| 质粒名称: | M-HBsAg |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 103012 |
| 存储实验室: | Wang-Shick Ryu |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 6284 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HBV Medium envelope protein |
| 物种来源: | Hepatitis B virus |
| 插入大小: | 846 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | M-HBsAg was a gift from Wang-Shick Ryu (Addgene plasmid # 103012 ; http://n2t.net/addgene:103012 ; RRID:Addgene_103012) |
| 参考文献部分: | Hepatitis B virus polymerase blocks pattern recognition receptor signaling via interaction with DDX3: implications for immune evasion. Wang H, Ryu WS. PLoS Pathog. 2010 Jul 15;6(7):e1000986. doi: 10.1371/journal.ppat.1000986. 10.1371/journal.ppat.1000986 PubMed 20657822 |
买家导航
MHBsAg载体基本信息
MHBsAg载体图谱质粒图谱和多克隆位点信息
MHBsAg载体简介
MHBsAg载体序列
LOCUS M-HBsAg 6283 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses HBV Medium envelope glycoprotein in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS M-HBsAg
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6283)
AUTHORS Wang H, Ryu WS
TITLE Hepatitis B virus polymerase blocks pattern recognition receptor
signaling via interaction with DDX3: implications for immune
evasion.
JOURNAL PLoS Pathog. 2010 Jul 15;6(7):e1000986. doi:
10.1371/journal.ppat.1000986.
PUBMED 20657822
REFERENCE 2 (bases 1 to 6283)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6283
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 956..1801
/codon_start=1
/product="middle (pre-S2/S) envelope protein of hepatitis B
virus"
/label=HBsAg (pre-S2/S)
/note="hepatitis B surface antigen"
/translation="MQWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVLTTASPLSSI
FSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCL
GQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCP
LIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWAS
ARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI
"
promoter complement(1836..1854)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(1837..1854)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(1874..1891)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1880..2104
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2150..2578
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2237..2256)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2447..2468
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2587..2607)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2592..2921
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2772..2907
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2834..2853
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2988..3782
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3042..3061)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3652..3671
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 3956..4077
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3993..4012)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4047..4066
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4126..4142)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4126..4142)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4139..4161)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4150..4166
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4174..4204)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4219..4240
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4357..4374)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4528..5116)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4608..4627)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5287..6147)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5910..5929
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6148..6252)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atcccatgca
961 gtggaattcc acaaccttcc accaaactct gcaagatccc agagtgagag gcctgtattt
1021 ccctgctggt ggctccagtt caggaacagt aaaccctgtt ctgactactg cctctccctt
1081 atcgtcaatc ttctcgagga ttggggaccc tgcgctgaac atggagaaca tcacatcagg
1141 attcctagga ccccttctcg tgttacaggc ggggtttttc ttgttgacaa gaatcctcac
1201 aataccgcag agtctagact cgtggtggac ttctctcaat tttctagggg gaactaccgt
1261 gtgtcttggc caaaattcgc agtccccaac ctccaatcac tcaccaacct cttgtcctcc
1321 aacttgtcct ggttatcgct ggatgtgtct gcggcgtttt atcatcttcc tcttcatcct
1381 gctgctatgc ctcatcttct tgttggttct tctggactat caaggtatgt tgcccgtttg
1441 tcctctaatt ccaggatcct caacaaccag cacgggacca tgccggacct gcatgactac
1501 tgctcaagga acctctatgt atccctcctg ttgctgtacc aaaccttcgg acggaaattg
1561 cacctgtatt cccatcccat catcctgggc tttcggaaaa ttcctatggg agtgggcctc
1621 agcccgtttc tcctggctca gtttactagt gccatttgtt cagtggttcg tagggctttc
1681 ccccactgtt tggctttcag ttatatggat gatgtggtat tgggggccaa gtctgtacag
1741 catcttgagt ccctttttac cgctgttacc aattttcttt tgtctttggg tatacattta
1801 agcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga
1861 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
1921 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
1981 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
2041 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
2101 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
2161 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
2221 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
2281 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
2341 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
2401 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
2461 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
2521 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
2581 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
2641 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
2701 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
2761 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
2821 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
2881 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
2941 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
3001 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
3061 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
3121 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
3181 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
3241 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
3301 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
3361 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
3421 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
3481 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
3541 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
3601 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
3661 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
3721 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
3781 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
3841 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
3901 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
3961 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
4021 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
4081 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
4141 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
4201 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
4261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
4321 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
4381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
4441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
4561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
4621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
4741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
4921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
4981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
5041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
5101 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
5161 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
5221 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
5281 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
5341 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
5401 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
5461 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
5521 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
5581 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
5641 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
5701 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
5761 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
5821 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
5881 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
5941 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
6001 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
6061 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
6121 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
6181 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
6241 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
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