| 出品公司: | Hegemann Lab |
|---|---|
| 载体名称: | pUG66 |
| 质粒类型: | 酵母菌株基因敲除载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3580 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | Bleomycin 博来霉素 |
| 产品目录号: | -- |
| 稳定性: | 稳定 |
| 组成型: | 组成型表达 |
| 病毒/非病毒: | 非病毒 |
| 备注: |
pUG66载体是大肠杆菌-酵母穿梭载体，在大肠杆菌中抗性是氨苄。pUG66载体质粒含有来自Tn5载体质粒的Ble基因，使得载体质粒在酵母菌中呈现出博来霉素抗性。 pUG66载体在筛选基因Ble两侧含有loxp基因，通过表达cre重组酶，可以利用loxp基因将Ble s筛选基因删除。 pUG66载体可以作为酵母基因敲除的一个质粒PCR模板。代表性的PCR片段的整合插入宿主菌，使得基因敲除突变子能够进行顺利筛选。 |
买家导航
pUG66载体质粒基本信息
pUG66质粒图谱载体图谱和pUG66载体序列质粒序列多克隆位点信息
pUG66质粒载体简介
pUG66质粒序列载体序列
LOCUS pUG66 3580 bp DNA circular SYN 11-MAR-2002
DEFINITION PCR template vector pUG66, complete sequence.
ACCESSION AF298794
VERSION AF298794.1 GI:11344912
KEYWORDS .
SOURCE PCR template vector pUG66
ORGANISM PCR template vector pUG66
other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
REFERENCE 1 (bases 1 to 3580)
AUTHORS Gueldener,U., Heinisch,J., Koehler,G.J., Voss,D. and Hegemann,J.H.
TITLE A second set of loxP marker cassettes for Cre-mediated multiple
gene knockouts in budding yeast
JOURNAL Nucleic Acids Res. 30 (6), E23 (2002)
PUBMED 11884642
REFERENCE 2 (bases 1 to 3580)
AUTHORS Gueldener,U., Heinisch,J.H., Voss,D., Koehler,G. and Hegemann,J.H.
TITLE Direct Submission
JOURNAL Submitted (23-AUG-2000) Institut fuer Mikrobiologie,
Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
40225, Germany
FEATURES Location/Qualifiers
source 1..3580
/organism="PCR template vector pUG66"
/mol_type="other DNA"
/db_xref="taxon:142963"
/note="derived from Saccharomyces cerevisiae"
misc_feature 54..87
/note="loxP site"
regulatory 88..484
/regulatory_class="promoter"
/note="from TEF"
gene 486..866
/gene="ble"
CDS 486..866
/gene="ble"
/codon_start=1
/transl_table=11
/product="bleomycin resistance protein"
/protein_id="AAG34545.1"
/db_xref="GI:11344913"
regulatory 873..1131
/regulatory_class="terminator"
/note="from TEF"
misc_feature 1132..1165
/note="loxP site"
gene complement(2254..3114)
/gene="AmpR"
CDS complement(2254..3114)
/gene="AmpR"
/codon_start=1
/transl_table=11
/product="ampicillin resistance protein"
/protein_id="AAG34546.1"
/db_xref="GI:11344914"
ORIGIN
1 agaacgcggc cgccagctga agcttcgtac gctgcaggtc gacaaccctt aatataactt
61 cgtataatgt atgctatacg aagttattag gtctagagat ctgtttagct tgcctcgtcc
121 ccgccgggtc acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac
181 gtgcgcagct caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat
241 gtataatcat ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc
301 tcctcgctgc agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc
361 cacgccgcgc ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt
421 catatacttc cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa
481 caaccatggc cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccacggccg
541 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
601 agcgcgggga tctcaagctg gagttcttcg cccaccccgg gctcgatccc ctcgcgagtt
661 ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg
721 tcggcatcca ggaaaccagc agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt
781 ggggaggcac gatggccgct ttggtcgacc cggacgggac gctcctgcgc ctgatacaga
841 acgaattgct tgcaggcatc tcatgatcag tactgacaat aaaaagattc ttgttttcaa
901 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
961 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
1021 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg
1081 atactaacgc cgccatccag tgtcgaaaac gagctctcga gaacccttaa tataacttcg
1141 tataatgtat gctatacgaa gttattaggt gatatcagat ccactagtgg cctatgcggc
1201 cgcggatctg ccggtctccc tatagtgagt cgtattaatt tcgataagcc aggttaacct
1261 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
1321 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
1381 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
1441 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
1501 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
1561 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
1621 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
1681 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
1741 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
1801 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
1861 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
1921 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
1981 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
2041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
2101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
2161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
2221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
2281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
2341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
2401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
2461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
2521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
2581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
2641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
2701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
2761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
2821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
2881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
2941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
3001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
3061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
3121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
3181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
3241 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
3301 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
3361 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
3421 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
3481 tgtactgaga gtgcaccata tggacatatt gtcgttagaa cgcggctaca attaatacat
3541 aaccttatgt atcatacaca tacgatttag gtgacactat
//
