出品公司: | ATCC |
---|---|
载体名称: | YCplac22 |
质粒类型: | 酿酒酵母表达载体;中心粒载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | lacZ |
载体大小: | 4854 bp |
5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | TRP1 |
表达菌株: | 酿酒酵母、白色念珠菌 |
备注: | -- |
产品目录号: | 87585 |
稳定性: | 稳表达 |
组成型/诱导型: | 诱导型 |
病毒/非病毒: | 非病毒 |
买家导航
YCplac22载体质粒基本信息
YCplac22质粒图谱载体图谱和YCplac22载体序列质粒序列多克隆位点信息
YCplac22质粒载体简介
YCplac22质粒序列载体序列
LOCUS YCplac22 4854 bp DNA SYN DEFINITION YCplac22 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4854 /organism="YCplac22" /mol_type="other DNA" promoter 142..171 /label="lac_promoter" misc_feature 185..207 /label="M13_pUC_rev_primer" promoter 206..224 /label="M13_reverse_primer" misc_feature 287..442 /label="lacZ_a" promoter complement(290..306) /label="M13_forward20_primer" misc_feature complement(299..321) /label="M13_pUC_fwd_primer" misc_feature 504..1356 /label="CEN4" gene complement(2066..2740) /label="TRP1" /gene="TRP1" CDS complement(2066..2740) /label="ORF frame 3" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" promoter 2983..3011 /label="AmpR_promoter" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" gene 3053..3913 /label="Ampicillin" /gene="Ampicillin" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK*" CDS 3053..3913 /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 4068..4687 /label="pBR322_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc 241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg 301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt 481 gcggtatttc acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat 541 tgtcatatta ctagttggtg tggaagtcca tatatcggtg atcaatatag tggttgacat 601 gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca 661 aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa 721 gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg 781 tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata 841 catataatac acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa 901 attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg 961 taaaagttca ctttagcata catatattac acgagccaga aattgtaact tttgcctaaa 1021 atcacaaatt gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt 1081 aaaaatttaa aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat 1141 tgtttatgat taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga 1201 aaaaaagcac tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac 1261 agaaaatagg cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc 1321 acagttttac agtaaataag tatcacctct tagagttcga tgataagctg tcaaacatga 1381 gaattaattc cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg 1441 tggggtaagt gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt 1501 atcaacatat agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag 1561 gctgatggtg tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac 1621 tgtcagcgta gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata 1681 aagtttttac agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag 1741 gtatgtacat gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag 1801 gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt 1861 gattccattg cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa 1921 tcaatcaaaa agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta 1981 tgtttagatc ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact 2041 taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt 2101 tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc 2161 catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat 2221 gtaagctctc ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa 2281 gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg 2341 aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca 2401 aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatatcaa 2461 tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga aacacgccaa 2521 ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt gaaattttcc 2581 ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata cccagcaagt 2641 cagcatcgga atctagtgca cattctgcgg cctctgtgct ctgcaagccg caaactttca 2701 ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct gcctttgtgt 2761 gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct tggccctctc 2821 cttttctttt ttcgaccgaa ttaattcttg aagacgaaag ggcctcgtga tacgcctatt 2881 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg 2941 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 3001 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 3061 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 3121 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 3181 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 3241 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 3301 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 3361 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 3421 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 3481 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 3541 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 3601 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 3661 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 3721 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 3781 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 3841 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 3901 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 3961 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 4021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 4081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 4141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 4201 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 4261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 4381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 4441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 4501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 4561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 4621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 4681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 4741 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 4801 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaga //