| 质粒名称: | S-HBsAg |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 103013 |
| 存储实验室: | Wang-Shick Ryu |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 6119 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HBV Small envelope protein |
| 物种来源: | Hepatitis B virus |
| 插入大小: | 681 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | S-HBsAg was a gift from Wang-Shick Ryu (Addgene plasmid # 103013 ; http://n2t.net/addgene:103013 ; RRID:Addgene_103013) |
| 参考文献部分: | Hepatitis B virus polymerase blocks pattern recognition receptor signaling via interaction with DDX3: implications for immune evasion. Wang H, Ryu WS. PLoS Pathog. 2010 Jul 15;6(7):e1000986. doi: 10.1371/journal.ppat.1000986. 10.1371/journal.ppat.1000986 PubMed 20657822 |
买家导航
SHBsAg载体基本信息
SHBsAg载体图谱质粒图谱和多克隆位点信息
SHBsAg载体简介
SHBsAg载体序列
LOCUS S-HBsAg 6118 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses HBV Small envelope glycoprotein in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS S-HBsAg
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6118)
AUTHORS Wang H, Ryu WS
TITLE Hepatitis B virus polymerase blocks pattern recognition receptor
signaling via interaction with DDX3: implications for immune
evasion.
JOURNAL PLoS Pathog. 2010 Jul 15;6(7):e1000986. doi:
10.1371/journal.ppat.1000986.
PUBMED 20657822
REFERENCE 2 (bases 1 to 6118)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6118
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 956..1636
/codon_start=1
/product="major/small (S) envelope protein of hepatitis B
virus"
/label=HBsAg (S)
/note="hepatitis B surface antigen"
/translation="MENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGT
TVCLGQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGML
PVCPLIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLW
EWASARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCL
WVYI"
promoter complement(1671..1689)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(1672..1689)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(1709..1726)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1715..1939
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1985..2413
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2072..2091)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2282..2303
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2422..2442)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2427..2756
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2607..2742
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2669..2688
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2823..3617
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2877..2896)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3487..3506
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 3791..3912
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3828..3847)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3882..3901
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3961..3977)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3961..3977)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3974..3996)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3985..4001
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4009..4039)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4054..4075
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4192..4209)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4363..4951)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4443..4462)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5122..5982)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5745..5764
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5983..6087)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atcccatgga
961 gaacatcaca tcaggattcc taggacccct tctcgtgtta caggcggggt ttttcttgtt
1021 gacaagaatc ctcacaatac cgcagagtct agactcgtgg tggacttctc tcaattttct
1081 agggggaact accgtgtgtc ttggccaaaa ttcgcagtcc ccaacctcca atcactcacc
1141 aacctcttgt cctccaactt gtcctggtta tcgctggatg tgtctgcggc gttttatcat
1201 cttcctcttc atcctgctgc tatgcctcat cttcttgttg gttcttctgg actatcaagg
1261 tatgttgccc gtttgtcctc taattccagg atcctcaaca accagcacgg gaccatgccg
1321 gacctgcatg actactgctc aaggaacctc tatgtatccc tcctgttgct gtaccaaacc
1381 ttcggacgga aattgcacct gtattcccat cccatcatcc tgggctttcg gaaaattcct
1441 atgggagtgg gcctcagccc gtttctcctg gctcagttta ctagtgccat ttgttcagtg
1501 gttcgtaggg ctttccccca ctgtttggct ttcagttata tggatgatgt ggtattgggg
1561 gccaagtctg tacagcatct tgagtccctt tttaccgctg ttaccaattt tcttttgtct
1621 ttgggtatac atttaagcgg ccgctcgagc atgcatctag agggccctat tctatagtgt
1681 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
1741 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
1801 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
1861 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
1921 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
1981 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
2041 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
2101 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
2161 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
2221 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
2281 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
2341 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
2401 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
2461 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
2521 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
2581 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
2641 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
2701 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
2761 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
2821 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
2881 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
2941 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
3001 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
3061 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
3121 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
3181 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
3241 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
3301 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
3361 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
3421 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
3481 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
3541 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
3601 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
3661 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
3721 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
3781 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
3841 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
3901 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
3961 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
4021 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
4081 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
4141 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
4201 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
4261 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
4321 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
4381 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
4441 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
4501 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
4561 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
4621 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
4681 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
4741 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
4801 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
4861 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
4921 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
4981 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
5041 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
5101 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
5161 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
5221 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
5281 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
5341 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
5401 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
5461 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
5521 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
5581 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
5641 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
5701 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
5761 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
5821 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
5881 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
5941 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
6001 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
6061 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
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