 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
3781 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
3841 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
3901 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
3961 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
4021 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
4081 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
4141 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
4201 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
4261 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
4321 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
4381 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
4441 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
4501 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
4561 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
4621 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
4681 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
4741 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
4801 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
4861 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
4921 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
4981 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
5041 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
5101 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
5161 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
5221 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
5281 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
5341 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
5401 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
5461 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
5521 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
5581 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
5641 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
5701 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
5761 cagcaacgcg gcctttttac ggttcctggc cttttgc
//
