pMAD

LOCUS       pMAD                9666 bp DNA     circular SYN 12-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Biofeng
  TITLE     Direct Submission
  JOURNAL   Exported Jul 12, 2023 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7223..7414
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    7516..7656
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7842..8430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8601..9461)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9462..9566)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaattaattc tcatgtttga cagcttatca tcgattcaca aaaaataggc acacgaaaaa
       61 caagttaagg gatgcagttt atgcatccct taacttactt attaaataat ttatagctat
      121 tgaaaagaga taagaattgt tcaaagctaa tattgtttaa atcgtcaatt cctgcatgtt
      181 ttaaggaatt gttaaattga ttttttgtaa atattttctt gtattctttg ttaacccatt
      241 tcataacgaa ataattatac ttttgtttat ctttgtgtga tattcttgat ttttttctac
      301 ttaatctgat aagtgagcta ttcactttag gtttaggatg aaaatattct cttggaacca
      361 tacttaatat agaaatatca acttctgcca ttaaaagtaa tgccaatgag cgttttgtat
      421 ttaataatct tttagcaaac ccgtattcca cgattaaata aatctcatta gctatactat
      481 caaaaacaat tttgcgtatt atatccgtac ttatgttata aggtatatta ccatatattt
      541 tataggattg gtttttagga aatttaaact gcaatatatc cttgtttaaa acttggaaat
      601 tatcgtgatc aacaagttta ttttctgtag ttttgcataa tttatggtct atttcaatgg
      661 cagttacgaa attacacctc tttactaatt caagggtaaa atggcctttt cctgagccga
      721 tttcaaagat attatcatgt tcatttaatc ttatatttgt cattatttta tctatattat
      781 gttttgaagt aataaagttt tgactgtgtt ttatattttt ctcgttcatt ataaccctct
      841 ttaatttggt tatatgaatt ttgcttatta acgattcatt ataaccactt attttttgtt
      901 tggttgataa tgaactgtgc tgattacaaa aatactaaaa atgcccatat tttttcctcc
      961 ttataaaatt agtataatta tagcacgagc tctgataaat atgaacatga tgagtgatcg
     1021 ttaaatttat actgcaatcg gatgcgatta ttgaataaaa gatatgagag atttatctaa
     1081 tttctttttt cttgtaaaaa aagaaagttc ttaaaggttt tatagttttg gtcgtagagc
     1141 acacggttta acgacttaat tacgaagtaa ataagtctag tgtgttagac tttatgaaat
     1201 ctatatacgt ttatatatat ttattatccg gaggtgtagc atgtctcatt caattttgag
     1261 ggttgccaga gttaaaggat caagtaatac aaacgggata caaagacata atcaaagaga
     1321 gaataaaaac tataataata aagacataaa tcatgaggaa acatataaaa attatgattt
     1381 gattaacgca caaaatataa agtataaaga taaaattgat gaaacgattg atgagaatta
     1441 ttcagggaaa cgtaaaattc ggtcagatgc aattcgacat gtggacggac tggttacaag
     1501 tgataaagat ttctttgatg atttaagcgg agaagaaata gaacgatttt ttaaagatag
     1561 cttggagttt ctagaaaatg aatacggtaa ggaaaatatg ctgtatgcga ctgtccatct
     1621 ggatgaaaga gtcccacata tgcactttgg ttttgtccct ttaacagagg acgggagatt
     1681 gtctgcaaaa gaacagttag gcaacaagaa agactttact caattacaag atagatttaa
     1741 tgagtatgtg aatgagaaag gttatgaact tgaaagaggc acgtccaaag aggttacaga
     1801 acgagaacat aaagcgatgg atcagtacaa gaaagatact gtatttcata aacaggaact
     1861 gcaagaagtt aaggatgagt tacagaaggc aaataagcag ttacagagtg gaatagagca
     1921 tatgaggtct acgaaaccct ttgattatga aaatgagcgt acaggtttgt tctctggacg
     1981 tgaagagact ggtagaaaga tattaactgc tgatgaattt gaacgcctgc aagaaacaat
     2041 ctcttctgca gaacggattg ttgatgatta cgaaaatatt aagagcacag actattacac
     2101 agaaaatcaa gaattaaaaa aacgtagaga gagtttgaaa gaagtagtga atacatggaa
     2161 agaggggtat cacgaaaaaa gtaaagaggt taataaatta aagcgagaga atgatagttt
     2221 gaatgagcag ttgaatgtat cagagaaatt tcaagctagt acagtgactt tatatcgtgc
     2281 tgcgagggcg aatttccctg ggtttgagaa agggtttaat aggcttaaag agaaattctt
     2341 taatgattcc aaatttgagc gtgtgggaca gtttatggat gttgtacagg ataatgtcca
     2401 gaaggtcgat agaaagcgtg agaaacagcg tacagacgat ttagagatgt agaggtactt
     2461 ttatgccgag aaaacttttt gcgtgtgaca gtccttaaaa tatacttaga gcgtaagcga
     2521 aagtagtagc gacagctatt aactttcggt tgcaaagctc taggattttt aatggacgca
     2581 gcgcatcaca cgcaaaaagg aaattggaat aaatgcgaaa tttgagatgt taattaaaga
     2641 cctttttgag gtcttttttt cttagatttt tggggttatt taggggagaa aacatagggg
     2701 ggtactacga cctcccccct aggtgtccat tgtccattgt ccaaacaaat aaataaatat
     2761 tgggttttta atgttaaaag gttgtttttt atgttaaagt gaaaaaaaca gatgttggga
     2821 ggtacagtga tagttgtaga tagaaaagaa gagaaaaaag ttgctgttac tttaagactt
     2881 acaacagaag aaaatgagat attaaataga atcaaagaaa aatataatat tagcaaatca
     2941 gatgcaaccg gtattctaat aaaaaaatat gcaaaggagg aatacggtgc attttaaaca
     3001 aaaaaagata gacagcactg gcatgctgcc tatctatgac taaattttgt taagtgtatt
     3061 agcaccgtta ttatatcatg agcgaaaatg taataaaaga aactgaaaac aagaaaaatt
     3121 caagaggacg taattggaca tttgttttat atccagaatc agcaaaagcc gagtggttag
     3181 agtatttaaa agagttacac attcaatttg tagtgtctcc attacatgat agggatactg
     3241 atacagaagg taggatgaaa aaagagcatt atcatattct agtgatgtat gagggtaata
     3301 aatcttatga acagataaaa ataattaaca gaagaattga atgcgactat tccgcagatt
     3361 gcaggaagtg tgaaaggtct tgtgagatat atgcttcaca tggacgatcc taataaattt
     3421 aaatatcaaa aagaagatat gatagtttat ggcggtgtag atgttgatga attattaaag
     3481 aaaacaacaa cagatagata taaattaatt aaagaaatga ttgagtttat tgatgaacaa
     3541 ggaatcgtag aatttaagag tttaatggat tatgcaatga agtttaaatt tgatgattgg
     3601 ttcccgcttt tatgtgataa ctcggcgtat gttattcaag aatatataaa atcaaatcgg
     3661 tataaatctg accgatagat tttgaattta ggtgtcacaa gacactcttt tttcgcacca
     3721 gcgaaaactg gtttaagccg actgcgcaaa agacataatc gataagcttt aatgcggtag
     3781 tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct
     3841 catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt
     3901 actgccgggc ctcttgcggg atcgaggcct ttagcatatt atgttgccaa ctgtcggaac
     3961 gagacttctc taaaccttcc cggcttcatc atgctctctt aacactgcta catcaacccc
     4021 ttgaatcctc aattcacctc ctcgaaaaca tttcccatca atcatattct ggtatatctt
     4081 atcttctggc agtgacagcg tcacttcgta atcattatga ttgatgataa tcaaatactt
     4141 ccattcatca gtctctcttt gttgcacctc tacattttca gctacttcaa gaatgggatt
     4201 aatatgatgt ttagcgaaaa cctgttctaa aagcctgcct aaataattac tatctggata
     4261 agtaccgacg taaatcccct ctcctttacc gtagcagtta cgtgtaaccg ccggaagtcc
     4321 tgcataccaa tcccccttaa atgtcgctag aggttctgcc ccttctaatc ggattatgtc
     4381 cgcccacgtc gtacaatcat attccccatc gttactatat attttgttta cctttgtttc
     4441 tgggtatggt acaaattcct caacaaaaat ccccaaaata tctcgcagag gaccaggata
     4501 tccgcctaga tgtacacggt cattttcatc tacaatgcca ctgaagaaac tgacaatcaa
     4561 agtgccaccg ttagcaacaa attgccgtaa gttttcatct tctccctctt taaccatata
     4621 taacattgga gcaataacta ctttgtattt tgttagatca tcagatggcc ttacaaaatc
     4681 gacagcaata ttacgtttat ataattccct ataataagct tcaactatag gaatatatct
     4741 tagtttatta tgtggtttgg aacttagttc gacagcccac cagttttccc aatcaaaaat
     4801 gatcgcgacc tctgccttga ttctagatcc gaccaaacaa tctaactttt tcagctcttg
     4861 ccctaactgt gtaacttccc tataaattct attattctcg ttcaaaaagt ggggcaccat
     4921 tgcaccgtgg aatttttcag ctcctgctct actttgacgc cactggaaaa acataatacc
     4981 atctgcacca cgggcaatag ttgcataact ccatagacgc attacacctg gcggttttgg
     5041 aacattaata tcgcgccagt taacatgtga ggttacctgc tccatcaaaa taaacggttg
     5101 accttttctt aaactacgca taaggtcatt catcatggcg tgctgaattg gcaagccctc
     5161 tctgggatca ggatatgagt cccatgtcac aatatctaca tgctgagccc attgaaaata
     5221 gtttaacggt ttgaatgaac ccatgaaatt agttgatact ggaatatctg gtgttacctc
     5281 acgtaaaatt tccttttctg ttaaaaacaa cttgagaatt gagtcattca taaaacggta
     5341 gtaatcaagt tcttgggatg gattaataaa agttggtgcc tttctagggg gattaatttc
     5401 atcccaatga ttgtatcgct gtccccaaaa gtttgtaccc caacgttcat ttaattcatc
     5461 gattgtttta tatctttcct ttagccactt tctaaacgcg acagcacaat tctcacaaaa
     5521 acacttggaa acgtgacatg catactcatt attaacatgc cacattttga gtgccggatg
     5581 atttttatac cgttctgcta tagccctcac aagtctcttt atgtgcgtaa ttaattgagg
     5641 atgattagga caataatgtt gtctactgcc aaacgagaga atgacaccgc tttcatcgat
     5701 cggcaaagaa tctggatact tttttacaaa ccaagctgga gtagttgcag tcgccgtccc
     5761 caagttaata taaacaccgt ggtcatatag tatatctata accttgtcta gccattcaaa
     5821 gtcgaacact ccatcagacg gttcgatctt gctccaactg aaaatcccta aagatactaa
     5881 attcaccccc gctttttgca tcaacttagc atcttcatac caaatttcct ctggccattg
     5941 ctctgggtta taatctcctc cgtaacaaat tgaggataac acattcataa ttattccccc
     6001 tagctaattt tcgtttaatt ataaattaag ttaaaattta ggtacctacg taggatcgat
     6061 ccgatcctta attatattat agtcccaata taatcattta tcaactcttt tacacttaaa
     6121 tttcctaata tttgtaaata tttttttgaa taaaaaaatc tagctaatgt tacgttacac
     6181 attaactaga cagatctatc gatgcatgcc atggtacccg ggagctcgaa ttctagaagc
     6241 ttctgcagac gcgtcgacgt catatggatc cgatatcgcc cgacgcgagg ctggatggcc
     6301 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     6361 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     6421 accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     6481 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     6541 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
     6601 tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga
     6661 atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca
     6721 cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
     6781 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     6841 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     6901 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
     6961 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
     7021 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
     7081 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
     7141 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatt
     7201 cccccttaca cggaggcatc aagtgaccaa acaggaaaaa accgccctta acatggcccg
     7261 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
     7321 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
     7381 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     7441 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     7501 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     7561 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     7621 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     7681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8281 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8701 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8881 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     8941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     9241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     9601 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     9661 cttcaa
//