| 质粒名称: | pEBTet_NAD-Snifit-cyto |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 113914 |
| 存储实验室: | Kai Johnsson |
|
载体骨架基本信息 |
|
| 载体名称: | pEBTet |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 11448 bp |
| 完整质粒大小: | 13797 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | NAD-Snifit,hSPR(D41W42)-HaloTag7-Pro30-SNAPtag |
| 物种来源: | Synthetic |
| 插入大小: | 2349 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV TetO2 |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV_Fwd |
| 3' 测序引物 | BGH_Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pEBTet_NAD-Snifit-cyto was a gift from Kai Johnsson (Addgene plasmid # 113914 ; http://n2t.net/addgene:113914 ; RRID:Addgene_113914) |
| 参考文献部分: | Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K. Elife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638. 32638 [pii] PubMed 29809136 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEBTet_NAD-Snifit-cyto 13797 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Plasmid encoding the cytosolic localization of the FRET
semi-synthetic biosensor measuring NAD+ concentration in mammalian
cells.
ACCESSION .
VERSION .
KEYWORDS pEBTet_NAD-Snifit-cyto
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13797)
AUTHORS Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K
TITLE Semisynthetic biosensors for mapping cellular concentrations of
nicotinamide adenine dinucleotides.
JOURNAL Elife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
PUBMED 29809136
REFERENCE 2 (bases 1 to 13797)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13797
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 807..1691
/codon_start=1
/product="modified bacterial dehalogenase that forms
covalent bonds with chloroalkane derivatives"
/label=HaloTag(R)
/translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
SEIARWLSTLEISG"
primer_bind complement(2422..2439)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2428..2539
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3080..4869
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind complement(7533..7551)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7619..7723
/gene="bla"
/label=AmpR promoter
CDS 7724..8584
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7942..7961)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8755..9343
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9244..9263
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(9532..9552)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9550..9847
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind 9779..9798
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 9924..10523
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(9924..9943)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 10420..10440
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
polyA_signal 10614..10748
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10886..10905)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 11182..11443
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
intron 11560..12132
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind 12140..12159
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind 12187..12206
/label=T7
/note="T7 promoter, forward primer"
promoter 12187..12205
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 12216..12833
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
primer_bind complement(12289..12308)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
polyA_signal 12878..12999
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
enhancer 13054..13433
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 13434..13637
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 13588..13608
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 13639..13657
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 13660..13678
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 13688..13712
/label=LNCX
/note="Human CMV promoter, forward primer"
ORIGIN
1 agcttaccac catggagggc gggctggggc gtgctgtgtg cttgctgacc ggggcctccc
61 gcggcttcgg ccggacgctg gccccgctcc tggcctcgct gctgtcgccc ggctccgtgc
121 ttgtccttag cgactggaac gacgaggcac tgcgccagct ggaggccgag ctgggcgccg
181 agcggtctgg cctgcgcgtg gtgcgggtgc ccgccgacct gggcgccgag gccggcttgc
241 agcagctgct cggcgccctg cgcgagctcc cccggcccaa ggggctgcag cgactgctgc
301 ttatcaacaa cgcgggctct cttggggatg tgtccaaagg cttcgtggac ctgagtgact
361 ccactcaagt gaacaactac tgggcactga acttgacctc catgctctgc ctgacttcca
421 gcgtcctgaa ggccttcccg gacagtcctg gcctcaacag aaccgtggtt aacatctcgt
481 ccctctgtgc cctgcaacct ttcaaaggct gggcgctgta ctgtgcagga aaggctgctc
541 gtgatatgct gttccaggtc ctggcgctgg aggaacctaa tgtgagggtg ctgaactatg
601 ccccaggtcc tctggacaca gacatgcagc agttggcccg ggagacctcc gtggacccag
661 acatgcgaaa agggctgcag gagctgaagg caaaggggaa gctggtggat tgcaaggtgt
721 cagcccagaa actgctgagc ttactggaaa aggacgagtt caagtctgga gcccacgtgg
781 acttctatga caaagaattc ggatccgaaa tcggtactgg ctttccattc gacccccatt
841 atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc
901 ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc
961 atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca
1021 aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag
1081 ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc
1141 actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccgcc
1201 ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca
1261 ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc
1321 cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc
1381 tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg
1441 agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg
1501 tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc
1561 gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc
1621 tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg tctactctgg
1681 agatttccgg tagcggccgc cctcctcctc cgccaccgcc accaccgccg ccgccgccac
1741 caccacctcc tccgcctcct ccaccaccac cgccaccgcc tccaccacct ggcggccgct
1801 ctcggtctct cgagatggat aaagactgcg aaatgaaacg cactaccctg gacagcccgc
1861 tgggtaaact ggaactgagc ggttgcgagc agggcctgca cgaaatcatt ttcctgggca
1921 aaggtacgag cgctgcggat gcggtcgaag taccggctcc ggctgcggtt ctgggtggcc
1981 cggagccgct gatgcaggct accgcatggc tgaacgcata ttttcaccag ccagaggcga
2041 ttgaagagtt ccctgtaccg gccctgcatc acccggtatt tcagcaggaa tcctttaccc
2101 gtcaggtact gtggaaactg ctgaaagtag tgaaattcgg cgaggtgatc tcctattccc
2161 atctggcggc gctggctggc aacccagcgg caactgcggc ggtgaaaacc gctctgtctg
2221 gtaatccggt gccaattctg atcccgtgcc accgtgtggt tcagggcgac ctggacgtag
2281 gtggctacga aggcggcctg gctgtgaaag aatggctgct ggcgcacgaa ggtcaccgtc
2341 tgggtaaacc aggcctgggc taaagctagc ggcgcgcccg gccgctcgag tctagagggc
2401 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
2461 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
2521 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
2581 tggggcagga cagcaagggg gaggattggg aagacaatag caggctagag gatcgatccc
2641 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
2701 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
2761 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
2821 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
2881 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
2941 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
3001 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
3061 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
3121 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
3181 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
3241 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
3301 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
3361 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
3421 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
3481 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
3541 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
3601 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
3661 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
3721 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
3781 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
3841 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
3901 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
3961 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
4021 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
4081 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
4141 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
4201 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
4261 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
4321 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
4381 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
4441 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
4501 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
4561 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
4621 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
4681 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
4741 gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta gtagagtggg
4801 agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc gctgcttgtc
4861 cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc taaagccgtc
4921 gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg
4981 agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg ttgggaggac
5041 gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga tgtctactgg
5101 ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg gcgtctccta
5161 acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct tcatctccgt
5221 catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac cagggaggca
5281 aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta ggagcgggct
5341 ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata tgagtttgta
5401 aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt gggccgggtc
5461 caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt ggaatagcaa
5521 gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat acgaacacac
5581 cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc aggagagctc
5641 ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca cgatgctttt
5701 ccaaaccacc ctcctttttt gcgccctgcc tccatcaccc tgaccccggg gtccagtgct
5761 tgggccttct cctgggtcat ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg
5821 ctcaccatct gggccacctt cttggtggta ttcaaaataa tcggcttccc ctacagggtg
5881 gaaaaatggc cttctacctg gagggggcct gcgcggtgga gacccggatg atgatgactg
5941 actactggga ctcctgggcc tcttttctcc acgtccacga cctctccccc tggctctttc
6001 acgacttccc cccctggctc tttcacgtcc tctaccccgg cggcctccac tacctcctcg
6061 accccggcct ccactacctc ctcgaccccg gcctccactg cctcctcgac cccggcctcc
6121 acctcctgct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc
6181 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc
6241 ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
6301 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
6361 tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc
6421 ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
6481 ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc
6541 tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc
6601 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
6661 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc
6721 tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
6781 tcctgctcct gcccctcctg ctcctgcccc tcccgctcct gctcctgctc ctgttccacc
6841 gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga caccatctct
6901 atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg
6961 tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc cactgccgcc
7021 ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag gtcctgtacc
7081 tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt tattagacga
7141 cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc ccaccctcat
7201 ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc tccgaaccat
7261 cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca tcctcaagat
7321 ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg ctggacggta
7381 gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac agagatgcta
7441 ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc gatgataagc
7501 tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
7561 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
7621 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
7681 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
7741 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
7801 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
7861 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
7921 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
7981 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
8041 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
8101 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
8161 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
8221 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
8281 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
8341 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
8401 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
8461 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
8521 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
8581 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
8641 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
8701 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
8761 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
8821 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
8881 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
8941 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
9001 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
9061 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
9121 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
9181 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
9241 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
9301 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
9361 ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac
9421 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
9481 cataatgggg aaggccatcc agcctcgcgt cgaactagat gatccggctg tggaatgtgt
9541 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
9601 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
9661 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
9721 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
9781 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
9841 tttttggagg gtgaccgcca cgaccggtgc cgccaccatc ccctgaccca cgcccctgac
9901 ccctcacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc
9961 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
10021 cgcgccacac cgtcgacccc gaccgccaca tcgaacgcgt caccgagctg caagaactct
10081 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
10141 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
10201 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
10261 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
10321 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
10381 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
10441 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
10501 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
10561 gcgcacgacc cggtccgacg cggcccgacg ggtccgaggg gggtcgacct cgaaacttgt
10621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
10681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
10741 tctggatctg atccgaaccc cttcctcgac caattctcat gtttgacagc ttatcatcgc
10801 agatccgggc aacgttgttg ccattgctcg accaattctc atgtttgaca gcttatcatc
10861 gcagatccgt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
10921 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
10981 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
11041 cgctgcttcg cgatgtacgg gccagatata cgcgtatctg aggggactag ggtgtgttta
11101 ggcgaaaagc ggggcttcgg ttgtacgcgg ttaggagtcc cctcaggata tagtagtttc
11161 gcttttgcat agggaggggg aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat
11221 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
11281 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
11341 gattggacga accactgaat tccgcattgc agagatattg tatttaagtg cctagctcga
11401 tacaataaac gccatttgac cattcaccac attggtgtgc acctccaagc tggccgggga
11461 tcctctaggg cctctgagct attccagaag tagtgaagag gcttttttgg aggcctaggc
11521 ttttgcaaaa agctccggat cgatcctgag aacttcaggg tgagtttggg gacccttgat
11581 tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg
11641 gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt
11701 ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct
11761 gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt
11821 tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata
11881 ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga
11941 atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac
12001 atcctggtca tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg
12061 aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc
12121 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
12181 gaattgtaat acgactcact atagggcgaa ttgatatgtc tagattagat aaaagtaaag
12241 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
12301 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
12361 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
12421 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
12481 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
12541 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
12601 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
12661 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
12721 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
12781 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgcgt
12841 acagcggatc ccgggaattc agatcttatt aaagcagaac ttgtttattg cagcttataa
12901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
12961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggt cgaatccatg
13021 gcgcagaact ggtaggtatg gaagatccgc gttgacattg attattgact agttattaat
13081 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
13141 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
13201 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
13261 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
13321 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
13381 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
13441 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
13501 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
13561 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
13621 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc
13681 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
13741 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaactta
//
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